Near-infrared (NIR-II) fluorescent poly(ethylene terephthalate) nano-microplastics for in vivo tracking
Source abstract
Abstract
Abstract Environmental health concerns because of nano-microplastics (NMPs) are growing, yet in vivo data in mammals remain scarce. Most analysis techniques rely on excised organs, limiting the ability to observe real-time NMP migration within living organisms. Therefore, there is an increasing need for simple, non-invasive techniques to track NMPs in vivo. In this study, we utilized near-infrared (NIR) fluorescence (NIRF), known as the second biological window in NIR (NIR-II), and applied it to in vivo optical imaging of deep tissue. NIR-II fluorescent poly(ethylene terephthalate) (PET) particles were synthesized by encapsulating the NIR-II fluorescent dye IR-1061 into PET. PET was dissolved in acetonitrile and stirred with IR-1061 under a nitrogen atmosphere for 24 h, with a dye loading of 1.56 mg/mg PET. To prevent agglomeration and control particle size (30–300 nm), an aqueous bovine serum albumin solution (0.02–50 mg/mL) was added. The mixture was then stirred in open air for 48 h to evaporate residual acetonitrile. In vivo NIRF imaging enabled real-time tracking of PET particles’ distribution over time following oral administration to mice. This method offers a promising platform for evaluating the behavior and potential toxicity of NMPs based on their size and composition.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Microplastics and Plastic Pollution · Nanocomposite Films for Food Packaging · biodegradable polymer synthesis and properties
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (38)
Show source-provided references
1. García JM (2016) Catalyst: design challenges for the future of plastic recycling. Chem 1(6):813–815. https://doi.org/10.1016/j.chempr.2016.11.00310.1016/j.chempr.2016.11.003 ↗
2. Cox KD, Covernton GA, Davies HL, Dower JF, Juanes F, Dudas SE (2019) Human consumption of microplastics. Environ Sci Technol 53(12):7068–7074. https://doi.org/10.1021/acs.est.9b0151710.1021/acs.est.9b01517 ↗
3. Zubris KAV, Richards BK (2005) Synthetic fibers as an indicator of land application of sludge. Environ Pollut 138(2):201–211. https://doi.org/10.1016/j.envpol.2005.04.01310.1016/j.envpol.2005.04.013 ↗
4. Eerkes-Medrano D, Thompson RC, Aldridge DC (2015) Microplastics in freshwater systems: a review of the emerging threats, identification of knowledge gaps, and prioritization of research needs. Water Res 75:63–82. https://doi.org/10.1016/j.watres.2015.02.01210.1016/j.watres.2015.02.012 ↗
5. Boucher J, Friot D (2017) Primary microplastics in the oceans: a global evaluation of sources. International Union for Conservation of Nature. https://doi.org/10.2305/IUCN.CH.2017.01.en10.2305/IUCN.CH.2017.01.en ↗
6. Dris R, Gasperi J, Mirande C, Mandin C, Guerrouache M, Langlois V, Tassin B (2017) This is the first overview of textile fibers, including microplastics, in indoor and outdoor environments. Environ Pollut 221:453–458. https://doi.org/10.1016/j.envpol.2016.12.01310.1016/j.envpol.2016.12.013 ↗
7. Qu H, Diao H, Han J, Wang B, Yu G (2023) Understanding and addressing the environmental risk of microplastics. Frontiers of Environmental Science and Engineering 17(1):12. https://doi.org/10.1007/s11783-023-1612-510.1007/s11783-023-1612-5 ↗
8. Lim X (2021) Microplastics are everywhere, but are they harmful? Nature 593(7857):22–25. https://doi.org/10.1038/d41586-021-01143-310.1038/d41586-021-01143-3 ↗
9. De Sá LC, Oliveira M, Ribeiro F, Rocha TL, Futter MN (2018) Studies of the effects of microplastics on aquatic organisms: what do we know and where should we focus our efforts in the future? Sci Total Environ 645:1029–1039. https://doi.org/10.1016/j.scitotenv.2018.07.20710.1016/j.scitotenv.2018.07.207 ↗
10. Senathirajah K, Attwood S, Bhagwat G, Carbery M, Wilson S, Palanasami T (2021) Estimation of the mass of microplastics ingested—a pivotal first step towards human health assessment. J Hazard Mater 404:12404. https://doi.org/10.1016/j.jhazmat.2020.12400410.1016/j.jhazmat.2020.124004 ↗
11. Barboza LGA, Dick Vethaak A, Lavorante BRBO, Lundebicycle AK, Guilhermino L (2018) Marine microplastic debris: an emerging issue for food security, food safety, and human health. Mar Pollut Bull 133:336–348. https://doi.org/10.1016/j.marpolbul.2018.05.04710.1016/j.marpolbul.2018.05.047 ↗
12. Ragusa A, Svelato A, Santacroce C, Catalano P, Notarstefano V, Carnevali O, Papa F, Rongioletti MC, Baiocco F, Draghi S, D’Amore E, Rinaldo D, Matta M, Giorgini E (2021) Plasticenta: first evidence of microplastics in human placenta. Environ Int 146:106274. https://doi.org/10.1016/j.envint.2020.10627410.1016/j.envint.2020.106274 ↗
13. Maher BA, Ahmed IAM, Karloukovski V, MacLaren DA, Foulds PG, Allsop D, Mann DMA, Torres-Jardón R, Calderon-Garciduenas L (2016) Magnetite pollution nanoparticles in the human brain. Proc Natl Acad Sci USA 113(39):10797–10801. https://doi.org/10.1073/pnas.160594111310.1073/pnas.1605941113 ↗
14. Jeong CB, Won EJ, Kang HM, Lee MC, Hwang DS, Hwang UK, Zhou B, Souissi S, Lee SJ, Lee JS (2016) Microplastic size-dependent toxicity, oxidative stress induction, and p-JNK and p-p38 activation in the monogonont rotifer (Brachionus koreanus). Environ Sci Technol 50(16):8849–8857. https://doi.org/10.1021/acs.est.6b0144110.1021/acs.est.6b01441 ↗
15. Wang J, Zhao X, Wu F, Niu L, Tang Z, Liang W, Zhao T, Fang M, Wang H, Wang X (2021) Characterization, occurrence, environmental behaviors, and risks of nanoplastics in the aquatic environment: current status and future perspectives. Fundam Res 1(3):317–328. https://doi.org/10.1016/j.fmre.2021.05.00110.1016/j.fmre.2021.05.001 ↗
16. Collard F, Gilbert B, Eppe G, Parmentier E, Das K (2015) Detection of anthropogenic particles in fish stomachs: an isolation method adapted for identification by Raman spectroscopy. Arch Environ Contam Toxicol 69(3):331–339. https://doi.org/10.1007/s00244-015-0221-010.1007/s00244-015-0221-0 ↗
17. Deng Y, Zhang Y, Lemos B, Ren H (2017) Tissue accumulation of microplastics in mice and biomarker responses suggest widespread health risks from exposure to microplastics. Sci Rep 7:46687. https://doi.org/10.1038/srep4668710.1038/srep46687 ↗
18. Jenner LC, Rotchell JM, Bennett RT, Cowen M, Tentzeris V, Sadofsky LR (2022) Detection of microplastics in human lung tissue using μFTIR spectroscopy. Sci Total Environ 831:154907. https://doi.org/10.1016/j.scitotenv.2022.15490710.1016/j.scitotenv.2022.154907 ↗
19. Horvatits T, Tamminga M, Liu B, Sebode M, Carambia A, Fischer L, Püschel K, Huber S, Fischer EK (2022) Microplastics detected in cirrhotic liver tissue. EBioMedicine 82:104147. https://doi.org/10.1016/j.ebiom.2022.10414710.1016/j.ebiom.2022.104147 ↗
20. Smith AM, Mancini MC, Nie S (2009) Bioimaging: second window for in vivo imaging. Nat Nanotechnol 4(11):710–711. https://doi.org/10.1038/nnano.2009.32610.1038/nnano.2009.326 ↗
21. Soga K, Umezawa M, Okubo K (2021) Transparency in Biology. Springer, Singapore10.1007/978-981-15-9627-8 ↗
22. Ichihashi K, Umezawa M, Ueya Y, Okubo K, Takamoto E, Matsuda T, Soga K (2022) Effect of the enantiomeric structure of hydrophobic polymers on the encapsulation properties of a second near infrared (NIR-II) fluorescent dye for in vivo deep imaging. RSC advances 12(3):1310–1318. https://doi.org/10.1039/d1ra08330a10.1039/d1ra08330a ↗
23. Mi J, Li C, Yang F (2024) Comparative study of indocyanine green fluorescence imaging in lung cancer with near-infrared-I/II windows. Ann Surg Oncol 31:2451–2460. https://doi.org/10.1245/s10434-023-14677-910.1245/s10434-023-14677-9 ↗
24. Li C, Mi J, Wang Y (2023) New and effective EGFR-targeted fluorescence imaging technology for intraoperative rapid determination of lung cancer in freshly isolated tissue. Eur J Nucl Med Mol Imaging 50, 494–507. https://doi.org/10.1007/s00259-022-05975-710.1007/s00259-022-05975-7 ↗
25. Umezawa M, Sera T, Yokota H, Takematsu M, Morita M, Yeroslavsky G, Kamimura M, Soga K (2020) Computed tomography for in vivo deep over-1000 nm near-infrared fluorescence imaging. J Biophotonics 13(8). https://doi.org/10.1002/jbio.20200007110.1002/jbio.202000071 ↗
26. Umezawa M, Miyata K, Okubo K, Soga K (2022) Three dimensional lifetime-multiplex tomography based on time-gated capturing of near-infrared fluorescence images. Appl Sci 12(15):7721. https://doi.org/10.3390/app1215772110.3390/app12157721 ↗
27. Li D, Liu K, Li C, Peng G, Andrady AL, Wu T, Zhang Z, Wang X, Song Z, Zong C, Zhang F, Wei N, Bai M, Zhu L, Xu J, Wu H, Wang L, Chang S, Zhu W (2020) Profiling the vertical transport of microplastics in the West Pacific Ocean and the East Indian Ocean using a novel in situ filtration technique. Environ Sci Technol 54(20):12979–12988. https://doi.org/10.1021/acs.est.0c0237410.1021/acs.est.0c02374 ↗
28. Umezawa M, Ueya Y, Ichihashi K, Dung DTK, Soga K (2023) Controlling molecular dye encapsulation in the hydrophobic core of core–shell nanoparticles for in vivo imaging. Biomedical Materials and Devices 1:605–617. https://doi.org/10.1007/s44174-023-00073-010.1007/s44174-023-00073-0 ↗
29. Brown DM, Wilson MR, MacNee W, Stone V, Donaldson K (2001) Size-dependent proinflammatory effects of ultrafine polystyrene particles: a role for surface area and oxidative stress in the enhanced activity of ultrafines. Toxicol Appl Pharmacol 175(3):191–199. https://doi.org/10.1006/taap.2001.924010.1006/taap.2001.9240 ↗
30. Inoue K, Takano H, Yanagisawa R, Koike E, Shimada A (2009) Size effects of latex nanomaterials on lung inflammation in mice. Toxicol Appl Pharmacol 234(1):68–76. https://doi.org/10.1016/j.taap.2008.09.01210.1016/j.taap.2008.09.012 ↗
31. Huang JP, Hsieh PCH, Chen CY, Wang TY, Chen PC, Liu CC, Chen CC, Chen CP (2015) Nanoparticles can cross the mouse placenta and induce trophoblast apoptosis. Placenta 36(12):1433–1441. https://doi.org/10.1016/j.placenta.2015.10.00710.1016/j.placenta.2015.10.007 ↗
32. Noventa S, Boyles MSP, Seifert A, Belluco S, Jiménez AS, Johnston HJ, Tran L, Fernandes TF, Mughini-Gras L, Orsini M, Corami F, Castro K, Mutinelli F, Boldrin M, Puntes V, Sotoudeh M, Mascarello G, Tiozzo B, McLean P, Ronchi F, Booth AM, Koelmans AA, Losasso C (2021) Paradigms to assess the human health risks of nano- and microplastics. Microplastics Nanoplastics 1(1):9. https://doi.org/10.1186/s43591-021-00011-110.1186/s43591-021-00011-1 ↗
33. Umezawa M, Kobayashi H, Ichihashi K, Sekiyama S, Okubo K, Kamimura M, Soga K (2022) Heat treatment effects for controlling dye molecular states in hydrophobic core of over-1000nm near-infrared (NIR-II) fluorescent micellar nanoparticles. ACS Omega 7(7):5817–5824. https://doi.org/10.1021/acsomega.1c0577110.1021/acsomega.1c05771 ↗
34. Umezawa M, Haruki M, Yoshida M, Kamimura M, Soga K (2021) Effects of processing pH on emission intensity of over-1000 nm near-infrared fluorescence of dye-loaded polymer micelle with polystyrene core. Anal Sci 37:485–489. https://doi.org/10.2116/analsci.20scp0910.2116/analsci.20scp09 ↗
35. Venkatachalam N, Saito Y, Soga K (2009) Synthesis of Er3+ doped Y2O3 nanophosphors. J Am Ceram Soc 92(5):1006–1010. https://doi.org/10.1111/j.1551-2916.2009.02986.x10.1111/j.1551-2916.2009.02986.x ↗
36. Okubo K, Takeda R, Murayama S, Umezawa M, Kamimura M, Osada K, Aoki I, Soga K (2021) Size-controlled bimodal in vivo nanoprobes as near-infrared phosphors and positive contrast agents for magnetic resonance imaging. Sci Technol Adv Mater 22:160–172. https://doi.org/10.1080/14686996.2021.188771210.1080/14686996.2021.1887712 ↗
37. Ueya Y, Umezawa M, Kobayashi Y, Ichihashi K, Kobayashi H, Matsuda T, Takamoto E, Kamimura M, Soga K (2022) Effects of hydrophilic/hydrophobic blocks ratio of PEG-b-PLGA on emission intensity and stability of over-1000 nm near-infrared (NIR-II) fluorescence dye-loaded polymeric micellar nanoparticles. Anal Sci 38(1):199–205. https://doi.org/10.2116/analsci.21P28310.2116/analsci.21P283 ↗
38. Song Y et al (2025) Microplastics in stools and their influencing factors among young adults from three cities in China: a multicenter cross-sectional study. Environ Pollut 364(Pt 2):12516810.1016/j.envpol.2024.125168 ↗