Copper(I) oxide nanoparticle and tryptophan as its biological conjugate: a modulation of cytotoxic effects
Source abstract
Abstract
Recent investigations indicated that copper oxide nanoparticles can selectively induce apoptosis and effectively suppress the proliferation of tumor cells. Thus, it showed a great potential to be used as a drug for cancer treatment. Here we report an easy synthesis of spheroidal cuprous oxide nanoparticles (CuNPs) and their organic conjugate with l-tryptophan (Trp) using surfactant, sodium dodecyl sulfate as a capping reagent. The particles looked golden yellow and showed a strong affinity to bind blood carrier proteins such as bovine serum albumin and human serum albumin. However, both optical behavior and texture of the particles altered upon conjugation with Trp. The average size of the CuNPs was estimated to be ~70 nm as appeared under transmission electron microscope or atomic force microscope. The biological conjugate with Trp was ~85 nm and looked light sky blue in aqueous suspension. The surface of the conjugated nanoparticles was smoother than the bare CuNPs. The CuNPs were found to be toxic to different cultured cancerous cells; however, conjugation with Trp attenuated the toxicity, and indicated its possible utility in developing a drug candidate for cancer in a controlled fashion. Reduced toxicity also indicated a possible use of the conjugated particle as a drug delivery system.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanoparticle-Based Drug Delivery · Gold and Silver Nanoparticles Synthesis and Applications · Copper-based nanomaterials and applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (50)
Show source-provided references
1. Adleman JR, Boyd DA, Goodwin DG, Psaltis D (2009) Heterogeneous catalysis mediated by plasmon heating. Nano Lett 9:4417–442310.1021/nl902711n ↗
2. Akhtar MJ, Ahamed M, Kumar S, Khan MAM, Ahmad J, Alrokayan SA (2012) Zinc oxide nanoparticles selectively induce apoptosis in human cancer cells through reactive oxygen species. Int J Nanomed 7:845–857
3. Austin LA, Kang B, Yen C-W, El-Sayed MA (2011) Plasmonic imaging of human oral cancer cell communities during programmed cell death by nuclear-targeting silver nanoparticles. J Am Chem Soc 133:17594–1759710.1021/ja207807t ↗
4. Barillet S, Simon-Deckers A, Herlin-Boime N, Mayne-L’Hermite M, Reynaud C, Cassio D, Gouget B, Carriere M (2010) Toxicological consequences of TiO2, SiC nanoparticles and multi-walled carbon nanotubes exposure in several mammalian cell types: an in vitro study. J Nanopart Res 12:61–7310.1007/s11051-009-9694-y ↗
5. Bian Q, Liu J, Tian J, Hu Z (2004) Binding of genistein to human serum albumin demonstrated using tryptophan fluorescence quenching. Int J Biol Macromol 34:333–33710.1016/j.ijbiomac.2004.09.005 ↗
6. Chen PC, Mwakwari SC, Oyelere AK (2008) Gold nanoparticles: from nanomedicine to nanosensing. Nanotechnol Sci Appl 1:45–6610.2147/NSA.S3707 ↗
7. Chen Y-S, Hung Y-C, Liau I, Huang GS (2009) Assessment of the in vivo toxicity of gold nanoparticles. Nanoscale Res Lett 4:858–86410.1007/s11671-009-9334-6 ↗
8. Chen T, Shukoor MI, Wang R, Zhao Z, Yuan Q, Bamrungsap S, Xiong X, Tan W (2011) Smart multifunctional nanostructure for targeted cancer chemotherapy and magnetic resonance imaging. ACS Nano 5:7866–787310.1021/nn202073m ↗
9. Chevrier DM, Chatt A, Zhang P (2012) Properties and applications of protein-stabilized fluorescent gold nanoclusters: short review. J Nanophotonics 6:06450410.1117/1.JNP.6.064504 ↗
10. Choi HS, Liu W, Liu F, Nasr K, Misra P, Bawendi MG, Frangioni JV (2010) Design considerations for tumor-targeted nanoparticles. Nat Nanotechnol 5:42–4710.1038/nnano.2009.314 ↗
11. Eftink MR, Ghiron CA (1976) Fluorescence quenching of indole and model micelle systems. J Phys Chem 80:486–49310.1021/j100546a014 ↗
12. Fan C, Wang W, Zhao B, Zhang S, Miao J (2006) Chloroquine inhibits cell growth and induces cell death in A549 lung cancer cells. Bioorg Med Chem 14:3218–322210.1016/j.bmc.2005.12.035 ↗
13. Fan Z, Shelton M, Singh AK, Senapati D, Khan SA, Ray PC (2012) Multifunctional plasmonic shell-magnetic core nanoparticles for targeted diagnostics, isolation, and photothermal destruction of tumor cells. ACS Nano 6:1065–107310.1021/nn2045246 ↗
14. Feng L, Zhang C, Gao G, Cui D (2012) Facile synthesis of hollow Cu2O octahedral and spherical nanocrystals and their morphology-dependent photocatalytic properties. Nanoscale Res Lett 7:27610.1186/1556-276X-7-276 ↗
15. Gelperina S, Kisich K, Iseman MD, Heifets L (2005) The potential advantages of nanoparticle drug delivery systems in chemotherapy of tuberculosis. Am J Respir Crit Care Med 172:1487–149010.1164/rccm.200504-613PP ↗
16. Ghosh PS, Kim C-K, Han G, Forbes NS, Rotello VM (2008) Efficient gene delivery vectors by tuning the surface charge density of amino acid-functionalized gold nanoparticles. ACS Nano 2:2213–221810.1021/nn800507t ↗
17. Gou L, Murphy CJ (2003) Solution-phase synthesis of Cu2O nanocubes. Nano Lett 3:231–23410.1021/nl0258776 ↗
18. Hoffmann MR, Martin ST, Choi W, Bahnemann DW (1995) Environmental applications of semiconductor photocatalysis. Chem Rev 95:69–9610.1021/cr00033a004 ↗
19. Hussain SM, Hess KL, Gearhart JM, Geiss KT, Schlager JJ (2005) In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicol In Vitro 19:975–98310.1016/j.tiv.2005.06.034 ↗
20. Johnson BFG (2003) Nanoparticles in catalysis. Top Catal 24:147–15910.1023/B:TOCA.0000003086.83434.b6 ↗
21. Joshi P, Chakraborty S, Dey S, Shanker V, Ansari ZA, Singh SP, Chakrabarti P (2011) Binding of chloroquine-conjugated gold nanoparticles with bovine serum albumin. J Colloid Interface Sci 355:402–40910.1016/j.jcis.2010.12.032 ↗
22. Kamat PV (1993) Photochemistry on nonreactive and reactive (semiconductor) surfaces. Chem Rev 93:267–30010.1021/cr00017a013 ↗
23. Kuo C-H, Huang MH (2008) Facile synthesis of Cu2O nanocrystals with systematic shape evolution from cubic to octahedral structures. J Phys Chem C 112:18355–1836010.1021/jp8060027 ↗
24. Kuo C-H, Chen C-H, Huang MH (2007) Seed-mediated synthesis of monodispersed Cu2O nanocubes with five different size ranges from 40 to 420 nm. Adv Funct Mater 17:3773–378010.1002/adfm.200700356 ↗
25. Lakowicz JR (2006) Principles of fluorescence spectroscopy, 3rd edn. Springer, New York10.1007/978-0-387-46312-4 ↗
26. Lee SH, Bae KH, Kim SH, Lee KR, Park TG (2008) Amine-functionalized gold nanoparticles as non-cytotoxic and efficient intracellular siRNA delivery carriers. Int J Pharm 364:94–10110.1016/j.ijpharm.2008.07.027 ↗
27. Li F, Lei C, Shen Q, Li L, Wang M, Guo M, Huang Y, Nie Z, Yao S (2013) Analysis of copper nanoparticles toxicity based on a stress-responsive bacterial biosensor array. Nanoscale 5:653–66210.1039/C2NR32156D ↗
28. Livage J, Henry M, Sanchez C (1988) Sol–gel chemistry of transition metal oxides. Prog Solid State Chem 18:259–34110.1016/0079-6786(88)90005-2 ↗
29. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–6310.1016/0022-1759(83)90303-4 ↗
30. Nash MA, Waitumbi JN, Hoffman AS, Yager P, Stayton PS (2012) Multiplexed enrichment and detection of malarial biomarkers using a stimuli-responsive iron oxide and gold nanoparticle reagent system. ACS Nano 6:6776–678510.1021/nn3015008 ↗
31. Oskam G (2006) Metal oxide nanoparticles: synthesis, characterization and application. J Sol–Gel Sci Technol 37:161–16410.1007/s10971-005-6621-2 ↗
32. Park J, Park J-H, Ock K-S, Ganbold E-O, Song N-W, Cho K-C, Lee S-Y, Joo S-W (2011) Preferential adsorption of fetal bovine serum on bare and aromatic thiol-functionalized gold surfaces in cell culture media. J Colloid Interface Sci 363:105–11310.1016/j.jcis.2011.07.006 ↗
33. Pavia DL, Lampman GM, Kriz GS, Vyvyan JA (2009) Introduction to spectroscopy, 4th edn. Brooks/Cole Cengage Learning, Belmont
34. Pelicano H, Carew JS, McQueen TJ, Andreeff M, Plunkett W, Keating MJ, Huang P (2006) Targeting Hsp90 by 17-AAG in leukemia cells: mechanisms for synergistic and antagonistic drug combinations with arsenic trioxide and Ara-C. Leukemia 20:610–61910.1038/sj.leu.2404140 ↗
35. Prakash I, Muralidharan P, Nallamuthu N, Venkateswarlu M, Satyanarayana N (2007) Preparation and characterization of nanocrystallite size cuprous oxide. Mater Res Bull 42:1619–162410.1016/j.materresbull.2006.11.038 ↗
36. Premanathan M, Karthikeyan K, Jeyasubramanian K, Manivannan G (2011) Selective toxicity of ZnO nanoparticles toward Gram-positive bacteria and cancer cells by apoptosis through lipid peroxidation. Nanomedicine 7:184–19210.1016/j.nano.2010.10.001 ↗
37. Ramos AM, Aller P (2008) Quercetin decreases intracellular GSH content and potentiates the apoptotic action of the antileukemic drug arsenic trioxide in human leukemia cell lines. Biochem Pharmacol 75:1912–192310.1016/j.bcp.2008.02.007 ↗
38. Ravindra P (2009) Protein-mediated synthesis of gold nanoparticles. Mater Sci Eng B 163:93–9810.1016/j.mseb.2009.05.013 ↗
39. Rink JS, McMahon KM, Chen X, Mirkin CA, Thaxton CS, Kaufman DB (2010) Transfection of pancreatic islets using polyvalent DNA-functionalized gold nanoparticles. Surgery 148:335–34510.1016/j.surg.2010.05.013 ↗
40. Safi M, Yan M, Guedeau-Boudeville M-A, Conjeaud H, Garnier-Thibaud V, Boggetto N, Baeza-Squiban A, Niedergang F, Averbeck D, Berret J-F (2011) Interactions between magnetic nanowires and living cells: uptake, toxicity, and degradation. ACS Nano 5:5354–536410.1021/nn201121e ↗
41. Salata OV (2004) Applications of nanoparticles in biology and medicine. J Nanobiotechnol 2:1–610.1186/1477-3155-2-3 ↗
42. Salvati A, Pitek AS, Monopoli MP, Prapainop K, Bombelli FB, Hristov DR, Kelly PM, Aberg C, Mahon E, Dawson KA (2013) Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface. Nat Nanotechnol 8:137–14310.1038/nnano.2012.237 ↗
43. Torchilin VP (ed) (2006) Nanoparticulates as drug carriers. Imperial College Press, London
44. Wang Y, Zi X-Y, Su J, Zhang H-X, Zhang X-R, Zhu H-Y, Li J-X, Yin M, Yang F, Hu Y-P (2012) Cuprous oxide nanoparticles selectively induce apoptosis of tumor cells. Int J Nanomed 7:2641–2652
45. Wangoo N, Bhasin KK, Mehta SK, Suri CR (2008) Synthesis and capping of water-dispersed gold nanoparticles by an amino acid: bioconjugation and binding studies. J Colloid Interface Sci 323:247–25410.1016/j.jcis.2008.04.043 ↗
46. White AR, Multhaup G, Maher F, Bellingham S, Camakaris J, Zheng H, Bush AI, Beyreuther K, Masters CL, Cappai R (1999) The Alzheimer’s disease amyloid precursor protein modulates copper-induced toxicity and oxidative stress in primary neuronal cultures. J Neurosci 19:9170–917910.1523/JNEUROSCI.19-21-09170.1999 ↗
47. Wu Z, Zhang B, Yan B (2009) Regulation of enzyme activity through interactions with nanoparticles. Int J Mol Sci 10:4198–420910.3390/ijms10104198 ↗
48. Xu M, Li J, Iwai H, Mei Q, Fujita D, Su H, Chen H, Hanagata N (2012) Formation of nano-bio-complex as nanomaterials dispersed in a biological solution for understanding nanobiological interactions. Sci Rep 2:40610.1038/srep00406 ↗
49. Yang P-H, Sun X, Chiu J-F, Sun H, He Q-Y (2005) Transferrin-mediated gold nanoparticle cellular uptake. Bioconjugate Chem 16:494–49610.1021/bc049775d ↗
50. Yang K, Feng L, Shi X, Liu Z (2013) Nano-graphene in biomedicine: theranostic applications. Chem Soc Rev 42:530–54710.1039/C2CS35342C ↗