Interaction between protoporphyrin IX and tryptophan silver nanoparticles
Source abstract
Abstract
Silver nanoparticles (AgNPs) have been intensively studied for several purposes including therapeutic applications in cancer. When prepared with tryptophan and photoreduction, silver nanoparticles (TrpAgNPs) become an alternative to conventional anticancer drugs. In this study, the anticancer activity of synthesized TrpAgNPs against MCF-7 breast cancer cells was evaluated, and the inhibitory concentration (IC50) was found to be ~3.4 mg/mL. Since the protoporphyrin IX (PPIX) concentrations in tumor cells are elevate compared to normal cells, the PPIX-TrpAgNP interaction was studied to investigate if it could contribute for cell apoptosis. The investigation was performed using PPIX solution (0.9 μg/mL) with different TrpAgNP concentrations (from 0 to 13 mg/mL). PPIX was characterized by UV-Vis spectroscopy, steady-state and time-resolved fluorescence spectroscopy. The results have shown that the presence of spherical TrpAgNps with 16-nm diameter quench the PPIX fluorescence intensity. This quenching is strongly dependent on the concentration of the TrpAgNPs, and it is caused by a combination of a static and a dynamic process. The chemical binding leads to oxidation of tryptophan and formation of kynurenine, observed in the emission spectra around 470 nm. The strong reduction of the PPIX fluorescence decay lifetime with nanoparticle increasing concentration confirms the quenching processes due to charge transfer from the excited PPIX states to the resonant silver states. The present study confirms the anticancer activity of TrpAgNPs on the human breast cancer cell line (MCF-7) in vitro and indicates that PPIX-AgNP interaction could contribute with MCF-7 apoptosis.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanoparticles: synthesis and applications · Porphyrin and Phthalocyanine Chemistry · Nanoparticle-Based Drug Delivery
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (52)
Show source-provided references
1. Austin LA, Ahmad S, Kang B, Rommel KR, Mahmoud M, Peek ME, El-Sayed MA (2015) Cytotoxic effects of cytoplasmic-targeted and nuclear-targeted gold and silver nanoparticles in HSC-3 cells - a mechanistic study. Toxicol in Vitro 29:694–70510.1016/j.tiv.2014.11.003 ↗
2. Bae SJ, Lee DS, Berezin V, Kang U, Lee KH (2016) Multispectral autofluorescence imaging for detection of cervical lesions: a preclinical study. J Obstet Gynaecol Res 42:1846–185310.1111/jog.13101 ↗
3. Baskaran B, Muthukumarasamy A, Chidambaram S, Sugumaran A, Ramachandran K, Manimuthu TR (2017) Cytotoxic potentials of biologically fabricated platinum nanoparticles from Streptomyces sp. on MCF-7 breast cancer cells. Iet Nanobiotechnol 11:241–24610.1049/iet-nbt.2016.0040 ↗
4. Bastus NG, Merkoci F, Piella J, Puntes V (2014) Synthesis of highly monodisperse citrate-stabilized silver nanoparticles of up to 200 nm: kinetic control and catalytic properties. Chem Mater 26:2836–284610.1021/cm500316k ↗
5. Blanco KC, Moriyama LT, Inada NM, Salvio AG, Menezes PFC, Leite EJS, Kurachi C, Bagnato VS (2015) Fluorescence guided PDT for optimization of the outcome of skin cancer treatment. Front Phys 3:3010.3389/fphy.2015.00030 ↗
6. Bravo JJ, Olson JD, Davis SC, Roberts DW, Paulsen KD, Kanick SC (2017) Hyperspectral data processing improves PpIX contrast during fluorescence guided surgery of human brain tumors. Sci Rep 7:945510.1038/s41598-017-09727-8 ↗
7. Courrol LC, Silva FR d O, Gomes L (2007) A simple method to synthesize silver nanoparticles by photo-reduction. Colloids Surf A Physicochem Eng Asp 305:54–5710.1016/j.colsurfa.2007.04.052 ↗
8. de Matos RA, Courrol LC (2017) Biocompatible silver nanoparticles prepared with amino acids and a green method. Amino Acids 49:379–38810.1007/s00726-016-2371-4 ↗
9. de Oliveira Silva FR, Bellini MH, Nabeshima CT, Schor N, Vieira ND Jr, Courrol LC (2011) Enhancement of blood porphyrin emission intensity with aminolevulinic acid administration: a new concept for photodynamic diagnosis of early prostate cancer. Photodiagn Photodyn Ther 8:7–1310.1016/j.pdpdt.2010.12.006 ↗
10. Desai R, Mankad V, Gupta SK, Jha PK (2012) Size distribution of silver nanoparticles: UV-visible spectroscopic assessment. Nanosci Nanotechnol Lett 4(4):30–3410.1166/nnl.2012.1278 ↗
11. Devi L, Das S, Mandal AB (2014) Impact of surface functionalization of AgNPs on binding and conformational change of hemoglobin ( Hb) and hemolytic behavior. J Phys Chem C 118:29739–2974910.1021/jp5075048 ↗
12. Durán M, Silveira CP, Durán N (2015) Catalytic role of traditional enzymes for biosynthesis of biogenic metallic nanoparticles: a mini-review. IET Nanobiotechnol 9:314–32310.1049/iet-nbt.2014.0054 ↗
13. Feliu N, Huhn J, Zyuzin MV, Ashraf S, Valdeperez D, Masood A, Said AH, Escudero A, Pelaz B, Gonzalez E, Duarte MAC, Roy S, Chakraborty I, Lim ML, Sjoqvist S, Jungebluth P, Parak WJ (2016) Quantitative uptake of colloidal particles by cell cultures. Sci Total Environ 568:819–82810.1016/j.scitotenv.2016.05.213 ↗
14. Feuerstein T, Berkovitch-Luria G, Nudelman A, Rephaeli A, Malik Z (2011) Modulating ALA-PDT efficacy of mutlidrug resistant MCF-7 breast cancer cells using ALA prodrug. Photochem Photobiol Sci 10:1926–193310.1039/c1pp05205e ↗
15. Giantsoudi D, Adams J, MacDonald SM, Paganetti H (2017) Proton treatment techniques for posterior Fossa tumors: consequences for linear energy transfer and dose-volume parameters for the brainstem and organs at risk. Int J Radiat Oncol Biol Phys 97:401–41010.1016/j.ijrobp.2016.09.042 ↗
16. Gottesman R, Shukla S, Perkas N, Solovyov LA, Nitzan Y, Gedanken A (2011) Sonochemical coating of paper by microbiocidal silver nanoparticles. Langmuir 27:720–72610.1021/la103401z ↗
17. Han Y and Chen J J Z 2013 Oxidative stress induces mitochondrial DNA damage and cytotoxicity through independent mechanisms in human cancer cells. Biomed Res Int10.1155/2013/825065 ↗
18. Horikoshi S, Abe H, Torigoe K, Abe M, Serpone N (2010) Access to small size distributions of nanoparticles by microwave-assisted synthesis. Formation of ag nanoparticles in aqueous carboxymethylcellulose solutions in batch and continuous-flow reactors. Nanoscale 2:1441–144710.1039/c0nr00141d ↗
19. Huntosova V, Gerelli E, Zellweger M, Wagnieres G (2016) Effect of PpIX photoproducts formation on pO(2) measurement by time-resolved delayed fluorescence spectroscopy of PpIX in solution and in vivo. J Photochem Photobiol B Biol 164:49–5610.1016/j.jphotobiol.2016.09.022 ↗
20. Imahori H, Fujimoto A, Kang S, Hotta H, Yoshida K, Umeyama T, Matano Y, Isoda S, Isosomppi M, Tkachenko NV, Lemmetyinen H (2005) Host– guest interactions in the supramolecular incorporation of fullerenes into tailored holes on porphyrin- modified gold nanoparticles in molecular photovoltaics. Chem Eur J 11:7265–727510.1002/chem.200500610 ↗
21. Kamat PV (2002) Photophysical, photochemical and photocatalytic aspects of metal nanoparticles. J Phys Chem B 106:7729–774410.1021/jp0209289 ↗
22. Kettler K, Veltman K, van de Meent D, van Wezel A, Hendriks AJ (2014) Cellular uptake of nanoparticles as determined by particle properties, experimental conditions, and cell type. Environ Toxicol Chem 33:481–49210.1002/etc.2470 ↗
23. Lesniak WG, Jyoti A, Mishra MK, Louissaint N, Romero R, Chugani DC, Kannan S, Kannan RM (2013) Concurrent quantification of tryptophan and its major metabolites. Anal Biochem 443:222–23110.1016/j.ab.2013.09.001 ↗
24. Li L, Chen Y, Wang XB, Feng XL, Wang P, Liu QH (2014) Comparison of protoporphyrin IX produced cell proliferation inhibition between human breast cancer MCF-7 and MDA-MB-231 cells. Pharmazie 69:621–628
25. Linnert T, Mulvaney P, Henglein A (1993) Surface-chemistry of colloidal silver - surface-plasmon damping by chemisorbed I-, SH-, And C6H5S. J Phys Chem 97:679–68210.1021/j100105a024 ↗
26. Mansfield JR, Gossage KW, Hoyt CC, Levenson RM (2005) Autofluorescence removal, multiplexing, and automated analysis methods for in-vivo fluorescence imaging. J Biomed Opt 10:04120710.1117/1.2032458 ↗
27. Millon SR, Ostrander JH, Yazdanfar S, Brown JQ, Bender JE, Rajeha A, Ramanujam N (2010) Preferential accumulation of 5-aminolevulinic acid-induced protoporphyrin IX in breast cancer: a comprehensive study on six breast cell lines with varying phenotypes. J Biomed Opt 15:01800210.1117/1.3302811 ↗
28. Mukha I, Vityuk N, Severynovska O, Eremenko A, Smirnova N (2016) The pH-dependent Stucture and properties of au and ag nanoparticles produced by tryptophan reduction. Nanoscale Res Lett 11:10110.1186/s11671-016-1318-8 ↗
29. Murphy S, Huang L, Kamat PV (2011) Charge- transfer complexation and excited- state interactions in porphyrin- silver nanoparticle hybrid structures. J Phys Chem C 115:22761–2276910.1021/jp205711x ↗
30. Namikawa T, Yatabe T, Inoue K, Shuin T, Hanazaki K (2015) Clinical applications of 5-aminolevulinic acid-mediated fluorescence for gastric cancer. World J Gastroenterol 21:8769–877510.3748/wjg.v21.i29.8769 ↗
31. Navarro JRG, Werts MHV (2013) Resonant light scattering spectroscopy of gold, silver and gold-silver alloy nanoparticles and optical detection in microfluidic channels. Analyst 138:583–59210.1039/C2AN36135C ↗
32. Rai MK, Deshmukh SD, Ingle AP, Gade AK (2012) Silver nanoparticles: the powerful nanoweapon against multidrug-resistant bacteria. J Appl Microbiol 112:841–85210.1111/j.1365-2672.2012.05253.x ↗
33. Sanna V, Pala N, Sechi M (2014) Targeted therapy using nanotechnology: focus on cancer. Int J Nanomedicine 9:467–483
34. Satapathy SR, Mohapatra P, Preet R, Das D, Sarkar B, Choudhuri T, Wyatt MD, Kundu CN (2013) Silver-based nanoparticles induce apoptosis in human colon cancer cells mediated through p53. Nanomedicine 8:1307–132210.2217/nnm.12.176 ↗
35. Satapathy SR, Mohapatra P, Preet R, Das D, Nayak A, Das S, Choudhuri T, Wyatt MD, Kundu CN (2014) Silver-based nanoparticles induce apoptosis in human colon cancer cells mediated through p53. Anticancer Res 34:6155–6156
36. Shang L, Nienhaus K, Nienhaus GU (2014) Engineered nanoparticles interacting with cells: size matters. J Nanobiotechnol 12:510.1186/1477-3155-12-5 ↗
37. Shmarakov IO, Mukha IP, Karavan VV, Chunikhin OY, Marchenko MM, Smirnova NP, Eremenko AM (2014) Tryptophan- assisted synthesis reduces bimetallic gold/ silver nanoparticle cytotoxicity and improves biological activity. Nanobiomedicine 1:610.5772/59684 ↗
38. Silva FRO, Nabeshima CT, Bellini MH, Schor N, Vieira ND Jr, Courrol LC (2013) Study of ProtoPorphyrin IX elimination by body excreta: a new noninvasive Cancer diagnostic method? J Fluoresc 23:131–13510.1007/s10895-012-1126-1 ↗
39. Song H, Park H, Kim YS, Kim KD, Lee HK, Cho DH, Yang JW, Hur DY (2011) L-kynurenine-induced apoptosis in human NK cells is mediated by reactive oxygen species. Int Immunopharmacol 11:932–93810.1016/j.intimp.2011.02.005 ↗
40. Sunar U, Rohrbach DJ, Morgan J, Zeitouni N, Henderson BW (2013) Quantification of PpIX concentration in basal cell carcinoma and squamous cell carcinoma models using spatial frequency domain imaging. Biomed Opt Express 4:531–53710.1364/BOE.4.000531 ↗
41. Suresh AK, Pelletier DA, Wang W, Morrell-Falvey JL, Gu BH, Doktycz MJ (2012) Cytotoxicity induced by engineered silver Nanocrystallites is dependent on surface coatings and cell types. Langmuir 28:2727–273510.1021/la2042058 ↗
42. Sweet MJ, Chesser A, Singleton I (2012) Review: metal-based nanoparticles; size, function, and areas for advancement in applied microbiology. Adv Appl Microbiol 80(80):113–14210.1016/B978-0-12-394381-1.00005-2 ↗
43. Tang Y, Shen YF, Huang LB, Lv GJ, Lei CH, Fan XY, Lin FX, Zhang YX, Wu LH, Yang YJ (2015) In vitro cytotoxicity of gold nanorods in A549 cells. Environ Toxicol Pharmacol 39:871–87810.1016/j.etap.2015.02.003 ↗
44. Thompson CM, Sonawane B, Barton HA, DeWoskin RS, Lipscomb JC, Schlosser P, Chiu WA, Krishnan K (2008) Approaches for applications of physiologically based pharmacokinetic models in risk assessment. J Toxicol Environ Health B Criti Rev 11:519–54710.1080/10937400701724337 ↗
45. Tomita RJ, de Matos RA, Vallim MA, Courrol LC (2014) A simple and effective method to synthesize fluorescent nanoparticles using tryptophan and light and their lethal effect against bacteria. J Photochem Photobiol B Biol 140:157–16210.1016/j.jphotobiol.2014.07.015 ↗
46. Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 160:1–4010.1016/j.cbi.2005.12.009 ↗
47. Weis BK, Balshawl D, Barr JR, Brown D, Ellisman M, Liov P, Omenn G, Potter JD, Smith MT, Sohn L, Suk WA, Sumner S, Swenberg J, Walt DR, Watkins S, Thompson C, Wilson SH (2005) Personalized exposure assessment: promising approaches for human environmental health research. Environ Health Perspect 113:840–84810.1289/ehp.7651 ↗
48. Xin LL, Wang JS, Fan GQ, Che BZ, Wu YH, Guo SF, Tong J (2016) Oxidative stress and mitochondrial injury-mediated cytotoxicity induced by silver nanoparticles in human A549 and HepG2 cells. Environ Toxicol 31:1691–169910.1002/tox.22171 ↗
49. Yang X, Palasuberniam P, Kraus D, Chen B (2015) Aminolevulinic acid-based tumor detection and therapy: molecular mechanisms and strategies for enhancement. Int J Mol Sci 16:25865–2588010.3390/ijms161025865 ↗
50. Yen H-J, Hsu S-h, Tsai C-L (2009) Cytotoxicity and immunological response of gold and silver nanoparticles of different sizes. Small 5:1553–156110.1002/smll.200900126 ↗
51. Zhang XF, Liu ZG, Shen W, Gurunathan S (2016) Silver nanoparticles: synthesis, characterization, properties, applications, and therapeutic approaches. Int J Mol Sci 17(34)10.3390/ijms17091534 ↗
52. Zhou YM, Liang XL, Dai ZF (2016) Porphyrin-loaded nanoparticles for cancer theranostics. Nanoscale 8:12394–1240510.1039/C5NR07849K ↗