journal article·2026-06-24

3D printed silk fibroin-bentonite-hydroxyapatite hybrid scaffold with antioxidant property for bone regeneration

Logeshwaran A, Renold Elsen, Ashutosh D. Bagde, Sunita Nayak

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OpenAlex topics: Silk-based biomaterials and applications · Bone Tissue Engineering Materials · 3D Printing in Biomedical Research

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References (66)

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1. Y. Seol, H. Kang, S.J. Lee, A. Atala, J.J. Yoo, Bioprinting technology and its applications, pp. 1–7, 2014, https://doi.org/10.1093/ejcts/ezu14810.1093/ejcts/ezu148 ↗
2. ASzwed-Georgiou et al., Bioactive Materials for Bone Regeneration: Biomolecules and Delivery Systems. ACS Biomater. Sci. Eng. 9(9), 5222–5254 (2023). https://doi.org/10.1021/ACSBIOMATERIALS.3C00609/ASSET/IMAGES/LARGE/AB3C00609_0003.JPEG10.1021/ACSBIOMATERIALS.3C00609 ↗
3. M.M. Germaini, S. Belhabib, S. Guessasma, R. Deterre, P. Corre, P. Weiss, Additive manufacturing of biomaterials for bone tissue engineering – A critical review of the state of the art and new concepts. Prog Mater. Sci. 130, 100963 (2022). https://doi.org/10.1016/J.PMATSCI.2022.10096310.1016/J.PMATSCI.2022.100963 ↗
4. G. Lee, M. Carrillo, J. McKittrick, D.G. Martin, E.A. Olevsky, Fabrication of ceramic bone scaffolds by solvent jetting 3D printing and sintering: Towards load-bearing applications. Addit. Manuf. 33, 101107 (2020). https://doi.org/10.1016/J.ADDMA.2020.10110710.1016/J.ADDMA.2020.101107 ↗
5. H. Budharaju et al., Ceramic materials for 3D printing of biomimetic bone scaffolds – Current state-of-the-art & future perspectives. Mater. Des. 231, 112064 (2023). https://doi.org/10.1016/J.MATDES.2023.11206410.1016/J.MATDES.2023.112064 ↗
6. H.L. Jaber, T.A. Kovács, Preparation and synthesis of hydroxyapatite bio-ceramic from bovine bone by thermal heat treatment. Epitoanyag - J. Silicate Based Compos. Mater. 71(3), 98–101 (2019). https://doi.org/10.14382/epitoanyag-jsbcm.2019.1810.14382/epitoanyag-jsbcm.2019.18 ↗
7. K. Dave, V.G. Gomes, Interactions at scaffold interfaces: Effect of surface chemistry, structural attributes and bioaffinity. Mater. Sci. Engineering: C 105, 110078 (2019). https://doi.org/10.1016/J.MSEC.2019.11007810.1016/J.MSEC.2019.110078 ↗
8. M. Tallawi et al., Strategies for the chemical and biological functionalization of scaffolds for cardiac tissue engineering: a review. J. R Soc. Interface. 12, 20150254 (2015). https://doi.org/10.1098/RSIF.2015.025410.1098/RSIF.2015.0254 ↗
9. S. Du et al., Bioactive polymer composite scaffolds fabricated from 3D printed negative molds enable bone formation and vascularization. Acta Biomater. 186, 260–274 (2024). https://doi.org/10.1016/J.ACTBIO.2024.07.03810.1016/J.ACTBIO.2024.07.038 ↗
10. L. Hodásová et al., Polymer infiltrated ceramic networks with biocompatible adhesive and 3D-printed highly porous scaffolds. Addit. Manuf. 39, 101850 (2021). https://doi.org/10.1016/J.ADDMA.2021.10185010.1016/J.ADDMA.2021.101850 ↗
11. F.J. Martínez-Vázquez, F.H. Perera, P. Miranda, A. Pajares, F. Guiberteau, Improving the compressive strength of bioceramic robocast scaffolds by polymer infiltration. Acta Biomater. 6(11), 4361–4368 (2010). https://doi.org/10.1016/J.ACTBIO.2010.05.02410.1016/J.ACTBIO.2010.05.024 ↗
12. X. Shi et al., 3D printed intelligent scaffold prevents recurrence and distal metastasis of breast cancer. Theranostics. 10, 10652 (2020). https://doi.org/10.7150/THNO.4793310.7150/THNO.47933 ↗
13. JE.Song, N. Tripathy, D.H. Lee, J.H. Park, G. Khang, Quercetin Inlaid Silk Fibroin/Hydroxyapatite Scaffold Promotes Enhanced Osteogenesis. ACS Appl. Mater. Interfaces. 10(39), 32955–32964 (2018). https://doi.org/10.1021/ACSAMI.8B08119/ASSET/IMAGES/MEDIUM/AM-2018-081198_0006.GIF10.1021/ACSAMI.8B08119/ASSET ↗
14. E. Wenk, H.P. Merkle, L. Meinel, Silk fibroin as a vehicle for drug delivery applications. J. Controlled Release. 150(2), 128–141 (2011). https://doi.org/10.1016/J.JCONREL.2010.11.00710.1016/J.JCONREL.2010.11.007 ↗
15. SA.L. Matthew, F.P. Seib, Silk Bioconjugates: From Chemistry and Concept to Application. ACS Biomater. Sci. Eng. 10(1), 12–28 (2024). https://doi.org/10.1021/ACSBIOMATERIALS.2C01116/ASSET/IMAGES/LARGE/AB2C01116_0006.JPEG10.1021/ACSBIOMATERIALS.2C01116/ASSET ↗
16. M.A. Sakr et al., Development of bentonite-gelatin nanocomposite hybrid hydrogels for tissue engineering. Appl. Clay Sci. 199, 105860 (2020). https://doi.org/10.1016/J.CLAY.2020.10586010.1016/J.CLAY.2020.105860 ↗
17. C. Shuai, W. Yang, P. Feng, S. Peng, H. Pan, Accelerated degradation of HAP/PLLA bone scaffold by PGA blending facilitates bioactivity and osteoconductivity. Bioact Mater. 6(2), 490–502 (2021). https://doi.org/10.1016/j.bioactmat.2020.09.00110.1016/j.bioactmat.2020.09.001 ↗
18. B.Banimohamad-Shotorbani, A. Rahmani, D.A. Bakhshayesh, S. Mehdipour, H. Jarolmasjed, Shafaei, The efficiency of PCL/HAp electrospun nanofibers in bone regeneration: a review. 45(7), 511–531 (2021). https://doi.org/10.1080/03091902.2021.189339610.1080/03091902.2021.1893396 ↗
19. A. Logeshwaran, R. Elsen, S. Nayak, Mechanical and biological characteristics of 3D fabricated clay mineral and bioceramic composite scaffold for bone tissue applications. J. Mech. Behav. Biomed. Mater. 138, 105633 (2023). https://doi.org/10.1016/j.jmbbm.2022.10563310.1016/j.jmbbm.2022.105633 ↗
20. T. Zhou, Y. Yan, C. Zhao, Y. Xu, Q. Wang, N. Xu, Resveratrol improves osteogenic differentiation of senescent bone mesenchymal stem cells through inhibiting endogenous reactive oxygen species production via AMPK activation. Redox Rep. 24(1), 62–69 (2019). https://doi.org/10.1080/13510002.2019.165837610.1080/13510002.2019.1658376 ↗
21. S. Abdulrahman Hussain et al., Efficacy and safety of co-administration of resveratrol with meloxicam in patients with knee osteoarthritis: A pilot interventional study. Clin. Interv Aging. 13, 1621–1630 (2018). https://doi.org/10.2147/CIA.S17275810.2147/CIA.S172758 ↗
22. R. Hameister, C. Kaur, S.T. Dheen, C.H. Lohmann, G. Singh, Reactive oxygen/nitrogen species (ROS/RNS) and oxidative stress in arthroplasty. J. Biomed. Mater. Res. B Appl. Biomater. 108(5), 2073–2087 (2020). https://doi.org/10.1002/JBM.B.3454610.1002/JBM.B.34546 ↗
23. A.E. Bădilă, D.M. Rădulescu, A. Ilie, A.G. Niculescu, A.M. Grumezescu, A.R. Rădulescu, Bone Regeneration and Oxidative Stress: An Updated Overview. Antioxid. Basel. 11(2) (2022). https://doi.org/10.3390/ANTIOX1102031810.3390/ANTIOX11020318 ↗
24. H. Hu et al., Regulation of hypoxic stress and oxidative stress in bone grafting: Current trends and future perspectives. J. Mater. Sci. Technol. 157, 144–153 (2023). https://doi.org/10.1016/j.jmst.2023.01.05510.1016/j.jmst.2023.01.055 ↗
25. M.F. Manchope et al., Naringenin mitigates titanium dioxide (TiO 2)-induced chronic arthritis in mice: role of oxidative stress, cytokines, and NFκB. Inflamm. Res. 67, 11–12 (2018). https://doi.org/10.1007/S00011-018-1195-Y/METRICS10.1007/S00011-018-1195-Y/METRICS ↗
26. T. yang Wang, Q. Li, K. shun, Bi, Bioactive flavonoids in medicinal plants: Structure, activity and biological fate. Asian J. Pharm. Sci. 13(1), 12–23 (2018). https://doi.org/10.1016/J.AJPS.2017.08.00410.1016/J.AJPS.2017.08.004 ↗
27. S. Chakravarty, N. Bhardwaj, B.B. Mandal, N. Sen Sarma, Silk fibroin-carbon nanoparticle composite scaffolds: A cost effective supramolecular ‘turn off’ chemiresistor for nitroaromatic explosive vapours. J. Mater. Chem. C Mater. 4(38), 8920–8929 (2016). https://doi.org/10.1039/C6TC03337G10.1039/C6TC03337G ↗
28. I. D’Onofrio et al., Inhalable drug-loaded silk fibroin carriers for pulmonary drug delivery. RSC Adv. 14(37), 27288–27297 (2024). https://doi.org/10.1039/D4RA03324H10.1039/D4RA03324H ↗
29. D. Xue et al., The role of hesperetin on osteogenesis of human mesenchymal stem cells and its function in bone regeneration. Oncotarget. 8, 21031 (2017). https://doi.org/10.18632/ONCOTARGET.1547310.18632/ONCOTARGET.15473 ↗
30. P.A. Miguez et al., Hesperidin Promotes Osteogenesis and Modulates Collagen Matrix Organization and Mineralization In Vitro and In Vivo. Int. J. Mol. Sci. 22(6), 1–13 (2021). https://doi.org/10.3390/IJMS2206322310.3390/IJMS22063223 ↗
31. K.H. Huang, C.Y. Chen, C.Y. Chang, Y.W. Chen, C.P. Lin, The synergistic effects of quercetin-containing 3D-printed mesoporous calcium silicate/calcium sulfate/poly-ε-caprolactone scaffolds for the promotion of osteogenesis in mesenchymal stem cells. J. Formos. Med. Assoc. (2021). https://doi.org/10.1016/j.jfma.2021.01.02410.1016/j.jfma.2021.01.024 ↗
32. (ASTM), ASTM D7334-08 Standard Practice for Surface Wettability of Coatings, Substrates and Pigments by Advancing Contact Angle Measurement. ASTM Int. 08, 1–3 (2008). https://doi.org/10.1520/D7334-08R22.Copyright10.1520/D7334-08R22.Copyright ↗
33. M.N. Gómez-Cerezo, D. Lozano, D. Arcos, M. Vallet-Regí, C. Vaquette, The effect of biomimetic mineralization of 3D-printed mesoporous bioglass scaffolds on physical properties and in vitro osteogenicity. Mater. Sci. Eng. C 109, 110572 (2020). https://doi.org/10.1016/J.MSEC.2019.11057210.1016/J.MSEC.2019.110572 ↗
34. X. Zhang et al., Three-Dimensional Printed Cell Culture Model Based on Spherical Colloidal Lignin Particles and Cellulose Nanofibril-Alginate Hydrogel. Biomacromolecules. 21(5), 1875–1885 (2020). https://doi.org/10.1021/acs.biomac.9b0174510.1021/acs.biomac.9b01745 ↗
35. G.E. Fawal, H. Hong, X. Mo, H. Wang, Fabrication of scaffold based on gelatin and polycaprolactone (PCL) for wound dressing application. J. Drug Deliv Sci. Technol. 63, 102501 (2021). https://doi.org/10.1016/j.jddst.2021.10250110.1016/j.jddst.2021.102501 ↗
36. K.M. Sadek, W. Mamdouh, S.I. Habib, M.E. Deftar, A.N.A. Habib, In Vitro Biological Evaluation of a Fabricated Polycaprolactone/Pomegranate Electrospun Scaffold for Bone Regeneration, 2021. https://doi.org/10.1021/acsomega.1c0460810.1021/acsomega.1c04608 ↗
37. J.Y. Lee et al., Design of a 3D BMP-2-delivering tannylated PCL scaffold and its anti-oxidant, anti-inflammatory, and osteogenic effects in vitro, 2018. https://doi.org/10.3390/ijms1911360210.3390/ijms19113602 ↗
38. Astm, "F 813-01: Standard Practice for Direct Contact Cell Culture Evaluation of Materials for," 24, 1652–1653 (2001). https://doi.org/10.1520/F0813-20.110.1520/F0813-20.1 ↗
39. Z. Xu, Y. Sun, H. Dai, Y. Ma, H. Bing, Engineered 3D-Printed Polyvinyl Alcohol Scaffolds Incorporating β-Tricalcium Phosphate and Icariin Induce Bone Regeneration in Rat Skull Defect Model. Molecules. 27(14) (2022). https://doi.org/10.3390/MOLECULES2714453510.3390/MOLECULES27144535 ↗
40. W. Liu et al., Electrospun porous poly (3-hydroxybutyrate-co-4-hydroxybutyrate)/lecithin scaffold for bone tissue engineering. RSC Adv. 12(19), 11913–11922 (2022)10.1039/D2RA01398C ↗
41. K. Pal et al., Folic acid conjugated curcumin loaded biopolymeric gum acacia microsphere for triple negative breast cancer therapy in invitro and invivo model. Mater. Sci. Eng. C 95, 204–216 (2019). https://doi.org/10.1016/j.msec.2018.10.07110.1016/j.msec.2018.10.071 ↗
42. P. Kumar, I. Rupmanjari, K. Ajay, R.P. Mariadhas, V. Arasu, Insulin signaling pathway assessment by enhancing antioxidant activity due to morin using in vitro rat skeletal muscle L6 myotubes cells. Mol. Biol. Rep. 48(8), 5857–5872 (2021). https://doi.org/10.1007/s11033-021-06580-x10.1007/s11033-021-06580-x ↗
43. H. Hong et al., Cytocompatibility of modified silk fibroin with glycidyl methacrylate for tissue engineering and biomedical applications. Biomolecules. 11(1), 1–18 (2021). https://doi.org/10.3390/BIOM1101003510.3390/BIOM11010035 ↗
44. S. Das, D. Pati, N. Tiwari, A. Nisal, S. Sen Gupta, Synthesis of silk fibroin-glycopolypeptide conjugates and their recognition with lectin. Biomacromolecules. 13(11), 3695–3702 (2012). https://doi.org/10.1021/BM301170U/SUPPL_FILE/BM301170U_SI_001.PDF10.1021/BM301170U/SUPPL_FILE/BM301170U_SI_001.PDF ↗
45. E.T. Baran, K. Tuzlakoǧlu, J.F. Mano, R.L. Reis, Enzymatic degradation behavior and cytocompatibility of silk fibroin–starch–chitosan conjugate membranes. Mater. Sci. Engineering: C 32(6), 1314–1322 (2012). https://doi.org/10.1016/J.MSEC.2012.02.01510.1016/J.MSEC.2012.02.015 ↗
46. R. Ferracini, I. Martínez Herreros, A. Russo, T. Casalini, F. Rossi, G. Perale, Scaffolds as Structural Tools for Bone-Targeted Drug Delivery. Pharm. 10(3), (2018). https://doi.org/10.3390/PHARMACEUTICS1003012210.3390/PHARMACEUTICS10030122 ↗
47. R.Deshpande, S. Shukla, A. Kale, N. Deshmukh, A. Nisal, P. Venugopalan, Silk Fibroin Microparticle Scaffold for Use in Bone Void Filling: Safety and Efficacy Studies. ACS Biomater. Sci. Eng. 8(3), 1226–1238 (2022). https://doi.org/10.1021/ACSBIOMATERIALS.1C01103/SUPPL_FILE/AB1C01103_SI_001.PDF10.1021/ACSBIOMATERIALS.1C01103 ↗
48. A. Logeshwaran, R. Elsen, S. Nayak, Mechanical and biological characteristics of 3D fabricated clay mineral and bioceramic composite scaffold for bone tissue applications. J. Mech. Behav. Biomed. Mater. 138, 105633 (2023). https://doi.org/10.1016/J.JMBBM.2022.10563310.1016/J.JMBBM.2022.105633 ↗
49. K.A. Burke, D.C. Roberts, D.L. Kaplan, Silk Fibroin Aqueous-Based Adhesives Inspired by Mussel Adhesive Proteins. Biomacromolecules. 17(1), 237 (2016). https://doi.org/10.1021/ACS.BIOMAC.5B0133010.1021/acs.biomac.5b01330 ↗
50. S. Kaushik, P.D. Thungon, P. Goswami, Silk Fibroin: an emerging biocompatible material for application of enzymes and whole cells in bioelectronics and bioanalytical sciences. ACS Biomater. Sci. Eng. 6(8), 4337–4355 (2020). https://doi.org/10.1021/ACSBIOMATERIALS.9B01971/ASSET/IMAGES/MEDIUM/AB9B01971_0009.GIF10.1021/ACSBIOMATERIALS.9B01971/ASSET ↗
51. X. Wu, K. Walsh, B.L. Hoff, G. Camci-Unal, Mineralization Biomaterials Bone Tissue Eng. Bioeng. 7(4), 132 (2020). https://doi.org/10.3390/BIOENGINEERING704013210.3390/BIOENGINEERING7040132 ↗
52. K. Rodríguez, J. Sundberg, P. Gatenholm, S. Renneckar, Electrospun nanofibrous cellulose scaffolds with controlled microarchitecture. Carbohydr. Polym. 100, 143–149 (2014). https://doi.org/10.1016/J.CARBPOL.2012.12.03710.1016/J.CARBPOL.2012.12.037 ↗
53. Y. Jin, B. Kundu, Y. Cai, S.C. Kundu, J. Yao, Bio-inspired mineralization of hydroxyapatite in 3D silk fibroin hydrogel for bone tissue engineering. Colloids Surf. B Biointerfaces. 134, 339–345 (2015). https://doi.org/10.1016/J.COLSURFB.2015.07.01510.1016/J.COLSURFB.2015.07.015 ↗
54. Z.J. Chen et al., Mineralized self-assembled silk fibroin/cellulose interpenetrating network aerogel for bone tissue engineering. Biomaterials Adv. 134, 112549 (2022). https://doi.org/10.1016/J.MSEC.2021.11254910.1016/J.MSEC.2021.112549 ↗
55. P. Xiong Sep., B. Ca et al., Sr/P-doped silk fibroin films on Mg-1Ca alloy with dramatic corrosion resistance and osteogenic activities. ACS Biomater. Sci. Eng. 4(9), 3163–3176 (2018). https://doi.org/10.1021/ACSBIOMATERIALS.8B0078710.1021/ACSBIOMATERIALS.8B00787 ↗
56. I. Nugrahani, M.A. Jessica, Amino acids as the potential co-former for co-crystal development: a review. Molecules. 26(11), 3279 (2021). https://doi.org/10.3390/MOLECULES2611327910.3390/MOLECULES26113279 ↗
57. M. Olszowy, A.L. Dawidowicz, Is it possible to use the DPPH and ABTS methods for reliable estimation of antioxidant power of colored compounds? Chem. Pap. 72(2), 393–400 (2018). https://doi.org/10.1007/S11696-017-0288-3/TABLES/310.1007/S11696-017-0288-3/TABLES/3 ↗
58. K.J. Lee, Y.C. Oh, W.K. Cho, J.Y. Ma, Antioxidant and anti-inflammatory activity determination of one hundred kinds of pure chemical compounds using offline and online screening HPLC assay. Evid. Based Complement. Alternat Med. 2015, 165457 (2015). https://doi.org/10.1155/2015/16545710.1155/2015/165457 ↗
59. J. Rumpf, R. Burger, M. Schulze, Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins. Int. J. Biol. Macromol. 233, 123470 (2023). https://doi.org/10.1016/J.IJBIOMAC.2023.12347010.1016/J.IJBIOMAC.2023.123470 ↗
60. C.M.C. Andrés, J.M. Pérez de la Lastra, C.A. Juan, F.J. Plou, E. Pérez-Lebeña, Polyphenols as antioxidant/pro-oxidant compounds and donors of reducing species: relationship with human antioxidant metabolism. Processes 2023. 11(9), 2771 (2023). https://doi.org/10.3390/PR1109277110.3390/PR11092771 ↗
61. J. Zhang et al., The Role of Flavonoids in the Osteogenic Differentiation of Mesenchymal Stem Cells. Front. Pharmacol. 13, 849513 (2022). https://doi.org/10.3389/FPHAR.2022.84951310.3389/FPHAR.2022.849513 ↗
62. M.S. Shojaee, M. Moeenfard, R. Farhoosh, Kinetics and stoichiometry of gallic acid and methyl gallate in scavenging DPPH radical as affected by the reaction solvent. Scientific Reports 2022. 12(1), 1–9 (2022). https://doi.org/10.1038/s41598-022-12803-310.1038/s41598-022-12803-3 ↗
63. J. Jodynis-liebert, M. Kujawska, Biphasic dose-response induced by phytochemicals: experimental evidence. J. Clin. Med. 9(3), 718 (2020). https://doi.org/10.3390/JCM903071810.3390/JCM9030718 ↗
64. X. Xi, J. Wang, Y. Qin, Y. You, W. Huang, J. Zhan, The biphasic effect of flavonoids on oxidative stress and cell proliferation in breast cancer cells. Antioxidants. 11(4), 622 (2022). https://doi.org/10.3390/ANTIOX11040622/S110.3390/ANTIOX11040622/S1 ↗
65. A. Deb, How stem cells turn into bone and fat. N Engl. J. Med. 380(23), 2268 (2019). https://doi.org/10.1056/NEJMCIBR190516510.1056/NEJMCIBR1905165 ↗
66. M. Ponzetti, N. Rucci, Osteoblast differentiation and signaling: established concepts and emerging topics. Int. J. Mol. Sci. 22, 6651 (2021). https://doi.org/10.3390/IJMS2213665110.3390/IJMS22136651 ↗