In vitro effects of metal oxide nanoparticles on barley oxalate oxidase
Source abstract
Abstract
Barley oxalate oxidase (OxO), a manganese-containing protein, is largely employed for determination of oxalate in various biologic materials. The present report describes in vitro effects of nanoparticles (NPs) of three metal oxides, i.e., zinc oxide (ZnO), copper oxide (CuO), and manganese oxide (MnO2), on the activity and stability of OxO purified from barley roots. The transmission electron microscopy and X-ray diffraction studies of these NPs revealed their very fine crystalline structure with the dimeter in the range 30–70, 50–60, and 20–60 nm for ZnO NPs, CuO NPs, and MnO2 NPs, respectively. The addition of suspension of these three NPs into assay mixture of enzyme individually, led to the adsorption of OxO over their surface, as confirmed by Fourier transform infrared spectra and UV–Vis spectroscopic studies. Compared to free enzyme, MnO2 NPs-bound enzyme showed improved activity (35 % stimulation at 2.5 mg/ml concentration), while ZnO NPs- and CuO NPs-bound enzyme had no substantial improvement. The kinetic properties of individually NPs-bound enzyme were studied and compared with those of free enzyme. The MnO2 NPs-bound enzyme also showed marked improvement in its storage and thermal stability compared to free enzyme.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Kidney Stones and Urolithiasis Treatments · Porphyrin Metabolism and Disorders · Plant pathogens and resistance mechanisms
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (31)
Show source-provided references
1. Aguilar C, Urzúa U, Koenig C, Vicuña R (1999) Oxalate oxidase from Ceriporiopsis subvermispora: biochemical and cytochemical studies. Arch Biochem Biophys 366:275–28210.1006/abbi.1999.1216 ↗
2. Aly HA, Mansour AM, Abo-Salem OM, Abd-Ellah HF, Abdel-Naim AB (2009) Potential testicular toxicity of sodium nitrate in adult rats. Food Chem Toxicol 48:572–57810.1016/j.fct.2009.11.034 ↗
3. Bias R, Potezny N, Edward JB, Rofe AM, Conyer RAJ (1980) Oxalate determination by immobilized oxalate oxidase in a continuous flow system. Anal Chem 52:508–55110.1021/ac50053a030 ↗
4. Cassland P, Sjöde A, Winestrand S, Jönsson LJ, Nilvebrant NO (2010) Evaluation of oxalate decarboxylase and oxalate oxidase for industrial applications. Appl Biochem Biotechnol 61:255–26310.1007/s12010-009-8769-7 ↗
5. Chauhan N, Hooda V, Pundir CS (2011) Influence of copper nanoparticles on copper requiring enzyme. J Exp Nanosci 7:624–63310.1080/17458080.2011.554577 ↗
6. Chauhan N, Narang J, Shweta, Pundir CS (2012) Immobilization of barley oxalate oxidase onto gold-nanoparticle-porous CaCO3 microsphere hybrid for amperometric determination of oxalate in biological materials. Clin Biochem 45:253–25810.1016/j.clinbiochem.2011.12.004 ↗
7. Chena L, Suna LJ, Luana F, Lianga Y, Li Y, Liua XX (2010) Synthesis and pseudocapacitive studies of composite films of polyaniline and manganese oxide nanoparticles. J Power Sources 195:3742–374710.1016/j.jpowsour.2009.12.036 ↗
8. Dahiya T, Pundir CS (2011) Purification and properties of membrane bound oxalate oxidase from leaves of Bougainvillea glabra. Int J Biotech Biochem 7:515–528
9. Dahiya T, Pundir C S (2012) In vivo oxalate degradation by liposome encapsulated oxalate oxidase in rat model of hyperoxaluria. Ind J Med Res (in press)
10. Dahiya T, Yadav S, Chauhan N, Handa P, Pundir CS (2010) Strawberry fruit oxalate oxidase—detection, purification, characterization and physiological role. J Plant Biochem Biotech 19:247–25010.1007/BF03263349 ↗
11. Delisle G, Champoux M, Houde M (2001) Characterization of oxalate oxidase and cell death in Al-sensitive and tolerant wheat roots. Plant Cell Physiol 42:324–33310.1093/pcp/pce041 ↗
12. Godara S, Pundir CS (2008) Urinary and serum oxalate determination by oxalate oxidase immobilized onto affixed arylamine glass beads. Ind J Med Res 127:370–376
13. Graz M, Jarosz-Wilkolazka A, Pawlikoska-Pawlega B (2009) Abortiporus biennis tolerance to insoluble metal oxides: oxalate secretion, oxalate oxidase activity, and mycelia morphology. Biometals 22:401–41010.1007/s10534-008-9176-1 ↗
14. Khobragade CN, Beedkar SD, Bodade RG, Vinchurkar AS (2011) Comparative structural modeling and docking studies of oxalate oxidase: possible implication in enzyme supplementation therapy for urolithiasis. Int J Biol Macromol 48:466–47310.1016/j.ijbiomac.2011.01.007 ↗
15. Kotsira VP, Clonis YD (1997) Oxalate oxidase from barley roots: purification to homogeneity and study of some molecular, catalytic, and binding properties. Arch Biochem Biophys 340:239–24910.1006/abbi.1997.9896 ↗
16. Koyama H (1988) Purification and characterization of oxalate oxidase from Pseudomonas sp. OX-53. Agric Biol Chem 52:743–74810.1271/bbb1961.52.743 ↗
17. Kumar R, Hooda V, Pundir CS (2011) Purification and partial characterization of oxalate oxidase from leaves of forage Sorghum (Sorghum vulgare var. KH-105) seedlings. Ind J Biochem Biophys 48:42–46
18. Lanje AS, Sharma SJ, Pode RB, Ningthoujam RS (2010) Synthesis and optical characterization of copper oxide nanoparticles. Adv Appl Sci Res 1:36–40
19. Li Z, Ding Y, Xiong Y, Yang Q, Xie Y (2005) One-step solution-based catalytic route to fabricate novel α-MnO2 hierarchical structures on a large scale. Chem Commun 7:918–92010.1039/B414204G ↗
20. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–27510.1016/S0021-9258(19)52451-6 ↗
21. Marom H, Antonov S, Popowski Y, Gozin M (2011) Selective sulfoxidation of thioethers and thioaryl boranes with nitrate, promoted by a molybdenum–copper catalytic system. J Org Chem 76:5240–524610.1021/jo2001808 ↗
22. McCarthy MA, Matthews RH (1984) Agriculture handbook No 8–12: composition of foods-nut and seed products. US Government Printing Office, Washington DC
23. Moghaddam AB, Badraghi TJ, Kazemzad M (2009) Synthesis of ZnO nanoparticles and electrodeposition of polypyrrole/ZnO nanocomposite film. Int J Electrochem Sci 4:247–25710.1016/S1452-3981(23)15125-X ↗
24. Opaleye O, Rose RS, Whittaker MM, Woo EJ, Whittaker JW, Pickersgill RW (2006) Structural and spectroscopic studies shed light on the mechanism of oxalate oxidase. J Biol Chem 281:6428–643310.1074/jbc.M510256200 ↗
25. Pundir CS, Kuchhal NK, Bhargava AK (1998) Determination of urinary oxalate with oxalate oxidase and peroxidase immobilized on to glass beads. Biotechnol Appl Biochem 27:103–10710.1111/j.1470-8744.1998.tb01381.x ↗
26. Pundir CS, Chauhan N, Rajneesh, Manjeet, Ravi (2012) A novel amperometric biosensor for oxalate determination using multi-walled carbon nanotube gold nanoparticle composite. Sens Actuators B Chemistry 155:796–80310.1016/j.snb.2011.01.050 ↗
27. Suguira M, Yamamura H, Hiranok, Sasaki M, Morikawa M, Tsuboi M (1979) Purification and properties of oxalate oxidase from barley seedlings. Chem Pharm Bull 27:2003–200710.1248/cpb.27.2003 ↗
28. Telenko PDE, Hu J, Livingstone DM, Shew BB, Phipps PM, Grabau EA (2011) Sclerotinia blight resistance in Virginia-type peanut transformed with a barley oxalate oxidase gene. Phytopathology 101:786–79310.1094/PHYTO-10-10-0266 ↗
29. Tracy CR, Pearle MS (2009) Update on the medical management of stone disease. Curr Opin Urol 19:200–20410.1097/MOU.0b013e328323a81d ↗
30. Wan X, Tan J, Lu S, Lin C, Hu Y, Guo Z (2009) Increased tolerance to oxidative stress in transgenic tobacco expressing a wheat oxalate oxidase gene via induction of antioxidant enzymes is mediated by H2O2. Physiol Plant 136:30–4410.1111/j.1399-3054.2009.01210.x ↗
31. Zhou F, Zhang Z, Gregersen PL, Mikkelsen JD, de Neergaard E, Collinge DB, Thordal-Christensen H (1998) Molecular characterization of the oxalate oxidase involved in the response of barley to the powdery mildew fungus. Plant Physiol 117:33–4110.1104/pp.117.1.33 ↗