Collagen/fibrin microbeads as a delivery system for Ag-doped bioactive glass and DPSCs for potential applications in dentistry
Source abstract
Abstract
Abstract Silver doped bioactive glass (Ag-BG) and Dental Pulp Stem Cells (DPSCs) have shown promising properties for dental tissue regeneration. This study presents the fabrication and characterization of composite collagen/fibrin microbeads that aimed to incorporate and successfully deliver both Ag-BG and DPSC. Cell viability, cell differentiation in vitro and in vivo, and antibacterial properties of the systems against Escherichia coli ( E. coli ), Streptococcus mutans ( S. mutans ) and Enterococcus faecalis ( E. faecalis ) were evaluated. Cell viability and proliferation of DPSCs within the delivery system were found unaltered in both combinations (Beads/DPSCs and Beads/DPSCs/Ag-BG). Our results show that Ag-BG and DPSCs were successfully incorporated and proliferated within the collagen/fibrin microbeads. DPSCs continued to proliferate up to 6-weeks in cell culture but cell differentiation into odontoblast-like cells was observed only in vivo. The beads containing Ag-BG strongly inhibit bacterial growth in the experimental groups (beads alone, Beads/Ag-BG and Beads/DPSCs/Ag-BG). Our data suggests that collagen/fibrin microbead paste could have clinical applicability in the regenerative dentistry field.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Periodontal Regeneration and Treatments · Bone Tissue Engineering Materials · Dental Implant Techniques and Outcomes
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (46)
Show source-provided references
1. Scaffolds based bone tissue engineering: the role of chitosan10.1089/ten.teb.2010.0704 ↗
2. Polymer-based composite scaffolds for tissue engineering
3. Improving viability of stem cells during syringe needle flow through the design of hydrogel cell carriers10.1089/ten.tea.2011.0391 ↗
4. Collagen–chitosan polymer as a scaffold for the proliferation of human adipose tissue-derived stem cells10.1007/s10856-008-3636-6 ↗
5. Confined 3D microenvironment regulates early differentiation in human pluripotent stem cells10.1002/bit.24571 ↗
6. Controlling stem cell fate with material design10.1002/adma.200901055 ↗
7. Responsive micromolds for sequential patterning of hydrogel microstructures10.1021/ja204266a ↗
8. Comparison of uncultured marrow mononuclear cells and culture-expanded mesenchymal stem cells in 3D collagen-chitosan microbeads for orthopedic tissue engineering10.1089/ten.tea.2013.0151 ↗
9. Encapsulation of adult human mesenchymal stem cells within collagen–agarose microenvironments10.1002/bit.20614 ↗
10. Mesenchymal stem cell-encapsulated collagen microspheres for bone tissue engineering10.1089/ten.tec.2008.0709 ↗
11. Self-assembly and tissue fusion of toroid-shaped minimal building units10.1089/ten.tea.2009.0607 ↗
12. Cell encapsulation in sub-mm sized gel modules using replica molding10.1371/journal.pone.0002258 ↗
13. Modular tissue engineering: engineering biological tissues from the bottom up10.1039/b814285h ↗
14. Osteogenic differentiation of mesenchymal stem cells in defined protein beads10.1002/jbm.b.31098 ↗
15. The natural and engineered 3D microenvironment as a regulatory cue during stem cell fate determination10.1089/ten.teb.2009.0270 ↗
16. Phase-separated chitosan–fibrin microbeads for cell delivery10.3109/02652048.2011.569764 ↗
17. Core-cross-linked alginate microcapsules for cell encapsulation10.1021/bm801330j ↗
18. Development of mammalian cell-enclosing subsieve-size agarose capsules (<100μm) for cell therapy10.1016/j.biomaterials.2004.11.043 ↗
19. Culture of human mesenchymal stem cells using electrosprayed porous chitosan microbeads10.1002/jbm.a.32417 ↗
20. Dual-phase osteogenic and vasculogenic engineered tissue for bone formation10.1089/ten.tea.2013.0740 ↗
21. The use of human mesenchymal stem cells encapsulated in RGD modified alginate microspheres in the repair of myocardial infarction in the rat10.1016/j.biomaterials.2010.05.078 ↗
22. Fibrin structure and wound healing10.1111/j.1538-7836.2006.01861.x ↗
23. Fibrinogen and fibrin structure and functions10.1111/j.1538-7836.2005.01365.x ↗
24. Fibrin as a delivery system for therapeutic drugs and biomolecules10.1089/ten.teb.2008.0527 ↗
25. 2D and 3D collagen and fibrin biopolymers promote specific ECM and integrin gene expression by vascular smooth muscle cells10.1163/156856208786052380 ↗
26. Properties of engineered vascular constructs made from collagen, fibrin, and collagen–fibrin mixtures10.1016/j.biomaterials.2003.10.073 ↗
27. Self-assembled collagen–human mesenchymal stem cell microspheres for regenerative medicine10.1016/j.biomaterials.2007.07.041 ↗
28. Biomaterials in tissue engineering10.1038/nbt0695-565 ↗
29. Initial investigation of novel human‐like collagen/chitosan scaffold for vascular tissue engineering10.1002/jbm.a.32256 ↗
30. Collagen/chitosan porous scaffolds with improved biostability for skin tissue engineering10.1016/S0142-9612(03)00374-0 ↗
31. Preparation and evaluation of porous chitosan/collagen scaffolds for periodontal tissue engineering10.1177/0883911506065100 ↗
32. Growth and differentiation of mouse osteoblasts on chitosan–collagen sponges10.1016/j.ijom.2006.09.023 ↗
33. Implantable applications of chitin and chitosan10.1016/S0142-9612(03)00026-7 ↗
34. Development of new sol–gel derived Ag-doped biomaterials for dental applications
35. Fabrication and characterization of bioactive and antibacterial composites for dental applications10.1016/j.actbio.2014.04.030 ↗
36. Winner for outstanding research in the Ph. D. category for the 2013 society for biomaterials meeting and exposition, April 10–13, 2013, Boston, Massachusetts10.1002/jbm.a.34611 ↗
37. Noninvasive, quantitative, spatiotemporal characterization of mineralization in three-dimensional collagen hydrogels using high-resolution spectral ultrasound imaging10.1089/ten.tec.2012.0180 ↗
38. Sol–gel based fabrication of novel glass-ceramics and composites for dental applications10.1016/j.msec.2010.03.005 ↗
39. Ag-doped sol–gel derived novel composite materials for dental applications
40. Development of a serum‐free system to expand dental‐derived stem cells: PDLSCs and SHEDs10.1002/jcp.22304 ↗
41. Dental pulp tissue engineering with stem cells from exfoliated deciduous teeth10.1016/j.joen.2008.04.009 ↗
42. Bioglass® 45S5 stimulates osteoblast turnover and enhances bone formation in vitro: implications and applications for bone tissue engineering10.1007/s002230001134 ↗
43. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo10.1073/pnas.240309797 ↗
44. Three-dimensional mineralization of dense nanofibrillar collagen–bioglass hybrid scaffolds10.1021/bm1001087 ↗
45. Novel collagen scaffolds with predefined internal morphology made by solid freeform fabrication10.1016/S0142-9612(02)00528-8 ↗
46. Antimicrobial macroporous gel-glasses: dissolution and cytotoxicity