Aptamer- and antibody-conjugated gold nanobipyramids—a study on cytotoxicity towards breast cancer cell lines
Source abstract
Abstract
Specifically targeted gold nanobipyramids (GNBs) hold great potential as photothermal therapeutic agent apart from various other applications such as contrast agents in diagnostic imaging techniques, biosensors, drug delivery, and hence their cytotoxicity need to be determined. Further, the efficiency of specifically targeted therapeutic delivery depends on various factors such as expression levels of target protein, targeting moiety, route of administration, and location of tissue. Hence, it is desirable to understand the efficiency of targeting moieties such as aptamers and antibodies in specifically targeting their target protein and their effect on cytotoxicity. Studies comparing the targeting efficiency of aptamer- and antibody-conjugated gold nanobipyramids on their cytotoxicity; targeting ability and photothermal effect are very scarce. In this perspective, present study investigates the photothermal properties of gold nanobipyramids and compares the effect of targeting moiety against MUC1 protein on cell cytotoxicity and thermal effect on MCF7 cell lines and HCT116 cell lines. Transmission electron microscopy (TEM) micrographs represented that the synthesized gold nanostructures were of bipyramidal shape with an average length of 71.73 nm and width of 27.7 nm. The results showed that conjugation with aptamer had greatly reduced the cytotoxicity of nanobipyramids on both the cell lines compared to antibody conjugation. In vitro photoablation studies revealed that aptamer-conjugated nanobipyramids were more efficient in specifically photoablating MCF7 cell lines w.r.t HCT116 cell lines in comparison to antibody-conjugated nanobipyramids which were consistent with cellular uptake studies. Graphical Abstract
Source: expertdata · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Advanced biosensing and bioanalysis techniques · Gold and Silver Nanoparticles Synthesis and Applications · RNA Interference and Gene Delivery
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
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