Preparation and characterization of hydroxyapatite incorporated silica aerogel and its effect on normal human dermal fibroblast cells
Source abstract
Abstract
The development of biomedical materials is increasing nowadays due to the increasing demands in medical area and various manmade advanced materials have been explored. In this study, the hydroxyapatite (HA) which is widely used biomaterial has been incorporated in silica aerogel (SA) through sol-gel ambient pressure drying (APD) technique using amorphous silica from rice husk ash. The HA incorporated SA (HAESA) samples with different weight ratio of HA/SiO2 (0.05, 0.1, 0.5, 1.0 and 1.3) were characterized and studied for their in vitro biocompatibility against normal human fibroblast cells. Based on the characterization results from Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscope, energy dispersive X-ray analysis, transmission electron microscope and thermogravimetric analysis, the HAESA samples with lower weight ratio of HA/SiO2 (0.05, 0.1 and 0.5) were silica-rich materials compared to that of higher weight ratio (1.0 and 1.3) which were silica-deficient materials. This resulted in different effects towards in vitro cell viability where the silica-rich HAESA samples have higher biocompatibility compared to that of silica-deficient materials based on trypan blue and cell viability assays. The optimum weight ratio of HA/SiO2 was for HAESA-0.5 (weight ratio of 0.5) in which it has the characteristics of HA and SA, and this eventually resulted in the highest cell viability which is up to 180% after 48 h exposure. Therefore, it can be concluded that the right amount of HA incorporated in the SA network can be performed by using sol-gel APD technique and resulted in high biocompatibility which is suitable to be used as alternative biomaterial for soft tissue application.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Aerogels and thermal insulation · Bone Tissue Engineering Materials · Silicone and Siloxane Chemistry
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (45)
Show source-provided references
1. Huggins RJ, Mendelson BC (2017) Biologic behavior of hydroxyapatite used in facial augmentation. Aesthetic Plast Surg 41(1):179–18410.1007/s00266-016-0707-9 ↗
2. Kawai K, Larson BJ, Ishise H, Carre AL, Nishimoto S, Longaker M, Lorenz HP (2011) Calcium-based nanoparticles accelerate skin wound healing. PLoS One 6(11):e27106–e2711910.1371/journal.pone.0027106 ↗
3. Muslim YR, Knowles J, Howlett J (2018) Mechanical properties of glass reinforced hydroxyapatite. Ann Dent Univ Malaya 12(1):31–36
4. Porter AE, Patel N, Skepper JN, Best SM, Bonfield W (2003) Comparison of in vivo dissolution processes in hydroxyapatite and silicon-substituted hydroxyapatite bioceramics Biomater 24(25):4609–462010.1016/S0142-9612(03)00355-7 ↗
5. Thian ES, Huang J, Best SM, Barber ZH, Brooks RA, Rushton N, Bonfield W (2006) The response of osteoblasts to nanocrystalline silicon-substituted hydroxyapatite thin films. Biomaterials 27(13):2692–269810.1016/j.biomaterials.2005.12.019 ↗
6. Ma G, Liu XY (2009) Hydroxyapatite: hexagonal or monoclinic? Cryst Growth Des 9(7):2991–299410.1021/cg900156w ↗
7. Bohner M (2009) Silicon-substituted calcium phosphates - a critical view. Biomaterials 30(32):6403–640610.1016/j.biomaterials.2009.08.007 ↗
8. Balamurugan A, Rebelo AHS, Lemos AF, Rocha JHG, Ventura JMG, Ferreira JMF (2008) Suitability evaluation of sol-gel derived Si-substituted hydroxyapatite for dental and maxillofacial applications through in vitro osteoblasts response. Dent Mater 24(10):1374–138010.1016/j.dental.2008.02.017 ↗
9. Ravarian R, Moztarzadeh F, Hashjin MS, Rabiee SM, Khoshakhlagh P, Tahriri M (2010) Synthesis, characterization and bioactivity investigation of bioglass/hydroxyapatite composite. Ceram Int 36(1):291–29710.1016/j.ceramint.2009.09.016 ↗
10. Avnir D, Coradin T, Lev O, Livage J (2006) Recent bio-applications of sol–gel materials. J Mater Chem 16(11):1013–103010.1039/B512706H ↗
11. Taherian M, Rojaee R, Fathi M (2014) Effect of different sol-gel synthesis processes on microstructural and morphological characteristics of hydroxyapatite-bioactive glass composite nanopowders. J Adv Ceram 3(3):207–21410.1007/s40145-014-0111-3 ↗
12. Vallet-Regí M, Arcos D (2005) Silicon substituted hydroxyapatites. A method to upgrade calcium phosphate based implants. J Mater Chem 15(15):1509–151610.1039/B414143A ↗
13. Pietak AM, Reid JW, Stott MJ, Sayer M (2007) Silicon substitution in the calcium phosphate bioceramics. Biomaterials 28(28):4023–403210.1016/j.biomaterials.2007.05.003 ↗
14. Qiu ZY, Noh IS, Zhang SM (2013) Silicate-doped hydroxyapatite and its promotive effect on bone mineralization. Front Mater Sci 7(1):40–5010.1007/s11706-013-0193-9 ↗
15. Ciriminna R, Fidalgo A, Pandarus V, Béland F, Ilharco LM, Pagliaro M, Beand F, Ilharco LM, Pagliaro M (2013) The sol-gel route to advanced silica-based materials and recent applications. Chem Rev 113(8):6592–662010.1021/cr300399c ↗
16. Owens GJ, Singh RK, Foroutan F, Alqaysi M, Han CM, Mahapatra C, Kim HW, Knowles JC (2016) Sol-gel based materials for biomedical applications. Prog Mater Sci 77:1–7910.1016/j.pmatsci.2015.12.001 ↗
17. Brinker CJ (1994) In: Bergna HE (ed) The colloid chemistry of silica. Washington, American Chemical Society, p 363–402
18. Brinker CJ, Scherer GW (1990) Sol-gel Science: the physics and chemistry of sol-gel processing. Boston, MA, Academic Press
19. Cai H, Sharma S, Liu WW, Mu W, Liu WW, Zhang X, Deng Y (2014) Aerogel microspheres from natural cellulose nanofibrils and their application as cell culture scaffold. Biomacromolecules 15(7):2540–254710.1021/bm5003976 ↗
20. Sani NS, Malek NANN, Jemon K, Kadir MRA, Hamdan H (2017) Effect of mass concentration on bioactivity and cell viability of calcined silica aerogel synthesized from rice husk ash as silica source. J Sol-Gel Sci Technol 82:120–13210.1007/s10971-016-4266-y ↗
21. He S, Huang D, Bi H, Li Z, Yang H, Cheng X (2015) Synthesis and characterization of silica aerogels dried under ambient pressure bed on water glass. J Non Cryst Solids 410:58–6410.1016/j.jnoncrysol.2014.12.011 ↗
22. Maleki H, Duraes L, Garcia-Gonzalez CA, del Gaudio P, Portugal A, Mahmoudi M (2016) Synthesis and biomedical applications of aerogels: Possibilities and challenges. Adv Colloid Interface Sci 236:1–2710.1016/j.cis.2016.05.011 ↗
23. Stergar J, Maver U (2016) Review of aerogel-based materials in biomedical applications. J Sol-Gel Sci Technol 77(3):738–75210.1007/s10971-016-3968-5 ↗
24. Yusof AM, Nizam NA, Rashid NAA (2010) Hydrothermal conversion of rice husk ash to faujasite-types and NaA-type of zeolites. J Porous Mater 17(1):39–4710.1007/s10934-009-9262-y ↗
25. Sani NS, Malek NANN, Kadir MRA, Hamdan H (2015) Study on the preliminary development of synthesis methods for hydroxyapatite modified silica aerogel. Appl Mech Mater 799–800:493–49910.4028/www.scientific.net/AMM.799-800.493 ↗
26. Hing KA, Revell PA, Smith N, Buckland T (2006) Effect of silicon level on rate, quality and progression of bone healing within silicate-substituted porous hydroxyapatite scaffolds. Biomaterials 27(29):5014–502610.1016/j.biomaterials.2006.05.039 ↗
27. Xian W (2009) A Laboratory Course in Biomaterials. Boca Raton, Florida, CRC Press10.1201/b15832 ↗
28. Avelar-Freitas BA, Almeida VG, Pinto MCX, Mourão FAG, Massensini AR, Martins-Filho OA, Rocha-Vieira E, Brito-Melo GEA (2014) Trypan blue exclusion assay by flow cytometry. Braz J Med Biol Res 47(4):307–31510.1590/1414-431X20143437 ↗
29. Dunn B, Miller JM, Dave BC, Valentine JS, Zink JI (1998) Strategies for encapsulating biomolecules in sol-gel matrices. Acta Mater 46(3):737–74110.1016/S1359-6454(97)00254-1 ↗
30. Shewale PM, Rao AV, Rao AP (2008) Effect of different trimethyl silylating agents on the hydrophobic and physical properties of silica aerogels. Appl Surf Sci 254(21):6902–690710.1016/j.apsusc.2008.04.109 ↗
31. Aminian A, Solati-Hashjin M, Samadikuchaksaraei A, Bakhshi F, Gorjipour F, Farzadi A, Moztarzadeh F, Schmücker M (2011) Synthesis of silicon-substituted hydroxyapatite by a hydrothermal method with two different phosphorous sources. Ceram Int 37(4):1219–122910.1016/j.ceramint.2010.11.044 ↗
32. Ananth KP, Shanmugam S, Joseb SP, Nathanael AJ, Oh TH, Mangalaraj D, Ballamurugan AM (2015) Structural and chemical analysis of silica-doped β-TCP ceramic coatings on surgical grade 316L SS for possible biomedical application. J Asian Ceram Soc 3(3):317–32410.1016/j.jascer.2015.06.004 ↗
33. Matesanz MC, Linares J, Oñaderra M, Feito MJ, Martínez-Vázquez FJ, Sánchez-Salcedo S, Arcos D, Portolés MT, Vallet-Regí M (2015) Response of osteoblasts and preosteoblasts to calcium deficient and Si substituted hydroxyapatites treated at different temperatures. Colloid Surf B 133:304–31310.1016/j.colsurfb.2015.06.014 ↗
34. Wawrzyniak P, Rogacki G, Pruba J, Bartczak Z (2001) Effective diffusion coefficient in the low temperature process of silica aerogel production. J Non Cryst Solids 285(1–3):50–5610.1016/S0022-3093(01)00431-8 ↗
35. Sani S, Mohd Muhid MN, Hamdan H (2011) Design, synthesis and activity study of tyrosinase encapsulated silica aerogel (TESA) biosensor for phenol removal in aqueous solution. J Sol-Gel Sci Technol 59(1):7–1810.1007/s10971-011-2454-3 ↗
36. Tien W-B, Chen M-T, Yao P-C (2012) Effects of pH and temperature on microstructure and morphology of hydroxyapatite/collagen composites synthesized in vitro. Mater Sci Eng C 32:2096–210210.1016/j.msec.2012.05.046 ↗
37. Shen J, Jin B, Hu Y, Jiang Q (2015) An effective route to the synthesis of carbonated apatite crystals with controllable morphologies and their growth mechanism Cryst Eng Comm 17(29):5422–543010.1039/C5CE00812C ↗
38. Lazić S (1995) Microcrystalline hydroxyapatite formation from alkaline solutions. J Cryst Growth 147(1–2):147–15410.1016/0022-0248(94)00587-7 ↗
39. Gómez-Morales J, Iafisco M, Delgado-López JM, Sarda S, Drouet C (2013) Progress on the preparation of nanocrystalline apatites and surface characterization: overview of fundamental and applied aspects. Prog Cryst Growth Charact Mater 59(1):1–4610.1016/j.pcrysgrow.2012.11.001 ↗
40. Bhagat SD, Kim YH, Moon MJ, Ahn YS, Yeo JG (2007) A cost-effective and fast synthesis of nanoporous SiO2 aerogel powders using water-glass via ambient pressure drying route. Solid State Sci 9(7):628–63510.1016/j.solidstatesciences.2007.04.020 ↗
41. Suchanek WL, Shuk P, Byrappa K, Riman RE, TenHuisen KS, Janas VF (2002) Mechanochemical-hydrothermal synthesis of carbonated apatite powders at room temperature. Biomaterials 23(3):699–71010.1016/S0142-9612(01)00158-2 ↗
42. Alqap ASF, Adzila S, Sopyan I, Hamdi M, Ramesh S (2011) Thermal analysis on hydroxyapatite synthesis through mechanochemical method. In: 5th Kuala Lumpur International Conference on Biomedical Engineering 2011, 108–111, Springer, Berlin, Heidelberg10.1007/978-3-642-21729-6_30 ↗
43. Cuce E, Cuce PM, Wood CJ, Riffat SB (2014) Toward aerogel based thermal superinsulation in buildings: a comprehensive review. Renew Sustain Energy Rev 34:273–29910.1016/j.rser.2014.03.017 ↗
44. Tran SL, Puhar A, Ngo-Camus M, Ramarao N (2011) Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus. PLoS One 6(9):2–6
45. Arcangeli G, Cupelli V, Giuliano G (2001) Effects of silica on human lung fibroblast in culture. Sci Total Environ 270(1–3):135–13910.1016/S0048-9697(00)00781-6 ↗