In situ electron microscopy techniques for nanoparticle dispersion analysis of commercial sunscreen
Source abstract
Abstract
Abstract Nanoparticles are common active ingredients within many commercial products including sunscreen. Consequently, accurate characterisation of nanoparticles in these products is vital to enhance product design, whilst also understanding the toxicological implications of these nanoparticles. Whilst bulk techniques are useful in providing some information, they often cannot resolve individual particles, and therefore electron microscopy can be used for high-resolution nanoparticle characterisation. However, conventional high vacuum dry TEM does not accurately represent nanoparticle dispersions and other in situ methods must be used. Here, we use a combination of techniques including liquid cell transmission electron microscopy (LCTEM), cryogenic (cryo)-TEM and cryo-scanning electron microscopy (SEM) to characterise a commercial sunscreen containing titanium dioxide (TiO2) and zinc oxide (ZnO) nanoparticles. Our work illustrates that whilst LCTEM does not require any sample preparation more beam artefacts can occur causing ZnO dissolution with only TiO2 nanoparticles visualised. Comparatively, cryo-TEM allows characterisation of both ZnO and TiO2, yet only cryo-SEM could be used to analyse the pure product (without dilution) but biased the characterisation to the larger fraction of nanoparticles and agglomerates. Ultimately, only with a combination of different in situ EM techniques can an accurate characterisation of commercial products be achieved in order to ensure effective and safe product design and manufacture.
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Research context
OpenAlex topics: Nanoparticles: synthesis and applications · Gold and Silver Nanoparticles Synthesis and Applications · TiO2 Photocatalysis and Solar Cells
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (31)
Show source-provided references
1. Cole C, Shyr T, Ou-Yang H (2016) Metal oxide sunscreens protect skin by absorption, not by reflection or scattering. Photodermatol Photoimmunol Photomed 32(1):5–1010.1111/phpp.12214 ↗
2. Popov AP et al (2005) TiO2nanoparticles as an effective UV-B radiation skin-protective compound in sunscreens. J Phys D Appl Phys 38(15):2564–257010.1088/0022-3727/38/15/006 ↗
3. Tyner KM et al (2011) The state of nano-sized titanium dioxide (TiO2) may affect sunscreen performance. Int J Cosmet Sci 33(3):234–24410.1111/j.1468-2494.2010.00622.x ↗
4. Barnard AS (2010) One-to-one comparison of sunscreen efficacy, aesthetics and potential nanotoxicity. Nat Nanotechnol 5(4):271–27410.1038/nnano.2010.25 ↗
5. Lewicka ZA et al (2011) The structure, composition, and dimensions of TiO2 and ZnO nanomaterials in commercial sunscreens. J Nanopart Res 13(9):360710.1007/s11051-011-0438-4 ↗
6. Braydich-Stolle LK et al (2009) Crystal structure mediates mode of cell death in TiO2 nanotoxicity. J Nanopart Res 11(6):1361–137410.1007/s11051-008-9523-8 ↗
7. Davey S (2010) Sunscreen size studies. Nat Chem10.1038/nchem.625 ↗
8. Ilett M, Wills J, Rees P, Sharma S, Micklethwaite S, Brown A, Brydson R, Hondow N (2020) Application of automated electron microscopy imaging and machine learning to characterise and quantify nanoparticle dispersion in aqueous media. J Microsc 279:177–184. https://doi.org/10.1111/jmi.1285310.1111/jmi.12853 ↗
9. Ispas C et al (2009) Toxicity and developmental defects of different sizes and shape nickel nanoparticles in zebrafish. Environ Sci Technol 43(16):6349–635610.1021/es9010543 ↗
10. Favi PM et al (2015) Shape and surface effects on the cytotoxicity of nanoparticles: Gold nanospheres versus gold nanostars. J Biomed Mater Res A 103(11):3449–346210.1002/jbm.a.35491 ↗
11. Deegan RD et al (1997) Capillary flow as the cause of ring stains from dried liquid drops. Nature 389(6653):827–82910.1038/39827 ↗
12. Dieckmann Y et al (2009) Particle size distribution measurements of manganese-doped ZnS nanoparticles. Anal Chem 81(10):3889–389510.1021/ac900043y ↗
13. Ilett M et al (2019) Cryo-analytical STEM of frozen, aqueous dispersions of nanoparticles. Micron 120:35–4210.1016/j.micron.2019.01.013 ↗
14. Woehl TJ et al (2013) Experimental procedures to mitigate electron beam induced artifacts during in situ fluid imaging of nanomaterials. Ultramicroscopy 127:53–6310.1016/j.ultramic.2012.07.018 ↗
15. Schneider NM et al (2014) Electron–water interactions and implications for liquid cell electron microscopy. J Phys Chem C 118(38):22373–2238210.1021/jp507400n ↗
16. Elliot AJ, Bartels DM (2009) The reaction set, rate constants and g-values for the simulation of the radiolysis of light water over the range 20 deg to 350 deg C based on information available in 2008. Atomic Energy of Canada Ltd; Report number AECL-153–127160–450–001: Canada, p 162
17. Sterniczuk M, Bartels DM (2016) Source of molecular hydrogen in high-temperature water radiolysis. J Phys Chem A 120(2):200–20910.1021/acs.jpca.5b12281 ↗
18. Speedy RJ, Angell CA (1976) Isothermal compressibility of supercooled water and evidence for a thermodynamic singularity at −45°C. J Chem Phys 65(3):851–85810.1063/1.433153 ↗
19. Ilett M, Freeman HM, Aslam Z, Galloway JM, Klebl DP, Muench SP, McPherson IJ, Cespedes O, Kim Y-Y, Meldrum FC, Yeandel SR, Freeman CL, Harding JH, Brydson RMD (2022) Evaluation of correlated studies using liquid cell and cryo-transmission electron microscopy: hydration of calcium sulphate and the phase transformation pathways of bassanite to gypsum. J Microsc 288:155–168. https://doi.org/10.1111/jmi.1310210.1111/jmi.13102 ↗
20. Liu K-L et al (2008) Novel microchip for in situTEM imaging of living organisms and bio-reactions in aqueous conditions. Lab Chip 8(11):1915–192110.1039/b804986f ↗
21. Lu PJ et al (2015) Characterizing titanium dioxide and zinc oxide nanoparticles in sunscreen spray. Int J Cosmet Sci 37(6):620–62610.1111/ics.12239 ↗
22. Trang NTT, Chang J, Chen W-A, Chen C-C, Chen, H-M, Chang C-C, Fong T-H (2020) A novel microchip technique for quickly identifying nanogranules in an aqueous solution by transmission electron microscopy: imaging of platelet granules. Appl Sci 10:4946. https://doi.org/10.3390/app1014494610.3390/app10144946 ↗
23. Tai L-A et al (2012) Quantitative characterization of nanoparticles in blood by transmission electron microscopy with a window-type microchip nanopipet. Anal Chem 84(15):6312–631610.1021/ac301523n ↗
24. BioMa-Tek. MA-tek's enabling solution for liquid analysis by TEM; https://www.bioma-tek.com/bioma-tek/en/goods.php?act=view&no=61. [cited 2023 Jan]
25. Le Caër S (2011) Water radiolysis: influence of oxide surfaces on H2 production under ionizing radiation. Water 3(1):23510.3390/w3010235 ↗
26. Bian S-W et al (2011) Aggregation and dissolution of 4 nm ZnO nanoparticles in aqueous environments: influence of pH, ionic strength, size, and adsorption of humic acid. Langmuir 27(10):6059–606810.1021/la200570n ↗
27. Mu Q et al (2014) Systematic investigation of the physicochemical factors that contribute to the toxicity of ZnO nanoparticles. Chem Res Toxicol 27(4):558–56710.1021/tx4004243 ↗
28. Woehl TJ, Abellan P (2017) Defining the radiation chemistry during liquid cell electron microscopy to enable visualization of nanomaterial growth and degradation dynamics. J Microsc 265(2):135–14710.1111/jmi.12508 ↗
29. Ruszkiewicz JA et al (2017) Neurotoxic effect of active ingredients in sunscreen products, a contemporary review. Toxicol Rep 4:245–25910.1016/j.toxrep.2017.05.006 ↗
30. Soenen SJH et al (2010) Intracellular nanoparticle coating stability determines nanoparticle diagnostics efficacy and cell functionality. Small 6(19):2136–214510.1002/smll.201000763 ↗
31. Liu J et al (2017) Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner. Int J Nanomed 12:8085–809910.2147/IJN.S149070 ↗