Non-Enzymatic Electrochemical Sensors for Accurate and Accessible Uric Acid Detection

Gout arthritis, a painful condition characterized by the accumulation of uric acid (UA) crystals in the joints, necessitates the development of efficient and accessible diagnostic methods. In this study, we present the design and fabrication of non-enzymatic electrochemical sensors based on molecularly imprinted conducting polymer nanostructures for the selective detection and monitoring of UA. To overcome the limitations of current point-of-care (POC) diagnostics, we developed a disposable voltammetric sensor capable of selectively and accurately analyzing UA concentrations at room temperature and pH 7.4. The sensor utilizes a selective polythiophene layer, deposited on disposable screen-printed electrodes, for the reversible binding and recognition of UA. Extensive electrochemical measurements employing electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and differential pulse voltammetry (DPV) demonstrate excellent UA sensing performance in the wider concentration range of 1–500 μM using a standard redox solution with 2.91 μA cm⁻²·μM⁻¹ sensitivity, 354 nM detection limit, and substantial selectivity over common interferents. The sensor is capable of analyzing complex mixtures such as human saliva with 90.4% recovery of the spiked UA concentration. This highly sensitive and cost-efficient electrochemical sensor offers great promise for POC testing, disease monitoring, and predicting treatment outcomes related to gout arthritis.