Transportation of drug–gold nanocomposites by actinomyosin motor system
Source abstract
Abstract
Nanotechnology is playing an important role in drug delivery to overcome limitations of conventional drug delivery systems in terms of solubility, in vivo stability, pharmacokinetics, and bio-distribution. The controlled transportation of drug into the cell and within the cell is a major challenge to be addressed. Cellular molecular motors have been exploited for their cargo carrying capacity for various applications including engineering and health care. Combination of nanotechnology and biomolecular motors can address some of the challenges in drug delivery. In the present study, transportation of drug nanocomposites has been demonstrated. Nanocomposites of 6-mercaptopurine and levodopa drugs (cancer and Parkinson’s disease, respectively) were prepared with gold nanoparticles (GNPs) by covalent attachment and these nanocomposites were attached to actin filaments. These nanocomposites were in-turn transported by actin filaments on myosin tracks. Characterization of drug nanocomposites formation was done by UV–Vis spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and confocal microscopy. GNP composites of 6-mercaptopurine and levodopa were formed by sulfide and amide bond formation, respectively. Average velocity of actin filament attached to nanocomposites was found to be 3.17 and 3.89 μm/s for levodopa and 6-mercaptopurine, respectively, as compared to actin filaments with velocity of 4.0–6.0 μm/s. Three concepts have been proposed for the study of drug transportation into the cell based on polycationic complex formation, interaction of actin with cellular myosin and Biomolecular Adaptor for Retrograde Transport (BART) technology. The aspects of this study heads toward the development of an approach to utilize molecular motors for nanoscale transportation endogenously.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanoparticle-Based Drug Delivery · Polymer Surface Interaction Studies · Micro and Nano Robotics
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (31)
Show source-provided references
1. Agasti SS, Chompoosor A, You CC, Ghosh P, Kim CK, Rotello VM (2009) Photoregulated release of caged anticancer drugs from gold nanoparticles. J Am Chem Soc 131:5728–572910.1021/ja900591t ↗
2. Alisar SZ, de Melgardt V, Michael VP (2005) Encapsulation of drug nanoparticles in self-assembled macromolecular nanoshells. Langmuir 21:403–41010.1021/la0478595 ↗
3. Cohen RN, Rashkin MJ, Wen X, Szoka FC Jr (2005) Molecular motors as drug delivery vehicles. Drug Discov Today Technol 2:111–11810.1016/j.ddtec.2005.04.003 ↗
4. Couvreur P, Vauthier C (2006) Nanotechnology: intelligent design to treat complex disease. Pharm Res 23:1417–145010.1007/s11095-006-0284-8 ↗
5. Daniel ME, Astruc D (2004) Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev 104:293–34610.1021/cr030698+ ↗
6. Diez S, Reuther C, Dinu C, Seidel R, Mertig M, Pompe W, Howard J (2003) Stretching and transporting DNA molecules using motor proteins. Nano Lett 3:1251–125410.1021/nl034504h ↗
7. Emerich DF, Thanos CG (2006) The pin point promises of nanoparticles-based drug delivery and molecular diagnosis. Biomol Eng 23:171–18410.1016/j.bioeng.2006.05.026 ↗
8. Goldstein LSB (2001) Molecular motors—from one motor many tails to one motor many tales. Trends Cell Biol 11:477–48210.1016/S0962-8924(01)02143-2 ↗
9. Han G, Ghosh P, De M, Rotello VM (2007) Drug and gene delivery using gold nanoparticles. Nanobiotechnology 3:40–4510.1007/s12030-007-0005-3 ↗
10. Hosseinkhani H, Hosseinkhani M (2009) Biodegradable polymer–metal complexes for gene and drug delivery. Curr Drug Saf 6:79–8310.2174/157488609787354477 ↗
11. Hosseinkhani H, Tabata Y (2003) In vitro gene expression by cationized derivatives of an artificial protein with repeated RGD sequences, Pronectin. J Control Release 86:169–18210.1016/S0168-3659(02)00412-1 ↗
12. Hosseinkhani H, Tabata Y (2005) Ultrasound enhances in vivo tumor expression of plasmid DNA by PEG-introduced cationized dextran. J Control Release 108:540–55610.1016/j.jconrel.2005.08.027 ↗
13. Hosseinkhani H, Tabata Y (2006) Self assembly of DNA nanoparticles with polycations for the delivery of genetic materials into cells. J Nanosci Nanotechnol 6:2320–232810.1166/jnn.2006.507 ↗
14. Hosseinkhani H, Azzam T, Tabata Y, Domb AJ (2004) Dextran–spermine polycation: an efficient nonviral vector for in vitro and in vivo gene transfection. Gene Ther 11:194–20310.1038/sj.gt.3302159 ↗
15. Hosseinkhani H, Hosseinkhani M, Gabrielson NP, Pack DW, Khademhosseini A, Kobayashi H (2008) DNA nanoparticles encapsulated in 3D tissue-engineered scaffolds enhances osteogenic differentiation of mesenchymal stem cells. J Biomed Mater Res A 85:47–6010.1002/jbm.a.31327 ↗
16. Kamal A, Goldsmith LSB (2002) Principal of cargo attachment to cytoplasmic motor proteins. Curr Opin Cell Biol 14:63–6810.1016/S0955-0674(01)00295-2 ↗
17. Karcher RL (2002) Motor-cargo interactions: the key to transport specificity. Trends Cell Biol 12:21–2710.1016/S0962-8924(01)02184-5 ↗
18. Kaur H, Das T, Kumar R, Ajore R, Bharadwaj LM (2008) Covalent attachment of actin filaments to tween 80 coated polystyrene beads for cargo transportation. Biosystems 92:69–7510.1016/j.biosystems.2007.12.003 ↗
19. Kawaguchi T, Hajime H (2006) Unidirectional movement of an actin filament taking advantage of temperature gradients. Biosystems 90:252–263
20. Kimling J, Maier M, Okenve B, Kotaidis V, Ballot H, Plech A (2006) Turkevich method for gold nanoparticle synthesis revisited. J Phys Chem B 110:15700–1570710.1021/jp061667w ↗
21. Konishia M, Tabatab Y, Kariyaa M, Hosseinkhani H, Suzukia A, Fukuharaa K, Mandaia M, Takakuraa K, Fujiia S (2005) In vivo anti-tumor effect of dual release of cisplatin and adriamycin from biodegradable gelatin hydrogel. J Control Release 103:7–1910.1016/j.jconrel.2004.11.014 ↗
22. Mallik R, Gross SP (2004) Molecular motors: strategies to get along. Curr Biol 14:R971–R98210.1016/j.cub.2004.10.046 ↗
23. Mansson A, Sundberg M, Balaz M, Bunk R, Nicholls IA, Omling P, Tagerud S, Montelius L (2004) In vitro sliding of actin filaments labelled with single quantum dots. Biochem Biophys Res Commun 314:529–53410.1016/j.bbrc.2003.12.133 ↗
24. Mansson A, Sundberg M, Bunk R, Balaz M, Nicholls IA, Omling P, Tegenfeldt JO, Tagerud S, Montelius L (2005) Actin-based molecular motors for cargo transportation in nanotechnology—potentials and challenges. IEEE Trans Adv Packag 28:547–55410.1109/TADVP.2005.858309 ↗
25. Miyata H, Hakozaki H, Yoshikawa H, Suzuki N, Kinosita K Jr, Nishizaka T, Ishiwata S (1994) Stepwise motion of an actin filament over a small number of heavy meromyosin molecules is revealed in an in vitro motility assay. J Biochem 115:644–64710.1093/oxfordjournals.jbchem.a124389 ↗
26. Mohan J (2001) Handbook of organic spectroscopy: principles and applications. Narosa, London
27. Paciotti GF, Myer L, Weinreich D, Goia D, Pavel N, McLaughlin RE, Tamarkin L (2004) Colloidal gold: a novel nanoparticle vector for tumor directed drug delivery. Drug Deliv 11:169–18310.1080/10717540490433895 ↗
28. Pearl C, Reisler E (1982) The actomyosin ATPase of synthetic myosin minifilaments, filaments, and heavy meromyosin. J Biol Chem 258:5040–5044
29. Subramani K, Hosseinkhani H, Khraisat A, Hosseinkhani M, Pathak Y (2009) Targeting nanoparticles as drug delivery systems for cancer treatment. Curr Nanosci 5:134–14010.2174/157341309788185406 ↗
30. Tang JX, Janmey PA (1996) The polyelectrolyte nature of F-actin and the mechanism of actin bundle formation. J Biol Chem 271:8556–856310.1074/jbc.271.15.8556 ↗
31. Vale RD (2003) The molecular motor toolbox for intracellular transport. Cell 112:467–48010.1016/S0092-8674(03)00111-9 ↗