Design, synthesis and activity study of tyrosinase encapsulated silica aerogel (TESA) biosensor for phenol removal in aqueous solution
Source abstract
Abstract
Tyrosinase encapsulated silica aerogel (TESA) was synthesized via an alcohol-free colloidal sol–gel route at room temperature and at neutral pH. Characterization on TESA indicated that 98% of enzyme was effectively loaded and located inside the aerogel network. TESA without solvent extraction showed higher tyrosinase activity than TESA extracted by amyl acetate/acetone (v/v:1/1). Stability of tyrosinase in TESA was enhanced towards extreme temperature, acidic and basic conditions. Optimization study indicates that 500 U enzyme/g silica aerogel; aged for 2 days, showed superior performance in the oxidation of catechol. The activity of TESA was remarkably enhanced; which was active at a wider temperature (up to 80 °C) and pH range (4–9). In contrast, free tyrosinase was totally inactive at these pH values and temperature >55 °C. TESA successfully removed about 90% of phenol in aqueous solution after 3 h of contact time with excellent reusability.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Aerogels and thermal insulation · melanin and skin pigmentation
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (39)
Show source-provided references
1. Girelli AM, Mattei E, Messina A (2006) Anal Chim Acta 580:27110.1016/j.aca.2006.07.088 ↗
2. Aitken MD (1993) Chem Eng J 52:4910.1016/0300-9467(93)80042-M ↗
3. Britto JM, Rabelo D, Rangel M (2008) Catal Today 133:58210.1016/j.cattod.2007.12.112 ↗
4. Sathishkumar M, Vijayaraghavan K, Binupriya AR, Stephan AM, Choi JG, Yun SE (2008) J Colloid Interface Sci 320:2210.1016/j.jcis.2007.12.011 ↗
5. Adak A, Pal A, Bandyopadhyay M (2006) Colloids Surf. A 277:63
6. González PS, Capozucca CE, Tigier HA, Milrad SR, Agostini E (2006) Enzyme Microb Technol 39:64710.1016/j.enzmictec.2005.11.014 ↗
7. Karam J, Nicell JA (1997) J Chem Tech Biotechnol 69:14110.1002/(SICI)1097-4660(199706)69:2<141::AID-JCTB694>3.0.CO;2-U ↗
8. Siddique MH, St Pierre CC, Biswas N, Bewtra JK, Taylor KE (1993) Water Res 27:88310.1016/0043-1354(93)90153-9 ↗
9. Atlow SC, Bonadonna-Aparo L, Klibanov AM (1984) Biotechnol Bioeng 26:59910.1002/bit.260260607 ↗
10. Messing RA (1975) Immobilized enzymes for industrial reactors. Academic Press, New York
11. Monsan P, Combes D (1998) Methods Enzymol 137:58410.1016/0076-6879(88)37055-2 ↗
12. Bergogne L, Fennouh S (2000) Mol Cryst Liq Cryst 354:66710.1080/10587250008023605 ↗
13. Messing RA (1975) Immobilized enzymes for industrial reactors. Academic Press Inc, New York
14. Fessner WD (1999) Biocatalysis: from discovery to application. Springer, Berlin10.1007/3-540-68116-7 ↗
15. Bhatia RB, Brinker CJ, Gupta AK, Singh AK (2000) Chem Mater 122:434
16. Maury S, Buisson P, Perrard A, Pierre AC (2005) J Mol Catal B Enzymatic 32:19310.1016/j.molcatb.2004.12.006 ↗
17. Hench L, West JK (1990) ChemRev 90:33
18. Brinker CJ, Scherer GW (1990) Sol-gel science: the physics and chemistry of sol-gel processing. Academic Press, Boston
19. Hamdan H (2011) United States Patent. US7,897,648 B2
20. Stolarski M, Walendziewski J, Steininger M, Pniak B (1999) Appl Catal A 177:13910.1016/S0926-860X(98)00296-8 ↗
21. Prakash SS, Brinker CJ, Hurd AJ, Rao SM (1995) Nature 374:43910.1038/374439a0 ↗
22. Schwertfeger F, Frank D, Schmidt M (1998) J Non Cryst Solids 225:2410.1016/S0022-3093(98)00102-1 ↗
23. Shi F, Wang L, Lui J (2006) Mater Lett 60:371810.1016/j.matlet.2006.03.095 ↗
24. Rao AP, Rao AV, Pajonk GM (2005) J Sol Gel Sci Technol 36:28510.1007/s10971-005-4662-1 ↗
25. Bhagat SD, Kim YH, Ahn YS, Yeo JG (2007) Appl Surf Sci 253:323110.1016/j.apsusc.2006.07.016 ↗
26. Gill I, Ballesteros A (2000) Trends Biotechnol 18:28210.1016/S0167-7799(00)01457-8 ↗
27. Dunn B, Miller JM, Dave BC (1998) Acta Mater 46:73710.1016/S1359-6454(97)00254-1 ↗
28. Liu DM, Chen IW (1999) Acta Mater 47:453510.1016/S1359-6454(99)00335-3 ↗
29. Seetharam GB, Saville BA (2003) Water Res 37:43610.1016/S0043-1354(02)00290-7 ↗
30. Simms BL (1991) US patent 5017354
31. Robson H (2001) Verified synthesis of zeolitic materials. Elsevier, Amsterdam
32. Hua DW, Anderson J, Gregorio J, Smith DM, Beaucage G (1995) J Non Cryst Solids 186:14210.1016/0022-3093(95)00044-5 ↗
33. Vansant EF, Van Der Voort P, Vrancken KC (1995) Characterization and chemical modification of the silica surface. Elsevier Science, The Netherlands
34. Eberhardt AM, Pedroni V, Volpe M, Ferreira ML (2004) Appl Catal B 47(3):15310.1016/j.apcatb.2003.08.007 ↗
35. Tyagi R, Gupta MN (1998) Biotechnol Tech 12(7):56910.1023/A:1008820018265 ↗
36. Warrington JC, Saville BA (1999) Biotechnol Bioeng 65(3):32510.1002/(SICI)1097-0290(19991105)65:3<325::AID-BIT10>3.0.CO;2-Q ↗
37. Sun WQ, Payne GF, Moas MSGL, Wallance KK (1992) Biotechnol Progr 8:17910.1021/bp00015a002 ↗
38. Wada S, Ichiwa H, Tatsumi K (1992) Water Sci Technol 26:205710.1021/es00035a903 ↗
39. Ensuncho L, Alvarez-Cuenca M, Legge RL (2005) Bioprocess Biosyst Eng 27:18510.1007/s00449-005-0400-x ↗