Facile method for CLSM imaging unfunctionalized Au nanoparticles through fluorescent channels
Source abstract
Abstract
The microscopic visualization of metal nanoparticles has become a useful tool for the investigation of their applications in cell labeling and the study of their bio-effects. In the current study, we have developed a facile method with confocal laser scanning microscope (CLSM) to observe unfunctionalized Au nanoparticles through fluorescent channels. The sharp reflected signal and photostable property of the metal nanoparticles makes the present method very ideal for fluorescent co-localization, real-time imaging, and further quantitative analysis.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Gold and Silver Nanoparticles Synthesis and Applications · Photoacoustic and Ultrasonic Imaging · Protein Interaction Studies and Fluorescence Analysis
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (23)
Show source-provided references
1. Alschinger M, Maniak M, Stietz F, Vartanyan T, Trager F (2003) Application of metal nanoparticles in confocal laser scanning microscopy: improved resolution by optical field enhancement. Appl Phys B 76:771–774. doi: 10.1007/s00340-003-1182-y10.1007/s00340-003-1182-y ↗
2. Bhattacharya R, Patra CR, Verma R, Kumar S, Greipp PR, Mukherjee P (2007) Gold nanoparticles inhibit the proliferation of multiple myeloma cells. Adv Mater 19:711–716. doi: 10.1002/adma.20060209810.1002/adma.200602098 ↗
3. De Wall SL, Painter C, Stone JD, Bandaranayake R, Wiley DC, Mitchison TJ, Stern LJ, Dedecker BS (2006) Noble metals strip peptides from class II MHC proteins. Nat Chem Biol 2:197–201. doi: 10.1038/nchembio77310.1038/nchembio773 ↗
4. Delie F (1998) Evaluation of nano- and microparticle uptake by the gastrointestinal tract. Adv Drug Deliv Rev 34:221–233. doi: 10.1016/S0169-409X(98)00041-610.1016/S0169-409X(98)00041-6 ↗
5. Frens G (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold suspension. Nat Phys Sci (Lond) 241:20–2210.1038/physci241020a0 ↗
6. Geanacopoulos M, Gear AR (1988) Application of spray-freezing to the study of rapid platelet reactions by a quenched-flow approach. Thromb Res 52:599–607. doi: 10.1016/0049-3848(88)90132-610.1016/0049-3848(88)90132-6 ↗
7. Haberle W, Horber JK, Ohnesorge F, Smith DP, Binnig G (1992) In situ investigations of single living cells infected by viruses. Ultramicroscopy 42–44:1161–1167. doi: 10.1016/0304-3991(92)90418-J10.1016/0304-3991(92)90418-J ↗
8. Hoffman A, Goetz M, Vieth M, Galle PR, Neurath MF, Kiesslich R (2006) Confocal laser endomicroscopy: technical status and current indications. Endoscopy 38:1275–1283. doi: 10.1055/s-2006-94481310.1055/s-2006-944813 ↗
9. Inoue K, Kurosumi K, Deng ZP (1982) An improvement of the device for rapid freezing by use of liquid propane and the application of immunocytochemistry to the resin section of rapid-frozen, substitution-fixed anterior pituitary gland. J Electron Microsc (Tokyo) 31:93–97
10. Itoh J, Sanno N, Matsuno A, Itoh Y, Watanabe K, Osamura RY (1997) Application of confocal laser scanning microscopy (CLSM) to visualize prolactin (PRL) and PRL mRNA in the normal and estrogen-treated rat pituitary glands using non-fluorescent Probes. Microsc Res Tech 39:157–167. doi: 10.1002/(SICI)1097-0029(19971015)39:2<157::AID-JEMT7>3.0.CO;2-I10.1002/(SICI)1097-0029(19971015)39:2<157::AID-JEMT7>3.0.CO;2-I ↗
11. Kienberger F, Stroh C, Kada G, Moser R, Baumgartner W, Pastushenko V, Rankl C, Schmidt U, Muller H, Orlova E, LeGrimellec C, Drenckhahn D, Blaas D, Hinterdorfer P (2003) Dynamic force microscopy imaging of native membranes. Ultramicroscopy 97:229–237. doi: 10.1016/S0304-3991(03)00047-010.1016/S0304-3991(03)00047-0 ↗
12. Li JY, Wang XM, Wang CX, Chen BA, Dai YY, Zhang RY, Song M, Lv G, Fu DG (2007) The enhancement effect of gold nanoparticles in drug delivery and as biomarkers of drug-resistant cancer cells. ChemMedChem 2:374–378. doi: 10.1002/cmdc.20060026410.1002/cmdc.200600264 ↗
13. Liu YP, Meyer-Zaika W, Franzka S, Schmid G, Tsoli M, Kuhn H (2003) Gold-cluster degradation by the transition of B-DNA into A-DNA and the formation of nanowires. Angew Chem Int Ed 42:2853–2857. doi: 10.1002/anie.20025023510.1002/anie.200250235 ↗
14. Murata F, Suzuki S, Tsuyama S, Suganuma T, Imada M, Furihata C (1985) Application of rapid freezing followed by freeze-substitution acrolein fixation for cytochemical studies of the rat stomach. Histochem J 17:967–980. doi: 10.1007/BF0141794610.1007/BF01417946 ↗
15. Pan Y, Neuss S, Leifert A, Fischler M, Wen F, Simon U, Schmid G, Brandau W, Jahnen-Dechent W (2007) Size-dependent cytotoxicity of gold nanoparticles. Small 3:1941–1949. doi: 10.1002/smll.20070037810.1002/smll.200700378 ↗
16. Patel DV, McGhee CN (2007) Contemporary in vivo confocal microscopy of the living human cornea using white light and laser scanning techniques: a major review. Clin Exp Ophthalmol 35:71–88. doi: 10.1111/j.1442-9071.2007.01423.x10.1111/j.1442-9071.2007.01423.x ↗
17. Prasad V, Semwogerere D, Weeks ER (2007) Confocal microscopy of colloids. J Phys Condens Matter 19:113102–113126. doi: 10.1088/0953-8984/19/11/11310210.1088/0953-8984/19/11/113102 ↗
18. Rhode S, Breuer A, Hesse J, Sonnleitner M, Pagler TA, Doringer M, Schutz GJ, Stangl H (2004) Visualization of the uptake of individual HDL particles in living cells via the scavenger receptor class B type I. Cell Biochem Biophys 41:343–356. doi: 10.1385/CBB:41:3:34310.1385/CBB:41:3:343 ↗
19. Shukla R, Bansal V, Chaudhary M, Basu A, Bhonde RR, Sastry M (2005) Biocompatibility of gold nanoparticles and their endocytotic fate inside the cellular compartment: a microscopic overview. Langmuir 21:10644–10654. doi: 10.1021/la051371210.1021/la0513712 ↗
20. Tkachenko AG, Xie H, Liu YL, Coleman D, Ryan J, Glomm WR, Shipton MK, Franzen S, Feldheim DL (2004) Cellular trajectories of peptide-modified gold particle complexes: comparison of nuclear localization signals and peptide transduction domains. Bioconjug Chem 15:482–490. doi: 10.1021/bc034189q10.1021/bc034189q ↗
21. Tseng CY, Ling EA, Wong WC (1984) A scanning and transmission electron microscopic study of amoeboid microglial cells in the prenatal rat brain following a maternal injection of 6-aminonicotinamide. J Anat 138:733–743
22. White JG, Amos WB, Fordham M (1987) An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy. J Cell Biol 105:41–48. doi: 10.1083/jcb.105.1.4110.1083/jcb.105.1.41 ↗
23. Yi H, Leunissen J, Shi G, Gutekunst C, Hersch S (2001) A novel procedure for pre-embedding double immunogold-silver labeling at the ultrastructural level. J Histochem Cytochem 49:279–28410.1177/002215540104900301 ↗