Production of anti-fullerene C60 polyclonal antibodies and study of their interaction with a conjugated form of fullerene
Source abstract
Abstract
The aim of this study was to produce anti- fullerene C60 antibodies for the development of detection systems for fullerene C60 derivatives. To produce anti-fullerene C60 antibodies, conjugates of the fullerene C60 carboxylic derivative with thyro- globulin, soybean trypsin inhibitor, and bovine serum albumin were synthesized by carbodiimide activation and characterized. Immunization of rabbits by the conjugates led to the production of polyclonal anti- fullerene antibodies. The specificity of the immune response to fullerene was investigated. Indirect competitive immunoenzyme assay was developed for the determination of conjugated fullerene with detection limits of 0.04 ng/mL (calculated for cou- pled C60) and 0.4 ng/mL (accordingly to total fuller- ene-protein concentration).
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Fullerene Chemistry and Applications · Carbon Nanotubes in Composites · Graphene and Nanomaterials Applications
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