PEGylation of SPIONs by polycondensation reactions: a new strategy to improve colloidal stability in biological media
Source abstract
Abstract
In this study, we report on a new route of PEGylation of superparamagnetic iron oxide nanoparticles (SPIONs) by polycondensation reaction with carboxylate groups. Structural and magnetic characterizations were performed by X-ray diffractometry (XRD), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and vibrating sample magnetometry (VSM). The XRD confirmed the spinel structure with a crystallite average diameter in the range of 3.5–4.1 nm in good agreement with the average diameter obtained by TEM (4.60–4.97 nm). The TGA data indicate the presence of PEG attached onto the SPIONs’ surface. The SPIONs were superparamagnetic at room temperature with saturation magnetization (M S) from 36.7 to 54.1 emu/g. The colloidal stability of citrate- and PEG-coated SPIONs was evaluated by means of dynamic light scattering measurements as a function of pH, ionic strength, and nature of dispersion media (phosphate buffer and cell culture media). Our findings demonstrated that the PEG polymer chain length plays a key role in the coagulation behavior of the Mag-PEG suspensions. The excellent colloidal stability under the extreme conditions we evaluated, such as high ionic strength, pH near the isoelectric point, and cell culture media, revealed that suspensions comprising PEG-coated SPION, with PEG of molecular weight 600 and above, present steric stabilization attributed to the polymer chains attached onto the surface of SPIONs.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanoparticle-Based Drug Delivery · Characterization and Applications of Magnetic Nanoparticles · Iron oxide chemistry and applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (62)
Show source-provided references
1. Albornoz C, Jacobo SE (2006) Preparation of a biocompatible magnetic film from an aqueous ferrofluid. J Magn Magn Mater 305(1):12–15. doi: 10.1016/j.jmmm.2005.11.02110.1016/j.jmmm.2005.11.021 ↗
2. Amici J, Celasco E, Allia P, Tiberto P, Sangermano M (2011) Poly(ethylene glycol)-coated magnetite nanoparticles: preparation and characterization. Macromol Chem Phys 212(4):411–416. doi: 10.1002/macp.20100070710.1002/macp.201000707 ↗
3. Amstad E, Gillich T, Bilecka I, Textor M, Reimhult E (2009) Ultrastable iron oxide nanoparticle colloidal suspensions using dispersants with catechol-derived anchor groups. Nano Lett 9(12):4042–4048. doi: 10.1021/Nl902212q10.1021/nl902212q ↗
4. Barrera C, Herrera AP, Bezares N, Fachini E, Olayo-Valles R, Hinestroza JP, Rinaldi C (2012) Effect of poly(ethylene oxide)-silane graft molecular weight on the colloidal properties of iron oxide nanoparticles for biomedical applications. J Colloid Interf Sci 377:40–50. doi: 10.1016/j.jcis.2012.03.05010.1016/j.jcis.2012.03.050 ↗
5. Bitea C, Walther C, Kim JI, Geckeis H, Rabung T, Scherbaum FJ, Cacuci DG (2003) Time-resolved observation of ZrO2-colloid agglomeration. Colloid Surface A 215(1–3):55–66. doi: 10.1016/S0927-7757(02)00415-610.1016/S0927-7757(02)00415-6 ↗
6. Butterworth MD, Illum L, Davis SS (2001) Preparation of ultrafine silica- and PEG-coated magnetite particles. Colloid Surface A 179(1):93–102. doi: 10.1016/S0927-7757(00)00633-610.1016/S0927-7757(00)00633-6 ↗
7. Cabuil V, Massart R, Bacri JC, Perzynski R, Salin D (1987) Ionic ferrofluids—toward fractional distillation. J Chem Res-S 5:130–131
8. Chatterjee J, Haik Y, Chen CJ (2003) Size dependent magnetic properties of iron oxide nanoparticles. J Magn Magn Mater 257(1):113–118. doi: 10.1016/S0304-8853(02)01066-110.1016/S0304-8853(02)01066-1 ↗
9. Chin AB, Yaacob II (2007) Synthesis and characterization of magnetic iron oxide nanoparticles via w/o microemulsion and Massart’s procedure. J Mater Process Tech 191(1–3):235–237. doi: 10.1016/j.jmatprotec.2007.03.01110.1016/j.jmatprotec.2007.03.011 ↗
10. Cordente N, Respaud M, Senocq F, Casanove MJ, Amiens C, Chaudret B (2001) Synthesis and magnetic properties of nickel nanorods. Nano Lett 1(10):565–568. doi: 10.1021/Nl010052210.1021/nl0100522 ↗
11. Cornell RM, Schwertmann U (2004) Surface chemistry and colloidal stability. In: The iron oxides. Wiley Weinheim, pp 221–252
12. Cullity BD (1978) Elements of X-ray diffraction 2nd edn. Addison-Wesley, Reading
13. Cullity BD, Graham CD (2009) Introduction to magnetic materials. IEEE Press, Piscataway
14. Dave SR, Gao XH (2009) Monodisperse magnetic nanoparticles for biodetection, imaging, and drug delivery: a versatile and evolving technology. Wires Nanomed Nanobi 1(6):583–609. doi: 10.1002/Wnan.5110.1002/wnan.51 ↗
15. Deng XM, Liu Y, Yuan ML (2002) Study on biodegradable polymer. 3. Synthesis and characterization of poly(DL-lactic acid)-co-poly(ethylene glycol)-co-poly(l-lysine) copolymer. Eur Polym J 38(7):1435–1441. doi: 10.1016/S0014-3057(02)00017-410.1016/S0014-3057(02)00017-4 ↗
16. Di Marco M, Guilbert I, Port M, Robic C, Couvreur P, Dubernet C (2007) Colloidal stability of ultrasmall superparamagnetic iron oxide (USPIO) particles with different coatings. Int J Pharm 331(2):197–203. doi: 10.1016/j.ijpharm.2006.11.00210.1016/j.ijpharm.2006.11.002 ↗
17. Ding T, Liu QY, Shi R, Tian M, Yang H, Zhang LQ (2006) Synthesis, characterization and in vitro degradation study of a novel and rapidly degradable elastomer. Polym Degrad Stabil 91(4):733–739. doi: 10.1016/j.polymdegradstab.2005.06.00710.1016/j.polymdegradstab.2005.06.007 ↗
18. Fauconnier N, Bee A, Roger J, Pons JN (1996) Adsorption of gluconic and citric acids on maghemite particles in aqueous medium. Prog Coll Pol Sci S 100:212–21610.1007/BFb0115782 ↗
19. Goya GF, Berquo TS, Fonseca FC, Morales MP (2003) Static and dynamic magnetic properties of spherical magnetite nanoparticles. J Appl Phys 94(5):3520–3528. doi: 10.1063/1.159995910.1063/1.1599959 ↗
20. Gubin SP, Spichkin YI, Koksharov YA, Yurkov GY, Kozinkin AV, Nedoseikina TI, Korobov MS, Tishin AM (2003) Magnetic and structural properties of Co nanoparticles in a polymeric matrix. J Magn Magn Mater 265(2):234–242. doi: 10.1016/S0304-8853(03)00271-310.1016/S0304-8853(03)00271-3 ↗
21. Gupta AK, Gupta M (2005) Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials 26(18):3995–4021. doi: 10.1016/j.biomaterials.2004.10.01210.1016/j.biomaterials.2004.10.012 ↗
22. Gyawali D, Nair P, Zhang Y, Tran RT, Zhang C, Samchukov M, Makarov M, Kim HKW, Yang JA (2010) Citric acid-derived in situ crosslinkable biodegradable polymers for cell delivery. Biomaterials 31(34):9092–9105. doi: 10.1016/j.biomaterials.2010.08.02210.1016/j.biomaterials.2010.08.022 ↗
23. Hiemenz P, Rajagopalan R (1997) Principles of colloid and surface chemistry. Marcell Dekker, New York10.1201/9781315274287 ↗
24. Hou YL, Kondoh H, Kogure T, Ohta T (2004) Preparation and characterization of monodisperse FePd nanoparticles. Chem Mater 16(24):5149–5152. doi: 10.1021/Cm048902c10.1021/cm048902c ↗
25. Hunter RJ (1981) Zeta potential in colloid science principles and applications. Academic Press, London
26. Hyeon T, Lee SS, Park J, Chung Y, Bin Na H (2001) Synthesis of highly crystalline and monodisperse maghemite nanocrystallites without a size-selection process. J Am Chem Soc 123(51):12798–12801. doi: 10.1021/Ja016812s10.1021/ja016812s ↗
27. Kamat PV (2002) Photophysical, photochemical and photocatalytic aspects of metal nanoparticles. J Phys Chem B 106(32):7729–7744. doi: 10.1021/Jp020928910.1021/jp0209289 ↗
28. Kodama RH (1999) Magnetic nanoparticles. J Magn Magn Mater 200(1–3):359–372. doi: 10.1016/S0304-8853(99)00347-910.1016/S0304-8853(99)00347-9 ↗
29. Laurent S, Forge D, Port M, Roch A, Robic C, Elst LV, Muller RN (2008) Magnetic iron oxide nanoparticles: synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications. Chem Rev 108(6):2064–2110. doi: 10.1021/Cr068445e10.1021/cr068445e ↗
30. Lee JW, Isobe T, Senna M (1996) Magnetic properties of ultrafine magnetite particles and their slurries prepared via in-situ precipitation. Colloid Surface A 109:121–12710.1016/0927-7757(95)03479-X ↗
31. Lekkerkerker H, Tuinier R (2011) Colloids and the depletion interaction. Springer, New York10.1007/978-94-007-1223-2 ↗
32. Leslie-Pelecky DL, Rieke RD (1996) Magnetic properties of nanostructured materials. Chem Mater 8(8):1770–1783. doi: 10.1021/Cm960077f10.1021/cm960077f ↗
33. Lu AH, Schmidt W, Matoussevitch N, Bonnemann H, Spliethoff B, Tesche B, Bill E, Kiefer W, Schuth F (2004) Nanoengineering of a magnetically separable hydrogenation catalyst. Angew Chem Int Edit 43(33):4303–4306. doi: 10.1002/anie.20045422210.1002/anie.200454222 ↗
34. Lu AH, Salabas EL, Schuth F (2007) Magnetic nanoparticles: synthesis, protection, functionalization, and application. Angew Chem Int Edit 46(8):1222–1244. doi: 10.1002/anie.20060286610.1002/anie.200602866 ↗
35. Lu CC, Bhatt LR, Jun HY, Park SH, Chai KY (2012) Carboxyl-polyethylene glycol-phosphoric acid: a ligand for highly stabilized iron oxide nanoparticles. J Mater Chem 22(37):19806–19811. doi: 10.1039/C2jm34327d10.1039/c2jm34327d ↗
36. Lutz JF, Stiller S, Hoth A, Kaufner L, Pison U, Cartier R (2006) One-pot synthesis of PEGylated ultrasmall iron-oxide nanoparticles and their in vivo evaluation as magnetic resonance imaging contrast agents. Biomacromolecules 7(11):3132–3138. doi: 10.1021/Bm060752710.1021/bm0607527 ↗
37. Massart R (1981) Preparation of aqueous magnetic liquids in alkaline and acidic media. IEEE T Magn 17(2):1247–1248. doi: 10.1109/Tmag.1981.106118810.1109/TMAG.1981.1061188 ↗
38. Morais PC, Garg VK, Oliveira AC, Silva LP, Azevedo RB, Silva AML, Lima ECD (2001) Synthesis and characterization of size-controlled cobalt-ferrite-based ionic ferrofluids. J Magn Magn Mater 225(1–2):37–40. doi: 10.1016/S0304-8853(00)01225-710.1016/S0304-8853(00)01225-7 ↗
39. Mukhopadhyay A, Joshi N, Chattopadhyay K, De G (2012) A facile synthesis of PEG-coated magnetite (Fe3O4) nanoparticles and their prevention of the reduction of cytochrome C. Acs Appl Mater Inter 4(1):142–149. doi: 10.1021/Am201166m10.1021/am201166m ↗
40. Nakamoto K (1970) Infrared spectra of inorganic and coordination compounds, 4th edn. Wiley, New York
41. Ni F, Jiang L, Yang RX, Chen ZP, Qi X, Wang JW (2012) Effects of PEG length and iron oxide nanoparticles size on reduced protein adsorption and non-specific uptake by macrophage cells. J Nanosci Nanotechno 12(3):2094–2100. doi: 10.1166/jnn.2012.575310.1166/jnn.2012.5753 ↗
42. Odenbach S (2003) Magnetic fluids—suspensions of magnetic dipoles and their magnetic control. J Phys-Condens Mat 15(15):S1497–S1508. doi: 10.1088/0953-8984/15/15/31210.1088/0953-8984/15/15/312 ↗
43. Otsuka H, Nagasaki Y, Kataoka K (2003) PEGylated nanoparticles for biological and pharmaceutical applications. Adv Drug Deliver Rev 55(3):403–419. doi: 10.1016/S0169-409x(02)00226-010.1016/S0169-409X(02)00226-0 ↗
44. Pankhurst QA, Connolly J, Jones SK, Dobson J (2003) Applications of magnetic nanoparticles in biomedicine. J Phys D Appl Phys 36(13):R167–R181. doi: 10.1088/0022-3727/36/13/20110.1088/0022-3727/36/13/201 ↗
45. Park JY, Daksha P, Lee GH, Woo S, Chang YM (2008) Highly water-dispersible PEG surface modified ultra small superparamagnetic iron oxide nanoparticles useful for target-specific biomedical applications. Nanotechnology 19(36):365603. doi: 10.1088/0957-4484/19/36/36560310.1088/0957-4484/19/36/365603 ↗
46. Pasche S, Voros J, Griesser HJ, Spencer ND, Textor M (2005) Effects of ionic strength and surface charge on protein adsorption at PEGylated surfaces. J Phys Chem B 109(37):17545–17552. doi: 10.1021/Jp050431+10.1021/jp050431+ ↗
47. Popplewell J, Sakhnini L (1995) The dependence of the physical and magnetic-properties of magnetic fluids on particle-size. J Magn Magn Mater 149(1–2):72–78. doi: 10.1016/0304-8853(95)00341-X10.1016/0304-8853(95)00341-X ↗
48. Quinzler D, Mecking S (2009) Renewable resource-based poly(dodecyloate) by carbonylation polymerization. Chem Commun 36:5400–5402. doi: 10.1039/B912294j10.1039/b912294j ↗
49. Richter AW, Akerblom E (1983) Antibodies against polyethylene-glycol produced in animals by immunization with monomethoxy polyethylene-glycol modified proteins. Int Arch Aller a Imm 70(2):124–13110.1159/000233309 ↗
50. Roca AG, Costo R, Rebolledo AF, Veintemillas-Verdaguer S, Tartaj P, Gonzalez-Carreno T, Morales MP, Serna CJ (2009) Progress in the preparation of magnetic nanoparticles for applications in biomedicine. J Phys D Appl Phys 42 (22). doi: 10.1088/0022-3727/42/22/22400210.1088/0022-3727/42/22/224002 ↗
51. Sampaio JF, Beverly KC, Heath JR (2001) DC transport in self-assembled 2D layers of Ag nanoparticles. J Phys Chem B 105(37):8797–880010.1021/jp010870i ↗
52. Schudel M, Behrens SH, Holthoff H, Kretzschmar R, Borkovec M (1997) Absolute aggregation rate constants of hematite particles in aqueous suspensions: a comparison of two different surface morphologies. J Colloid Interf Sci 196(2):241–253. doi: 10.1006/jcis.1997.520710.1006/jcis.1997.5207 ↗
53. Soler MAG, Lima ECD, Nunes ES, Silva FLR, Oliveira AC, Azevedo RB, Morais PC (2011) Spectroscopic study of maghemite nanoparticles surface-grafted with DMSA. J Phys Chem A 115(6):1003–1008. doi: 10.1021/jp110991610.1021/jp1109916 ↗
54. Sun SH, Murray CB (1999) Synthesis of monodisperse cobalt nanocrystals and their assembly into magnetic superlattices (invited). J Appl Phys 85(8):4325–4330. doi: 10.1063/1.37035710.1063/1.370357 ↗
55. Tartaj P, Morales MD, Veintemillas-Verdaguer S, Gonzalez-Carreno T, Serna CJ (2003) The preparation of magnetic nanoparticles for applications in biomedicine. J Phys D Appl Phys 36(13):R182–R197. doi: 10.1088/0022-3727/36/13/20210.1088/0022-3727/36/13/202 ↗
56. Unsoy G, Yalcin S, Khodadust R, Gunduz G, Gunduz U (2012) Synthesis optimization and characterization of chitosan-coated iron oxide nanoparticles produced for biomedical applications. J Nanopart Res 14 (11). doi: 10.1007/S11051-012-0964-810.1007/S11051-012-0964-8 ↗
57. Vayssieres L (2005) On the thermodynamic stability of metal oxide nanoparticles in aqueous solutions. Int J Nanotechnol 2(4):411–439. doi: 10.1504/Ijnt.2005.00807710.1504/IJNT.2005.008077 ↗
58. Vayssieres L, Chaneac C, Tronc E, Jolivet JP (1998) Size tailoring of magnetite particles formed by aqueous precipitation: an example of thermodynamic stability of nanometric oxide particles. J Colloid Interf Sci 205(2):205–212. doi: 10.1006/jcis.1998.561410.1006/jcis.1998.5614 ↗
59. Viali WR, Alcantara GB, Sartoratto PPC, Soler MAG, Mosiniewicz-Szablewska E, Andrzejewski B, Morais PC (2010) Investigation of the molecular surface coating on the stability of insulating magnetic oils. J Phys Chem C 114(1):179–188. doi: 10.1021/Jp908732b10.1021/jp908732b ↗
60. Wan JX, Chen XY, Wang ZH, Yang XG, Qian YT (2005) A soft-template-assisted hydrothermal approach to single-crystal Fe3O4 nanorods. J Cryst Growth 276(3–4):571–576. doi: 10.1016/j.jcrysgro.2004.11.42310.1016/j.jcrysgro.2004.11.423 ↗
61. Wu W, He QG, Jiang CZ (2008) Magnetic iron oxide nanoparticles: synthesis and surface functionalization strategies. Nanoscale Res Lett 3(11):397–415. doi: 10.1007/s11671-008-9174-910.1007/s11671-008-9174-9 ↗
62. Zhang MQ, Desai T, Ferrari M (1998) Proteins and cells on PEG immobilized silicon surfaces. Biomaterials 19(10):953–96010.1016/S0142-9612(98)00026-X ↗