Hyaluronan functionalizing QDs as turn-on fluorescent probe for targeted recognition CD44 receptor
Source abstract
Abstract
The recognition of tumor markers in living cancer cells has attracted increasing interest. In the present study, the turn-on fluorescence probe was designed based on the fluorescence of thiolated chitosan-coated CdTe QDs (CdTe/TCS QDs) quenched by hyaluronan, which could provide the low background signal for sensitive cellular imaging. This system is expected to offer specific recognition of CD44 receptor over other substances owing to the specific affinity of hyaluronan and CD44 receptor (~8–9 kcal/mol). The probe is stable in aqueous and has little toxicity to living cells; thus, it can be utilized for targeted cancer cell imaging. The living lung cancer cell imaging experiments further demonstrate its value in recognizing cell-surface CD44 receptor with turn-on mode. In addition, the probe can be used to recognize and differentiate the subtypes of lung cancer cells based on the difference of CD44 expression on the surface of lung cancer cells. And, the western blot test further confirmed that the expression level of the CD44 receptor in lung cancer cells is different. Therefore, this probe may be potentially applied in recognizing lung cancer cells with higher contrast and sensitivity and provide new tools for cancer prognosis and therapy.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanoplatforms for cancer theranostics · Proteoglycans and glycosaminoglycans research · Advanced biosensing and bioanalysis techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (38)
Show source-provided references
1. Adams S, Zhang JZ (2016) Unique optical properties and applications of hollow gold nanospheres (HGNs). Coord Chem Rev 320–321:18–3710.1016/j.ccr.2016.01.014 ↗
2. Aydanur Kargl H, Fatih Kuyucuo-lua M, Mehmet Alakavukla P, Akpmar O, Erk S (1997) CD44 expression in metastatic and non-metastatic non-small cell lung cancers. Cancer Lett 119:27–3010.1016/S0304-3835(97)00254-1 ↗
3. Bao HF, Wang EK, Dong SJ (2006) One-pot synthesis of CdTe nanocrystals and shape control of luminescent CdTe-cystine nanocomposites. Small 2:476–48010.1002/smll.200500346 ↗
4. Bartolazzi A, Peach R, Aruffo A, Stamenkovic I (1994) Interaction between CD44 and hyaluronate is directly implicated in the regulation of tumor-development. J Exp Med 180:53–6610.1084/jem.180.1.53 ↗
5. Bianying F, Linjie G, Lihua W, Fan L, Jianxin L, Jimin G, Chunhai F, Qing H (2013) A graphene oxide-based fluorescent biosensor for the analysis of peptide-receptor interactions and imaging in somatostatin receptor subtype 2 overexpressed tumor cells. Anal Chem 85:7732–773710.1021/ac4009463 ↗
6. Chiu S-H, Gedda G, Girma WM, Chen J-K, Ling Y-C, Ghule AV, Ou K-L, Chang J-Y (2016) Rapid fabrication of carbon quantum dots as multifunctional nanovehicles for dual-modal targeted imaging and chemotherapy. Acta Biomater 46:151–16410.1016/j.actbio.2016.09.027 ↗
7. De Crozals G, Bonnet R, Farre C, Chaix C (2016) Nanoparticles with multiple properties for biomedical applications: a strategic guide. Nano Today 11:435–46310.1016/j.nantod.2016.07.002 ↗
8. Ghiaci M, Dorostkar N, Victoria Martinez-Huerta M, Fierro JLG, Moshiri P (2013) Synthesis and characterization of gold nanoparticles supported on thiol functionalized chitosan for solvent-free oxidation of cyclohexene with molecular oxygen. J Mol Catal A Chem 379:340–34910.1016/j.molcata.2013.08.030 ↗
9. Guo A, Zhu R, Ren Y, Dong J, Feng L (2016) A “turn-on” fluorescent chemosensor for aluminum ion and cell imaging application. Spectrochim Acta A Mol Biomol Spectrosc 153:530–53410.1016/j.saa.2015.09.009 ↗
10. Guvench O (2015) Revealing the mechanisms of protein disorder and N-glycosylation in CD44-hyaluronan binding using molecular simulation. Front Immunol 6:1–910.3389/fimmu.2015.00305 ↗
11. Kim HK, Quan YH, Lim JY, Choi BH, Park JH, Choi YH (2017) P2.01-013 HA-liposome Nanocarrier containing CD44 siRNA as a targeted chemotherapy to CD44 related chemoresistant non-small cell lung cancer: topic: analysis of RNA. J Thorac Oncol, 12:S79110.1016/j.jtho.2016.11.1065 ↗
12. Kondo K, Miyoshi T, Hino N, Shimizu E, Masuda N, Takada M, Uyama T, Monden Y (1998) High frequency expressions of CD44 standard and variant forms in non-small cell lung cancers, but not in small cell lung cancers. J Surg Oncol 69:128–13610.1002/(SICI)1096-9098(199811)69:3<128::AID-JSO3>3.0.CO;2-M ↗
13. Leung EL, Fiscus RR, Tung JW, Tin VP, Cheng LC, Sihoe AD, Fink LM, Ma Y, Wong MP.(2010) Non-small cell lung cancer cells expressing CD44 are enriched for stem cell-like properties. PLoS ONE. 1110.1371/journal.pone.0014062 ↗
14. Li X-P, Zhang X-W, Zheng L-Z, Guo W-J (2015) Expression of CD44 in pancreatic cancer and its significance. Int J Clin Exp Pathol 8:6724–6731
15. Liu Q, Xu S, Niu C, Li M, He D, Lu Z, Ma L, Na N, Huang F, Jiang H, Ouyang J (2015) Distinguish cancer cells based on targeting turn-on fluorescence imaging by folate functionalized green emitting carbon dots. Biosens Bioelectron 64:119–12510.1016/j.bios.2014.08.052 ↗
16. Loeffler-Ragg J, Germing U, Sperr WR, Herrmann H, Zwierzina H, Valent P, Ulmer H, Stauder R (2011) Serum CD44 levels predict survival in patients with low-risk myelodysplastic syndromes. Crit Rev Oncol Hematol 78:150–16110.1016/j.critrevonc.2010.05.008 ↗
17. Luo Z, Wu RR, Lv L, Li P, Zhang LY, Hao QL, Li W (2014) Prognostic value of CD44 expression in non-small cell lung cancer: a systematic review. Int J Clin Exp Pathol 7:3632–3646
18. Malvezzi M, Carioli G, Bertuccio P, Boffetta P, Levi F, La Vecchia C, Negri E (2017) European cancer mortality predictions for the year 2017, with focus on lung cancer. Ann Oncol Off J Eur Soc Med Oncol 28:1117–112310.1093/annonc/mdx033 ↗
19. Miao Y, Zhang Z, Gong Y, Zhang Q, Yan G (2014) Self-assembly of manganese doped zinc sulfide quantum dots/CTAB nanohybrids for detection of rutin. Biosens Bioelectron 52:271–27610.1016/j.bios.2013.08.011 ↗
20. Misra S, Heldin P, Hascall VC, Karamanos NK, Skandalis SS, Markwald RR, Ghatak S (2011) Hyaluronan-CD44 interactions as potential targets for cancer therapy. FEBS J 278:1429–144310.1111/j.1742-4658.2011.08071.x ↗
21. Namdari P, Negahdari B, Eatemadi A (2017) Synthesis, properties and biomedical applications of carbon-based quantum dots: an updated review. Biomed Pharmacother 87:209–22210.1016/j.biopha.2016.12.108 ↗
22. Ohashi R, Takahashi F, Cui R, Yoshioka M, Gu T, Sasaki S, Tominaga S, Nishio K, Tanabe KK, Takahashi K (2007) Interaction between CD44 and hyaluronate induces chemoresistance in non-small cell lung cancer cell. Cancer Lett 252:225–23410.1016/j.canlet.2006.12.025 ↗
23. Pitule P, Cedikova M, Daum O, Vojtisek J, Vycital O, Hosek P, Treska V, Hes O, Kralickova M, Liska V (2014) Immunohistochemical detection of cancer stem cell related markers CD44 and CD133 in metastatic colorectal cancer patients. Biomed Res Int 2014:432139–43213910.1155/2014/432139 ↗
24. Prabaharan M, Gong S (2008) Novel thiolated carboxymethyl chitosan-g-beta-cyclodextrin as mucoadhesive hydrophobic drug delivery carriers. Carbohydr Polym 73:117–12510.1016/j.carbpol.2007.11.005 ↗
25. Quan YH, Kim B, Park J-H, Choi Y, Choi YH, Kim HK (2014) Highly sensitive and selective anticancer effect by conjugated HA-cisplatin in non-small cell lung cancer overexpressed with CD44. Exp Lung Res 40:475–48410.3109/01902148.2014.905656 ↗
26. Reddy C, Chilla D, Boltax J (2011) Lung cancer screening: a review of available data and current guidelines. Hosp Pract 39:107–11210.3810/hp.2011.10.929 ↗
27. Salgado CL, Mansur AAP, Mansur HS, Monteiro FJM (2014) Fluorescent bionanoprobes based on quantum dot-chitosan-O-phospho-L-serine conjugates for labeling human bone marrow stromal cells. RSC Adv 4:49016–4902710.1039/C4RA08247H ↗
28. Shi W, Ma H (2012) Spectroscopic probes with changeable pi-conjugated systems. Chem Commun 48:8732–874410.1039/c2cc33366j ↗
29. Tian F, Yang J, Huang W, Zhou S, Yao G (2013) Resonance Rayleigh scattering, frequency doubling scattering and absorption spectrum of the interaction for mebendazole with 12-tungstophosphoric acid and its analytical applications. Spectrochim Acta A Mol Biomol Spectrosc 116:57–6410.1016/j.saa.2013.06.018 ↗
30. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A (2015) Global cancer statistics, 2012. CA Cancer J Clin 65:87–10810.3322/caac.21262 ↗
31. Wan J, Xing L, Wang W (2014) Preparation of a lead sensor based on porous multiwalled carbon nanotubes/thiolated chitosan composite materials. Turk J Chem 38:182–18810.3906/kim-1212-33 ↗
32. Wang SM, Huang QZ, Wang QS (2005) Study on the synergetic degradation of chitosan with ultraviolet light and hydrogen peroxide. Carbohydr Res 340:1143–114710.1016/j.carres.2005.02.009 ↗
33. Wang C-H, Chang C-W, Peng C-A (2011) Gold nanorod stabilized by thiolated chitosan as photothermal absorber for cancer cell treatment. J Nanopart Res 13:2749–275810.1007/s11051-010-0162-5 ↗
34. Wang Y, Liu Y, Xiao B (2015) Rapid and reliable detection of CD44 variants in gastric carcinoma using a nested reverse transcription-polymerase chain reaction. Oncol Lett 10:2962–296610.3892/ol.2015.3664 ↗
35. Xia Z, Wu S, Chen J (2013) Preparation of water soluble chitosan by hydrolysis using hydrogen peroxide. Int J Biol Macromol 59:242–24510.1016/j.ijbiomac.2013.04.034 ↗
36. Yang L, Li X, Qu Y, Qu W, Zhang X, Hang Y, Agren H, Hua J (2014) Red turn-on fluorescent phenazine-cyanine chemodosimeters for cyanide anion in aqueous solution and its application for cell imaging. Sensors Actuators B Chem 203:833–84710.1016/j.snb.2014.07.045 ↗
37. Yukawa H, Baba Y (2017) In vivo fluorescence imaging and the diagnosis of stem cells using quantum dots for regenerative medicine. Anal Chem 89:2671–268110.1021/acs.analchem.6b04763 ↗
38. Zhang Y, Yang D, Weng L, Wang L (2013) Early lung cancer diagnosis by biosensors. Int J Mol Sci 14:15479–1550910.3390/ijms140815479 ↗