Using fluorescence measurement of zinc ions liberated from ZnS nanoparticle labels in bioassay for Escherichia coli O157:H7
Source abstract
Abstract
In this study, an alternative approach using ZnS nanoparticle biolabels as fluorescence signal transducers is reported for the immunoassay of E. coli O157:H7 in tap water samples. Instead of measuring the fluorescence of ZnS nanoparticles in the assay, the fluorescence signal is generated through the binding of zinc ions released from nanoparticle labels with zinc-ion sensitive fluorescence indicator Fluozin-3. In the assay, ZnS nanoparticles around 50 nm in diameter were synthesized, bioconjugated, and applied for the detection of E. coli O157:H7. The assay shows a detection range over two orders of magnitude and a detection limit around 1000 colony-forming units (cfu) of E. coli O157:H7.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Biosensors and Analytical Detection · Advanced biosensing and bioanalysis techniques · SARS-CoV-2 detection and testing
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (15)
Show source-provided references
1. Boyaci IH, Aguilar ZP, Hossain M, Halsall HB, Seliskar CJ, Heineman WR (2005) Amperometric determination of live Escherichia coli using antibody-coated paramagnetic beads. Anal Bioanal Chem 382:1234–124110.1007/s00216-005-3263-8 ↗
2. Fang X, Zhai T, Gautam UK, Li L, Wu L, Bando Y, Golberg D (2011) ZnS nanostructures: from synthesis to application. Prog Mat Sci 56:175–28710.1016/j.pmatsci.2010.10.001 ↗
3. Gee KR, Zhou Z-L, Qian W-J, Kennedy R (2001) Detection and imaging of zinc secretion from pancreatic β-cells using a new fluorescent zinc indicator. J Am Chem Soc 124:776–77810.1021/ja011774y ↗
4. Li Y, Chen J, Zhu C, Wang L, Zhao D, Zhuo S, Wu Y (2004) Preparation and application of cysteine-capped ZnS nanoparticles as fluorescence probe in the determination of nucleic acids. Spectrochim Acta A 60:1719–172410.1016/j.saa.2003.09.012 ↗
5. Li H, Shih W-Y, Shih W-H (2007) Non-heavy metal ZnS quantum dots with bright blue photoluminescence by a one-step aqueous synthesis. Nanotechnology 18:205604–20561010.1088/0957-4484/18/20/205604 ↗
6. Li L, Bian R, Ding Y, Yu M, Yu D (2009) Application of functionalized ZnS nanoparticles to determinate uracil and thymine as a fluorescence probe. Mater Chem Phys 113:905–90810.1016/j.matchemphys.2008.08.050 ↗
7. Liu G, Lin Y (2007) Nanomaterial labels in electrochemical immunosensors and immunoassays. Talanta 74:308–31710.1016/j.talanta.2007.10.014 ↗
8. Liu G, Wang J, Kim J, Jan MR, Collins GE (2004) Electrochemical coding for multiplexed immunoassays of proteins. Anal Chem 76:7126–713010.1021/ac049107l ↗
9. Pinwattana K, Wang J et al (2010) CdSe/ZnS quantum dots based electrochemical immunoassay for the detection of phosphorylated bovine serum albumin. Biosens Bioelectron 28:1109–111310.1016/j.bios.2010.08.021 ↗
10. Poitras C, Tufenkji N (2009) A QCM-D-based biosensor for E. coli O157:H7 highlighting the relevance of the dissipation slope as a transduction signal. Biosens Bioelectron 24:2137–214210.1016/j.bios.2008.11.016 ↗
11. Subramanian A, Irudayaraj J, Ryan T (2006) A mixed self-assembled monolayer-based surface plasmon immunosensor for detection of E. coli O157:H7. Biosens Bioelectron 21:998–100610.1016/j.bios.2005.03.007 ↗
12. Taylor AD, Yu Q, Chen S, Homola J, Jiang S (2005) Comparison of E. coli O157:H7 preparation methods used for detection with surface plasmon resonance sensor. Sensor Actuat B 107:202–20810.1016/j.snb.2004.11.097 ↗
13. Thomas JH, Kim SK, Hesketh PJ, Halsall HB, Heineman WR (2004) Bead-based electrochemical immunoassay for bacteriophage MS2. Anal Chem 76:2700–270710.1021/ac035503c ↗
14. Wu P, He Y, Wang H, Yan X (2010) Conjugation of glucose oxidase onto Mn-doped ZnS quantum dots for phosphorescent sensing of glucose in biological fluids. Anal Chem 82:1427–143310.1021/ac902531g ↗
15. Zhu N, Zhang A, He P, Fang Y (2004) DNA hybridization at magnetic nanoparticles with electrochemical strip detection. Electroanalysis 16:1925–193010.1002/elan.200303028 ↗