Fullerene (C60) nanoparticles exert photocytotoxicity through modulation of reactive oxygen species and p38 mitogen-activated protein kinase activation in the MCF-7 cancer cell line
Source abstract
Abstract
The photocytotoxicity of water-dispersed 100–300 nm fullerene amino acid derivatives nanoparticles was studied. The nanoparticle solution of fullerene derivatives, l-phenylalanine (C60-phe) and glycine (C60-gly), suppressed the in vitro growth of MCF-7 cells lines, induced cancer cells apoptosis, and caused a perturbation of the cell cycle. These nanoparticle solutions increased intracellular reactive oxygen species after irradiation. C60-phe or C60-gly upregulated the expression of phosphorylated (p)p38 mitogen-activated protein kinase (MAPK). N-Acetyl-l-cysteine significantly depressed the composite-induced activation of p38MAPK, and the kinase inhibitor SB203580 significantly prevented C60 derivative-induced cell apoptosis. This study revealed that p38MAPK is activated by C60 nanoparticles through triggering reactive oxygen species generation, leading to cancer cell injuries.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Fullerene Chemistry and Applications · Carbon Nanotubes in Composites · Nanoparticles: synthesis and applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (25)
Show source-provided references
1. Bolskar RD, Benedetto AF, Husebo LO, Price RE, Jackson EF, Wallace S, Wilson LJ, Alford JM (2003) First soluble M@C-60 derivatives provide enhanced access to metallofullerenes and permit in vivo evaluation of Gd@C-60[C(COOH)(2)](10) as a MRI contrast agent. J Am Chem Soc 125(18):5471–547810.1021/ja0340984 ↗
2. Bosi S, Da Ros T, Spalluto G, Prato M (2003) Fullerene derivatives: an attractive tool for biological applications. Eur J Med Chem 38(11–12):913–92310.1016/j.ejmech.2003.09.005 ↗
3. Cui WJ, Liu Y, Zhou XL, Wang FZ, Zhang XD, Ye LH (2010) Myosin light chain kinase is responsible for high proliferative ability of breast cancer cells via antiapoptosis involving p38 pathway. Acta Pharmacol Sin 31(6):725–73210.1038/aps.2010.56 ↗
4. Ding X, Xu Q, Liu F, Zhou P, Gu Y, Zeng J, An J, Dai W, Li X (2004) Hematoporphyrin monomethyl ether photodynamic damage on HeLa cells by means of reactive oxygen species production and cytosolic free calcium concentration elevation. Cancer Lett 216(1):43–5410.1016/j.canlet.2004.07.005 ↗
5. Gan L, Zhou D, Luo C, Tan H, Huang C, Lü M, Pan J, Wu Y (1996) Synthesis of fullerene amino acid derivations by direct interaction of amino acid ester with C60. J Org Chem 61(6):1954–196110.1021/jo951933u ↗
6. Ji RR, Woolf CJ (2001) Neuronal plasticity and signal transduction in nociceptive neurons: implications for the initiation and maintenance of pathological pain. Neurobiol Dis 8(1):1–1010.1006/nbdi.2000.0360 ↗
7. Kroto HW, Heath JR (1985) C60: Buckminsterfullerene. Nature 318(6042):162–16310.1038/318162a0 ↗
8. Lan AP, Xiao LC, Yang ZL, Yang CT, Wang XY, Chen PX, Gu MF, Feng JQ (2012) Interaction between ROS and p38MAPK contributes to chemical hypoxia-induced injuries in PC12 cells. Mol Med Rep 5(1):250–255
9. Liu J, Tabata Y (2010) Photodynamic therapy of fullerene modified with pullulan on hepatoma cells. J Drug Target 18(8):602–61010.3109/10611861003599479 ↗
10. Marchesan S, Da Ros T, Spalluto G, Balzarini J, Prato M (2005) Anti-HIV properties of cationic fullerene derivatives. Bioorg Med Chem Lett 15(15):3615–361810.1016/j.bmcl.2005.05.069 ↗
11. Markovic Z, Trajkovic V (2008) Biomedical potential of the reactive oxygen species generation and quenching by fullerenes (C60). Biomaterials 29(26):3561–357310.1016/j.biomaterials.2008.05.005 ↗
12. Moserova I, Kralova J (2012) Role of ER stress response in photodynamic therapy: ROS generated in different subcellular compartments trigger diverse cell death pathways. PLoS ONE 7(3):e3297210.1371/journal.pone.0032972 ↗
13. Mroz P, Pawlak A, Satti M, Lee H, Wharton T, Gali H, Sarna T, Hamblin MR (2007) Functionalized fullerenes mediate photodynamic killing of cancer cells: Type I versus Type II photochemical mechanism. Free Radic Biol Med 43(5):711–71910.1016/j.freeradbiomed.2007.05.005 ↗
14. Nusuetrong P, Saito M, Kikuchi H, Oshima Y, Moriya T, Nakahata N (2012) Apoptotic effects of satratoxin H is mediated through DNA double-stranded break in PC12 cells. J Toxicol Sci 37(4):803–81210.2131/jts.37.803 ↗
15. Olive PL, Banath JP (2006) The comet assay: a method to measure DNA damage in individual cells. Nat Protoc 1(1):23–2910.1038/nprot.2006.5 ↗
16. Otake E, Sakuma S, Torii K, Maeda A, Ohi H, Yano S, Morita A (2010) Effect and mechanism of a new photodynamic therapy with glycoconjugated fullerene. Photochem Photobiol 86(6):1356–136310.1111/j.1751-1097.2010.00790.x ↗
17. Park SY, Kim YH, Kim YH, Lee SJ (2010) 1,3-Dichloro-2-propanol induces apoptosis via both calcium and ROS in mouse melanoma cells. Biotechnol Lett 32(1):45–5110.1007/s10529-009-0117-z ↗
18. Partha R, Lackey M, Hirsch A, Casscells SW, Conyers JL (2007) Self assembly of amphiphilic C60 fullerene derivatives into nanoscale supramolecular structures. J Nanobiotechnol 5(6):1–11
19. Prylutska SV, Grynyuk II, Palyvoda KO, Matyshevska OP (2010) Photoinduced cytotoxic effect of fullerenes C60 on transformed T-lymphocytes. Exp Oncol 32(1):29–32
20. Schinazi RF, Sijbesma R, Srdanov G, Hill CL, Wudl F (1993) Synthesis andvirucidal activity of a water-soluble, configurationally stable, derivatized C60 fullerene. Antimicrob Agents Chemother 37(8):1707–171010.1128/AAC.37.8.1707 ↗
21. Schutte B, Nuydens R, Geerts H, Ramaekers F (1998) Annexin V binding assay as a tool to measure apoptosis in differentiated neuronal cells. J Neurosci Methods 86(1):63–6910.1016/S0165-0270(98)00147-2 ↗
22. Su HL, Chou CC, Hung DJ, Lin SH, Pao IC, Lin JH, Huang FL, Dong RX, Lin JJ (2009) The disruption of bacterial membrane integrity through ROS generation induced by nanohybrids of silver and clay. Biomaterials 30(30):598–5979
23. Widmann C, Gibson S, Jarpe MB, Johnson GL (1999) Mitogen-activated protein kinase: conservation of a three-kinase module from yeast to human. Physiol Rev 79:143–18010.1152/physrev.1999.79.1.143 ↗
24. Yin JJ, Lao F, Meng J, Fu PP, Zhao Y, Xing G, Gao X, Sun B, Wang PC, Chen C, Liang XJ (2008) Inhibition of tumor growth by endohedral metallofullerenol nanoparticles optimized as reactive oxygen species scavenger. Mol Pharmacol 74(4):1132–114010.1124/mol.108.048348 ↗
25. Zhu Z, Schuster DI, Tuckerman ME (2003) Molecular dynamics study of the connection between flap closing and binding of fullerene-based inhibitors of the HIV-1 protease. Biochemistry 42(5):1326–133310.1021/bi020496s ↗