Cooperative cytotoxic activity of Zn and Cu in bovine serum albumin-conjugated ZnS/CuS nano-composites in PC12 cancer cells
Source abstract
Abstract
Series of self-assembled and mono-dispersed bovine serum albumin (BSA)-conjugated ZnS/CuS nano-composites with different Zn/Cu ratios had been successfully synthesized by a combination method of the biomimetic synthesis and ion-exchange strategy under the gentle conditions. High-resolution transmission electron microscopy observation, Fourier transform infrared spectra and zeta potential analysis demonstrated that BSA-conjugated ZnS/CuS nano-composites with well dispersity had the hierarchical structure and BSA was a key factor to control the morphology and surface electro-negativity of final products. The real-time monitoring by atomic absorption spectroscopy and powder X-ray diffraction revealed that the Zn/Cu ratio of nano-composites could be controlled by adjusting the ion-exchange time. In addition, the metabolic and morphological assays indicated that the metabolic proliferation and spread of rat pheochromocytoma (PC12) cells could be inhibited by nano-composites, with the high anti-cancer activity at a low concentration (4 ppm). What were more important, Zn and Cu in nano-composites exhibited a positive cooperativity at inhibiting cancer cell functions. The microscope observation and biochemical marker analysis clearly revealed that the nano-composites-included lipid peroxidation and disintegration of membrane led to the death of PC12 cells. Summarily, the present study substantiated the potential of BSA-conjugated ZnS/CuS nano-composites as anti-cancer drug.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Advanced Nanomaterials in Catalysis · Nanoparticles: synthesis and applications · Quantum Dots Synthesis And Properties
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (21)
Show source-provided references
1. Biju V, Mundayoor S, Omkumar RV, Anas A, Ishikawa M (2010) Bioconjugated quantum dots for cancer research: present status, prospects and remaining issues. Biotechnol Adv 28:199–21310.1016/j.biotechadv.2009.11.007 ↗
2. Cao Y, Wang HJ, Cao C, Sun YY, Yang L, Wang BQ, Zhou JG (2011) Inhibition effects of protein-conjugated amorphous zinc sulfide nanoparticles on tumor cells growth. J Nanopart Res 13:2759–276710.1007/s11051-010-0163-4 ↗
3. Chimienti F, Jourdan E, Favier A, Seve M (2001) Zinc resistance impairs sensitivity to oxidative stress in HeLa cells: protection through metallothioneins expression. Free Radic Biol Med 31:1179–119010.1016/S0891-5849(01)00701-8 ↗
4. Cole AJ, Yang VC, David AE (2011) Cancer theranostics: the rise of targeted magnetic nanoparticles. Trends Biotechnol 29(7):323–33210.1016/j.tibtech.2011.03.001 ↗
5. Guo Y, Zhang J, Yang L, Wang H, Wang F, Zheng Z (2010a) Syntheses of amorphous and crystalline cupric sulfide nanoparticles and study on the specific activities on different cells. Chem Commun 46(20):3493–349510.1039/c001714k ↗
6. Guo WJ, Ye SS, Cao N, Huang J, Gao J, Chen QY (2010b) ROS-mediated autophagy was involved in cancer cell death induced by novel copper(II) complex. Exp Toxicol Pathol 62:577–58210.1016/j.etp.2009.08.001 ↗
7. Jamieson T, Bakhshi R, Petrova D, Pocock R, Imani M, Seifalian AM (2007) Biological applications of quantum dots. Biomaterials 28:4717–473210.1016/j.biomaterials.2007.07.014 ↗
8. Kawakami M, Inagawa R, Hosokawa T, Saito T, Kurasaki M (2008) Mechanism of apoptosis induced by copper in PC12 cells. Food Chem Toxicol 46:2157–216410.1016/j.fct.2008.02.014 ↗
9. Kerr SL, Sharp B (2007) Nano-particle labeling of nucleic acids for enhanced detection by inductively-coupled plasma mass spectrometry (ICP-MS). Chem Commun 21(43):4537–453910.1039/b709466c ↗
10. Limbach LK, Li Y, Grass RN, Brunner TJ, Hintermann MA, Muller M, Gunther D, Stark WJ (2005) Oxide nanoparticle uptake in human lung fibroblasts: effects of particle size, agglomeration, and diffusion at low concentrations. Environ Sci Technol 39(23):9370–937610.1021/es051043o ↗
11. Lorenz MR, Holzapfel V, Musyanovych A, Nothelfer K, Walther P, Frank H, Landfester K, Schrezenmeier H, Mailander V (2006) Uptake of functionalized, fluorescent-labeled polymeric particles in different cell lines and stem cells. Biomaterials 27(14):2820–282810.1016/j.biomaterials.2005.12.022 ↗
12. Min YK, Lee JE, Chung KC (2007) Zinc induces cell death in immortalized embryonic hippocampal cells via activation of Akt-GSK-3beta signaling. Exp Cell Res 313:312–32110.1016/j.yexcr.2006.10.013 ↗
13. Patil S, Sandberg A, Heckert E, Self W, Seal S (2007) Protein adsorption and cellular uptake of cerium oxide nanoparticles as a function of zeta potential. Biomaterials 28:4600–460710.1016/j.biomaterials.2007.07.029 ↗
14. Schroeder JE, Shweky I, Shmeeda H, Banin U, Gabizon A (2007) Folate-mediated tumor cell uptake of quantum dots entrapped in lipid nanoparticles. J Control Release 124:28–3410.1016/j.jconrel.2007.08.028 ↗
15. Sharm V, Shukla RK, Saxena N, Parmar D, Das M, Dhawan A (2009) DNA damaging potential of zinc oxide nanoparticles in human epidermal cells. Toxicol Lett 185:211–21810.1016/j.toxlet.2009.01.008 ↗
16. Shvedova AA, Pietroiusti A, Fadeel B, Kagan VE (2012) Mechanisms of carbon nanotube-induced toxicity: focus on oxidative stress. Toxicol Appl Pharmacol 261(2):121–13310.1016/j.taap.2012.03.023 ↗
17. Wang HJ, Yang L, Yang HY, Wang K, Yao WG, Jiang K, Huang XL, Zheng Z (2010) Antineoplastic activities of protein-conjugated silver sulfide nano-crystals with different shapes. J Inorg Biochem 104:87–9110.1016/j.jinorgbio.2009.10.015 ↗
18. Wang HJ, Yu XH, Cao Y, Zhou B, Wang CF (2012) Controllable synthesis and adjustable antineoplastic activity of bovine serum albumin-conjugated PbS/Ag2S core/shell nano-composites. J Inorg Biochem 113:40–4610.1016/j.jinorgbio.2012.04.004 ↗
19. Whaley SR, English DS, Hu EL, Barbara PF, Belcher AM (2000) Selection of peptides with semiconductor binding specificity for directed nanocrystal assembly. Nature 405:665–66810.1038/35015043 ↗
20. Wilhelm C, Billotey C, Roger J, Pons JN, Bacri JC, Gazeau F (2003) Intracellular uptake of anionic superparamagnetic nanoparticles as a function of their surface coating. Biomaterials 24(6):1001–101110.1016/S0142-9612(02)00440-4 ↗
21. Yang L, Wang HJ, Yang HY, Liu SH, Zhang BF, Wang K, Ma XM, Zheng Z (2008) Shape-controlled synthesis of protein-conjugated silver sulfide nanocrystals and study on the inhibition of tumor cell viability. Chem Commun 14(26):2995–299710.1039/b804274h ↗