Injectable gel from squid pen chitosan for bone tissue engineering applications
Source abstract
Abstract
The aim of this study was to evaluate the potential of squid pen chitosan for developing injectable gels for bone tissue engineering applications. Gel mixtures made of glycerol phosphate mixed with crab (RC) or squid pen (RS) chitosan (2 % w/v) at four different concentrations (0, 30, 50 and 70 %) of calcium phosphate compounds (CaP, hydroxyapatite and β-tricalcium phosphate, HA/β-TCP) were investigated for their biocompatibility and mechanical properties. The proposed gel rapidly settled ( 2000 %), compressive modulus (up to 26 kPa) and better cell (Saos-2) compatibility compared to the RC chitosan. This study showed that RS chitosan is a promising alternative to commercially available crab/shrimp chitosan for producing injectable gels for tissue engineering applications.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Electrospun Nanofibers in Biomedical Applications · biodegradable polymer synthesis and properties · Nanocomposite Films for Food Packaging
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (57)
Show source-provided references
1. Huang Z, Tian J, Yu B, Xu Y, Feng Q (2009) A bone-like nano-hydroxyapatite/collagen loaded injectable scaffold. Biomed Mater 4:05500510.1088/1748-6041/4/5/055005 ↗
2. Soundrapandian C, Datta S, Kundu B, Basu D, Sa B (2010) Porous bioactive glass scaffolds for local drug delivery in osteomyelitis: development and in vitro characterization. AAPS PharmSciTech 11:1675–168310.1208/s12249-010-9550-5 ↗
3. Mishra D, Bhunia B, Banerjee I, Datta P, Dhara S, Maiti TK (2011) Enzymatically crosslinked carboxymethyl–chitosan/gelatin/nano-hydroxyapatite injectable gels for in situ bone tissue engineering application. Mater Sci Eng, C 31:1295–130410.1016/j.msec.2011.04.007 ↗
4. Bi L, Cheng W, Fan H, Pei G (2010) Reconstruction of goat tibial defects using an injectable tricalcium phosphate/chitosan in combination with autologous platelet-rich plasma. Biomaterials 31:3201–321110.1016/j.biomaterials.2010.01.038 ↗
5. García Cruz DM, Escobar Ivirico JL, Gomes MM, Gómez Ribelles JL, Sánchez MS, Reis RL et al (2008) Chitosan microparticles as injectable scaffolds for tissue engineering. J Tissue Eng Regen Med 2:378–38010.1002/term.106 ↗
6. Huang Z, Feng Q, Yu B, Li S (2011) Biomimetic properties of an injectable chitosan/nano-hydroxyapatite/collagen composite. Mater Sci Eng, C 31:683–68710.1016/j.msec.2010.12.014 ↗
7. Shen D, Wang X, Zhang L, Zhao X, Li J, Cheng K et al (2011) The amelioration of cardiac dysfunction after myocardial infarction by the injection of keratin biomaterials derived from human hair. Biomaterials 32:9290–929910.1016/j.biomaterials.2011.08.057 ↗
8. Eeckman F, Moës AJ, Amighi K (2004) Poly(N-isopropylacrylamide) copolymers for constant temperature controlled drug delivery. Int J Pharm 273:109–11910.1016/j.ijpharm.2003.12.013 ↗
9. Stile RA, Chung E, Burghardt WR, Healy KE (2004) Poly(N-isopropylacrylamide)-based semi-interpenetrating polymer networks for tissue engineering applications. Effects of linear poly(acrylic acid) chains on rheology. J Biomater Sci Polym Ed 15:865–87810.1163/1568562041271129 ↗
10. Thein-Han WW, Misra RDK (2009) Biomimetic chitosan–nanohydroxyapatite composite scaffolds for bone tissue engineering. Acta Biomater 5:1182–119710.1016/j.actbio.2008.11.025 ↗
11. Qiu Y (2008) Chitosan derivatives for tissue engineering. Clemson University, Clemson
12. Jiang CJ, Xu MQ (2006) Kinetics of heterogeneous deacetylation of β-chitin. Chem Eng Technol 29:511–51610.1002/ceat.200500293 ↗
13. Kurita K (2001) Controlled functionalization of the polysaccharide chitin. Prog Polym Sci 26:1921–197110.1016/S0079-6700(01)00007-7 ↗
14. Kurita K, Tomita K, Tada T, Ishii S, Nishimura S-I, Shimoda K (1993) Squid chitin as a potential alternative chitin source: deacetylation behavior and characteristic properties. J Polym Sci, Part A: Polym Chem 31:485–49110.1002/pola.1993.080310220 ↗
15. Lima IS, Airoldi C (2004) A thermodynamic investigation on chitosan–divalent cation interactions. Thermochim Acta 421:133–13910.1016/j.tca.2004.03.012 ↗
16. Susana Cortizo M, Berghoff CF, Alessandrini JL (2008) Characterization of chitin from Illex argentinus squid pen. Carbohydr Polym 74:10–1510.1016/j.carbpol.2008.01.004 ↗
17. Rinaudo M (2006) Chitin and chitosan: properties and applications. Prog Polym Sci 31:603–63210.1016/j.progpolymsci.2006.06.001 ↗
18. Chandumpai A, Singhpibulporn N, Faroongsarng D, Sornprasit P (2004) Preparation and physico-chemical characterization of chitin and chitosan from the pens of the squid species, Loligo lessoniana and Loligo formosana. Carbohydr Polym 58:467–47410.1016/j.carbpol.2004.08.015 ↗
19. Liu H, Li H, Cheng W, Yang Y, Zhu M, Zhou C (2006) Novel injectable calcium phosphate/chitosan composites for bone substitute materials. Acta Biomater 2:557–56510.1016/j.actbio.2006.03.007 ↗
20. Cho J, Heuzey M-C, Bégin A, Carreau PJ (2005) Physical gelation of chitosan in the presence of β-glycerophosphate: the effect of temperature. Biomacromolecules 6:3267–327510.1021/bm050313s ↗
21. Zhou HY, Jiang LJ, Cao PP, Li JB, Chen XG (2015) Glycerophosphate-based chitosan thermosensitive hydrogels and their biomedical applications. Carbohydr Polym 117:524–53610.1016/j.carbpol.2014.09.094 ↗
22. Shavandi A, Bekhit AE-D, Ali A, Sun Z (2015) A novel squid pen chitosan/hydroxyapatite/β-tricalcium phosphate composite for bone tissue engineering. Mater Sci Eng C 55:373–38310.1016/j.msec.2015.05.029 ↗
23. Shavandi A, Bekhit AE-D, Ali A, Sun Z, Ratnayake JT (2015) Microwave-assisted synthesis of high purity β-tricalcium phosphate crystalline powder from the waste of Green mussel shells (Perna canaliculus). Powder Technol 273:33–3910.1016/j.powtec.2014.12.029 ↗
24. Shavandi A, Bekhit AE-D, Ali A, Sun Z (2015) Synthesis of nano-hydroxyapatite (nHA) from waste mussel shells using a rapid microwave method. Mater Chem Phys 149–150:607–61610.1016/j.matchemphys.2014.11.016 ↗
25. Chaussard G, Domard A (2004) New aspects of the extraction of chitin from squid pens. Biomacromolecules 5:559–56410.1021/bm034401t ↗
26. Youn DK, No HK, Prinyawiwatkul W (2013) Preparation and characteristics of squid pen β-chitin prepared under optimal deproteinisation and demineralisation condition. Int J Food Sci Technol 48:571–57710.1111/ijfs.12001 ↗
27. Xu J, McCarthy SP, Gross RA, Kaplan DL (1996) Chitosan film acylation and effects on biodegradability. Macromolecules 29:3436–344010.1021/ma951638b ↗
28. Yen M-T, Yang J-H, Mau J-L (2009) Physicochemical characterization of chitin and chitosan from crab shells. Carbohydr Polym 75:15–2110.1016/j.carbpol.2008.06.006 ↗
29. Yasmeen S, Lo MK, Bajracharya S, Roldo M (2014) Injectable scaffolds for bone regeneration. Langmuir 30:12977–1298510.1021/la503057w ↗
30. Ahmadi R, de Bruijn JD (2008) Biocompatibility and gelation of chitosan–glycerol phosphate hydrogels. J Biomed Mater Res, Part A 86:824–83210.1002/jbm.a.31676 ↗
31. Tan H, Chu CR, Payne KA, Marra KG (2009) Injectable in situ forming biodegradable chitosan–hyaluronic acid based hydrogels for cartilage tissue engineering. Biomaterials 30:2499–250610.1016/j.biomaterials.2008.12.080 ↗
32. Meng D, Dong L, Wen Y, Xie Q (2015) Effects of adding resorbable chitosan microspheres to calcium phosphate cements for bone regeneration. Mater Sci Eng C Mater Biol Appl 47:266–27210.1016/j.msec.2014.11.049 ↗
33. Zhang X, Zhu L, Lv H, Cao Y, Liu Y, Xu Y et al (2012) Repair of rabbit femoral condyle bone defects with injectable nanohydroxyapatite/chitosan composites. J Mater Sci Mater Med 23:1941–194910.1007/s10856-012-4662-y ↗
34. Li F, Liu Y, Ding Y, Xie Q (2014) A new injectable in situ forming hydroxyapatite and thermosensitive chitosan gel promoted by Na2CO3. Soft Matter 10:2292–230310.1039/C3SM52508B ↗
35. Wang D, Zhang Y, Hong Z (2014) Novel fast-setting chitosan/β-dicalcium silicate bone cements with high compressive strength and bioactivity. Ceram Int 40:9799–980810.1016/j.ceramint.2014.02.069 ↗
36. Fan M, Ma Y, Mao J, Zhang Z, Tan H (2015) Cytocompatible in situ forming chitosan/hyaluronan hydrogels via a metal-free click chemistry for soft tissue engineering. Acta Biomater 20:60–6810.1016/j.actbio.2015.03.033 ↗
37. Biazar E, HeidariKeshel S, Tavirani MR, Jahandideh R (2015) Bone reconstruction in rat calvarial defects by chitosan/hydroxyapatite nanoparticles scaffold loaded with unrestricted somatic stem cells. Artif Cells Nanomed Biotechnol 43:112–11610.3109/21691401.2013.848874 ↗
38. Shirosaki Y, Hirai M, Hayakawa S, Fujii E, Lopes MA, Santos JD et al (2015) Preparation and in vitro cytocompatibility of chitosan–siloxane hybrid hydrogels. J Biomed Mater Res, Part A 103:289–29910.1002/jbm.a.35171 ↗
39. Fatimi A, Tassin JF, Turczyn R, Axelos MA, Weiss P (2009) Gelation studies of a cellulose-based biohydrogel: the influence of pH, temperature and sterilization. Acta Biomater 5:3423–343210.1016/j.actbio.2009.05.030 ↗
40. Ganji F, Abdekhodaie MJ, Ramazani SAA (2007) Gelation time and degradation rate of chitosan-based injectable hydrogel. J Sol-Gel Sci Technol 42:47–5310.1007/s10971-006-9007-1 ↗
41. Kumar GS, Thamizhavel A, Girija EK (2012) Microwave conversion of eggshells into flower-like hydroxyapatite nanostructure for biomedical applications. Mater Lett 76:198–20010.1016/j.matlet.2012.02.106 ↗
42. Dewavrin J-Y, Hamzavi N, Shim VPW, Raghunath M (2014) Tuning the architecture of three-dimensional collagen hydrogels by physiological macromolecular crowding. Acta Biomater 10:4351–435910.1016/j.actbio.2014.06.006 ↗
43. Moreau JL, Xu HHK (2009) Mesenchymal stem cell proliferation and differentiation on an injectable calcium phosphate–chitosan composite scaffold. Biomaterials 30:2675–268210.1016/j.biomaterials.2009.01.022 ↗
44. Ta HT, Dass CR, Dunstan DE (2008) Injectable chitosan hydrogels for localised cancer therapy. J Controll Release 126:205–21610.1016/j.jconrel.2007.11.018 ↗
45. Goldberg RN, Kishore N, Lennen RM (2002) Thermodynamic quantities for the ionization reactions of buffers. J Phys Chem Ref Data 31:231–37010.1063/1.1416902 ↗
46. Burckbuchler V, Mekhloufi G, Giteau AP, Grossiord JL, Huille S, Agnely F (2010) Rheological and syringeability properties of highly concentrated human polyclonal immunoglobulin solutions. Eur J Pharm Biopharm 76:351–35610.1016/j.ejpb.2010.08.002 ↗
47. Chenite A, Chaput C, Wang D, Combes C, Buschmann MD, Hoemann CD et al (2000) Novel injectable neutral solutions of chitosan form biodegradable gels in situ. Biomaterials 21:2155–216110.1016/S0142-9612(00)00116-2 ↗
48. Xu HHK, Simon CG Jr (2005) Fast setting calcium phosphate–chitosan scaffold: mechanical properties and biocompatibility. Biomaterials 26:1337–134810.1016/j.biomaterials.2004.04.043 ↗
49. Wang X, Chen L, Xiang H, Ye J (2007) Influence of anti-washout agents on the rheological properties and injectability of a calcium phosphate cement. J Biomed Mater Res B Appl Biomater 81B:410–41810.1002/jbm.b.30678 ↗
50. Miyamoto Y, Ishikawa K, Takechi M, Yuasa M, Kon M, Nagayama M et al (1996) Non-decay type fast-setting calcium phosphate cement: setting behaviour in calf serum and its tissue response. Biomaterials 17:1429–143510.1016/0142-9612(96)87286-3 ↗
51. Rohindra DR, Nand AV, Khurma JR (2004) Swelling properties of chitosan hydrogels. S Pac J Nat Appl Sci 22:32–3510.1071/SP04005 ↗
52. Barabás R, Czikó M, Dékány I, Bizo L, Bogya E (2013) Comparative study of particle size analysis of hydroxyapatite-based nanomaterials. Chem Pap 67:1414–142310.2478/s11696-013-0409-6 ↗
53. Rudall KM, Kenchington W (1973) The chitin system. Biol Rev 48:597–63310.1111/j.1469-185X.1973.tb01570.x ↗
54. Reys LL, Silva SS, Oliveira JM, Caridade SG, Mano JF, Silva TH et al (2013) Revealing the potential of squid chitosan-based structures for biomedical applications. Biomed Mater 8:1–11
55. Teng SH, Lee EJ, Yoon BH, Shin DS, Kim HE, Oh JS (2009) Chitosan/nanohydroxyapatite composite membranes via dynamic filtration for guided bone regeneration. J Biomed Mater Res, Part A 88:569–58010.1002/jbm.a.31897 ↗
56. Elhendawi H, Felfel RM, Abd El-Hady BM, Reicha FM (2014) Effect of synthesis temperature on the crystallization and growth of in situ prepared nanohydroxyapatite in chitosan matrix. ISRN Biomater 2014:810.1155/2014/897468 ↗
57. Meneghini C, Dalconi MC, Nuzzo S, Mobilio S, Wenk RH (2003) Rietveld refinement on X-ray diffraction patterns of bioapatite in human fetal bones. Biophys J 84:2021–202910.1016/S0006-3495(03)75010-3 ↗