Spheroidal and nanocrystal structures created from carbodiimide crosslinking reaction with RADA16
Source abstract
Abstract
RADA16 is a widely studied polypeptide known for its ability to self-assemble into β -sheets that form nanofibers. Here we show that it is possible to self-crosslink the molecule via 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) as aqueous solutions. The product results in a mix of nanocrystals and near micron-size spherules. SEM and TEM pictures provide a view of the structures and nano tracking analysis gives their size distributions. FTIR analysis provides evidence for the existence of a crosslinking reaction.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Supramolecular Self-Assembly in Materials · Silk-based biomaterials and applications · Polymer Surface Interaction Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (13)
Show source-provided references
1. Spatial distribution of mineralized bone matrix produced by marrow mesenchymal stem cells in self-assembling peptide hydrogel scaffold10.1002/jbm.a.31439 ↗
2. Primary sequence of ionic self-assembling peptide gels affects endothelial cell adhesion and capillary morphogenesis10.1002/jbm.a.31785 ↗
3. 3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds10.2147/IJN.S17292 ↗
4. Solid state self-assembly mechanism of RADA 16-I designer peptide10.1021/bm300313h ↗
5. Designer self-assembling peptide nanofibers10.1021/nn401562f ↗
6. End-to-end self-assembly of RADA 16-I nanofibrils in aqueous solutions10.1016/j.bpj.2012.03.012 ↗
7. Bone induction by biomimetic PLGA copolymer loaded with a novel synthetic RADA16-P24 peptide in vivo10.1016/j.msec.2013.04.019 ↗
8. Nanocrystal technology, drug delivery and clinical applications
9. ThermoFisherScientific, Edc (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride), User Guide: EDC (Accessed 8 December 2015) (c2011) https://www.thermofisher.com.
10. RADA-16: a tough peptide strategies for synthesis and purification10.1002/ejoc.201300612 ↗
11. Infrared spectroscopy of proteins10.1016/j.bbabio.2007.06.004 ↗
12. A convenient synthesis of water-soluble carbodiimides10.1021/jo01351a600 ↗
13. Cascade of transitions of polyelectrolytes in poor solvents10.1021/ma9507958 ↗