Composite scaffolds based on mesoporous bioactive glasses and Sr,Mg-doped calcium phosphates as cell carriers for bone tissue engineering
Source abstract
Abstract
Scaffolds in bone tissue engineering (BTE) function as temporary 3D support for cells, recreating a bone-like microenvironment that promotes their adhesion, proliferation, and differentiation. Calcium phosphate (CaP) ceramics are widely used in orthopedics owing to their similarity to biological apatite, while ion doping and bioactive glass addition further enhance biological activity and mechanical stability. Here, composite scaffolds were fabricated from Sr,Mg-doped CaP combined with pristine or Sr,Mg-doped mesoporous bioactive glass nanoparticles (MBGNs). MBGNs incorporation promoted α -tricalcium phosphate phase formation and significantly improved the compressive strength via reduced microporosity through liquid-phase sintering without compromising the macroporosity. Bioactivity was confirmed under dynamic perfusion. Obtained scaffolds supported attachment and growth of human bone marrow stromal cells and endothelial cells, with enhanced pro-angiogenic properties. These results highlight Sr,Mg-doped CaP/MBGN composites as a mechanically reinforced, osteogenic and pro-angiogenic platform for BTE.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Magnesium Oxide Properties and Applications · Calcium Carbonate Crystallization and Inhibition
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (75)
Show source-provided references
1. 3D bioactive composite scaffolds for bone tissue engineering
2. Reconstruction of large skeletal defects: current clinical therapeutic strategies and future directions using 3D printing10.3389/fbioe.2020.00061 ↗
3. Bone regeneration strategies: engineered scaffolds, bioactive molecules and stem cells current stage and future perspectives10.1016/j.biomaterials.2018.07.017 ↗
4. Bifunctional scaffolds for tumor therapy and bone regeneration: synergistic effect and interplay between therapeutic agents and scaffold materials
5. Effect of the nano/microscale structure of biomaterial scaffolds on bone regeneration10.1038/s41368-020-0073-y ↗
6. Bone substitutes: a review of their characteristics, clinical use, and perspectives for large bone defects management10.1177/2041731418776819 ↗
7. It takes two to tango: coupling of angiogenesis and osteogenesis for bone regeneration10.3389/fbioe.2017.00068 ↗
8. Bringing new life to damaged bone: the importance of angiogenesis in bone repair and regeneration10.1016/j.bone.2014.09.017 ↗
9. Role of angiocrine signals in bone development, homeostasis and disease
10. Osteogenesis and angiogenesis: the potential for engineering bone10.22203/eCM.v015a08 ↗
11. Review on the strategies to improve the mechanical strength of highly porous bone bioceramic scaffolds10.1016/j.jeurceramsoc.2023.09.003 ↗
12. Calcium phosphate bone graft substitutes: failures and hopes10.1016/j.jeurceramsoc.2012.02.028 ↗
13. Magnesium ion stimulation of bone marrow stromal cells enhances osteogenic activity, simulating the effect of magnesium alloy degradation10.1016/j.actbio.2014.02.002 ↗
14. Magnesium promotes bone formation and angiogenesis by enhancing MC3T3-E1 secretion of PDGF-BB10.1016/j.bbrc.2020.05.113 ↗
15. The roles of ions on bone regeneration10.1016/j.drudis.2018.01.049 ↗
16. In vitro osteogenesis by intracellular uptake of strontium containing bioactive glass nanoparticles10.1016/j.actbio.2017.11.008 ↗
17. Human mesenchymal stem cells differentiate into an osteogenic lineage in presence of strontium containing bioactive glass nanoparticles10.1016/j.actbio.2019.03.038 ↗
18. The effect of silicate ions on proliferation, osteogenic differentiation and cell signalling pathways (WNT and SHH) of bone marrow stromal cells10.1039/C2BM00108J ↗
19. Proliferation and osteoblastic differentiation of human bone marrow-derived stromal cells on akermanite-bioactive ceramics10.1016/j.biomaterials.2006.07.027 ↗
20. Understanding of dopant-induced osteogenesis and angiogenesis in calcium phosphate ceramics10.1016/j.tibtech.2013.06.005 ↗
21. Effects of MgO, ZnO, SrO, and SiO 2 in tricalcium phosphate scaffolds on in vitro gene expression and in vivo osteogenesis10.1016/j.msec.2018.10.073 ↗
22. Ionic substituted hydroxyapatite for bone regeneration applications: a review
23. Effect of silicon level on rate, quality and progression of bone healing within silicate-substituted porous hydroxyapatite scaffolds10.1016/j.biomaterials.2006.05.039 ↗
24. Sr,Mg co-doping of calcium hydroxyapatite: hydrothermal synthesis, processing, characterization and possible application as dentin substitutes10.1016/j.ceramint.2021.12.335 ↗
25. Mg2+, Sr2+, Ag+, and Cu2+ co-doped β-tricalcium phosphate: improved thermal stability and mechanical and biological properties10.1111/jace.19093 ↗
26. β-TCP/S53P4 scaffolds obtained by gel casting: synthesis, properties, and biomedical applications10.3390/bioengineering10050597 ↗
27. Bioactive calcium phosphate-based composites for bone regeneration10.3390/jcs5090227 ↗
28. Phase formation and evolution in the silicon substituted tricalcium phosphate/apatite system10.1016/j.biomaterials.2004.09.005 ↗
29. Mg/Cu co-substituted hydroxyapatite – biocompatibility, mechanical properties and antimicrobial activity10.1016/j.ceramint.2019.07.219 ↗
30. Hydroxyapatite-based dental inserts: microstructure, mechanical properties, bonding efficiency and fracture resistance of molars with occlusal restorations
31. Processing of gelatine coated composite scaffolds based on magnesium and strontium doped hydroxyapatite and yttria-stabilized zirconium oxide10.2298/SOS220723019G ↗
32. Mesoporous bioactive glasses for regenerative medicine
33. Mesoporous bioactive nanoparticles for bone tissue applications10.3390/ijms24043249 ↗
34. Achievements in mesoporous bioactive glasses for biomedical applications10.3390/pharmaceutics14122636 ↗
35. Mesoporous bioactive glasses for bone healing and biomolecules delivery10.1016/j.msec.2019.110180 ↗
36. Mesoporous bioactive glass for synergistic therapy of tumor and regeneration of bone tissue
37. Unraveling the role of calcium in the osteogenic behavior of mesoporous bioactive glass nanoparticles10.1016/j.actbio.2025.04.005 ↗
38. Multifunctional Sr,Mg-doped mesoporous bioactive glass nanoparticles for simultaneous bone regeneration and drug delivery10.3390/ijms25158066 ↗
39. Preparation and characterization of highly porous ceramic-based nanocomposite scaffolds with improved mechanical properties using the liquid phase-assisted sintering method
40. Biological properties of calcium phosphate bioactive glass composite bone substitutes: current experimental evidence10.3390/ijms20020305 ↗
41. Mg and/or Sr-doped tricalcium phosphate/bioactive glass composites: synthesis, microstructure and biological responsiveness10.1016/j.msec.2014.05.047 ↗
42. Hydroxyapatite and tricalcium phosphate composites with bioactive glass as second phase: State of the art and current applications10.1002/jbm.a.35619 ↗
43. 3D-printed β-TCP/S53P4 bioactive glass scaffolds coated with tea tree oil: coating optimization, in vitro bioactivity and antibacterial properties10.1002/jbm.b.35198 ↗
44. Validation of a novel perfusion bioreactor system in cancer research10.2298/HEMIND200329015S ↗
45. Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: enhanced hydroxyapatite formation under biomimetic conditions10.1002/jbm.b.34856 ↗
46. Multiparameter analysis of human bone marrow stromal cells identifies distinct immunomodulatory and differentiation-competent subtypes10.1016/j.stemcr.2015.05.005 ↗
47. R. Core Team, R: A language and environment for statistical computing. R Foundation for Statistical Computing, (2023). 〈https://www.R-project.org〉.
48. team · 2023
49. Welcome to the Tidyverse10.21105/joss.01686 ↗
50. Foam replica method in the manufacturing of bioactive glass scaffolds: out-of-date technology or still underexploited potential?10.3390/ma14112795 ↗
51. Sintering of magnesium-strontium doped hydroxyapatite nanocrystals: towards the production of 3D biomimetic bone scaffolds10.1002/jbm.a.36843 ↗
52. Sr and Mg doped Bi-phasic calcium phosphate macroporous bone graft substitutes fabricated by robocasting: a structural and cytocompatibility assessment10.3390/jfb13030123 ↗
53. Si, Zn-modified tricalcium phosphates: a phase composition and crystal structure study
54. Effect of microporosity on scaffolds for bone tissue engineering10.1093/rb/rby001 ↗
55. The osteogenic response of mesenchymal stromal cells to strontium-substituted bioactive glasses10.1002/term.2003 ↗
56. The influence of strontium release rate from bioactive phosphate glasses on osteogenic differentiation of human mesenchymal stem cells10.1016/j.jeurceramsoc.2017.08.005 ↗
57. The role of magnesium ions in bone regeneration involves the canonical Wnt signaling pathway10.1016/j.actbio.2019.06.001 ↗
58. Osseointegration of osteoporotic bone implants: role of stem cells, Silica and Strontium - a concise review
59. Magnesium basics
60. Magnesium modification of a calcium phosphate cement alters bone marrow stromal cell behavior via an integrin-mediated mechanism10.1016/j.biomaterials.2015.02.097 ↗
61. Magnesium modification up-regulates the bioactivity of bone morphogenetic protein-2 upon calcium phosphate cement via enhanced BMP receptor recognition and Smad signaling pathway10.1016/j.colsurfb.2016.04.045 ↗
62. Interaction of Sr-doped hydroxy apatite nanocrystals with osteoclast and osteoblast-like cells10.1002/jbm.a.31975 ↗
63. Strontium-substituted hydroxyapatite coatings synthesized by pulsed-laser deposition: in vitro osteoblast and osteoclast response10.1016/j.actbio.2008.05.005 ↗
64. Strontium promotes the proliferation and osteogenic differentiation of human placental decidual basalis- and bone marrow-derived MSCs in a dose-dependent manner10.1155/2019/4242178 ↗
65. 3D printed scaffolds of calcium silicate-doped β-TCP synergize with co-cultured endothelial and stromal cells to promote vascularization and bone formation
66. Optimizing silicon doping levels for enhanced osteogenic and angiogenic properties of 3D-printed biphasic calcium phosphate scaffolds: an in vitro screening and in vivo validation study
67. A novel calcium phosphate-based ceramic scaffolds with unexpected high osteogenic activity by strontium doping
68. Adjusting physicochemical and cytological properties of biphasic calcium phosphate by magnesium substitution: an in vitro study10.1016/j.ceramint.2023.01.148 ↗
69. Strontium-incorporated bioceramic scaffolds for enhanced osteoporosis bone regeneration10.1038/s41413-022-00224-x ↗
70. Biocompatible magnesium-doped biphasic calcium phosphate for bone regeneration10.1002/jbm.b.34802 ↗
71. Binary doping of strontium-magnesium to bioactive glasses to enhance antibacterial and osteogenic effects
72. Biological evaluation of porous nanocomposite scaffolds based on strontium substituted β-TCP and bioactive glass: an in vitro and in vivo study10.1016/j.msec.2019.110071 ↗
73. Magnesium (Mg2 +), strontium (Sr2 +), and zinc (Zn2 +) co-substituted bone cements based on nano-hydroxyapatite/monetite for bone regeneration10.1007/s12011-022-03382-5 ↗
74. Vascularization in bone tissue engineering constructs10.1007/s10439-015-1253-3 ↗
75. Enhancing mechanical property, osteogenesis and angiogenesis of 3D-plotted β-tricalcium phosphate bioceramic scaffolds incorporated with magnesium silicate: in vitro and in vivo study10.1016/j.jeurceramsoc.2023.12.048 ↗