Low cytotoxicity fluorescent PAMAM dendrimer as gene carriers for monitoring the delivery of siRNA
Source abstract
Abstract
Visual detection of gene vectors has attracted a great deal of attention due to the application of these vectors in monitoring and evaluating the effect of gene carriers in living cells. A non-viral vector, the fluorescent PAMAM dendrimer (F-PAMAM), was synthesized through conjugation of PAMAM dendrimers and fluorescein. In vitro and ex vivo experiments show that F-PAMAM exhibits superphotostability, low cytotoxicity and facilitates endocytosis by A549 cells. The vector has a high siRNA binding affinity and it increases the efficiency of cy5-siRNA delivery in A549 cells, in comparison with a cy5-siRNA monomer. Our results provide a new method for simultaneously monitoring the delivery of siRNA and its non-viral carriers in living cells.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: RNA Interference and Gene Delivery · Advanced biosensing and bioanalysis techniques · Dendrimers and Hyperbranched Polymers
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (21)
Show source-provided references
1. Caminade AM, Hameau A, Majoral JP (2009) Multicharged and/or water-soluble fluorescent dendrimers: properties and uses. Chem Eur J15:9270–928510.1002/chem.200901597 ↗
2. Chen Y, Zhou L, Pang Y, Huang W, Qiu F, Jiang X, Zhu X, Yan D, Chen Q (2011) Photoluminescent hyperbranched poly(amido amine) containing β-cyclodextrin as a nonviral gene delivery vector. Bioconjugate Chem 22:1162–117010.1021/bc200010w ↗
3. De Laporte L, Cruz Rea J, Shea LD (2006) Design of modular non-viral gene therapy vectors. Biomaterials 27:947–95410.1016/j.biomaterials.2005.09.036 ↗
4. Edelstein ML, Abedi MR, Wixon J (2007) Gene therapy clinical trials worldwide to 2007—an update. J Gen Intern Med 9:833–84210.1002/jgm.1100 ↗
5. High KA (2011) Gene therapy for haemophilia: a long and winding road. J Thromb Haemost 9:2–1110.1111/j.1538-7836.2011.04369.x ↗
6. Jones CF, Campbell RA, Franks Z, Gibson CC, Thiagarajan G, Vieira-de-Abreu A, Sukavaneshvar S, Mohammad SF, Li DY, Ghandehari H, Weyrich AS, Brooks BD, Grainger DW (2012) Cationic PAMAM dendrimers disrupt key platelet functions. Mol Pharm 9:1599–161110.1021/mp2006054 ↗
7. Kulkarni A, DeFrees K, Hyun SH, Thompson DH (2012) Pendant polymer:amino-β-cyclodextrin:siRNA guest: host nanoparticles as efficient vectors for gene silencing. J Am Chem Soc 134:7596–759910.1021/ja300690j ↗
8. Leng ZH, Zhuang QF, Li YC, He Z, Chen Z, Huang SP, Jia HY, Zhou JW, Liu Y, Du LB (2013) Polyamidoamine dendrimer conjugated chitosan nanoparticles for the delivery of methotrexate. Carbohydr Polym 98:1173–117810.1016/j.carbpol.2013.07.021 ↗
9. Majoros IJ, Myc A, Thomas T, Mehta CB, Baker JR (2006) PAMAM dendrimer-based multifunctional conjugate for cancer therapy: synthesis, characterization, and functionality. Biomacromolecules 7:572–57910.1021/bm0506142 ↗
10. Mellet CO, Fernandez JMG, Benito JM (2011) Cyclodextrin-based gene delivery systems. Chem Soc Rev 40:1586–160810.1039/C0CS00019A ↗
11. Mintzer MA, Simanek EE (2008) Nonviral vectors for gene delivery. Chem Rev 109:259–30210.1021/cr800409e ↗
12. Patri AK, Myc A, Beals J, Thomas TP, Bander NH, Baker JR (2004) Synthesis and in vitro testing of J591 antibody-dendrimer conjugates for targeted prostate cancer therapy. Bioconjugate Chem 15:1174–118110.1021/bc0499127 ↗
13. Ravizzin G, Turkbey B, Barrett T, Kobayashi H, Choyke PL (2009) Nanoparticles in sentinel lymph node mapping. Wiley Interdiscip Rev 1:610–623
14. Sheridan C (2011) Gene therapy finds its niche. Nat Biotechnol 29:121–12810.1038/nbt.1769 ↗
15. Stein CA (1996) Phosphorothioate antisense oligodeoxynucleotides: questions of specificity. Trends Biotechnol 14:147–14910.1016/0167-7799(96)20006-X ↗
16. Urban-Klein B, Werth S, Abuharbeid S, Czubayko F, Aigner A (2004) RNAi-mediated gene-targeting through systemic application of polyethylenimine (PEI)-complexed siRNA in vivo. Gene Ther 12:461–46610.1038/sj.gt.3302425 ↗
17. Wang H, Zhou G, Gai H, Chen X (2012) A fluorescein-based probe with high selectivity to cysteine over homocysteine and glutathione. Chem Commun 48:8341–834310.1039/c2cc33932c ↗
18. Xu Z, He B, Shen J, Yang W, Yin M (2013) Fluorescent water-soluble perylenediimide-cored cationic dendrimers: synthesis, optical properties, and cell uptake. Chem Commun 49:3646–364810.1039/c3cc40330k ↗
19. Yang W, Pan CY, Liu XQ, Wang J (2011) Multiple functional hyperbranched poly(amido amine) nanoparticles: synthesis and application in cell imaging. Biomacromolecules 12:1523–153110.1021/bm1014816 ↗
20. Yellepeddi VK, Kumar A, Palakurthi S (2009) Surface modified poly(amido)amine dendrimers as diverse nanomolecules for biomedical applications. Expert Opin Drug Del 6:835–85010.1517/17425240903061251 ↗
21. Zhu L, Lu Z, Zhao H (2015) Antitumor mechanisms when pRb and p53 are genetically inactivated. Oncogene 34:4547–455710.1038/onc.2014.399 ↗