Protective effect of hesperidin-loaded selenium nanoparticles stabilized by chitosan on glutamate-induced toxicity in PC12 cells
Source abstract
Abstract
Hesperidin as a natural flavonoid has a wide range of beneficial properties such as anti-oxidant, anti-cancer, and anti-inflammatory effects. However, low water solubility and low bioavailability have limited its use in clinic. Selenium nanoparticles have attracted great interest in recent years due to their unique characteristics and anti-oxidant activates. Therefore, in this study, we synthesized hesperidin-loaded selenium nanoparticles stabilized by chitosan (Hesp-SeNPs@CS) and investigated its protective effects against glutamate-induced toxicity in PC12 cells. The physicochemical properties of the nanoparticles were evaluated using various techniques. The MTT and intracellular reactive oxygen species (ROS) assay were performed to evaluate the cell viability and ROS levels, respectively. In addition, flow cytometry was used to determine the cell cycle arrest and apoptosis rate in PC12 cells. Our results showed that, Hesp-SeNPs@CS nanoparticles have a 210.6 nm diameter and a negative zeta potential of -15.9 mV. The results of MTT assay revealed that glutamate significantly decreased cell viability by about 50% at a concentration of 20 mM. However, hesperidin at the concentration of 62.5 µM and Hesp-SeNPs@CS at the concentrations of 62.5 and 125 μg/ml significantly decreased the glutamate-induced cell toxicity, sub-G1 cell cycle arrest and apoptosis rate in PC12 cells. In addition, in the groups treated with hesperidin and Hesp-SeNPs@CS, the intracellular ROS induced by glutamate showed a significant decrease compared to glutamate-treated group. Taken together, these results revealed that Hesp-SeNPs@CS has protective effects against glutamate-induced cytotoxicity and oxidative stress damage in PC12 cells that merit further investigation. Graphical abstract
Source: expertdata · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Selenium in Biological Systems · Curcumin's Biomedical Applications · Free Radicals and Antioxidants
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
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