Poly (Bromophenol Blue)-Gold Nanoparticle Composite: An Efficient Electrochemical Sensing Platform for Uric Acid

A simple voltammetric sensor has been developed for direct determination of important biomarker uric acid (UA) based on poly bromophenol blue - gold nanoparticles composite modified glassy carbon electrode. Scanning electron microscopy, atomic force microscopy and various electrochemical techniques were used for characterization of modified electrode surface. The electro-oxidation of UA in 0.1 M NaOH was excellently catalyzed by modified electrode. The anodic peak corresponding to the oxidation of UA was observed at a potential of −105 mV by Square wave voltammetry (SWV). The oxidation peak current was increased linearly on increasing the concentration of UA in the range 1.0 × 10 −3 to 2.0 × 10 −5 M (R 2 = 0.994) with a limit of detection 2.2 × 10 −6 M. The proposed method was effectively applied to determine Uric acid in artificial blood serum and urine samples. The developed sensor would be a potential alternative tool for UA determination in biological samples.