Physicochemical properties and in vitro cytotoxicity of iron oxide-based nanoparticles modified with antiangiogenic and antitumor peptide A7R
Source abstract
Abstract
Superparamagnetic iron oxide-based nanoparticles (SPIONs) are promising carriers as targeted drug delivery vehicles, because they can be guided to their target with the help of an external magnetic field. Functionalization of nanoparticles' surface with molecules, which bind with high affinity to receptors on target tissue significantly facilitates delivery of coated nanoparticles to their targeted site. Here, we demonstrate conjugation of an antiangiogenic and antitumor peptide ATWLPPR (A7R) to SPIONs modified with sebacic acid (SPIONs-SA). Successful conjugation was confirmed by various analytical techniques (FTIR, SERS, SEM-EDS, TEM, TGA). Cell cytotoxicity studies, against two cell lines (HUVEC and MDA-MB-231) indicated that SPIONs modified with A7R reduced HUVEC cell viability at concentrations higher than 0.01 mg Fe/mL, in comparison to cells that were exposed to either the nanoparticles modified with sebacic acid or A7R peptide solely, what might be partially caused by a process of internalization.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Nanoparticle-Based Drug Delivery · Advanced biosensing and bioanalysis techniques · RNA Interference and Gene Delivery
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (58)
Show source-provided references
1. Aliaga AE, Osorio-Román I, Leyton P, Garrido C, Cárcamo J, Caniulef C, Célis F, Diaz FG, Clavijo E, Gómez-Jeria JS, Campos-Vallette MM (2009) Surface-enhanced Raman scattering study of L-tryptophan. J Raman Spectrosc 40:164–169. doi: 10.1002/jrs.209910.1002/jrs.2099 ↗
2. Arruebo M, Fernández-Pacheco R, Ibarra MR, Santamaría J (2007) Magnetic nanoparticles for drug delivery. Nano Today 2:22–32. doi: 10.1016/S1748-0132(07)70084-110.1016/S1748-0132(07)70084-1 ↗
3. Bartczak D, Sanchez-Elsner T, Louafi F, Millar TM, Kanaras AG (2011) Receptor-mediated interactions between colloidal gold nanoparticles and human umbilical vein endothelial cells. Small 3:388–394. doi: 10.1002/smll.20100181610.1002/smll.201001816 ↗
4. Bartczak D, Muskens OL, Sanchez-Elsner T, Kanaras AG, Millar TM (2013) Manipulation of in vitro angiogenesis using peptide-coated gold nanoparticles. ACS Nano 7:5628–5636. doi: 10.1021/nn402111z10.1021/nn402111z ↗
5. Benachour H, Sève A, Bastogne T, Frochot C, Vanderesse R, Jasniewski J, Miladi I, Billotey C, Tillement O, Lux F, Barberi-Heyob M (2012) Multifunctional peptide-conjugated hybrid silica nanoparticles for photodynamic therapy and MRI. Theranostics 2:889–904. doi: 10.7150/thno.475410.7150/thno.4754 ↗
6. Bertrand N, Wu J, Xu X, Kamaly N, Farokhzad OC (2014) Cancer nanotechnology: the impact of passive and active targeting in the era of modern cancer biology. Adv Drug Deliv Rev 66:2–25. doi: 10.1016/j.addr.2013.11.00910.1016/j.addr.2013.11.009 ↗
7. Binétruy-Tournaire R, Demangel C, Malavaud B, Vassy R, Rouyre S, Kraemer M, Plouët J, Derbin C, Perret G, Mazie JC (2000) Identification of a peptide blocking vascular endothelial growth factor (VEGF)-mediated angiogenesis. EMBO J 19:1525–1533. doi: 10.1093/emboj/19.7.152510.1093/emboj/19.7.1525 ↗
8. Boohaker RJ, Lee MW, Vishnubhotla P, Perez JM, Khaled AR (2012) The use of therapeutic peptides to target and to kill cancer cells. Curr Med Chem 19:3794–3804. doi: 10.2174/09298671280166100410.2174/092986712801661004 ↗
9. Brzozowska M, Krysinski P (2009) Synthesis and functionalization of magnetic nanoparticles with covalently bound electroactive compound doxorubicin. Electrochim Acta 54:5065–5070. doi: 10.1016/j.electacta.2008.11.01810.1016/j.electacta.2008.11.018 ↗
10. Chaudhary B, Khaled YS, Ammori BJ, Elkord E (2014) Neuropilin 1: function and therapeutic potential in cancer. Cancer Immunol Immunother 63:81–99. doi: 10.1007/s00262-013-1500-010.1007/s00262-013-1500-0 ↗
11. Chuang C-H, Chen Y-T (2009) Raman scattering of L-tryptophan enhanced by surface plasmon of silver nanoparticles: vibrational assignment and structural determination. J Raman Spectrosc 40:150–156. doi: 10.1002/jrs.209710.1002/jrs.2097 ↗
12. Ciccione J, Jia T, Coll JL, Parra K, Amblard M, Jebors S, Martinez J, Mehdi A, Subra G (2016) Unambiguous and controlled one-pot synthesis of multifunctional silica nanoparticles. Chem Mater 28:885–889. doi: 10.1021/acs.chemmater.5b0439810.1021/acs.chemmater.5b04398 ↗
13. Da Silva SW, Nakagomi F, Silva MS, Franco A Jr, Garg VK, Oliveira AC, Morais PC (2010) Effect of the Zn content in the structural and magnetic properties of ZnxMg1-xFe2O4 mixed ferrites monitored by Raman and Mössbauer spectroscopies. J Appl Phys 107:09B503. doi: 10.1063/1.335090310.1063/1.3350903 ↗
14. De Palma R, Peeters S, Van Bael MJ, Van den Rul H, Bonroy K, Laureyn W, Mullens J, Borghs G, Maes G (2007) Silane ligand exchange to make hydrophobic superparamagnetic nanoparticles water-dispersible. Chem Mater 19:1821–1831. doi: 10.1021/cm062800010.1021/cm0628000 ↗
15. Dvorak HF (2002) Vascular permeability factor/vascular endothelial growth factor: a critical cytokine in tumor angiogenesis and a potential target for diagnosis and therapy. J Clin Oncol 20:4368–4380. doi: 10.1200/JCO.2002.10.08810.1200/JCO.2002.10.088 ↗
16. Ferrara N, Gerber HP, LeCouter J (2003) The biology of VEGF and its receptors. Nat Med 9:669–676. doi: 10.1038/nm0603-66910.1038/nm0603-669 ↗
17. Folkman J (1971) Tumor angiogenesis: therapeutic implications. N Engl J Med 285:1182–1186. doi: 10.1056/NEJM19711118285210810.1056/NEJM197111182852108 ↗
18. Fosgerau K, Hoffmann T (2015) Peptide therapeutics: current status and future directions. Drug Discov Today 20:122–128. doi: 10.1016/j.drudis.2014.10.00310.1016/j.drudis.2014.10.003 ↗
19. Hanahan D, Weinberg RA (2011) Hallmarks of cancer: the next generation. Cell 144:646–674. doi: 10.1016/j.cell.2011.02.01310.1016/j.cell.2011.02.013 ↗
20. Hansen L, Larsen EK, Nielsen EH, Iversen F, Liu Z, Thomsen K, Pedersen M, Skrydstrup T, Nielsen NC, Ploug M, Kjems J (2013) Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells. Nano 5:8192–8201. doi: 10.1039/C3NR32922D10.1039/C3NR32922D ↗
21. Hernández B, Carelli C, Coïc YM, De Coninck J, Ghomi M (2009) Vibrational analysis of amino acids and short peptides in aqueous media. V. The effect of the disulfide bridge on the structural features of the peptide hormone somatostatin-14. J Phys Chem B 113:12796–12803. doi: 10.1021/jp904737v10.1021/jp904737v ↗
22. Hernández B, Pflüger F, Adenier A, Kruglik SG, Ghomi M (2010) Vibrational analysis of amino acids and short peptides in hydrated media. VIII. Amino acids with aromatic side chains: l-phenylalanine, l-tyrosine, and l-tryptophan. J Phys Chem B 114:15319–15330. doi: 10.1021/jp106786j10.1021/jp106786j ↗
23. Hoyer D, Bodekke HW (1993) Partial agonists, full agonists, antagonists: dilemmas of definition. Trends Pharmacol Sci 14:270–275. doi: 10.1016/0165-6147(93)90129-810.1016/0165-6147(93)90129-8 ↗
24. Hussain S, Pang Y (2015) Surface geometry of tryptophan adsorbed on gold colloidal nanoparticles. J Mol Struct 1096:121–128. doi: 10.1016/j.molstruc.2015.05.00110.1016/j.molstruc.2015.05.001 ↗
25. Jabir NR, Tabrez S, Ashraf GM, Shakil S, Damanhouri GA, Kamal MA (2012) Nanotechnology-based approaches in anticancer research. Int J Nanomedicine 7:4391–4408. doi: 10.2147/IJN.S3383810.2147/IJN.S33838 ↗
26. Jafari A, Farjami Shayesteh S, Salouti M, Boustani K (2015) Dependence of structural phase transition and lattice strain of Fe3O4 nanoparticles on calcination temperature. Indian J Physics 89:551–560. doi: 10.1007/s12648-014-0627-y10.1007/s12648-014-0627-y ↗
27. Kijewska K, Jarzębińska A, Kowalska J, Jemielity J, Kępińska D, Szczytko J, Pisarek M, Wiktorska K, Stolarski J, Krysiński P, Twardowski A, Mazur M (2013) Magnetic-nanoparticle-decorated Polypyrrole Microvessels: toward encapsulation of mRNA cap analogues. Biomacromolecules 14:1867–1876. doi: 10.1021/bm400250g10.1021/bm400250g ↗
28. Kurouski D, Postiglione T, Deckert-Gaudig T, Deckert V, Lednev IK (2013) Amide I vibrational mode suppression in surface (SERS) and tip (TERS) enhanced Raman spectra of protein specimens. Analyst 138:1665–1673. doi: 10.1039/c2an36478f10.1039/c2an36478f ↗
29. Laakkonen P, Vuorinen K (2010) Homing peptides as targeted delivery vehicles. Integr Biol 2:326–337. doi: 10.1039/c0ib00013b10.1039/c0ib00013b ↗
30. Liu C, Liu D-B, Long G-X, Wang J-F, Mei Q, Hu G-Y, Qiu H, Hu G-Q (2013) Specific targeting of angiogenesis in lung cancer with RGD-conjugated Ultrasmall superparamagnetic iron oxide particles using a 4.7T magnetic resonance scanner. Chin Med J 126:2242–2247. doi: 10.3760/cma.j.issn.0366-6999.2013062810.3760/cma.j.issn.0366-6999.20130628 ↗
31. Lu J, Cheng Y, Zhang G, Tang Y, Dong Z, McElwee KJ, Li G (2015) Increased expression of neuropilin 1 in melanoma progression and its prognostic significance in patients with melanoma. Mol Med Rep 12:2668–2676. doi: 10.3892/mmr.2015.375210.3892/mmr.2015.3752 ↗
32. Maiti N, Thomas S, Jacob JA, Chadha R, Mukherjee T, Kapoor S (2012) DFT and surface-enhanced Raman scattering study of tryptophan–silver complex. J Colloid Interf Sci 380:141–149. doi: 10.1016/j.jcis.2012.04.07110.1016/j.jcis.2012.04.071 ↗
33. Majewski P, Krysinski P (2008) Synthesis, surface modifications, and size-sorting of mixed nickel–zinc ferrite colloidal magnetic nanoparticles. Chem Eur J 14:7961–7968. doi: 10.1002/chem.20080036910.1002/chem.200800369 ↗
34. McCarthy SA, Davies GL, Gun’ko YK (2012) Preparation of multifunctional nanoparticles and their assemblies. Nat Protoc 7:1677–1693. doi: 10.1038/nprot.2012.08210.1038/nprot.2012.082 ↗
35. McMahon G (2000) VEGF receptor signaling in tumor angiogenesis. Oncologist 5:3–10. doi: 10.1634/theoncologist.5-suppl_1-310.1634/theoncologist.5-suppl_1-3 ↗
36. Millan A, Urtizberea A, Silva NJO, Palacio F, Amaral VS, Snoeck E, Serin V (2007) Surface effects in maghemite nanoparticles. J Magn Magn Mater 312:L5–L9. doi: 10.1016/j.jmmm.2006.09.01110.1016/j.jmmm.2006.09.011 ↗
37. Mody VV, Cox A, Shah S, Singh A, Bevins W, Parihar H (2014) Magnetic nanoparticle drug delivery systems for targeting tumor. Appl Nanosci 4:385–392. doi: 10.1007/s13204-013-0216-y10.1007/s13204-013-0216-y ↗
38. Nawara K, Romiszewski J, Kijewska K, Szczytko J, Twardowski A, Mazur M, Krysinski P (2012) Adsorption of doxorubicin onto citrate-stabilized magnetic nanoparticles. J Phys Chem C 116:5598–5609. doi: 10.1021/jp209527810.1021/jp2095278 ↗
39. Nieciecka D, Królikowska A, Kijewska K, Blanchard GJ, Krysiński P (2016) Hydrophilic iron oxide nanoparticles probe the organization of biomimetic layers: electrochemical and spectroscopic evidence. Electrochim Acta 209:671–681. doi: 10.1016/j.electacta.2016.05.09810.1016/j.electacta.2016.05.098 ↗
40. Olejnik P, Pawłowska A, Pałys B (2013) Application of polarization modulated infrared reflection absorption spectroscopy for electrocatalytic activity studies of laccase adsorbed on modified gold electrodes. Electrochim Acta 110:105–111. doi: 10.1016/j.electacta.2013.03.08910.1016/j.electacta.2013.03.089 ↗
41. Ota S, Yamazaki N, Tomitaka A, Yamada T, Takemura Y (2014) Hyperthermia using antibody-conjugated magnetic nanoparticles and its enhanced effect with cryptotanshinone. Nano 4:319–330. doi: 10.3390/nano402031910.3390/nano4020319 ↗
42. Panieri E, Santoro MM (2016) ROS homeostasis and metabolism: a dangerous liason in cancer cells. Cell Death Dis 7:e2253. doi: 10.1038/cddis.2016.10510.1038/cddis.2016.105 ↗
43. Prasad NK, Gohri V, Bahadur DJ (2011) Nanoparticles of pure and substituted maghemites (gamma-MxFe2-xO3 where M = Al, Cr, Mn, Zn and 0 ≤ X ≤1.3): a comparative study. J Nanosci Nanotechnol 11:2710–2716. doi: 10.1166/jnn.2011.269410.1166/jnn.2011.2694 ↗
44. Samant RS, Shevde LA (2011) Recent advances in anti-angiogenic therapy of cancer. Oncotarget 2:122–134. doi: 10.18632/oncotarget.23410.18632/oncotarget.234 ↗
45. Sciacca B, Alvarez SD, Gobaldo F, Sailor MJ (2010) Bioconjugate functionalization of thermally carbonized porous silicon using a radical coupling reaction. Dalton Trans 39:10847–10853. doi: 10.1039/C0DT00936A10.1039/C0DT00936A ↗
46. Shevtsov MA, Nikolaev BP, Ryzhov VA, Yakovleva LY, Dobrodumov AV, Marchenko YY, Margulis BA, Pitkin E, Guzhova IV (2015) Brain tumor magnetic targeting and biodistribution of superparamagnetic iron oxide nanoparticles linked with 70-kDa heatshock protein study by nonlinear longitudinal response. J Magn Magn Mater 388:123–134. doi: 10.1016/j.jmmm.2015.04.03010.1016/j.jmmm.2015.04.030 ↗
47. Singh N, Jenkins GJ, Asadi R, Doak SH (2010) Potential toxicity of superparamagnetic iron oxide nanoparticles (SPION). Nano Rev 1:5358–5373. doi: 10.3402/nano.v1i0.535810.3402/nano.v1i0.5358 ↗
48. Siegel R, Ma J, Zou Z, Jemal A (2014) Cancer statistics, 2014. CA Cancer J Clin 64:9–29. doi: 10.3322/caac.2120810.3322/caac.21208 ↗
49. Soker S, Takashima S, Miao HQ, Neufeld G, Klagsbrun M (1998) Neuropilin-1 is expressed by endothelial and tumor cells as an isoform-specific receptor for vascular endothelial growth factor. Cell 92:735–745. doi: 10.1016/S0092-8674(00)81402-610.1016/S0092-8674(00)81402-6 ↗
50. Starzec A, Vassy R, Martin A, Lecouvey M, Di Benedetto M, Crépin M, Perret G (2006) Antiangiogenic and antitumor activities of peptide inhibiting the vascular endothelial growth factor binding to neuropilin-1. Life Sci 79:2370–2381. doi: 10.1016/j.lfs.2006.08.00510.1016/j.lfs.2006.08.005 ↗
51. Starzec A, Ladam P, Vassy R, Badache S, Bouchemal N, Navaza A, Hervé du Penhoat C, Perret GY (2007) Structure–function analysis of the antiangiogenic ATWLPPR peptide inhibiting VEGF165 binding to neuropilin-1 and molecular dynamics simulations of the ATWLPPR/neuropilin-1 complex. Peptides 28:2397–2402. doi: 10.1016/j.peptides.2007.09.01310.1016/j.peptides.2007.09.013 ↗
52. Sun C, Lee JS, Zhang M (2008) Magnetic nanoparticles in MR imaging and drug delivery. Adv Drug Deliv Rev 60:1252–1265. doi: 10.1016/j.addr.2008.03.01810.1016/j.addr.2008.03.018 ↗
53. Varshney D, Verma K, Kumar A (2011) Structural and vibrational properties of ZnxMn1−x Fe2O4(x= 0.0, 0.25, 0.50, 0.75, 1.0) mixed ferrites. Mater Chem Phys 131:413–419. doi: 10.1016/j.matchemphys.2011.09.06610.1016/j.matchemphys.2011.09.066 ↗
54. Veiseh O, Gunn JW, Zhang M (2010) Design and fabrication of magnetic nanoparticles for targeted drug delivery and imaging. Adv Drug Deliv Rev 62:284–304. doi: 10.1016/j.addr.2009.11.00210.1016/j.addr.2009.11.002 ↗
55. Wei F, Zhang D, Halas NJ, Hartgerink JD (2008) Aromatic amino acids providing characteristic motifs in the Raman and SERS spectroscopy of peptides. J Phys Chem B 112:9158–9164. doi: 10.1021/jp802573210.1021/jp8025732 ↗
56. Xu C, Yepez G, Wei Z, Liu F, Bugarin A, Hong Y (2016) Development of dopant-free conductive Bioelastomers. Sci Rep 6:34451. doi: 10.1038/srep3445110.1038/srep34451 ↗
57. Zhang XX, Eden HS, Chen X (2012) Peptides in cancer nanomedicine: drug carriers, targeting ligands and protease substrates. J Control Release 159:2–13. doi: 10.1016/j.jconrel.2011.10.02310.1016/j.jconrel.2011.10.023 ↗
58. Zhu G, Zhu X, Fan Q, Wan X (2011) Raman spectra of amino acids and their aqueous solutions. Spectrochim Acta A Mol Biomol Spectrosc 78:1187–1195. doi: 10.1016/j.saa.2010.12.07910.1016/j.saa.2010.12.079 ↗