Synthesis of Ni–Zn ferrite nanoparticles in radiofrequency thermal plasma reactor and their use for purification of histidine-tagged proteins
Source abstract
Abstract
Superparamagnetic Ni–Zn ferrite nanoparticles were synthesized in radiofrequency thermal plasma reactor from aqueous solutions of Ni- and Zn-nitrates. The nanoparticles were studied for protein purification performance in both quantitative and qualitative terms. For comparison, experiments were also performed by Ni-charged affinity chromatography. It was proved that the Ni–Zn ferrite nanoparticles effectively purified histidine-tagged proteins with a maximum protein binding capacity of about 7% (w/w). Gel electrophoresis demonstrated better purification characteristics for magnetic nanoparticles than for affinity chromatography.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Protein purification and stability · Nanoparticle-Based Drug Delivery · Enzyme Structure and Function
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (13)
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