Assessment of the cytotoxicity of cerium, tin, aluminum, and zinc oxide nanoparticles on human cells
Source abstract
Abstract
In the course of time, there has been an increased usage of commercial products containing nanoparticles formulated from tin oxide (TNPs), cerium oxide (CNPs), aluminum oxide (ANPs), and zinc oxide (ZNPs). Despite the wide use of metal oxide nanoparticles (MONPs), understanding about their toxicity and mechanism of action are still unclear. In the present study, TNPs, CNPs, ANPs, and ZNPs were produced by the method of chemical synthesis and characterized using X-ray diffraction (XRD), transmission electron microscopy (TEM), and Fourier transform infrared (FTIR) spectroscopy. The effect of these MONPs on human red blood cells (RBCs) and human skin cells (HaCaT) was investigated. In skin cells, these MONPs showed concentration-dependent cytotoxicity. ZNPs downregulated the glutathione and upregulated lipid peroxidation and lactate dehydrogenase activity which correlates with the increased reactive oxygen production in cells. Genotoxic effect of ZNPs was measured by comet tail assay that showed increased DNA damage in skin cells. Upregulation of Hsp70, Grp 78, and CYP1A1 was observed following ZNPs and ANPs treatment with skin cells. In the case of CNPs and TNPs, the changes were not significant. This study demonstrates the toxic effects of ZNPs and ANPs on skin cells, suggesting caution must be exercised in their use which may be restricted in the cosmetics industry and also products related to human use.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanoparticles: synthesis and applications · Advanced Nanomaterials in Catalysis · Gold and Silver Nanoparticles Synthesis and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (45)
Show source-provided references
1. Ali D, Alarifi S, Alkahtani S, AlKahtane AA, Almalik A (2015) Cerium oxide nanoparticles induce oxidative stress and genotoxicity in human skin melanoma cells. Cell Biochem Biophy 71(3):1643–1651. https://doi.org/10.1007/s12013-014-0386-610.1007/s12013-014-0386-6 ↗
2. Alshatwi AA, Subbarayan PV, Ramesh E, Al-Hazzani AA, Alsaif MA, Alwarthan AA (2013) Aluminium oxide nanoparticles induce mitochondrial-mediated oxidative stress and alter the expression of antioxidant enzymes in human mesenchymal stem cells. Food Additiv Contamin: Part A 30(1):1–10. https://doi.org/10.1080/19440049.2012.72916010.1080/19440049.2012.729160 ↗
3. AshaRani PV, Low Kah Mun G, Hande MP, Valiyaveettil S (2008) Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano 30;3(2):279–290. https://doi.org/10.1021/nn800596w10.1021/nn800596w ↗
4. Auffan M, Rose J, Wiesner MR, Bottero JY (2009) Chemical stability of metallic nanoparticles: a parameter controlling their potential cellular toxicity in vitro. Environ Pollut 157(4):1127–1133. https://doi.org/10.1016/j.envpol.2008.10.00210.1016/j.envpol.2008.10.002 ↗
5. Aula S, Lakkireddy S, Swamy AV, Kapley A, Jamil K, Tata NR, Hembram K (2014) Biological interactions in vitro of zinc oxide nanoparticles of different characteristics. Mater Res Express 1(3):035041. https://doi.org/10.1088/2053-1591/1/3/03504110.1088/2053-1591/1/3/035041 ↗
6. Banerjee A, Pathak S, Vimala DS, Vasan D, Murugesan R, Verma RS (2017) Strategies for targeted drug delivery in treatment of colon cancer: current trends and future perspectives. Drug Discov Today 22:1224–1232. https://doi.org/10.1016/j.drudis.2017.05.00610.1016/j.drudis.2017.05.006 ↗
7. Banu AS, Devi SV, Prakash T (2013a) Interaction of sol–gel derived TiO2- and SiO2-based bionanocomposites with erythrocytes and serum proteins. IET Nanobiotechnol 7(1):22–27. https://doi.org/10.1049/iet-nbt.2012.000610.1049/iet-nbt.2012.0006 ↗
8. Banu AS, Devi SV, Prakash T (2013b) Interaction of sol–gel derived TiO2-and SiO2-based bionanocomposites with erythrocytes and serum proteins. IET Nanobiotechnol 7(1):22–27. https://doi.org/10.1049/iet-nbt.2012.000610.1049/iet-nbt.2012.0006 ↗
9. Buege JA, Aust SD (1972) Microsomal lipid peroxidation. Methods in Enzymol 52:302–310. https://doi.org/10.1016/s0076-6879(78)52032-610.1016/s0076-6879(78)52032-6 ↗
10. Chalew TEA, Schwab KJ (2013) Toxicity of commercially available engineered nanoparticles to Caco-2 and SW480 human intestinal epithelial cells. Cell Biol Toxicol 29(2):101–116. https://doi.org/10.1007/s10565-013-9241-610.1007/s10565-013-9241-6 ↗
11. Colon J, Herrera L, Smith J, Patil S, Komanki C, Kupelian P, Seal S, Jenkins DW, Baker CH (2009) Protection from radiation-induced pneumonitis using cerium oxide nanoparticles. Nanomed Nanotechnol Biol Med 5(2):225–231. https://doi.org/10.1016/j.nano.2008.10.00310.1016/j.nano.2008.10.003 ↗
12. Cory AH, Owen TC, Barltrop JA, Cory JG (1991) Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture. Cancer Commun 3(7):207–212. https://doi.org/10.3727/09553549182087319110.3727/095535491820873191 ↗
13. Devi SV, Prakash T (2013) Kinetics of cisplatin release by in-vitro using poly (D, L-lactide) coated Fe3O4 nanocarriers. IEEE Trans Nanobiosci 12(1):60–63. https://doi.org/10.1109/TNB.2012.223002410.1109/TNB.2012.2230024 ↗
14. Di Virgilio AL, Reigosa M, De Mele MFL (2010) Response of UMR 106 cells exposed to titanium oxide and aluminum oxide nanoparticles. J Biomed Res A 92(1):80–86. https://doi.org/10.1002/jbm.a.3233910.1002/jbm.a.32339 ↗
15. Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82(1):70–77. https://doi.org/10.1016/0003-9861(59)90090-610.1016/0003-9861(59)90090-6 ↗
16. Farahmandjou M, Zarinkamar M, Firoozabadi TP (2016) Synthesis of cerium oxide (CeO2) nanoparticles using simple CO-precipitation method. Rev Mex Fis 62(5):496–499 ISSN 0035-001X
17. Filipe P, Silva JN, Silva R, De Castro JC, Gomes MM, Alves LC, Santus R, Pinheiro T (2009) Stratum corneum is an effective barrier to TiO2 and ZnO nanoparticle percutaneous absorption. Skin Pharmacol Physiol 22(5):266–275. https://doi.org/10.1159/00023555410.1159/000235554 ↗
18. Fu PP, Xia Q, Hwang HM, Ray PC, Yu H (2014) Mechanisms of nanotoxicity: generation of reactive oxygen species. J Food Drug Anal 22(1):64–75. https://doi.org/10.1016/j.jfda.2014.01.00510.1016/j.jfda.2014.01.005 ↗
19. Handy RD, Von der Kammer F, Lead JR, Hassellöv M, Owen R, Crane M (2008) The ecotoxicology and chemistry of manufactured nanoparticles. Ecotoxicol 17(4):287–314. https://doi.org/10.1007/s10646-008-0199-810.1007/s10646-008-0199-8 ↗
20. Karakoti A, Singh S, Dowding JM, Seal S, Self WT et al (2010) Redox-active radical scavenging nanomaterials. Chem Soc Rev 39(11):4422–4432. https://doi.org/10.3390/antiox502001510.3390/antiox5020015 ↗
21. Kim IS, Baek M, Choi SJ (2010) Comparative cytotoxicity of Al2O3, CeO2, TiO2 and ZnO nanoparticles to human lung cells. J Nanosci Nanotechnol 10(5):3453–3458. https://doi.org/10.1166/jnn.2010.234010.1166/jnn.2010.2340 ↗
22. Kruger, Nicholas J (2002) The protein protocols handbook. Humana Press, Totowa, pp 15–2110.1385/1-59259-169-8:15 ↗
23. Li JH, Liu XR, Zhang Y, Tian FF, Zhao GY, Jiang FL, Liu Y (2012) Toxicity of nano zinc oxide to mitochondria. Toxicol Res 1(2):137–144. https://doi.org/10.1039/C2TX20016C10.1039/C2TX20016C ↗
24. Lin YS, Haynes CL (2010) Impacts of mesoporous silica nanoparticle size, pore ordering, and pore integrity on hemolytic activity. J Am Chem Soc 132(13):4834–4842. https://doi.org/10.1021/ja910846q10.1021/ja910846q ↗
25. Mittal S, Pandey AK (2014) Cerium oxide nanoparticles induced toxicity in human lung cells: role of ROS mediated DNA damage and apoptosis. BioMed Res Int 2014:2014–2014. https://doi.org/10.1155/2014/89193410.1155/2014/891934 ↗
26. Mosman T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays. J Immunol Methods 65:55–63. https://doi.org/10.1016/0022-1759(83)90303-410.1016/0022-1759(83)90303-4 ↗
27. Nel A, Xia T, Mädler L, Li N (2006) Toxic potential of materials at the nanolevel. Science 311(5761):622–627. https://doi.org/10.1126/science.111439710.1126/science.1114397 ↗
28. Papageorgiou I, Brown C, Schins R, Singh S, Newson R, Davis S, Fisher J, Ingham E, Case CP (2007) The effect of nano-and micron-sized particles of cobalt–chromium alloy on human fibroblasts in vitro. Biomat. 28(19):2946–2958. https://doi.org/10.1016/j.biomaterials.2007.02.03410.1016/j.biomaterials.2007.02.034 ↗
29. Pierscionek BK, Li Y, Yasseen AA, Colhoun LM, Schachar RA, Chen W (2009) Nanoceria have no genotoxic effect on human lens epithelial cells. Nanotechnol. 21(3):035102. https://doi.org/10.1088/0957-4484/21/3/03510210.1088/0957-4484/21/3/035102 ↗
30. Radziun E, Wilczyńska JD, Książek I, Nowak K, Anuszewska EL, Kunicki A, Ząbkowski T (2011) Assessment of the cytotoxicity of aluminium oxide nanoparticles on selected mammalian cells. Toxicol in Vitro 25(8):1694–1700. https://doi.org/10.1016/j.tiv.2011.07.01010.1016/j.tiv.2011.07.010 ↗
31. Rajiv S, Jerobin J, Saranya V, Nainawat M, Sharma A, Makwana P, Mukherjee A (2016) Comparative cytotoxicity and genotoxicity of cobalt (II, III) oxide, iron (III) oxide, silicon dioxide, and aluminum oxide nanoparticles on human lymphocytes in vitro. Hum Exp Toxicol 35(2):170–183. https://doi.org/10.1177/096032711557920810.1177/0960327115579208 ↗
32. Rio DC, Ares M, Hannon GJ, Nilsen TW (2010) Purification of RNA using TRIzol (TRI reagent). Cold Spring Harb Protoc 6:5439. https://doi.org/10.1101/pdb.prot543910.1101/pdb.prot5439 ↗
33. Seliskar M, Rozman D (2007) Mammalian cytochromes P450—importance of tissue specificity. Biochimica et Biophysica Acta (BBA)-General Subjects 1770(3):458–466. https://doi.org/10.1016/j.bbagen.2006.09.01610.1016/j.bbagen.2006.09.016 ↗
34. Sharma V, Singh SK, Anderson D, Tobin DJ, Dhawan A (2011) Zinc oxide nanoparticle induced genotoxicity in primary human epidermal keratinocytes. J Nanosci Nanotechnol 11(5):3782–3788. https://doi.org/10.1166/jnn.2011.425010.1166/jnn.2011.4250 ↗
35. Siddiqui MA, Ahamed M, Ahmad J, Khan MM, Musarrat J, Al-Khedhairy AA, Alrokayan SA (2012) Nickel oxide nanoparticles induce cytotoxicity, oxidative stress and apoptosis in cultured human cells that is abrogated by the dietary antioxidant curcumin. Food Chem Toxicol 50(3–4):641–647. https://doi.org/10.1016/j.fct.2012.01.01710.1016/j.fct.2012.01.017 ↗
36. Singh P, Nanda A (2014) Enhanced sun protection of nano-sized metal oxide particles over conventional metal oxide particles: an in vitro comparative study. Int J Cosmet Sci 36(3):273–283. https://doi.org/10.1111/ics.1212410.1111/ics.12124 ↗
37. Srikanth K, Mahajan A, Pereira E, Duarte AC, Venkateswara Rao J (2015) J. Aluminium oxide nanoparticles induced morphological changes, cytotoxicity and oxidative stress in Chinook salmon (CHSE-214) cells. J Appl Toxicol 35(10):1133–1140. https://doi.org/10.1002/jat.314210.1002/jat.3142 ↗
38. Tammina SK, Mandal BK, Ranjan S, Dasgupta N (2017) Cytotoxicity study of Piper nigrum seed mediated synthesized SnO2 nanoparticles towards colorectal (HCT116) and lung cancer (A549) cell lines. J Photochem Photobiol B 166:158–168. https://doi.org/10.1016/j.jphotobiol.2016.11.01710.1016/j.jphotobiol.2016.11.017 ↗
39. Vimala Devi S, Ramachndran M, Marotta XFS, Pathak S (2019) Comparative study on anti-proliferative potentials of zinc oxide and aluminium oxide nanoparticles in colon cancer cells. Acta Bio Medica Atenei Parmensis 90(2):241–247. https://doi.org/10.23750/abm.v90i2.693910.23750/abm.v90i2.6939 ↗
40. Vinardell MP, Sordé A, Díaz J, Baccarin T, Mitjans M (2015) Comparative effects of macro-sized aluminum oxide and aluminum oxide nanoparticles on erythrocyte hemolysis: influence of cell source, temperature, and size. J Nanopart Res 17(2):80. https://doi.org/10.1007/s11051-015-2893-910.1007/s11051-015-2893-9 ↗
41. Vinardell M, Llanas H, Marics L, Mitjans M (2017) In vitro comparative skin irritation induced by nano and non-nano zinc oxide. Nanomat 7(3):56. https://doi.org/10.3390/nano703005610.3390/nano7030056 ↗
42. Virgilio AL, Reigosa M, De Mele MF (2010) Response of UMR 106 cells exposed to titanium oxide and aluminum oxide nanoparticles. J Biomed Mater Res A 92(1):80–86. https://doi.org/10.1002/jbm.a.3233910.1002/jbm.a.32339 ↗
43. Wise JP Sr, Goodale BC, Wise SS (2010) Silver nanospheres are cytotoxic and genotoxic to fish cells. Aquat Toxicol 97(1):34–41. https://doi.org/10.1016/j.aquatox.2009.11.01610.1016/j.aquatox.2009.11.016 ↗
44. Yuan JS, Reed A, Chen F, Stewart CN (2010) Statistical analysis of real-time PCR data. BMC Bioinformat 7(1) (2006):85. https://doi.org/10.1186/1471-2105-7-8510.1186/1471-2105-7-85 ↗
45. Zhang L, Jiang H, Selke MWang XJ (2014) Selective cytotoxicity effect of cerium oxide nanoparticles under UV irradiation. J Biomed Nanotechnol 10(2):278–286. https://doi.org/10.1166/jbn.2014.179010.1166/jbn.2014.1790 ↗