Polymersomes as nanocarriers of vitamin D3: morphological and in vitro characterization
Source abstract
Abstract
Vitamin D exerts pleiotropic effects in the body, mainly in the mineral homeostasis as well as extra-skeletal actions. Deficiency of vitamin D is very frequent and its supplementation might be needed. In this work, a novel nanocarrier for vitamin D3 based on polymersomes is developed. The polymersomes were prepared by self-assembly of previously synthesized triblock copolymer including vitamin D3 or oil red, as model drug, and characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and dynamic light scattering (DLS), and its stability and entrapment efficiency were analyzed. The incorporation of the drugs produces an increase in the average size with a monomodal distribution of this, with high stability and entrapment efficiency. Vitamin D3 encapsulated in the polymersomes does not promote modifications at the hydrophilic-hydrophobic interface level as was shown by generalized polarization (GP) studies. In addition, cytotoxicity and biocompatibility studies using murine macrophage RAW 264.7 and bone marrow stem cells (BMSCs) show good biocompatibility without evidence of cytotoxicity under our tested conditions. Thus, the novel designed nanocarriers could be promising as delivery system for vitamin D3.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Vitamin D Research Studies · biodegradable polymer synthesis and properties · Polymer crystallization and properties
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (30)
Show source-provided references
1. Bikle DD (2000) Vitamin D: production, metabolism and mechanisms of action. 2021 In: Feingold KR, Anawalt B, Boyce A, Chrousos G, de Herder WW, Dhatariya K, Dungan K, Hershman JM, Hofland J, Kalra S, Kaltsas G, Koch C, Kopp P, Korbonits M, Kovacs CS, Kuohung W, Laferrère B, Levy M, McGee EA, McLachlan R, Morley JE, New M, Purnell J, Sahay R, Singer F, Sperling MA, Stratakis CA, Trence DL, Wilson DP, editors. Endotext [Internet]. South Dartmouth (MA): MDText.com, Inc
2. Janoušek J, Pilařová V, Macáková K, Nomura A, Veiga-Matos J, Silva DDD, Remião F, Saso L, Malá-Ládová K, Malý J, Nováková L, Mladěnka P (2022) Vitamin D: sources, physiological role, biokinetics, deficiency, therapeutic use, toxicity, and overview of analytical methods for detection of vitamin D and its metabolites. Crit Rev Clin Lab Sci 16:1–38. https://doi.org/10.1080/10408363.2022.207059510.1080/10408363.2022.2070595 ↗
3. Gonnet M, Lethuaut L, Boury F (2010) New trends in encapsulation of liposoluble vitamins. J Control Release 146:276–290. https://doi.org/10.1016/j.jconrel.2010.01.03710.1016/j.jconrel.2010.01.037 ↗
4. Nayanathara U, Kermaniyan SS, Such GK (2020) Multicompartment polymeric nanocarriers for biomedical applications. Macromol Rapid Commun 41:e2000298. https://doi.org/10.1002/marc.20200029810.1002/marc.202000298 ↗
5. Thakuria A, Kataria B, Gupta D (2021) Nanoparticle-based methodologies for targeted drug delivery—an insight. J Nanopart Res 23:87. https://doi.org/10.1007/s11051-021-05190-910.1007/s11051-021-05190-9 ↗
6. Hasannia M, Aliabadi A, Abnous K, Taghdisi SM, Ramezani M, Alibolandi M (2022) Synthesis of block copolymers used in polymersome fabrication: application in drug delivery. J Control Release 341:95–117. https://doi.org/10.1016/j.jconrel.2021.11.01010.1016/j.jconrel.2021.11.010 ↗
7. Semo E, Kesselman E, Danino D, Livney YD (2007) Casein micelle as a natural nano-capsular vehicle for nutraceuticals. Food Hydrocolloids 21:936–942. https://doi.org/10.1016/j.foodhyd.2006.09.00610.1016/j.foodhyd.2006.09.006 ↗
8. Almouazen E, Bourgeois S, Jordheim LP, Fessi H, Briancon S (2013) Nano-encapsulation of vitamin D3 active metabolites for application in chemotherapy: formulation study and in vitro evaluation. Pharm Res 30:1137–1146. https://doi.org/10.1007/s11095-012-0949-410.1007/s11095-012-0949-4 ↗
9. Kim HG, Gater DL, Kim YC (2018) Development of transdermal vitamin D3 (VD3) delivery system using combinations of PLGA nanoparticles and microneedles. Drug Deliv Transl Res 8:281–290. https://doi.org/10.1007/s13346-017-0460-x10.1007/s13346-017-0460-x ↗
10. Gupta AA, Kheur S, Badhe RV, Raj AT, Bhonde R, Jaisinghani A, Vyas N, Patil VR, Alhazmi YA, Parveen S, Baeshen HA, Patil S (2021) Assessing the potential use of chitosan scaffolds for the sustained localized delivery of vitamin D. Saudi J Biol Sci 28:2210–2215. https://doi.org/10.1016/j.sjbs.2021.01.00810.1016/j.sjbs.2021.01.008 ↗
11. Tuckey RC, Nguyen MN, Slominski A (2008) Kinetics of vitamin D3 metabolism by cytochrome P450scc (CYP11A1) in phospholipid vesicles and cyclodextrin. Int J Biochem Cell Biol 40:2619–2626. https://doi.org/10.1016/j.biocel.2008.05.00610.1016/j.biocel.2008.05.006 ↗
12. Ghiasi F, Eskandari MH, Golmakani MT, Rubio RG, Ortega F (2021) Build-up of a 3D organogel network within the bilayer shell of nanoliposomes. A novel delivery system for vitamin D3: preparation, characterization, and physicochemical stability. J Agric Food Chem 69:2585–2594. https://doi.org/10.1021/acs.jafc.0c0668010.1021/acs.jafc.0c06680 ↗
13. Yi S, Zhang X, Sangji H, Liu Y, Allen SD, Xiao B, Bobbala S, Braverman CL, Cai L, Hecker PI, DeBerge M, Thorp EB, Temel RE, Stupp SI, Scott EA (2019) Surface engineered polymersomes for enhanced modulation of dendritic cells during cardiovascular immunotherapy. Adv Funct Mater 29:1904399. https://doi.org/10.1002/adfm.20190439910.1002/adfm.201904399 ↗
14. Besada LN, Peruzzo P, Cortizo AM, Cortizo MS (2018) Preparation, characterization, and in vitro activity evaluation of triblock copolymer-based polymersomes for drugs delivery. J Nanopart Res 20:67–79. https://doi.org/10.1007/s11051-018-4169-710.1007/s11051-018-4169-7 ↗
15. Parasassi T, De Stasio G, Ravagnan G, Rusch RM, Gratton E (1991) Quantitation of lipid phases in phospholipid vesicles by the generalized polarization of Laurdan fluorescence. Biophys J 60:179–189. https://doi.org/10.1016/S0006-3495(91)82041-010.1016/S0006-3495(91)82041-0 ↗
16. Henning MF, Sanchez S, Bakás L (2009) Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers. Biochem Biophys Res Commun 383:22–26. https://doi.org/10.1016/j.bbrc.2009.03.11610.1016/j.bbrc.2009.03.116 ↗
17. Lee JCM, Bermudez H, Discher BM, Sheehan MA, Won YY, Bates FS, Discher DE (2001) Preparation, stability, and in vitro performance of vesicles made with diblock copolymers. Biotechnol Bioeng 73:135–145. https://doi.org/10.1002/bit.104510.1002/bit.1045 ↗
18. Raschke WC, Baird S, Ralph P, Nakoinz I (1978) Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15:261–267. https://doi.org/10.1016/0092-8674(78)90101-010.1016/0092-8674(78)90101-0 ↗
19. Torres ML, Fernandez JM, Dellatorre FG, Cortizo AM, Oberti TG (2019) Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering. Algal Res 40:101499. https://doi.org/10.1016/j.algal.2019.10149910.1016/j.algal.2019.101499 ↗
20. Johnston AH, Dalton PD, Newman TA (2010) Polymersomes, smaller than you think: ferrocene as a TEM probe to determine core structure. J Nanopart Res 12:1997–1200. https://doi.org/10.1007/s11051-010-9886-510.1007/s11051-010-9886-5 ↗
21. Habel J, Ogbonna A, Larsen NW, Cherré S, Kynde S, Midtgaard SR, Kinoshita K, Krabbe S, Jensen GV, Hansen JS, Almdal K, Helix-Nielsen C (2015) Selecting analytical tools for characterization of polymersomes in aqueous solution. RSC Adv 5:79924–79946. https://doi.org/10.1039/C5RA16403F10.1039/C5RA16403F ↗
22. Parasassi T, Di Stefano M, Loiero M, Ravagnan G, Gratton E (1994) Influence of cholesterol on phospholipid bilayers phase domains as detected by Laurdan fluorescence. Biophys J 66:120–132. https://doi.org/10.1016/S0006-3495(94)80763-510.1016/S0006-3495(94)80763-5 ↗
23. Bondar OP, Rowe ES (1995) Differential scanning calorimetric study of the effect of vitamin D3 on the thermotropic phase behavior of lipids model systems. Biochim Biophys Acta 1240:125–132. https://doi.org/10.1016/0005-2736(95)00182-410.1016/0005-2736(95)00182-4 ↗
24. Bravi Constantino ML, Cortizo MS, Cortizo AM, Oberti T (2020) Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: designed, properties and biocompatibility studies. Eur Polym J 122:09348. https://doi.org/10.1016/j.eurpolymj.2019.10934810.1016/j.eurpolymj.2019.109348 ↗
25. Handajani J, Tabtila U, Rully Auliawati N, Rohman A (2020) Characterization of buccal cell DNA after exposure to azo compounds: a cross-sectional study. F1000Res 9:1053–1067. https://doi.org/10.12688/f1000research.25798.210.12688/f1000research.25798.2 ↗
26. Puvaneswari N, Muthukrishnan J, Gunasekaran P (2006) Toxicity assessment and microbial degradation of azo dyes. Indian J Exp Biol 44:618–626
27. Sahasrabudhe MM, Saratale RG, Saratale GD, Pathade GR (2014) Decolorization and detoxification of sulfonated toxic diazo dye CI Direct Red 81 by Enterococcus faecalis YZ 66. J Env Health Sci Eng 12:1–13. https://doi.org/10.1186/s40201-014-0151-110.1186/s40201-014-0151-1 ↗
28. Tsuboy MS, Angeli JPF, Mantovani MS, Knasmüller S, Umbuzeiro GA, Ribeiro LR (2007) Genotoxic, mutagenic and cytotoxic effects of the commercial dye CI Disperse Blue 291 in the human hepatic cell line HepG2. Toxicol in vitro 21:1650–1655. https://doi.org/10.1016/j.tiv.2007.06.02010.1016/j.tiv.2007.06.020 ↗
29. Demirbilek M, Laçin Türkoglu N, Aktürk S, Akça C (2017) VitD3-loaded solid lipid nanoparticles: stability, cytotoxicity and cytokine levels. J Microencapsul 34:454–462. https://doi.org/10.1080/02652048.2017.134599510.1080/02652048.2017.1345995 ↗
30. Fernández JM, Cortizo MS, Cortizo AM (2014) Fumarate/ceramic composite based scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility. J Biomat Tissue Eng 4:227–234. https://doi.org/10.1166/jbt.2014.115810.1166/jbt.2014.1158 ↗