Stable Non‐Covalent Large Area Patterning of Inert Teflon‐AF Surface: A New Approach to Multiscale Cell Guidance

Abstract Micro‐ and nano‐patterning of cell adhesion proteins is demonstrated to direct the growth of neural cells, viz. human neuroblastoma SHSY5Y, at precise positions on a strongly antifouling substrate of technolological interest. We adopt a soft‐lithographic approach with oxygen plasma modified PDMS stamps to pattern human laminin on Teflon‐AF films. These patterns are based on the interplay of capillary forces within the stamp and non‐covalent intermolecular and surface interactions. Remarkably, they remain stable for several days upon cell culture conditions. The fabrication of substrates with adjacent antifouling and adhesion‐promoting regions allows us to reach absolute spatial control in the positioning of neuroblastoma cells on the Teflon‐AF films. This patterning approach of a technologically‐relevant substrate can be of interest in tissue engineering and biosensing.