Detection and analysis of human serum albumin nanoparticles within phagocytic cells at the resolution of individual live cell or single 3D multicellular spheroid
Source abstract
Abstract
Since nanoparticles (NPs) have shown great potential in various biomedical applications, live cell response to NPs should be thoroughly explored prior to their in vivo use. In the current study, live cell array (LCA) methodology and unique cell-based assays were used to study the interaction of magnetite (HSA-Mag NP) loaded human serum albumin NPs with phagocytic cells. The LCA enabled cell culturing during HSA-Mag NP accumulation and monolayer or spheroid formation, concomitantly with on-line monitoring of NP internalization. These platforms were also utilized for imaging intercellular links between living cells preloaded with HSA-Mag NP in 2D and 3D resolution. HSA-Mag NP uptake by cells was quantified by imaging, and analyzed using time-resolved measurements. Image analysis of the individual cells in cell populations showed accumulation of HSA-Mag NP by promonocytes and glial cells in a dose- and time-dependent manner. High variability of NP accumulation in individual cells within cell populations, as well as cell subgroups, was evident in both cell types. Following 24 h interaction, uptake of HSA-Mag NP was about 10 times more efficient in glial cells than in activated promonocytes. The presented assays may facilitate detection and analysis of the amount of NPs within individual cells, as well as the rate of NP accumulation and processing in different subsets of living cells. Such data are crucial for estimating predicted drug dosage delivered by NPs, as well as to study possible mechanisms for NP interference with live cells.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanoparticle-Based Drug Delivery · Microfluidic and Bio-sensing Technologies · 3D Printing in Biomedical Research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (31)
Show source-provided references
1. Blechinger J, Bauer AT, Torrano AA, Gorzelanny C, Bräuchle C, Schneider SW (2013) Uptake kinetics and nanotoxicity of silica nanoparticles are cell type dependent. Small. doi: 10.1002/smll.201301004
2. Dawidczyk CM, Russell LM, Searson PC (2014) Nanomedicines for cancer therapy: state-of-the-art and limitations to pre-clinical studies that hinder future developments. Front Chem 2:69. doi: 10.3389/fchem.2014.0006910.3389/fchem.2014.00069 ↗
3. Deutsch M, Deutsch A, Shirihai O, Hurevich I, Afrimzon E, Shafran Y, Zurgil N (2006) A novel miniature cell retainer for correlative high-content analysis of individual untethered non-adherent cells. Lab Chip 6:995–100010.1039/b603961h ↗
4. Eustaquio T, Leary JF (2012) Single-cell nanotoxicity assays of superparamagnetic iron oxide nanoparticles. In: Reineke J (ed) Nanotoxicity: methods and protocols. Methods Mol Biol 926:69–85. Humana Press10.1007/978-1-62703-002-1_5 ↗
5. Gao J, Zhang W, Huang P, Zhang B, Zhang X, Xu B (2008) Intracellular spatial control of fluorescent magnetic nanoparticles. J Am Chem Soc 130:3710–371110.1021/ja7103125 ↗
6. Horie M, Kato H, Fujita K, Endoh S, Iwahashi H (2012) In vitro evaluation of cellular response induced by manufactured nanoparticles. Chem Res Toxicol 25(3):605–61910.1021/tx200470e ↗
7. Hoysentruyt LC, Akgos Z, Seyfried TN (2011) Hypothesis: are neoplastic macrophages/microglia present in glioblastoma multiforme? ASN Neuro 3(4):e00064. doi: 10.1042/AN20110011
8. Ibuki Y, Toyooka T (2012) Nanoparticle uptake measured by flow cytometry. In: Reineke J (ed) Nanotoxicity: methods and protocols, Methods Mol Biol 926:157–166. Humana Press, ISBN: 978-1-62703-001-4 (Print) 978-1-62703-002-1 (Online)10.1007/978-1-62703-002-1_11 ↗
9. Kettiger H, Schipanski A, Wick P, Huwyler J (2013) Engineered nanomaterial uptake and tissue distribution: from cell to organism. Int J Nanomed 8:3255–3269
10. Kruttwig K, Brueggemann C, Kaijzel E, Vorhagen S, Hilger T, Löwik C, Hoehn M (2009) Development of a three-dimensional in vitro model for longitudinal observation of cell behavior: monitoring by magnetic resonance imaging and optical imaging. Mol Imaging Biol. doi: 10.1007/s11307-009-0289-x
11. Langer K, Balthasar S, Vogel V, Dinauer N, von Briesen H, Schubert D (2003) Optimization of the preparation process for human serum albumin (HSA) nanoparticles. Int J Pharm 257:169–18010.1016/S0378-5173(03)00134-0 ↗
12. Laurent S, Burtea C, Thirifays C, Häfeli UO, Mahmoudi M (2012) Crucial ignored parameters on nanotoxicology: the importance of toxicity assay modifications and “cell vision”. PLoS One 7(1):e29997. doi: 10.1371/journal.pone.002999710.1371/journal.pone.0029997 ↗
13. Leßig J, Neu B, Glander HJ, Arnhold J, Reibetanz U (2010) Phagocytotic competence of differentiated U937 cells for colloidal drug delivery systems in immune cells. Inflammation 34(2):99–110. doi: 10.1007/s10753-010-9213-410.1007/s10753-010-9213-4 ↗
14. Love SA, Maurer-Jones MA, Thompson JW, Lin YS, Haynes CL (2012) Assessing nanoparticle toxicity. Annu Rev Anal Chem (Palo Alto Calif) 5:181–20510.1146/annurev-anchem-062011-143134 ↗
15. Lukianova-Hleb EY, Ren X, Constantinou PE, Danysh BP, Shenefelt DL, Carson DD, Farach-Carson MC, Kulchitsky VA, Wu X, Wagner DS, Lapotko DO (2012) Improved cellular specificity of plasmonic nanobubbles versus nanoparticles in heterogeneous cell systems. PLoS One 7(4):e3453710.1371/journal.pone.0034537 ↗
16. Luo Y, Wang C, Hossain M, Qiao Y, Ma L, An J, Su M (2012) Three-dimensional microtissue assay for high-throughput cytotoxicity of nanoparticles. Anal Chem 84(15):6731–673810.1021/ac301191j ↗
17. Maldonado CR, Salassa L, Gomez-Blanco N, Mareque-Rivas JC (2013) Nano-functionalization of metal complexes for molecular imaging and anticancer therapy. Coord Chem Rev 257(19):2668–268810.1016/j.ccr.2013.04.014 ↗
18. Mandarano G, Lodhia J, Eu P, Ferris NJ, Davidson R, Cowell SF (2010) Development and use of iron oxide nanoparticles (Part 2): the application of iron oxide contrast agents in MRI. Biomed Imaging Interv J 6(2):e13. doi: 10.2349/biij.6.2.e1310.2349/biij.6.2.e13 ↗
19. Markovitz-Bishitz Y, Tauber Y, Afrimzon E, Zurgil N, Sobolev M, Shafran Y, Deutsch A, Howitz S, Deutsch M (2010) A polymer microstructure array for the formation, culturing, and high throughput drug screening of breast cancer spheroids. Biomaterials 31(32):8436–844410.1016/j.biomaterials.2010.07.050 ↗
20. Martínez-Vera NP, Schmidt R, Langer K, Zlatev I, Wronski R, Auer E, Havas D, Windisch M, von Briesen H, Wagner S, Stab J, Deutsch M, Pietrzik C, Fazekas F, Ropele S (2014) Tracking of magnetite labeled nanoparticles in the rat brain using MRI. PLoS One 9(3):e9206810.1371/journal.pone.0092068 ↗
21. Martins S, Costa-Lima S, Carneiro T, Cordeiro-da-Silva A, Souto EB, Ferreira DC (2012) Solid lipid nanoparticles as intracellular drug transporters: an investigation of the uptake mechanism and pathway. Int J Pharm 430(1):216–22710.1016/j.ijpharm.2012.03.032 ↗
22. Meister S, Zlatev I, Stab J, Docter D, Baches S, Stauber RH, Deutsch M, Schmidt R, Ropele S, Windisch M, Langer K, Wagner S, von Briesen H, Weggen S, Pietrzik CU (2013) Nanoparticulate flurbiprofen reduces amyloid-β42 generation in an in vitro blood–brain barrier model. Alzheimers Res Ther 5(6):51. doi: 10.1186/alzrt22510.1186/alzrt225 ↗
23. Sahay G, Alakhova DY, Kabanov AV (2010) Endocytosis of nanomedicines. J Control Release 145(3):182–19510.1016/j.jconrel.2010.01.036 ↗
24. Sebak S, Mirzaei M, Malhotra M, Kulamarva A, Prakash S (2010) Human serum albumin nanoparticles as an efficient noscapine drug delivery system for potential use in breast cancer: preparation and in vitro analysis. Int J Nanomed 5:525–532
25. Singhal N, Handa U, Bansal C, Mohan H (2011) Neutrophil phagocytosis by tumor cells: a cytological study. Diagn Cytopathol 39:553–55510.1002/dc.21421 ↗
26. Sriraman SK, Aryasomayajula B, Torchilin VP (2014) Barriers to drug delivery in solid tumors. Tissue Barriers 2(3):e2952810.4161/tisb.29528 ↗
27. Wagner S, Zensi A, Wien SL, Tschickardt SE, Maier W, Vogel T, Worek F, Pietrzik CU, Kreuter J, von Briesen H (2012) Uptake mechanism of ApoE-modified nanoparticles on brain capillary endothelial cells as a blood-brain barrier model. PLoS One 7(3):e3256810.1371/journal.pone.0032568 ↗
28. Weber C, Kreuter J, Langer K (2000) Desolvation process and surface characteristics of HSA-nanoparticles. Int J Pharm 196(2):197–20010.1016/S0378-5173(99)00420-2 ↗
29. Yang Z, Liu ZW, Allaker RP, Reip P, Oxford J, Ahmad Z, Ren G (2010) A review of nanoparticle functionality and toxicity on the central nervous system. J R Soc Interface 7(Suppl 4):S411–S42210.1098/rsif.2010.0158.focus ↗
30. Zhu M, Nie G, Meng H, Xia T, Nel A, Zhao Y (2013) Physicochemical properties determine nanomaterial cellular uptake, transport, and fate. Acc Chem Res 46(3):622–63110.1021/ar300031y ↗
31. Zurgil N, Afrimzon E, Deutsch A, Namer Y, Shafran Y, Sobolev M, Tauber Y, Ravid-Hermesh O, Deutsch M (2010) Polymer live-cell array for real-time kinetic imaging of immune cells. Biomaterials 31(18):5022–502910.1016/j.biomaterials.2010.02.035 ↗