A Promising Combo Gene Delivery System Developed from (3-Aminopropyl)triethoxysilane-Modified Iron Oxide Nanoparticles and Cationic Polymers
Source abstract
Abstract
(3-Aminopropyl)triethoxysilane-modified iron oxide nanoparticles (APTES-IONPs) have been evaluated for various biomedical applications, including medical imaging and drug delivery. Cationic polymers (CPs) such as Lipofectamine and TurboFect are widely used for research in gene delivery, but their toxicity and low in vivo efficiency limited their further application. In the present study, we synthesized water-soluble APTES-IONPs and developed a combo gene delivery system based on APTES-IONPs and CPs. This system significantly increased gene-binding capacity, protected genes from degradation, and improved gene transfection efficiency for DNA and siRNA in both adherent and suspension cells. Because of its great biocompatibility, high gene-carrying ability, and very low cytotoxicity, this combo gene delivery system will be expected for a wide application, and it might provide a new method for gene therapy.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: RNA Interference and Gene Delivery · Advanced biosensing and bioanalysis techniques · Graphene and Nanomaterials Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (25)
Show source-provided references
1. Arsianti M, Lim M, Marquis CP, Amal R (2010) Assembly of polyethylenimine-based magnetic iron oxide vectors: insights into gene delivery. Langmuir 26(10):7314–732610.1021/la9041919 ↗
2. Cheang TY, Tang B, Xu AW, Chang GQ, Hu ZJ, He WL, Xing ZH, Xu JB, Wang M, Wang SM (2011) Promising plasmid DNA vector based on APTES-modified silica nanoparticles. Int J Nanomedicine 7:1061–1067
3. Fang C, Veiseh O, Kievit F, Bhattarai N, Wang F, Stephen Z, Li C, Lee D, Ellenbogen RG, Zhang M (2010) Functionalization of iron oxide magnetic nanoparticles with targeting ligands: their physicochemical properties and in vivo behavior. Nanomedicine 5(9):1357–136910.2217/nnm.10.55 ↗
4. Gan S, Yang P, Yang W (2009) Photoactivation of alkyl C–H and silanization: a simple and general route to prepare high-density primary amines on inert polymer surfaces for protein immobilization. Biomacromolecules 10(5):1238–124310.1021/bm900011h ↗
5. Ghosh R, Singh LC, Shohet JM, Gunaratne PH (2013) A gold nanoparticle platform for the delivery of functional microRNAs into cancer cells. Biomaterials 34(3):807–81610.1016/j.biomaterials.2012.10.023 ↗
6. Jahn MR, Shukoor I, Tremel W, Wolfrum U, Kolb U, Nawroth T, Langguth P (2011) Hemin-coupled iron(III)-hydroxide nanoparticles show increased uptake in Caco-2 cells. J Pharm Pharmacol 63(12):1522–153010.1111/j.2042-7158.2011.01356.x ↗
7. Kievit FM, Zhang M (2011) Surface engineering of iron oxide nanoparticles for targeted cancer therapy. Acc Chem Res 44(10):853–86210.1021/ar2000277 ↗
8. Kievit FM, Veiseh O, Bhattarai N, Fang C, Gunn JW, Lee D, Ellenbogen RG, Olson JM, Zhang M (2009) PEI-PEG-chitosan copolymer coated iron oxide nanoparticles for safe gene delivery: synthesis, complexation, and transfection. Adv Funct Mater 19(14):2244–225110.1002/adfm.200801844 ↗
9. Ling D, Hyeon T (2012) Chemical design of biocompatible iron oxide nanoparticles for medical applications. Small. doi: 10.1002/smll.20120211110.1002/smll.201202111 ↗
10. Ma M, Zhang Y, Yu W, Shen H, Zhang H, Gu N (2003) Preparation and characterization of magnetite nanoparticles coated by amino silane. Colloid Surf A 212:219–22610.1016/S0927-7757(02)00305-9 ↗
11. Maeda H, Kasuga T, Hench LL (2006) Preparation of poly(l-lactic acid)-polysiloxane-calcium carbonate hybrid membranes for guided bone regeneration. Biomaterials 27(8):1216–122210.1016/j.biomaterials.2005.08.010 ↗
12. Mao X, Cao B, Wood TE, Hurren R, Tong J, Wang X, Wang W, Li J, Jin Y, Sun W, Spagnuolo PA, MacLean N, Moran MF, Datti A, Wrana J, Batey RA, Schimmer AD (2011) A small-molecule inhibitor of d-cyclin transactivation displays preclinical efficacy in myeloma and leukemia via phosphoinositide 3-kinase pathway. Blood 117(6):1986–199710.1182/blood-2010-05-284810 ↗
13. Mehier-Humbert S, Bettinger T, Yan F, Guy RH (2005) Ultrasound-mediated gene delivery: kinetics of plasmid internalization and gene expression. J Control Release 104(1):203–21110.1016/j.jconrel.2005.01.011 ↗
14. Meng Lin M, Kim HH, Kim H, Muhammed M, Kyung Kim D (2010) Iron oxide-based nanomagnets in nanomedicine: fabrication and applications. Nano Rev 1. doi: 10.3402/nano.v1i0.488310.3402/nano.v1i0.4883 ↗
15. Natarajan A, Chun C, Hickman JJ, Molnar P (2008) Growth and electrophysiological properties of rat embryonic cardiomyocytes on hydroxyl- and carboxyl-modified surfaces. J Biomater Sci 19(10):1319–133110.1163/156856208786052399 ↗
16. Nguyen TH, Kim YU, Kim KJ, Choi SS (2009) Investigation of structural transition of dsDNA on various substrates studied by atomic force microscopy. J Nanosci Nanotechnol 9(3):2162–216810.1166/jnn.2009.007 ↗
17. Sahoo SK, Labhasetwar V (2003) Nanotech approaches to drug delivery and imaging. Drug Discov Today 8(24):1112–112010.1016/S1359-6446(03)02903-9 ↗
18. Samal SK, Dash M, Van Vlierberghe S, Kaplan DL, Chiellini E, van Blitterswijk C, Moroni L, Dubruel P (2012) Cationic polymers and their therapeutic potential. Chem Society Res 41(21):7147–719410.1039/c2cs35094g ↗
19. Singh KK (2005) Nanotechnology in cancer detection and treatment. Technol Cancer Res Treat 4(6):58310.1177/153303460500400601 ↗
20. Sun SL, Lo YL, Chen HY, Wang LF (2012) Hybrid polyethylenimine and polyacrylic acid-bound iron oxide as a magnetoplex for gene delivery. Langmuir 28(7):3542–355210.1021/la204529u ↗
21. Tietze R, Lyer S, Durr S, Alexiou C (2012) Nanoparticles for cancer therapy using magnetic forces. Nanomedicine 7(3):447–45710.2217/nnm.12.10 ↗
22. Vergaro V, Abdullayev E, Lvov YM, Zeitoun A, Cingolani R, Rinaldi R, Leporatti S (2010) Cytocompatibility and uptake of halloysite clay nanotubes. Biomacromolecules 11(3):820–82610.1021/bm9014446 ↗
23. Wang S, Wen S, Shen M, Guo R, Cao X, Wang J, Shi X (2011) Aminopropyltriethoxysilane-mediated surface functionalization of hydroxyapatite nanoparticles: synthesis, characterization, and in vitro toxicity assay. Int J Nanomedicine 6:3449–3459
24. Wiekhorst F, Steinhoff U, Eberbeck D, Trahms L (2012) Magnetorelaxometry assisting biomedical applications of magnetic nanoparticles. Pharm Res 29(5):1189–120210.1007/s11095-011-0630-3 ↗
25. Xu L, Kim M, Kim K, Choa Y, Kim H (2009) Surface modified Fe3O4 nanoparticles as a protein delivery vehicle. Colloid Surf A 350:8–1210.1016/j.colsurfa.2009.08.022 ↗