In vivo carcinogenicity study of silver nanoparticles in transgenic rasH2 mice by one single-dose intravenous administration
Source abstract
Abstract
Benefited from their broad-spectrum antimicrobial property, silver nanoparticles (AgNPs) have been widely used in various daily life and medical products. Consequently, the bio-safety of AgNPs, especially long-term in vivo biological effects is of more and more importance. However, there are no correlated publications about AgNP carcinogenicity at present. Thus, in this study, we have investigated the potential carcinogenicity of polyvinylpyrrolidone (PVP)-coated AgNPs with an intermediate size (diameter ~ 42.5 nm). The C57- ras transgenic mouse model (CB6F1 Tg mice) harboring human c-Ha-ras gene was used to shorten the duration of in vivo experiments from 2 years to 22 weeks. CB6F1 Tg mice were intravenously injected by different single doses of AgNPs (0.4, 4, and 20 mg/kg body weight) during the 22-week carcinogenicity study. There were no obvious AgNP-related neoplastic lesions by microscope examination and no increase in the incidence of non-neoplastic lesions in the AgNP-treated mice in our study. Overall, these results indicate that AgNPs (≤ 20 mg/kg) do not cause carcinogenesis in CB6F1 Tg mice via a single-dose intravenous injection. This provides useful toxicological information and is of great importance to more applications of AgNPs in the future.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Nanoparticles: synthesis and applications · Graphene and Nanomaterials Applications · Microplastics and Plastic Pollution
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (53)
Show source-provided references
1. Ahamed M, Alsalhi MS, Siddiqui MK (2010) Silver nanoparticle applications and human health. Clin Chim Acta 411:1841–1848. https://doi.org/10.1016/j.cca.2010.08.01610.1016/j.cca.2010.08.016 ↗
2. Ahlberg S, Antonopulos A, Diendorf J, Dringen R, Epple M, Flöck R, Goedecke W, Graf C, Haberl N, Helmlinger J, Herzog F, Heuer F, Hirn S, Johannes C, Kittler S, Köller M, Korn K, Kreyling WG, Krombach F, Lademann J, Loza K, Luther EM, Malissek M, Meinke MC, Nordmeyer D, Pailliart A, Raabe J, Rancan F, Rothen-Rutishauser B, Rühl E, Schleh C, Seibel A, Sengstock C, Treuel L, Vogt A, Weber K, Zellner R (2014) PVP-coated, negatively charged silver nanoparticles: a multi-center study of their physicochemical characteristics, cell culture and in vivo experiments. Beilstein J Nanotechnol 5:1944–1965. https://doi.org/10.3762/bjnano.5.20510.3762/bjnano.5.205 ↗
3. Asare N, Duale N, Slagsvold HH, Lindeman B, Olsen AK, Gromadzka-Ostrowska J, Meczynska-Wielgosz S, Kruszewski M, Brunborg G, Instanes C (2016) Genotoxicity and gene expression modulation of silver and titanium dioxide nanoparticles in mice. Nanotoxicology 10:312–321. https://doi.org/10.3109/17435390.2015.107144310.3109/17435390.2015.1071443 ↗
4. Beer C, Foldbjerg R, Hayashi Y, Sutherland DS, Autrup H (2012) Toxicity of silver nanoparticles—nanoparticle or silver ion? Toxicol Lett 208:286–292. https://doi.org/10.1016/j.toxlet.2011.11.00210.1016/j.toxlet.2011.11.002 ↗
5. Biju V (2014) Chemical modifications and bioconjugate reactions of nanomaterials for sensing, imaging, drug delivery and therapy. Chem Soc Rev 43:744–764. https://doi.org/10.1039/c3cs60273g10.1039/c3cs60273g ↗
6. Boyle MH, Paranjpe MG, Creasy DM (2018) High background incidence of spontaneous subcapsular adrenal gland hyperplasia of Tg.rasH2 mice used in 26-week carcinogenicity studies. Toxicol Pathol 46:444–448. https://doi.org/10.1177/019262331877096510.1177/0192623318770965 ↗
7. Butler KS, Peeler DJ, Casey BJ, Dair BJ, Elespuru RK (2015) Silver nanoparticles: correlating nanoparticle size and cellular uptake with genotoxicity. Mutagenesis 30:577–591. https://doi.org/10.1093/mutage/gev02010.1093/mutage/gev020 ↗
8. Carotenuto G, Pepe GP, Nicolais L (2000) Preparation and characterization of nano-sized Ag/PVP composites for optical applications. Eur Phys J B 16:11–1710.1007/s100510070243 ↗
9. Chaloupka K, Malam Y, Seifalian AM (2010) Nanosilver as a new generation of nanoproduct in biomedical applications. Trends Biotechnol 28:580–588. https://doi.org/10.1016/j.tibtech.2010.07.00610.1016/j.tibtech.2010.07.006 ↗
10. Che B, Luo Q, Zhai B, Fan G, Liu Z, Cheng K, Xin L (2017) Cytotoxicity and genotoxicity of nanosilver in stable GADD45alpha promoter-driven luciferase reporter HepG2 and A549 cells. Environ Toxicol 32:2203–2211. https://doi.org/10.1002/tox.2243310.1002/tox.22433 ↗
11. Chithrani BD, Ghazani AA, Chan WC (2006) Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. Nano Lett 6:662–668. https://doi.org/10.1021/nl052396o10.1021/nl052396o ↗
12. Danish Consumer Council (2015) The Nanodatabase. http://nanodb.dk/. Accessed 28 Feb 2015
13. Dobrzynska MM, Gajowik A, Radzikowska J, Lankoff A, Dusinska M, Kruszewski M (2014) Genotoxicity of silver and titanium dioxide nanoparticles in bone marrow cells of rats in vivo. Toxicology 315:86–91. https://doi.org/10.1016/j.tox.2013.11.01210.1016/j.tox.2013.11.012 ↗
14. Dziendzikowska K et al (2012) Time-dependent biodistribution and excretion of silver nanoparticles in male Wistar rats. J Appl Toxicol 32:920–928. https://doi.org/10.1002/jat.275810.1002/jat.2758 ↗
15. Environmental Protection Agency of United States (2010) State of the science literature review: everything nanosilver and more scientific, Technical, Research, Engineering and Modeling Support Final Report
16. European Commission (2014) Opinion on nanosilver: safety, health and environmental effects and role in antimicrobial resistance. Scientific Committee on Emerging and Newly Identified Health Risks (SCENIHR)
17. Geranio L, Heuberger M, Nowack B (2009) The behavior of silver nanotextiles during washing. Environ Sci Technol 43:8113–8118. https://doi.org/10.1021/es901833210.1021/es9018332 ↗
18. Gerber C, Lang HP (2006) How the doors to the nanoworld were opened. Nat Nanotechnol 1:3–5. https://doi.org/10.1038/nnano.2006.7010.1038/nnano.2006.70 ↗
19. Gliga AR, Skoglund S, Wallinder IO, Fadeel B, Karlsson HL (2014) Size-dependent cytotoxicity of silver nanoparticles in human lung cells: the role of cellular uptake, agglomeration and Ag release. Part Fibre Toxicol 11:11. https://doi.org/10.1186/1743-8977-11-1110.1186/1743-8977-11-11 ↗
20. Gottschalk F, Sonderer T, Scholz RW, Nowack B (2009) Modeled environmental concentrations of engineered nanomaterials (TiO(2), ZnO, Ag, CNT, fullerenes) for different regions. Environ Sci Technol 43:9216–9222. https://doi.org/10.1021/es901555310.1021/es9015553 ↗
21. Huang Y, Chen S, Bing X, Gao C, Wang T, Yuan B (2011) Nanosilver migrated into food-simulating solutions from commercially available food fresh containers. Packag Technol Sci 24:201–29710.1002/pts.938 ↗
22. International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (1997) ICH Harmonised Tripartite Guideline. Testing for carcinogenicity of pharmaceuticals. S1B wwwichcom
23. Jacobson-Kram D, Sistare FD, Jacobs AC (2004) Use of transgenic mice in carcinogenicity hazard assessment. Toxicol Pathol 32(Suppl 1):49–52. https://doi.org/10.1080/0192623049042476110.1080/01926230490424761 ↗
24. Jiang X, Foldbjerg R, Miclaus T, Wang L, Singh R, Hayashi Y, Sutherland D, Chen C, Autrup H, Beer C (2013) Multi-platform genotoxicity analysis of silver nanoparticles in the model cell line CHO-K1. Toxicol Lett 222:55–63. https://doi.org/10.1016/j.toxlet.2013.07.01110.1016/j.toxlet.2013.07.011 ↗
25. Johnston HJ, Hutchison G, Christensen FM, Peters S, Hankin S, Stone V (2010) A review of the in vivo and in vitro toxicity of silver and gold particulates: particle attributes and biological mechanisms responsible for the observed toxicity. Crit Rev Toxicol 40:328–346. https://doi.org/10.3109/1040844090345307410.3109/10408440903453074 ↗
26. Kawata K, Osawa M, Okabe S (2009) In vitro toxicity of silver nanoparticles at noncytotoxic doses to HepG2 human hepatoma cells. Environ Sci Technol 43:6046–6051. https://doi.org/10.1021/es900754q10.1021/es900754q ↗
27. Kim S, Ryu DY (2013) Silver nanoparticle-induced oxidative stress, genotoxicity and apoptosis in cultured cells and animal tissues. J Appl Toxicol 33:78–89. https://doi.org/10.1002/jat.279210.1002/jat.2792 ↗
28. Kim YJ, Rahman MM, Lee SM, Kim JM, Park K, Kang JH, Seo YR (2019) Assessment of in vivo genotoxicity of citrated-coated silver nanoparticles via transcriptomic analysis of rabbit liver tissue. Int J Nanomedicine 14:393–405. https://doi.org/10.2147/IJN.S17451510.2147/IJN.S174515 ↗
29. Lankveld DP et al (2010) The kinetics of the tissue distribution of silver nanoparticles of different sizes. Biomaterials 31:8350–8361. https://doi.org/10.1016/j.biomaterials.2010.07.04510.1016/j.biomaterials.2010.07.045 ↗
30. Lansdown AB (2010) A pharmacological and toxicological profile of silver as an antimicrobial agent in medical devices. Adv Pharmacol Sci 2010:910686–910616. https://doi.org/10.1155/2010/91068610.1155/2010/910686 ↗
31. Lee BS, Park SJ, Kim YB, Han JS, Jeong EJ, Son HY, Moon KS (2017) Twenty-six-week oral carcinogenicity study of 3-monochloropropane-1,2-diol in CB6F1-rasH2 transgenic mice. Arch Toxicol 91:453–464. https://doi.org/10.1007/s00204-016-1696-910.1007/s00204-016-1696-9 ↗
32. Long GG, Goodman DG, Credille KM, Mann PC, Wilson JM, Cardy R (2010) Hematopoietic proliferative lesions in the spleen of rasH2 transgenic mice treated with MNU. Toxicol Pathol 38:1026–1036. https://doi.org/10.1177/019262331038255710.1177/0192623310382557 ↗
33. Meng J, Ji Y, Liu J, Cheng X, Guo H, Zhang W, Wu X, Xu H (2014) Using gold nanorods core/silver shell nanostructures as model material to probe biodistribution and toxic effects of silver nanoparticles in mice. Nanotoxicology 8:686–696. https://doi.org/10.3109/17435390.2013.82259310.3109/17435390.2013.822593 ↗
34. Moradi-Sardareh H, Basir HRG, Hassan ZM, Davoudi M, Amidi F, Paknejad M (2018) Toxicity of silver nanoparticles on different tissues of Balb/C mice. Life Sci 211:81–90. https://doi.org/10.1016/j.lfs.2018.09.00110.1016/j.lfs.2018.09.001 ↗
35. Nowack B, Krug HF, Height M (2011) 120 years of nanosilver history: implications for policy makers. Environ Sci Technol 45:1177–1183. https://doi.org/10.1021/es103316q10.1021/es103316q ↗
36. Paranjpe MG, Shah SA, Denton MD, Elbekai RH (2013) Incidence of spontaneous non-neoplastic lesions in transgenic CBYB6F1-Tg (HRAS)2Jic mice. Toxicol Pathol 41:1137–1145. https://doi.org/10.1177/019262331347820710.1177/0192623313478207 ↗
37. Pritchard JB, French JE, Davis BJ, Haseman JK (2003) The role of transgenic mouse models in carcinogen identification. Environ Health Perspect 111:444–454. https://doi.org/10.1289/ehp.577810.1289/ehp.5778 ↗
38. Pulit-Prociak J, Banach M (2016) Silver nanoparticles—a material of the future … ? Open Chem 14:76–9110.1515/chem-2016-0005 ↗
39. Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27:76–83. https://doi.org/10.1016/j.biotechadv.2008.09.00210.1016/j.biotechadv.2008.09.002 ↗
40. Recordati C, de Maglie M, Bianchessi S, Argentiere S, Cella C, Mattiello S, Cubadda F, Aureli F, D’Amato M, Raggi A, Lenardi C, Milani P, Scanziani E (2016) Tissue distribution and acute toxicity of silver after single intravenous administration in mice: nano-specific and size-dependent effects. Part Fibre Toxicol 13:12. https://doi.org/10.1186/s12989-016-0124-x10.1186/s12989-016-0124-x ↗
41. Robinson DE, MacDonald JS (2001) Background and framework for ILSI’s collaborative evaluation program on alternative models for carcinogenicity assessment. International Life Sciences Institute. Toxicol Pathol 29(Suppl):13–19. https://doi.org/10.1080/01926230175317843810.1080/019262301753178438 ↗
42. Roda E, Barni S, Milzani A, Dalle-Donne I, Colombo G, Coccini T (2017) Single silver nanoparticle instillation induced early and persisting moderate cortical damage in rat kidneys. Int J Mol Sci 18. https://doi.org/10.3390/ijms1810211510.3390/ijms18102115 ↗
43. Singh N, Manshian B, Jenkins GJS, Griffiths SM, Williams PM, Maffeis TGG, Wright CJ, Doak SH (2009) NanoGenotoxicology: the DNA damaging potential of engineered nanomaterials. Biomaterials 30:3891–3914. https://doi.org/10.1016/j.biomaterials.2009.04.00910.1016/j.biomaterials.2009.04.009 ↗
44. Suemizu H, Muguruma K, Maruyama C, Tomisawa M, Kimura M, Hioki K, Shimozawa N, Ohnishi Y, Tamaoki N, Nomura T (2002) Transgene stability and features of rasH2 mice as an animal model for short-term carcinogenicity testing. Mol Carcinog 34:1–9. https://doi.org/10.1002/mc.1004510.1002/mc.10045 ↗
45. Takaoka M, Sehata S, Maejima T, Imai T, Tor M, Satoh H, Toyosawa K, Tanakamaru ZY, Adachi T, Hisada S, Ueda M, Ogasawara H, Matsumoto M, Kobayashi K, Mutai M, Usui T (2003) Interlaboratory comparison of short-term carcinogenicity studies using CB6F1-rasH2 transgenic mice. Toxicol Pathol 31:191–199. https://doi.org/10.1080/0192623039018367010.1080/01926230390183670 ↗
46. Tamaoki N (2001) The rasH2 transgenic mouse: nature of the model and mechanistic studies on tumorigenesis. Toxicol Pathol 29(Suppl):81–89. https://doi.org/10.1080/01926230175317849210.1080/019262301753178492 ↗
47. Tang J, Xiong L, Wang S, Wang J, Liu L, Li J, Yuan F, Xi T (2009) Distribution, translocation and accumulation of silver nanoparticles in rats. J Nanosci Nanotechnol 9:4924–483210.1166/jnn.2009.1269 ↗
48. Tiwari R, Singh RD, Khan H, Gangopadhyay S, Mittal S, Singh V, Arjaria N, Shankar J, Roy SK, Singh D, Srivastava V (2017) Oral subchronic exposure to silver nanoparticles causes renal damage through apoptotic impairment and necrotic cell death. Nanotoxicology 11:671–686. https://doi.org/10.1080/17435390.2017.134387410.1080/17435390.2017.1343874 ↗
49. Usui T, Mutai M, Hisada S, Takoaka M, Soper KA, McCullough B, Alden C (2001) CB6F1-rasH2 mouse: overview of available data. Toxicol Pathol 29(Suppl):90–108. https://doi.org/10.1080/01926230175317850010.1080/019262301753178500 ↗
50. Wu M, Chen L, Li R, Dan M, Liu H, Wang X, Wu X, Liu Y, Xu L, Xie L (2018) Bio-distribution and bio-availability of silver and gold in rat tissues with silver/gold nanorod administration. RSC Adv 8:12260–1226810.1039/C8RA00044A ↗
51. Wu M, Guo H, Liu L, Liu Y, Xie L (2019) Size-dependent cellular uptake and localization profiles of silver nanoparticles. Int J Nanomedicine 14:4247–4259. https://doi.org/10.2147/IJN.S20110710.2147/IJN.S201107 ↗
52. Xue Y, Zhang S, Huang Y, Zhang T, Liu X, Hu Y, Zhang Z, Tang M (2012) Acute toxic effects and gender-related biokinetics of silver nanoparticles following an intravenous injection in mice. J Appl Toxicol 32:890–899. https://doi.org/10.1002/jat.274210.1002/jat.2742 ↗
53. Yang L, Kuang H, Zhang W, Aguilar ZP, Wei H, Xu H (2017) Comparisons of the biodistribution and toxicological examinations after repeated intravenous administration of silver and gold nanoparticles in mice. Sci Rep 7:3303. https://doi.org/10.1038/s41598-017-03015-110.1038/s41598-017-03015-1 ↗