In vitro cytotoxicity and genotoxicity studies of gold nanoparticles-mediated photo-thermal therapy versus 5-fluorouracil
Source abstract
Abstract
This study evaluates tumour cell-killing efficacy of metallic gold nanoparticles (AuNPs)-mediated photo-thermal therapy (PTT) in comparison to 5-fluorouracil (5-FU) as a standard chemotherapeutic drug. It also focuses on the possible genetic abnormalities of both drugs in normal blood lymphocytes. Both 5-FU and light-activated spherical AuNPs of 15± nm diameter were used to target MCF-7 breast cancer cell line. Alkaline comet assay, standard karyotyping and multiplex fluorescent in situ hybridization were applied in order to investigate the respective possible genotoxic and mutagenic side effects that might result from the application of each therapeutic modality. Results showed that the LC25 of AuNPs-mediated PTT was achieved at a concentration of 100 µM for 12-h incubation and exposure to light energy of 50 J/cm2, while the same cytotoxic effect was obtained by incubating the MCF-7 cells with the same concentration of the chemotherapeutic drug 5-FU for 24 h. On the other hand, AuNPs showed insignificant genotoxic effect of DNA damage represented by 4.6 % in comparison to 18.58 % exerted by 5-FU. The chromosomal studies resulted in normal karyotypes for cells treated with AuNPs-mediated PTT, while those treated with 5-FU showed several types of numerical as well as structural chromosomal aberrations. In conclusion, compared to 5-FU, light-activated AuNPs-mediated PTT provides considerable efficacy in breast cancer cells killing with no genetic side effects under the proposed experimental conditions.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Photodynamic Therapy Research Studies · Nanoplatforms for cancer theranostics · Gold and Silver Nanoparticles Synthesis and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (34)
Show source-provided references
1. Ahn BZ, Yoon YD, Lee YH, Kim BH, Sok DE (1998) Inhibitory effect of bupleuri radix saponins on adhesion of some solid tumor cells and relation to hemolytic action: screening of 232 herbal drugs for anti-cell adhesion. Planta Med 64:220–22410.1055/s-2006-957413 ↗
2. Anderson D, Plewa MJ (1998) The international comet assay workshop. Mutagenesis 13(1):67–7310.1093/mutage/13.1.67 ↗
3. Bhattacharya R, Patra CR, Verma R et al (2006) Gold nanoparticles inhibit the proliferation of multiple myeloma cells. Adv Mater 5:711–716
4. Bray F, McCarron P, Parkin DM (2004) The changing global patterns of female breast cancer incidence and mortality. Breast Cancer Res 6:229–23910.1186/bcr932 ↗
5. Cai W, Gao T, Hong H et al (2008) Applications of gold nanoparticles in cancer nanotechnology. Nanotechnol Sci Appl 1:17–3210.2147/NSA.S3788 ↗
6. Choi YJ, Kim YJ, Lee JW et al (2013) Cytotoxicity and genotoxicity induced by photothermal effects of colloidal gold nanorods. J Nanosci Nanotechnol 13:4437–444510.1166/jnn.2013.7176 ↗
7. El-Sayed IH, Huang X, El-Sayed MA (2006) Selective laser photo-thermal therapy of epithelial carcinoma using anti-EGFR antibody conjugated gold nanoparticles. Cancer Lett 239:129–13510.1016/j.canlet.2005.07.035 ↗
8. Ferrari M (2005) Cancer nanotechnology: opportunities and challenges. Nat Rev Cancer 5:161–17110.1038/nrc1566 ↗
9. Gannon CJ, Patra CR, Bhattacharya R et al (2008) Intracellular gold nanoparticles enhance non-invasive radiofrequency thermal destruction of human gastrointestinal cancer cells. J Nanobiotechnol 6(2):1–9
10. Han R, Yang YM, Dietrich J et al (2008) Systemic 5-fluorouracil treatment causes a syndrome of delayed myelin destruction in the central nervous system. J Biol 7:1210.1186/jbiol69 ↗
11. Hartmann A, Plappert U, Poetter F et al (2003) Comparative study with the alkaline comet assay and the chromosome aberration test. Mut Res 536(1–2):27–3810.1016/S1383-5718(03)00035-4 ↗
12. Henderson L, Wolfreys A, Fedyk J et al (1998) The ability of the Comet assay to discriminate between genotoxins and cytotoxins. Mutagenesis 13(1):89–9410.1093/mutage/13.1.89 ↗
13. Huang X, Jain PK, El-Sayed IH et al (2006) Determination of the minimum temperature required for selective photothermal destruction of cancer cells with the use of immunotargeted gold nanoparticles. Photochem Photobiol 82:412–41710.1562/2005-12-14-RA-754 ↗
14. Klein K, Godnić-Cvar J (2012) Genotoxicity of metal nanoparticles: focus on in vivo studies. Arh Hig Rada Toksikol 63:133–14510.2478/10004-1254-63-2012-2213 ↗
15. Kopjar N, Milas I, Garaj-Vrhovac V et al (2007) Cytogenetic outcomes of adjuvant chemotherapy in non-target cells of breast cancer patients. Hum Exp Toxicol 26:391–39910.1177/0960327106076812 ↗
16. Kuechler A, Neubauer S, Grabenbauer GG et al (2002) Is 24-color FISH detection of in vitro radiation-induced chromosomal aberrations suited to determine individual intrinsic radiosensitivity? Strahlenther Onkol 178(4):209–21510.1007/s00066-002-0904-0 ↗
17. Liehr T, Thoma K, Kammler K et al (1995) Direct preparation of uncultured EDTA-treated or heparinized blood for Interphase FISH analysis. Appl Cytogenet 21(6):185–188
18. Liu Z, Cai W, He L et al (2007) In vivo biodistribution and highly efficient tumour targeting of carbon nanotubes in mice. Nat Nanotechnol 22:47–5210.1038/nnano.2006.170 ↗
19. Paino IMM, Marangoni VS, de Oliveira RDCS, Antunes LMG, Zucolotto V (2012) Cyto and genotoxicity of gold nanoparticles in human hepatocellular carcinoma and peripheral blood mononuclear cells. Toxicol Lett 215(2):119–12510.1016/j.toxlet.2012.09.025 ↗
20. Park JW, Benz CC, Martin FJ (2004) Future directions of liposome- and immunoliposome-based cancer therapeutics. Semin Oncol 31:196–20510.1053/j.seminoncol.2004.08.009 ↗
21. Patlolla AK, Epting T, Thrasher L, Tchounwou PB (2014) Cytotoxic and genotoxic effects of PEG coated gold nanoparticles in HK-2 cells. Cancer Res 74(19 Supplement):228410.1158/1538-7445.AM2014-2284 ↗
22. Qian W, Huang X, Kang B, El-Sayed M (2010) Dark field light scattering imaging of living cancer cell component from birth through division using bioconjugated gold nanoprobes. J Biomed Opt 15(4):04602510.1117/1.3477179 ↗
23. Raposa T, Varkonyi J (1987) The relationship between sister chromatid exchange induction and leukemogenicity of different cytostatics. Cancer Detect Prev 10:141–151
24. Scheper RJ, Vos A, de Groot J et al (1984) Evaluation of various cytostatic drugs as local immunotherapeutic agents. Invest New Drugs 2:221–22510.1007/BF00232355 ↗
25. Singh NP, McCoy MT, Tice RR et al (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Cell Res 175(1):184–19110.1016/0014-4827(88)90265-0 ↗
26. Singh N, Manshian B, Jenkins GJ et al (2009) NanoGenotoxicology: the DNA damaging potential of engineered nanomaterials. Biomaterials 30:3891–391410.1016/j.biomaterials.2009.04.009 ↗
27. Stone V, Donaldson K (2006) Nanotoxicology - signs of stress. Nat Nanotechnol 1:23–2410.1038/nnano.2006.69 ↗
28. Teixeira L, Barry S, Debourdeau P et al (2004) Cardiotoxicity of 5-fluorouracil. Bull Cancer 91:154–158
29. Thorek DL, Chen AK, Czupryna J et al (2006) Superparamagnetic iron oxide nanoparticle probes for molecular imaging. Ann Biomed Eng 34:23–3810.1007/s10439-005-9002-7 ↗
30. Wehler TC, Hamdi S, Maderer A et al (2013) Single-agent therapy with sorafenib or 5-FU is equally effective in human colorectal cancer xenograft–no benefit of combination therapy. Int J Colorectal Dis 28(3):385–39810.1007/s00384-012-1551-2 ↗
31. Wei XL, Mo ZH, Li B et al (2007) Disruption of HepG2 cell adhesion by gold nanoparticle and Paclitaxel disclosed by in situ QCM measurement. Colloids Surf B Biointerfaces 59:100–10410.1016/j.colsurfb.2007.04.016 ↗
32. Yah CS (2013) The toxicity of gold nanoparticles in relation to their physiochemical properties. Biomed Res 24:400–413
33. Yoshikawa R, Kusunoki M, Yanagi H et al (2001) Hashimoto-Tamaoki, Dual antitumor effects of 5-fluorouracil on the cell cycle in colorectal carcinoma cells: a novel target mechanism concept for pharmacokinetic modulating chemotherapy. Cancer Res 61:1029–1037
34. Zheng Y, Hunting DJ, Ayotte P et al (2008) Radiosensitization of DNA by gold nanoparticles irradiated with high-energy electrons. Radiat Res 169:19–2710.1667/RR1080.1 ↗