Fluorogenic Thiazole Orange Dimer for Recognition of G-Quadruplex-Duplex Hybrid and G-Quadruplex DNA
Source abstract
Abstract
A collection of oxyethylene-bridged thiazole orange dimers with systematically varied spacer lengths was synthesized for the fluorogenic detection of higher-order DNA structures. Spectroscopic studies reveal that spacer length critically regulates binding and signal amplification. TO4TO exhibits maximum fluorescence turn-on and selectively detects parallel G-quadruplex and quadruplex-duplex hybrid DNA with nanomolar sensitivity. Notably, it can serve as a cellular imaging probe, demonstrating that spacer engineering is an effective strategy for fluorogenic detection and imaging of higher-order DNA structures.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: DNA and Nucleic Acid Chemistry · Advanced biosensing and bioanalysis techniques · RNA Interference and Gene Delivery
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
School of Applied and Interdisciplinary Sciences
Indian Association for the Cultivation of Science
Department of Chemistry
Indian Institute of Science Education and Research Tirupati
Department of Organic Chemistry
Indian Institute of Science
Funding
Indian Institute of Science Education and Research (IISER) Tirupati · Council of Scientific and Industrial Research (CSIR) · J. C. Bose Fellowship, Department of Science and Technology (DST), India · Indian Association for the Cultivation of Science (IACS)
References (72)
Show source-provided references
1. 10.1021/jacs.3c1385110.1021/jacs.3c13851 ↗
2. 10.1021/acssensors.3c0006810.1021/acssensors.3c00068 ↗
3. 10.1021/acs.jpcb.5c0714510.1021/acs.jpcb.5c07145 ↗
4. 10.1021/acsabm.5c0193510.1021/acsabm.5c01935 ↗
5. 10.1021/acs.jpclett.2c0330110.1021/acs.jpclett.2c03301 ↗
6. 10.1016/j.biochi.2025.04.00410.1016/j.biochi.2025.04.004 ↗
7. 10.1021/acs.orglett.5b0231010.1021/acs.orglett.5b02310 ↗
8. 10.1021/acs.jpclett.9b0063710.1021/acs.jpclett.9b00637 ↗
9. 10.1021/acs.bioconjchem.3c0011410.1021/acs.bioconjchem.3c00114 ↗
10. 10.1039/C7OB02326J10.1039/C7OB02326J ↗
11. 10.1093/nar/gkaf89410.1093/nar/gkaf894 ↗
12. 10.1016/j.jinorgbio.2025.11294710.1016/j.jinorgbio.2025.112947 ↗
13. 10.1016/j.jbc.2025.11089710.1016/j.jbc.2025.110897 ↗
14. 10.1126/science.adr175210.1126/science.adr1752 ↗
15. 10.1021/ol302918f10.1021/ol302918f ↗
16. 10.1039/D5CC03633J10.1039/D5CC03633J ↗
17. 10.1021/acsptsci.5c0040810.1021/acsptsci.5c00408 ↗
18. 10.1093/nar/gkaa75210.1093/nar/gkaa752 ↗
19. 10.1021/acs.jcim.1c0150010.1021/acs.jcim.1c01500 ↗
20. 10.1016/j.jinorgbio.2025.11290410.1016/j.jinorgbio.2025.112904 ↗
21. 10.1039/D4AN01454E10.1039/D4AN01454E ↗
22. 10.1093/nar/gky66510.1093/nar/gky665 ↗
23. 10.1016/j.snb.2024.13676810.1016/j.snb.2024.136768 ↗
24. 10.1021/jacs.3c1190810.1021/jacs.3c11908 ↗
25. 10.1002/anie.20242493110.1002/anie.202424931 ↗
26. 10.1021/acs.analchem.1c0455610.1021/acs.analchem.1c04556 ↗
27. 10.1038/s41467-020-20414-710.1038/s41467-020-20414-7 ↗
28. 10.1021/acs.accounts.2c0075710.1021/acs.accounts.2c00757 ↗
29. 10.1016/j.jphotobiol.2018.10.00710.1016/j.jphotobiol.2018.10.007 ↗
30. 10.1016/j.dyepig.2022.11030710.1016/j.dyepig.2022.110307 ↗
31. 10.1080/1525777070154821210.1080/15257770701548212 ↗
32. 10.1016/j.dyepig.2025.11340410.1016/j.dyepig.2025.113404 ↗
33. 10.1016/j.talanta.2025.12875710.1016/j.talanta.2025.128757 ↗
34. 10.1016/j.dyepig.2025.11351310.1016/j.dyepig.2025.113513 ↗
35. 10.1039/D2AN01804G10.1039/D2AN01804G ↗
36. 10.1016/j.ijbiomac.2022.12.23110.1016/j.ijbiomac.2022.12.231 ↗
37. 10.1021/acs.jmedchem.0c0165610.1021/acs.jmedchem.0c01656 ↗
38. 10.1021/acsabm.9b0124310.1021/acsabm.9b01243 ↗
39. 10.1021/acsomega.0c0355610.1021/acsomega.0c03556 ↗
40. 10.1016/j.dyepig.2015.11.01010.1016/j.dyepig.2015.11.010 ↗
41. 10.1016/j.dyepig.2016.10.02210.1016/j.dyepig.2016.10.022 ↗
42. 10.1021/ac300207r10.1021/ac300207r ↗
43. 10.1021/acsomega.8b0343010.1021/acsomega.8b03430 ↗
44. 10.1021/acschembio.5b0098710.1021/acschembio.5b00987 ↗
45. 10.1021/acs.jpcb.0c0961110.1021/acs.jpcb.0c09611 ↗
46. 10.1021/bc990030v10.1021/bc990030v ↗
47. 10.1093/nar/23.5.75310.1093/nar/23.5.753 ↗
48. 10.1023/B:JOFL.0000016290.34070.ee10.1023/B:JOFL.0000016290.34070.ee ↗
49. 10.1016/j.jlumin.2018.08.07410.1016/j.jlumin.2018.08.074 ↗
50. 10.1039/C8SC05770B10.1039/C8SC05770B ↗
51. 10.1039/C5OB00675A10.1039/C5OB00675A ↗
52. 10.1021/jp980836c10.1021/jp980836c ↗
53. 10.1038/nrd103310.1038/nrd1033 ↗
54. 10.1021/acsomega.5c1014210.1021/acsomega.5c10142 ↗
55. 10.1039/C4AN01912A10.1039/C4AN01912A ↗
56. 10.1039/c1cp20812h10.1039/c1cp20812h ↗
57. 10.1016/j.chemphys.2021.11139210.1016/j.chemphys.2021.111392 ↗
58. 10.1002/(SICI)1096-987X(199604)17:5/6<587::AID-JCC4>3.0.CO;2-Q10.1002/(SICI)1096-987X(199604)17:5/6<587::AID-JCC4>3.0.CO;2-Q ↗
59. 10.1039/C4CC01147C10.1039/C4CC01147C ↗
60. 10.1021/acsomega.7b0089910.1021/acsomega.7b00899 ↗
61. 10.1021/acs.bioconjchem.9b0065510.1021/acs.bioconjchem.9b00655 ↗
62. 10.1016/j.biochi.2024.06.00910.1016/j.biochi.2024.06.009 ↗
63. 10.1039/c0cc00956c10.1039/c0cc00956c ↗
64. 10.1021/cb500859710.1021/cb5008597 ↗
65. 10.1021/acsptsci.2c0020510.1021/acsptsci.2c00205 ↗
66. 10.1016/j.ejmech.2020.11220210.1016/j.ejmech.2020.112202 ↗
67. 10.1016/j.bbrc.2003.09.05210.1016/j.bbrc.2003.09.052 ↗
68. 10.1517/17460441.2015.103293610.1517/17460441.2015.1032936 ↗
69. 10.1021/bi00127a01010.1021/bi00127a010 ↗
70. 10.1017/S003358351000018110.1017/S0033583510000181 ↗
71. 10.1074/jbc.M116.71847810.1074/jbc.M116.718478 ↗
72. 10.1021/acsami.8b0786910.1021/acsami.8b07869 ↗