Targeting of sialoadhesin-expressing macrophages through antibody-conjugated (polyethylene glycol) poly(lactic-co-glycolic acid) nanoparticles
Source abstract
Abstract
This research aims to evaluate different-sized nanoparticles consisting of (polyethylene glycol) (PEG) poly(lactic-co-glycolic acid) (PLGA), loaded with fluorescein isothiocyanate for nanoparticle uptake and intracellular fate in sialoadhesin-expressing macrophages, while being functionalized with anti-sialoadhesin antibody. Sialoadhesin is a macrophage-restricted receptor, expressed on certain populations of resident tissue macrophages, yet is also upregulated in some inflammatory conditions. The nanocarriers were characterized for nanoparticle size (84-319 nm), zeta potential, encapsulation efficiency, and in vitro dye release. Small (86 nm) antibody-functionalized PEG PLGA nanoparticles showed persisting benefit from sialoadhesin-targeting after 24 h compared to the control groups. For small (105 nm) PLGA nanoparticles, uptake rate was higher for antibody-conjugated nanoparticles, though the total amount of uptake was not enhanced after 24 h. For both plain and functionalized small-sized (PEG) PLGA nanoparticles, no co-localization between nanoparticles and (early/late) endosomes nor lysosomes could be observed after 1-, 4-, or 24-h incubation time. In conclusion, decorating (PEG) PLGA nanocarriers with anti-sialoadhesin antibodies positively impacts macrophage targeting, though it was found to be formulation-specific.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Glycosylation and Glycoproteins Research · Nanoparticle-Based Drug Delivery · Advanced Drug Delivery Systems
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (41)
Show source-provided references
1. Asano K, Kikuchi K, Tanaka M (2018) CD169 macrophages regulate immune responses toward particulate materials in the circulating fluid. J Biochem 164(2):77–8510.1093/jb/mvy050 ↗
2. Chen W, Hu S (2011) Suitable carriers for encapsulation and distribution of endostar: comparison of endostar-loaded particulate carriers. Int J Nanomedicine 6(6):1535–1541
3. Chen WC, Kawasaki N, Nycholat CM et al (2012) Antigen delivery to macrophages using liposomal nanoparticles targeting Sialoadhesin/CD169. PLoS ONE 7(6):1–910.1371/journal.pone.0039039 ↗
4. Cheng FY, Wang SPH, Su CH et al (2008) Stabilizer-free poly(lactide-co-glycolide) nanoparticles for multimodal biomedical probes. Biomaterials 29(13):2104–211210.1016/j.biomaterials.2008.01.010 ↗
5. Delputte PL, van Gorp H, Favoreel HW et al (2011) Porcine sialoadhesin (CD169/Siglec-1) is an endocytic receptor that allows targeted delivery of toxins and antigens to macrophages. PLoS ONE 6(2):1–1210.1371/journal.pone.0016827 ↗
6. De Schryver M, Cappoen D, Elewaut D et al (2017b) Monoclonal antibody binding to the macrophage-specific receptor sialoadhesin alters the phagocytic properties of human and mouse macrophages. Cell Immunol 312:51–6010.1016/j.cellimm.2016.11.009 ↗
7. De Schryver M, Leemans A, Pintelon I et al (2017a) Comparative analysis of the internalization of the macrophage receptor sialoadhesin in human and mouse primary macrophages and cell lines. Immunobiology 222(6):797–80610.1016/j.imbio.2016.11.013 ↗
8. De Schryver M, Van Gorp H, Hoebeke I et al (2016) Development and characterization of new species cross-reactive anti-sialoadhesin monoclonal antibodies. Antibodies 5(7):1–18
9. Durán V, Yasar H, Becker J et al (2019) Preferential uptake of chitosan-coated PLGA nanoparticles by primary human antigen presenting cells. Nanomedicine Nanotechnol Biol Med 21:10207310.1016/j.nano.2019.102073 ↗
10. Elmowafy EM, Tiboni M, Soliman ME (2019) Biocompatibility, biodegradation and biomedical applications of poly (lactic acid)/ poly (lactic-co-glycolic acid) micro and nanoparticles. J Pharm Investig 49(4):347–38010.1007/s40005-019-00439-x ↗
11. Eshaghi B, Alsharif N, An X et al (2020) Stiffness of HIV-1 Mimicking polymer nanoparticles modulates ganglioside-mediated cellular uptake and trafficking. Adv Sci 7(18):1–1310.1002/advs.202000649 ↗
12. Gaspar N, Zambito G, Mezzanotte L (2019) Active nano-targeting of macrophages. Curr Pharm Des 25:1951–196110.2174/1381612825666190710114108 ↗
13. Gomes AJ, Faustino AS, Machado AEH et al (2006) Characterization of PLGA microparticles as a drug carrier for 3-ethoxycarbonyl-2H-benzofuro[3,2-f]-1-benzopyran-2-one. Ultrastructural study of cellular uptake and intracellular distribution. Drug Deliv 13(6):447–45410.1080/10717540600640369 ↗
14. Hartnell A, Steel J, Turley H, Jones M, Jackson DG, Crocker PR (2001) Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations. Blood 97(1):288–29610.1182/blood.V97.1.288 ↗
15. Hu J, Wei P, Seeberger PH, Yin J (2018) Mannose-functionalized nanoscaffolds for targeted delivery in biomedical applications. Chem - an Asian J 13(22):3448–345910.1002/asia.201801088 ↗
16. Jain NK, Mishra V, Mehra NK (2013) Targeted drug delivery to macrophages. Expert Opin Drug Deliv 10(3):353–36710.1517/17425247.2013.751370 ↗
17. Jones C, Virji M, Crocker PR (2003) Recognition of sialylated meningococcal lipopolysaccharide by siglecs expressed on myeloid cells leads to enhanced bacterial uptake. Mol Microbiol 49(5):1213–122510.1046/j.1365-2958.2003.03634.x ↗
18. Kalluru R, Fenaroli F, Westmoreland D et al (2013) Poly(lactide-co-glycolide)-rifampicin nanoparticles efficiently clear mycobacterium bovis BCG infection in macrophages and remain membrane-bound in phago-lysosomes. J Cell Sci 126(14):3043–3054
19. Kelly C, Jefferies C, Cryan S-A (2011) Targeted liposomal drug delivery to monocytes and macrophages. J Drug Deliv 2011:72724110.1155/2011/727241 ↗
20. Klaas M, Crocker PR (2012) Sialoadhesin in recognition of self and non-self. Semin Immunopathol 34(3):353–36410.1007/s00281-012-0310-3 ↗
21. Lee SY, Fierro JJ, Tran AM, Hong D, Espinal J, Dou H (2021) Cytoplasmic trafficking of nanoparticles delivers plasmid DNA for macrophage gene-editing. Curr Gene Ther 21:1–1210.2174/1566523221666210211101740 ↗
22. Li Z, Xiong F, He J, Dai X, Wang G (2016) Surface-functionalized, pH-responsive poly(lactic-co-glycolic acid)-based microparticles for intranasal vaccine delivery: effect of surface modification with chitosan and mannan. Eur J Pharm Biopharm 109:24–3410.1016/j.ejpb.2016.08.012 ↗
23. Nijen Twilhaar MK, Czentner L, Grabowska J et al (2020) Optimization of liposomes for antigen targeting to splenic cd169+ macrophages. Pharmaceutics 12(12):1–2110.3390/pharmaceutics12121138 ↗
24. O’Neill ASG, Van Den Berg TK, Mullen GED (2013) Sialoadhesin - a macrophage-restricted marker of immunoregulation and inflammation. Immunology 138(3):198–20710.1111/imm.12042 ↗
25. Ohnishi K, Komohara Y, Saito Y et al (2013) CD169-positive macrophages in regional lymph nodes are associated with a favorable prognosis in patients with colorectal carcinoma. Cancer Sci 104(9):1237–124410.1111/cas.12212 ↗
26. Ooms K, Van Gorp H, Van Gaever T, Nauwynck HJ, Delputte PL (2013a) Development of a recombinant antibody to target peptides and proteins to sialoadhesin-expressing macrophages. BMC Biotechnol 13(33):1–10
27. Ooms K, Van Gorp H, Botti S, Van Gaever T, Delputte PL, Nauwynck HJ (2013b) Evaluation of viral peptide targeting to porcine sialoadhesin using a porcine reproductive and respiratory syndrome virus vaccination-challenge model. Virus Res 177(2):147–15510.1016/j.virusres.2013.07.019 ↗
28. Pang L, Zhu Y, Qin J, Zhao W, Wang J (2018) Primary M1 macrophages as multifunctional carrier combined with PLGA nanoparticle delivering anticancer drug for efficient glioma therapy nanoparticle delivering anticancer drug for efficient glioma therapy. Drug Deliv 25(1):1922–193110.1080/10717544.2018.1502839 ↗
29. Patra JK, Das G, Fraceto LF et al (2018) Nano based drug delivery systems : recent developments and future prospects. J Nanobiotechnology 16(71):1–33
30. Pei Y, Yeo Y (2016) Drug delivery to macrophages: challenges and opportunities. J Control Release 240:202–21110.1016/j.jconrel.2015.12.014 ↗
31. Qi F, Wu J, Li H, Ma G (2019) Recent research and development of PLGA/PLA microspheres/nanoparticles: a review in scientific and industrial aspects. Front Chem Sci Eng 13(1):14–2710.1007/s11705-018-1729-4 ↗
32. Schliehe C, Schliehe C, Thiry M et al (2011) Microencapsulation of inorganic nanocrystals into PLGA microsphere vaccines enables their intracellular localization in dendritic cells by electron and fluorescence microscopy. J Control Release 151(3):278–28510.1016/j.jconrel.2011.01.005 ↗
33. Suk JS, Xu Q, Kim N, Hanes J, Ensign LM (2016) PEGylation as a strategy for improving nanoparticle-based drug and gene delivery Jung. Adv Drug Deliv Rev 99:28–5110.1016/j.addr.2015.09.012 ↗
34. Trombone APF, Silva CL, Almeida LP et al (2007) Tissue distribution of DNA-Hsp65/TDM-loaded PLGA microspheres and uptake by phagocytic cells. Genet Vaccines Ther 5:1–810.1186/1479-0556-5-9 ↗
35. Vandervoort J, Ludwig A (2002) Biocompatible stabilizers in the preparation of PLGA nanoparticles: a factorial design study. Int J Pharm 238:77–9210.1016/S0378-5173(02)00058-3 ↗
36. Van Hees S, Elbrink K, De Schryver M, Delputte PL, Kiekens F (2020) Improving cellular uptake and cytotoxicity of chitosan-coated poly(lactic-co-glycolic acid) nanoparticles in macrophages. Nanomedicine 15(27):2671–268810.2217/nnm-2020-0317 ↗
37. Vu-Quang H, Vinding MS, Xia D et al (2016) Chitosan-coated poly(lactic-co-glycolic acid) perfluorooctyl bromide nanoparticles for cell labeling in 19F magnetic resonance imaging. Carbohydr Polym 136:936–94410.1016/j.carbpol.2015.09.076 ↗
38. Wagh A, Law B (2013) Chapter 15 methods for conjugating antibodies to nanocarriers. In: Ducry L (ed) Antibody-drug conjugates, methods in molecular biology, vol 1045. Springer Science+Business Media, LLC. https://doi.org/10.1007/978-1-62703-541-5_1510.1007/978-1-62703-541-5_15 ↗
39. Yameen B, Choi WI, Vilos C, Swami A, Shi J, Farokhzad OC (2014) Insight into nanoparticle cellular uptake and intracellular targeting. J Control Release 190:485–49910.1016/j.jconrel.2014.06.038 ↗
40. Yang H, Lj K, Liu Y, Liu Z, Miyoshi H (2009) Poly(D, L-lactide-co-glycolide) nanoparticles encapsulated fluorescent isothiocyanate and paclitaxol: preparation, release kinetics and anticancer effect. J Nanosci Nanotechnol 9(1):282–28710.1166/jnn.2009.J065 ↗
41. Zhang J, Chang D, Yang Y et al (2017) Systematic investigation on the intracellular trafficking network of polymeric nanoparticles. Nanoscale 9(9):3269–328210.1039/C7NR00532F ↗