Human T-cell line (Jurkat cell) encapsulation by nano-organized polyelectrolytes and their response assessment in vitro and in vivo
Source abstract
Abstract
Single living Jurkat cells have been encapsulated through polyelectrolytes nanoparticles poly(allyl amine hydrochloride) size 15.6 nm and poly(styrene-co-sulfonic acid sodium salt) size 30.2 nm, through layer by layer coating of oppositely charged plasma membrane. Confocal microscopy and scanning electron microscopy results showed complete shielding of Jurkat cells, and no changes in cell surface morphology of encapsulated cells were observed. Cell viability was not affected after encapsulation and no toxicity was found. In vivo studies demonstrated no significant changes in hematological and biochemical parameters of blood serum at day 1 and 7 in mice. Histopathological analyses of liver and spleen tissues showed nontoxic nature of prepared formulations.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Advanced Drug Delivery Systems · Nanoparticle-Based Drug Delivery · Polymer Surface Interaction Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (15)
Show source-provided references
1. Ai H, Jones SA, Lvov YM (2003) Biomedical applications of electrostatic layer-by-layer nano-assembly of polymers, enzymes and nanoparticles. Cell Biochem Biophys 39(1):23–4310.1385/CBB:39:1:23 ↗
2. Collis SJ, Neutzel S, Thompson TL, Swartz MJ, Dillehay LE, Collector MI, Sharkis SJ, DeWeese TL (2004) Hematopoietic progenitor stem cell homing in mice lethally irradiated with ionizing radiation at differing dose rates. Radiat Res 162(1):48–5510.1667/RR3197 ↗
3. Diaspro A, Silvano D, Krol S, Cavalleri O, Gliozzi A (2002) Single living cell encapsulation in nano-organized polyelectrolyte shells. Langmuir 18(13):5047–505010.1021/la025646e ↗
4. Granicka LH, Antosiak-Iwanska M, Godlewska E, Hoser G, Strawski M, Szklarczyk M, Dudzinski K (2009) The experimental study of polyelectrolyte coatings suitability for encapsulation of cells. Artif Cells Blood Substit Immobil Biotechnol 37(5):187–19410.1080/10731190903199218 ↗
5. Hillberg AL, Tabrizian M (2006) Biorecognition through layer-by-layer polyelectrolyte assembly: in-situ hybridization on living cells. Biomacromolecules 7(10):2742–275010.1021/bm060266j ↗
6. Hua A, Ming F, Jones SA, Lvov YM (2002) Electrostatic Layer-by-Layer Nanoassembly on Biological Microtemplates: platelets. Biomacromolecules 3(3):560–56410.1021/bm015659r ↗
7. Jan E, Kotov NA (2007) Successful differentiation of mouse neural stem cells on layer-by-layer assembled single-walled carbon nanotube composite. Nano Lett 7(5):1123–112810.1021/nl0620132 ↗
8. Kerdjoudj H, Berthelemy N, Rinckenbach S, Kearney-Schwartz A, Montagne K, Schaaf P, Lacolley P, Stoltz JF, Voegel JC, Menu P (2008) Small vessel replacement by human umbilical arteries with polyelectrolyte film-treated arteries: in vivo behavior. J Am Coll Cardiol 52(19):1589–159710.1016/j.jacc.2008.08.009 ↗
9. Lvov Y, Antipov AA, Mamedov A, Mohwald H, Sukhorukov GB (2001) Urease encapsulation in nanoorganized microshells. Nano Lett 1(3):125–12810.1021/nl0100015 ↗
10. Mansouri S, Merhi Y, Winnik FM, Tabrizian M (2011) Investigation of layer by layer assembly of polyelectrolyte on fully functional human red blood cells in suspension for attenuated immune response. Biomacromolecules 12(3):585–59210.1021/bm101200c ↗
11. Semenov OV, Malek A, Bittermann AG, Voros J, Zisch AH (2009) Engineered polyelectrolyte multilayer substrates for adhesion, proliferation, and differentiation of human mesenchymal stem cells. Tissue Eng Part A 15(10):2977–299010.1089/ten.tea.2008.0602 ↗
12. Sukhorukov GB, Donath E, Lichtenfeld H, Knippel E, Knippel M, Budde A, Mohwald H (1998) Layer-by-layer self-assembly of polyelectrolytes on colloidal particles. Colloids Surf A 137:253–26610.1016/S0927-7757(98)00213-1 ↗
13. Veerabadran NG, Goli PL, Stewart-Clark SS, Lvov YM, Mills DK (2007) Nanoencpasulation of stem cells within polyelectrolyte multilayer shells. Macromol Biosci 9(7):877–88210.1002/mabi.200700061 ↗
14. Wilson JT, Cui W, Kozlovskaya V, Kharlampieva E, Pan D, Qu Z, Krishnamurthy VR, Mets J, Kumar V, Wen J, Song Y, Tsukruk VV, Chaikof EL (2011) Cell surface engineering with polyelectrolyte multilayer thin films. J Am Chem Soc 113(18):7054–706410.1021/ja110926s ↗
15. Xie S, Wang F, Wang Y, Zhu L, Dong Z, Wang X, Li X, Zhou WZ (2011) Acute toxicity study of tilmicosin-loaded hydrogenated castor oil-solid lipid nanoparticles. Part Fibre Toxicol 8(33):1–10