Spectrofluorimetric study on in vitro interaction between calcium phosphate nanoparticle and salmon testis DNA
Source abstract
Abstract
At present, calcium phosphate nanoparticle (CPNP) is considered as a potential vector of nucleic acids into cells. However, the mode of interaction between CPNP and DNA is quite unknown. This communication throws some light on this. For the investigation, we synthesized CPNP using calcium nitrate and di-ammonium hydrogen phosphate as precursors and Na-citrate as stabilizer. Characterization of the NPs by the techniques of scanning and transmission electron microscopies, electron diffraction X-ray spectroscopy, and X-ray diffraction spectroscopy signified that the particles were about 45 nm in size, spherical in shape, and composed of the hydroxyapatite form of calcium phosphate. The most interesting feature of CPNP was that it had fluorescence with excitation and emission at 254 and 310 nm, respectively. Analysis of the results of different fluorescence-based experiments revealed that the CPNP had specifically bound at the grooves of DNA forming a ground state complex with 1:1 stoichiometry, and the interaction was exothermic, spontaneous, and enthalpy-driven in nature.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Nanoparticle-Based Drug Delivery · Polymer Surface Interaction Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (37)
Show source-provided references
1. Alexander LE, Klug HP (1954) X-ray diffraction procedures for polycrystalline and amorphous material. Wiley, New York
2. Bailly C, Chessari G, Carrasco C, Joubert A, Mann J, Wilson WD, Neidle S (2003) Sequence-specific minor groove binding by bis-benzimidazoles: water molecules in ligand recognition. Nucl Acids Res 31:1514–152410.1093/nar/gkg237 ↗
3. Baily C, Pommery N, Houssin R, Henichart JP (1989) Design, synthesis, DNA binding, and biological activity of a series of DNA minor-groove-binding intercalating drugs. J Pharm Sci 78:910–91710.1002/jps.2600781106 ↗
4. Barrère F, van Blitterswijk CA, de Groot K (2006) Bone regeneration: molecular and cellular interactions with calcium phosphate ceramics. Int J Nanomed 1:317–332
5. Bose S, Saha KS (2003) Synthesis and characterization of hydroxyapatite nanopowders by emulsion technique. Chem Mater 15:4464–446910.1021/cm0303437 ↗
6. Chaires JB (2008) Calorimetry and thermodynamics in drug design. Annu Rev Biophys 37:135–15110.1146/annurev.biophys.36.040306.132812 ↗
7. Dorozhkin SV (2007) Calcium orthophosphates. J Mater Sci 42:1061–109510.1007/s10853-006-1467-8 ↗
8. Erdem A, Ozsoz M (2002) Electrochemical DNA biosensors based on DNA–drug interactions. Electroanal 14:965–97410.1002/1521-4109(200208)14:14<965::AID-ELAN965>3.0.CO;2-U ↗
9. Fei Y, Lu G, Fan G, Wu Y (2009) Spectroscopic studies on the binding of a new quinolone antibacterial agent: sinafloxacin to DNA. Anal Sci 25:1333–133810.2116/analsci.25.1333 ↗
10. Giger EV, Puigmartí-Luis J, Schlatter R, Castagner B, Dittrich PS, Leroux C (2011) Gene delivery with bisphosphonate-stabilized calcium phosphate nanoparticles. J Control Release 150:87–9310.1016/j.jconrel.2010.11.012 ↗
11. Graham FL, van der Eb AJ (1973) A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–46710.1016/0042-6822(73)90341-3 ↗
12. He Q, Mitchell AR, Johnson SL, Wagner-Bartak C, Morcol T, Bell SJD (2000) Calcium phosphate nanoparticle adjuvant. Clin Vaccine Immunol 7:899–90310.1128/CDLI.7.6.899-903.2000 ↗
13. Jiang M, Xie MX, Zheng D, Liu Y, Li XY, Chen X (2004) Spectroscopic studies on the interaction of cinnamic acid and its hydroxyl derivatives with human serum albumin. J Mol Struct 692:71–8010.1016/j.molstruc.2004.01.003 ↗
14. Kathiravan A, Renganathan R (2009) Photoinduced interactions between colloidal TiO2 nanoparticles and calf thymus-DNA. Polyhedron 28:1374–137810.1016/j.poly.2009.02.040 ↗
15. Kumar CV, Asuncion EH (1993) DNA binding studies and site selective fluorescence sensitization of an anthryl probe. J Am Chem Soc 115:8547–855310.1021/ja00072a004 ↗
16. Lak A, Mazloumi M, Mohajerani MS, Zanganeh S, Shayegh MR, Kajbafvala A, Arami H, Sadrnezhaadw SK (2008) Rapid formation of mono-dispersed hydroxyapatite nanorods with narrow-size distribution via microwave irradiation. J Am Ceram Soc 91:3580–358410.1111/j.1551-2916.2008.02690.x ↗
17. Lakowicz JR (1973) Principle of fluorescence spectroscopy, 1st edn. Plenium Press, New York, pp 258–301
18. Lechardeur D, Verkman AS, Lukacs GL (2005) Intracellular routing of plasmid DNA during non viral gene transfer. Adv Drug Delivery Rev 57:755–76710.1016/j.addr.2004.12.008 ↗
19. Ling X, Zhong W, Huang Q, Ni K (2008) Spectroscopic studies on the interaction of pazufloxacin with calf thymus DNA. J Photochem Photobiol B 93:172–17610.1016/j.jphotobiol.2008.07.008 ↗
20. Liu R, Yang J, Wu X (2002) Study of the interaction between nucleic acid and oxytetracycline–Eu3þ and its analytical application. J Lumin 96:201–20910.1016/S0022-2313(01)00234-4 ↗
21. Liu W, Sun S, Cao Z, Zhanga X, Yao K, Lub WW, Luk KDK (2005) An investigation on the physicochemical properties of chitosan/DNA polyelectrolyte complexes. Biomaterials 26:2705–271110.1016/j.biomaterials.2004.07.038 ↗
22. Liu YC, Wang T, He FL, Liu Q, Zhang DX, Xiang SL, Su SP, Zhang J (2011) An efficient calcium phosphate nanoparticle-based nonviral vector for gene delivery. Int J Nanomed 6:721–72710.2147/IJN.S17096 ↗
23. Mazur S, Tanious FA, Ding D, Kumar A, Boykin DW, Simpson JI, Neidle S, Wilson WD (2000) A thermodynamic and structural analysis of DNA minor-groove complex formation. J Mol Biol 300:321–33710.1006/jmbi.2000.3869 ↗
24. Milev A, Kannangara GSK, Ben-Nissan B (2003) Morphological stability of hydroxyapatite precursor. Mater Lett 57:1960–196510.1016/S0167-577X(02)01112-6 ↗
25. Mondéjar SP, Kovtun A, Epple M (2007) Lanthanide-doped calcium phosphate nanoparticles with high internal crystallinity and with a shell of DNA as fluorescent probes in cell experiments. J Mater Chem 17:4153–415910.1039/b708258d ↗
26. Oltona D, Li J, Wilsona ME, Rogers T, Closed J, Huange L, Kumta PN, Sfeir C (2007) Nanostructured calcium phosphates (NanoCaPs) for non-viral gene delivery: influence of the synthesis parameters on transfection efficiency. Biomater J 28:1267–127910.1016/j.biomaterials.2006.10.026 ↗
27. Parodi S, Kendall F, Nicolini C (1975) A clarification of the complex spectrum observed with the ultraviolet circular dichroism of ethidium bromide bound to DNA. Nucl Acid Res 2:477–48610.1093/nar/2.4.477 ↗
28. Prabakaran N, Athappan P (2010) DNA assisted fragmentation of nickel nanoparticle clusters and their spectral properties. J Inorg Biochem 104(7):712–71710.1016/j.jinorgbio.2010.03.003 ↗
29. Rajdev P, Mondol T, Makhal A, Pal SK (2011) Simultaneous binding of anti-tuberculosis and anti-thrombosis drugs to a human transporter protein: a FRET study. J Photochem Photobiol B 103:153–15810.1016/j.jphotobiol.2011.02.023 ↗
30. Roy I, Mitra S, Maitra A, Mozumdar S (2003) Calcium phosphate nanoparticles as novel non-viral vectors for targeted gene delivery. Int J Pharm 250:25–3310.1016/S0378-5173(02)00452-0 ↗
31. Scaria PV, Shafer RH (1991) Binding of \to a DNA triple helix. J Biol Chem 266:5417–542310.1016/S0021-9258(19)67611-8 ↗
32. Schütz E, von Ahsen N (2009) Influencing factors of dsDNA dye (high-resolution) melting curves and improved genotype call based on thermodynamic considerations. Anal Biochem 385:143–15210.1016/j.ab.2008.10.045 ↗
33. Sokolova V, Radtkeb HR, Epple M (2006) Effective transfection of cells with multi-shell calcium phosphate-DNA nanoparticles. Biomater J 27:3147–315310.1016/j.biomaterials.2005.12.030 ↗
34. Sokolova V, Kovtun A, Heumann R, Epple M (2007a) Tracking the pathway of calcium phosphate/DNA nanoparticles during cell transfection by incorporation of red-fluorescing tetramethylrhodamine isothiocyanate–bovine serum albumin into these nanoparticles. J Biol Inorg Chem 12:174–17910.1007/s00775-006-0177-7 ↗
35. Sokolova V, Kovtun A, Prymak O, Meyer-Zaika W, Kubareva EA, Romanova EA, Oretskaya TS, Heumannc R, Epple M (2007b) Functionalisation of calcium phosphate nanoparticles by oligonucleotides and their application for gene silencing. J Mater Chem 17:721–72710.1039/B612699E ↗
36. Zhang K, Fang H, Shen G, Taylor J-SA, Wooley KL (2009) Well-defined cationic shell crosslinked nanoparticles (SCKs) for efficient delivery of DNA or PNA. Proc Am Thorac Soc 6:450–45710.1513/pats.200902-010AW ↗
37. Ye BF, Ju HX (2003) Determination of yeast DNA based on its quenching the fluorescence emission of norfloxacin. Anal Lett 36:1351–136410.1081/AL-120021091 ↗