Synthesis, characterization and in-vitro studies of strontium-zinc co-substituted fluorohydroxyapatite for biomedical applications

Abstract The present study is to investigate the contribution of Sr2+ and Zn2+, two biologically important ions substituted into the structure of fluorohydroxyapatite (Ca10(PO4)6F0.5(OH)1.5, FHA). Fluorohydroxyapatite (FHA), a partial substitution of fluorine ion in HA has received a lot of interest as it improves the mechanical properties of orthopeadic implants. FHA samples co-substituted with Sr2+ and Zn2+ [xSr/5Zn-FHA, where x = 5–40 mol% of Sr2+ and 40Sr/yZn-FHA, where y = 5–20 mol% of Zn2+] is prepared by sol-gel method. The shift in FTIR and XRD peaks observed after the co-substitution of Sr2+ and Zn2+ ions confirmed the doping. Higher Zn2+ concentration decreases the crystallite size and aids in forming β-TCP phase. Co-substitution of Sr2+ and Zn2+ increases the microhardness and fracture toughness, while microstructural analysis reveals the decrease in grain size. In-vitro studies are conducted on the samples by immersing in SBF solution for 14 days to investigate the osteogenic bone forming ability. The FESEM results reveal the formation of apatite layers on the surface which proves the osteoconductive nature.