Assessment of antioxidant activity of pure graphene oxide (GO) and composite V2O5/GO using DPPH radical and H2O2 scavenging assays
Source abstract
Abstract
Abstract In this research, the Co precipitation method was utilized to synthesize a nanocomposite of vanadium oxide (V2O5) and graphene oxide (GO). Pure GO was synthesized by the modified hammers method. Using a 1:2 ratio of GO and V2O5 and heating in the oven at 70 °C leading to the formation of V2O5/GO nanocomposite. By grafting of GO on V2O5 surface, a high range of graphene oxidation in V2O5/GO allowed for better reduction with V2O5 metal-oxide. The scanning electron microscope (SEM) images and X-ray diffraction (XRD) spectra provide evidence of the distinct phase of graphene oxide formation. The antioxidant activity of V2O5/GO nanocomposite was conducted in two vitro assays, focusing on neutralization of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and H2O2 radicals. The uniqueness of the nanocomposite was evident from its concentration-dependent antioxidant activities. Interestingly, the V2O5/GO samples has showed 60% antioxidant performance in the H2O2 assays compared to its constituent.
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Research context
OpenAlex topics: Vanadium and Halogenation Chemistry · Nanoparticles: synthesis and applications · Electrochemical Analysis and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (69)
Show source-provided references
1. Novoselov KS et al. (2004) Electric field effect in atomically thin carbon films. Science 306(5696):666–66910.1126/science.1102896 ↗
2. Khan ME, Khan MM, Cho MH (2015) Green synthesis, photocatalytic and photoelectrochemical performance of an Au–graphene nanocomposite. RSC Adv 5(34):26897–2690410.1039/C5RA01864A ↗
3. Ali I et al. (2019) Graphene based adsorbents for remediation of noxious pollutants from wastewater. Environ Int 127:160–18010.1016/j.envint.2019.03.029 ↗
4. Khan ME, Khan MM, Cho MH (2018) Recent progress of metal–graphene nanostructures in photocatalysis. Nanoscale 10(20):9427–944010.1039/C8NR03500H ↗
5. Tadyszak K, Wychowaniec JK, Litowczenko J (2018) Biomedical applications of graphene-based structures. Nanomaterials 8(11):94410.3390/nano8110944 ↗
6. Alam SN, Sharma N, Kumar L (2017) Synthesis of graphene oxide (GO) by modified hummers method and its thermal reduction to obtain reduced graphene oxide (rGO). Graphene 6(1):1–1810.4236/graphene.2017.61001 ↗
7. Marcano DC et al. (2010) Improved synthesis of graphene oxide. ACS Nano 4(8):4806–481410.1021/nn1006368 ↗
8. Omar FS et al. (2014) Microwave synthesis of zinc oxide/reduced graphene oxide hybrid for adsorption-photocatalysis application. Int J Photoenerg 201410.1155/2014/176835 ↗
9. Parnianchi F et al. (2018) Combination of graphene and graphene oxide with metal and metal oxide nanoparticles in fabrication of electrochemical enzymatic biosensors. Int Nano Lett 8(4):229–23910.1007/s40089-018-0253-3 ↗
10. Papageorgiou DG, Kinloch IA, Young RJ (2017) Mechanical properties of graphene and graphene-based nanocomposites. Prog Mater Sci 90:75–12710.1016/j.pmatsci.2017.07.004 ↗
11. Khan ME, Khan MM, Cho MH (2015) Biogenic synthesis of a Ag–graphene nanocomposite with efficient photocatalytic degradation, electrical conductivity and photoelectrochemical performance. N J Chem 39(10):8121–812910.1039/C5NJ01320H ↗
12. Siddeswara D et al. (2016) ZnO decorated graphene nanosheets: an advanced material for the electrochemical performance and photocatalytic degradation of organic dyes. Nanosyst Phys Chem Math 7(4):678–68210.17586/2220-8054-2016-7-4-678-682 ↗
13. Durmus Z, Kurt BZ, Durmus A (2019) Synthesis and characterization of graphene oxide/zinc oxide (GO/ZnO) nanocomposite and its utilization for photocatalytic degradation of basic fuchsin dye. ChemistrySelect 4(1):271–27810.1002/slct.201803635 ↗
14. Baali N et al. (2019) Assessment of antioxidant activity of pure graphene oxide (GO) and ZnO-decorated reduced graphene oxide (rGO) using DPPH radical and H2O2 scavenging assays. C 5(4):75
15. Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181(4617):1199–120010.1038/1811199a0 ↗
16. Ruch RJ, Cheng S-j, Klaunig JE (1989) Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 10(6):1003–100810.1093/carcin/10.6.1003 ↗
17. Crans DC et al. (2011) How environment affects drug activity: localization, compartmentalization and reactions of a vanadium insulin-enhancing compound, dipicolinatooxovanadium (V). Coord Chem Rev 255(19-20):2178–219210.1016/j.ccr.2011.01.032 ↗
18. Goldfine AB et al. (2000) Metabolic effects of vanadyl sulfate in humans with non—insulin-dependent diabetes mellitus: in vivo and in vitro studies. Metabolism 49(3):400–41010.1016/S0026-0495(00)90418-9 ↗
19. Thompson KH, Orvig C (2006) Vanadium in diabetes: 100 years from Phase 0 to Phase I. J Inorg Biochem 100(12):1925–193510.1016/j.jinorgbio.2006.08.016 ↗
20. Zhang Y et al. (2021) Green formulation, chemical characterization, and antioxidant, cytotoxicity, and anti-human cervical cancer effects of vanadium nanoparticles: a pre-clinical study. Arab J Chem 14(6):10314710.1016/j.arabjc.2021.103147 ↗
21. Das S et al. (2020) Anti-angiogenic vanadium pentoxide nanoparticles for the treatment of melanoma and their in vivo toxicity study. Nanoscale 12(14):7604–762110.1039/D0NR00631A ↗
22. Karthik K et al. (2020) Ultrasound-assisted synthesis of V2O5 nanoparticles for photocatalytic and antibacterial studies. Mater Res Innov 24(4):229–23410.1080/14328917.2019.1634404 ↗
23. Chain EE (1991) Optical properties of vanadium dioxide and vanadium pentoxide thin films. Appl Opt 30(19):2782–278710.1364/AO.30.002782 ↗
24. Haber J, Witko M, Tokarz R (1997) Vanadium pentoxide I. Structures and properties. Appl Catal A: Gen 157(1-2):3–2210.1016/S0926-860X(97)00017-3 ↗
25. Roy A, Patra CR (2020) Vanadium pentoxide nanomaterials and their role in anti-angiogenesis for cancer treatment. Future Med 15(27):2643–2646
26. Mailhes JB et al. (2003) Vanadate, an inhibitor of tyrosine phosphatases, induced premature anaphase in oocytes and aneuploidy and polyploidy in mouse bone marrow cells. Mutat Res/Genet Toxicol Environ Mutagen 538(1–2):101–10710.1016/S1383-5718(03)00108-6 ↗
27. Soriano-Agueda LA et al. (2016) Formation of reactive oxygen species by vanadium complexes. Comput Theor Chem 1077:99–10510.1016/j.comptc.2015.11.002 ↗
28. Valko M, Morris H, Cronin M (2005) Metals, toxicity and oxidative stress. Curr Med Chem 12(10):1161–120810.2174/0929867053764635 ↗
29. Shi X, Dalal N (1992) Hydroxyl radical generation in the NADH/microsomal reduction of vanadate. Free Radic Res Commun 17(6):369–37610.3109/10715769209083141 ↗
30. Costa WF, Nepomuceno JC (2006) Protective effects of a mixture of antioxidant vitamins and minerals on the genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster. Environ Mol Mutagen 47(1):18–2410.1002/em.20160 ↗
31. García-Rodríguez MDC, Carvente-Juárez MM, Altamirano-Lozano MA (2013) Antigenotoxic and apoptotic activity of green tea polyphenol extracts on hexavalent chromium-induced DNA damage in peripheral blood of CD-1 mice: analysis with differential acridine orange/ethidium bromide staining. Oxidative Med Cell Longev 2013:48641910.1155/2013/486419 ↗
32. Antunes LMG, Takahashi CS (1998) Effects of high doses of vitamins C and E against doxorubicin-induced chromosomal damage in Wistar rat bone marrow cells. Mutat Res/Genet Toxicol Environ Mutagen 419(1-3):137–14310.1016/S1383-5718(98)00134-X ↗
33. Siddique YH, Beg T, Afzal M (2005) Antigenotoxic effects of ascorbic acid against megestrol acetate-induced genotoxicity in mice. Hum Exp Toxicol 24(3):121–12710.1191/0960327104ht508oa ↗
34. Singh M et al. (2008) Protective effects of vitamin E against atrazine-induced genotoxicity in rats. Mutat Res/Genet Toxicol Environ Mutagen 654(2):145–14910.1016/j.mrgentox.2008.05.010 ↗
35. Head KA (1998) Ascorbic acid in the prevention and treatment of cancer. Alter Med Rev 3(3):174–186
36. Block G (1991) Vitamin C and cancer prevention: the epidemiologic evidence. Am J Clin Nutr 53(1):270S–282S10.1093/ajcn/53.1.270S ↗
37. Frei B (1994) Reactive oxygen species and antioxidant vitamins: mechanisms of action. Am J Med 97(3):S5–S1310.1016/0002-9343(94)90292-5 ↗
38. Naidu KA (2003) Vitamin C in human health and disease is still a mystery? An overview. Nutr J 2(1):1–1010.1186/1475-2891-2-7 ↗
39. Lunec J et al. (2004) Effect of vitamin E on gene expression changes in diet‐related carcinogenesis. Ann NY Acad Sci 1031(1):169–18310.1196/annals.1331.016 ↗
40. Sugiyama M et al. (1992) Effect of vitamin E on cytotoxicity, DNA single strand breaks, chromosomal aberrations, and mutation in chinese hamster V‐79 cells exposed to ultraviolet‐B light. Photochem Photobiol 56(1):31–3410.1111/j.1751-1097.1992.tb09598.x ↗
41. Kayden HJ, Traber MG (1993) Absorption, lipoprotein transport, and regulation of plasma concentrations of vitamin E in humans. J lipid Res 34(3):343–35810.1016/S0022-2275(20)40727-8 ↗
42. Packer L (1991) Protective role of vitamin E in biological systems. Am J Clin Nutr 53(4):1050S–1055S10.1093/ajcn/53.4.1050S ↗
43. Haber SL, Wissler RW (1962) Effect of vitamin E on carcinogenicity of methylcholanthrene. Proc Soc Exp Biol Med 111(3):774–77510.3181/00379727-111-27918 ↗
44. Ogutcu A et al. (2006) The effects of organophosphate insecticide diazinon on malondialdehyde levels and myocardial cells in rat heart tissue and protective role of vitamin E. Pestic Biochem Physiol 86(2):93–9810.1016/j.pestbp.2006.01.010 ↗
45. García-Rodríguez M, Serrano-Reyes G, Altamirano-Lozano M (2012) Comparative study in vivo of the genotoxic damage induced by CrO3 and the effects of the antioxidants: Ascorbic acid, alfa-tocopherol and beta-carotene. Free Radic Biol Med 53:S21610.1016/j.freeradbiomed.2012.08.453 ↗
46. Yusoff F et al. (2018) The electrochemical behavior of zinc oxide/reduced graphene oxide composite electrode in dopamine. Malays J Anal Sci 22(2):227–237
47. Zaaba N et al. (2017) Synthesis of graphene oxide using modified hummers method: solvent influence. Procedia Eng 184:469–47710.1016/j.proeng.2017.04.118 ↗
48. Akhtar N et al. (2021) Synthesis and characterization of MXene/BiCr2O4 nanocomposite with excellent electrochemical properties. J Mater Res Technol 15:2007–201510.1016/j.jmrt.2021.08.101 ↗
49. Gupta J et al. (2018) Superparamagnetic iron oxide-reduced graphene oxide nanohybrid-a vehicle for targeted drug delivery and hyperthermia treatment of cancer. J Magn Magn Mater 448:332–33810.1016/j.jmmm.2017.05.084 ↗
50. Li S et al. (2019) One-step co-precipitation method to construct black phosphorus nanosheets/ZnO nanohybrid for enhanced visible light photocatalytic activity. Appl Surf Sci 497:14368210.1016/j.apsusc.2019.143682 ↗
51. Kim K-S, Park S-J (2011) Influence of multi-walled carbon nanotubes on the electrochemical performance of graphene nanocomposites for supercapacitor electrodes. Electrochim Acta 56(3):1629–163510.1016/j.electacta.2010.10.043 ↗
52. Zhong X et al. (2010) Aryne cycloaddition: highly efficient chemical modification of graphene. Chem Commun 46(39):7340–734210.1039/c0cc02389b ↗
53. Munir N et al (2020) Experimental investigation of low dimensional spin system in metal oxides. J Nanoscope 1(1):45–64
54. Shafeeq K et al. (2020) Structural and optical properties of V2O5 nanostructures grown by thermal decomposition technique. Appl Phys A 126:1–610.1007/s00339-020-03770-5 ↗
55. Tubthong T et al. (2018) Development of ammonia gas sensor based on Ni-doped reduce graphene oxide. in MATEC Web of Conferences. EDP Sciences.10.1051/matecconf/201819202048 ↗
56. Vinodhkumar G et al. (2021) One step solvothermal synthesis and characterization of rGO/NiO nanocomposites. Mater Today Proc 35:17–2210.1016/j.matpr.2019.06.004 ↗
57. Batool K et al. (2021) Sintering and property characterization of copper doped strontium chromite by sol gel method. J Nanoscope 2(2):215–23010.52700/jn.v2i2.35 ↗
58. Zhao H et al. (2013) Vanadium oxides–reduced graphene oxide composite for lithium-ion batteries and supercapacitors with improved electrochemical performance. J Power Sources 222:21–3110.1016/j.jpowsour.2012.08.036 ↗
59. Xing X et al. (2018) Manganese vanadium oxide–N-doped reduced graphene oxide composites as oxygen reduction and oxygen evolution electrocatalysts. ACS Appl Mater Interfaces 10(51):44511–4451710.1021/acsami.8b16578 ↗
60. Rania M et al. (2021) Sol-gel synthesis of nanoscaled spinels using 1, 2-ethanediol as a gelation agent. J Optoelectron Biomed Mater 13(3):89–9410.15251/JOBM.2021.133.89 ↗
61. Qiu Y et al. (2014) Antioxidant chemistry of graphene-based materials and its role in oxidation protection technology. Nanoscale 6(20):11744–1175510.1039/C4NR03275F ↗
62. Liu X et al. (2011) Antioxidant deactivation on graphenic nanocarbon surfaces. Small 7(19):2775–278510.1002/smll.201100651 ↗
63. Radich EJ, Kamat PV (2013) Making graphene holey. Gold-nanoparticle-mediated hydroxyl radical attack on reduced graphene oxide. ACS Nano 7(6):5546–555710.1021/nn401794k ↗
64. Suresh D et al. (2015) Cinnamon supported facile green reduction of graphene oxide, its dye elimination and antioxidant activities. Mater Lett 151:93–9510.1016/j.matlet.2015.03.035 ↗
65. Fatima F, Singh HR, Jha SK (2021) Assessment of antioxidant and cytotoxicity activities against A-549 lung cancer cell line by synthesized reduced graphene oxide nanoparticles mediated by Camellia sinensis. 3 Biotech 11(12):1–1410.1007/s13205-021-03015-z ↗
66. Alves Z, Nunes C, Ferreira (2021) Unravelling the role of synthesis conditions on the structure of zinc oxide-reduced graphene oxide nanofillers. Nanomaterials 11(8):214910.3390/nano11082149 ↗
67. Suresh D, Nagabhushana H, Sharma S (2015) Clove extract mediated facile green reduction of graphene oxide, its dye elimination and antioxidant properties. Mater Lett 142:4–610.1016/j.matlet.2014.11.073 ↗
68. Suresh D et al. (2015) Spinach assisted green reduction of graphene oxide and its antioxidant and dye absorption properties. Ceram Int 41(3):4810–481310.1016/j.ceramint.2014.12.036 ↗
69. Jin X et al. (2018) Green reduction of graphene oxide using eucalyptus leaf extract and its application to remove dye. Chemosphere 208:417–42410.1016/j.chemosphere.2018.05.199 ↗