Mesoporous silica nanorods toward efficient loading and intracellular delivery of siRNA
Source abstract
Abstract
The technology of RNA interference (RNAi) that uses small interfering RNA (siRNA) to silence the gene expression with complementary messenger RNA (mRNA) sequence has great potential for the treatment of cancer in which certain genes were usually found overexpressed. However, the carry and delivery of siRNA to the target site in the human body can be challenging for this technology to be used clinically to silence the cancer-related gene expression. In this work, rod shaped mesoporous silica nanoparticles (MSNs) were developed as siRNA delivery system for specific intracellular delivery. The rod MSNs with an aspect ratio of 1.5 had a high surface area of 934.28 m2/g and achieved a siRNA loading of more than 80 mg/g. With the epidermal growth factor (EGF) grafted on the surface of the MSNs, siRNA can be delivered to the epidermal growth factor receptor (EGFR) overexpressed colorectal cancer cells with high intracellular concentration compared to MSNs without EGF and lead to survivin gene knocking down to less than 30%.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: RNA Interference and Gene Delivery · Advanced biosensing and bioanalysis techniques · Nanoparticle-Based Drug Delivery
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (47)
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1. Ashley CE, Carnes EC, Epler KE, Padilla DP, Phillips GK, Castillo RE, Wilkinson DC, Wilkinson BS, Burgard CA, Kalinich RM, Townson JL, Chackerian B, Willman CL, Peabody DS, Wharton W, Brinker CJ (2012) Delivery of small interfering RNA by peptide-targeted mesoporous silica nanoparticle-supported lipid bilayers. ACS Nano 6(3):2174–2188. https://doi.org/10.1021/nn204102q10.1021/nn204102q ↗
2. Bhattarai SR, Muthuswamy E, Wani A, Brichacek M, Castaneda AL, Brock SL, Oupicky D (2010) Enhanced gene and siRNA delivery by polycation-modified mesoporous silica nanoparticles loaded with chloroquine. Pharm Res 27(12):2556–2568. https://doi.org/10.1007/s11095-010-0245-010.1007/s11095-010-0245-0 ↗
3. Bonde M, Pontoppidan H, Pepper DS (1992) Direct dye binding—a quantitative assay for solid-phase immobilized protein. Anal Biochem 200(1):195–198. https://doi.org/10.1016/0003-2697(92)90298-L10.1016/0003-2697(92)90298-L ↗
4. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1-2):248–254. https://doi.org/10.1016/0003-2697(76)90527-310.1016/0003-2697(76)90527-3 ↗
5. Chen AM, Zhang M, Wei D, Stueber D, Taratula O, Minko T, He H (2009) Co-delivery of doxorubicin and Bcl-2 siRNA by mesoporous silica nanoparticles enhances the efficacy of chemotherapy in multidrug-resistant cancer cells. Small 5(23):2673–2677. https://doi.org/10.1002/smll.20090062110.1002/smll.200900621 ↗
6. Chen F, Hong H, Zhang Y, Valdovinos HF, Shi S, Kwon GS, Theuer CP, Barnhart TE, Cai W (2013) In vivo tumor targeting and image-guided drug delivery with antibody-conjugated, radiolabeled mesoporous silica nanoparticles. ACS Nano 7(10):9027–9039. https://doi.org/10.1021/nn403617j10.1021/nn403617j ↗
7. Chen Y, Gu H, Zhang DS, Li F, Liu T, Xia W (2014) Highly effective inhibition of lung cancer growth and metastasis by systemic delivery of siRNA via multimodal mesoporous silica-based nanocarrier. Biomaterials 35(38):10058–10069. https://doi.org/10.1016/j.biomaterials.2014.09.00310.1016/j.biomaterials.2014.09.003 ↗
8. Chen L, She X, Wang T, He L, Shigdar S, Duan W, Kong L (2015a) Overcoming acquired drug resistance in colorectal cancer cells by targeted delivery of 5-FU with EGF grafted hollow mesoporous silica nanoparticles. Nano 7(33):14080–14092. https://doi.org/10.1039/c5nr03527a10.1039/c5nr03527a ↗
9. Chen Y, Ai K, Liu J, Sun G, Yin Q, Lu L (2015b) Multifunctional envelope-type mesoporous silica nanoparticles for pH-responsive drug delivery and magnetic resonance imaging. Biomaterials 60:111–120. https://doi.org/10.1016/j.biomaterials.2015.05.00310.1016/j.biomaterials.2015.05.003 ↗
10. Chen S, Tam YY, Lin PJ, Sung MM, Tam YK, Cullis PR (2016) Influence of particle size on the in vivo potency of lipid nanoparticle formulations of siRNA. J Control Release 235:236–244. https://doi.org/10.1016/j.jconrel.2016.05.05910.1016/j.jconrel.2016.05.059 ↗
11. de Fougerolles A, Vornlocher HP, Maraganore J, Lieberman J (2007) Interfering with disease: a progress report on siRNA-based therapeutics. Nat Rev Drug Discov 6(6):443–453. https://doi.org/10.1038/nrd231010.1038/nrd2310 ↗
12. Fang IJ, Slowing II, Wu KCW, Lin VSY, Trewyn BG (2012) Ligand conformation dictates membrane and endosomal trafficking of arginine-glycine-aspartate (RGD)-functionalized mesoporous silica nanoparticles. Chem Eur J 18(25):7787–7792. https://doi.org/10.1002/chem.20120002310.1002/chem.201200023 ↗
13. Finlay J, Roberts CM, Dong J, Zink JI, Tamanoi F, Glackin CA (2015) Mesoporous silica nanoparticle delivery of chemically modified siRNA against TWIST1 leads to reduced tumor burden. Nanomedicine 11(7):1657–1666. https://doi.org/10.1016/j.nano.2015.05.01110.1016/j.nano.2015.05.011 ↗
14. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391(6669):806–811 http://www.nature.com/nature/journal/v391/n6669/suppinfo/391806a0_S1.html . https://doi.org/10.1038/3588810.1038/35888 ↗
15. Gan Q, Dai D, Yuan Y, Qian J, Sha S, Shi J, Liu C (2012) Effect of size on the cellular endocytosis and controlled release of mesoporous silica nanoparticles for intracellular delivery. Biomed Microdevices 14(2):259–270. https://doi.org/10.1007/s10544-011-9604-910.1007/s10544-011-9604-9 ↗
16. Hartono SB, Gu W, Kleitz F, Liu J, He L, Middelberg APJ, Yu C, Lu GQM, Qiao SZ (2012) Poly-l-lysine functionalized large pore cubic mesostructured silica nanoparticles as biocompatible carriers for gene delivery. ACS Nano 6(3):2104–2117. https://doi.org/10.1021/nn203964310.1021/nn2039643 ↗
17. Hartono SB, Yu M, Gu W, Yang J, Strounina E, Wang X, Qiao S, Yu C (2014) Synthesis of multi-functional large pore mesoporous silica nanoparticles as gene carriers. Nanotechnology 25(5):055701. https://doi.org/10.1088/0957-4484/25/5/05570110.1088/0957-4484/25/5/055701 ↗
18. He Q, Shi J (2014) MSN anti-cancer nanomedicines: chemotherapy enhancement, overcoming of drug resistance, and metastasis inhibition. Adv Mater (Deerfield Beach, Fla) 26(3):391–411. https://doi.org/10.1002/adma.20130312310.1002/adma.201303123 ↗
19. He Q, Shi J, Cui X, Zhao J, Chen Y, Zhou J (2009a) Rhodamine B-co-condensed spherical SBA-15 nanoparticles: facile co-condensation synthesis and excellent fluorescence features. J Mater Chem 19(21):3395–3403. https://doi.org/10.1039/B900357F10.1039/b900357f ↗
20. He Q, Zhang Z, Gao Y, Shi J, Li Y (2009b) Intracellular localization and cytotoxicity of spherical mesoporous silica nano- and microparticles. Small 5(23):2722–2729. https://doi.org/10.1002/smll.20090092310.1002/smll.200900923 ↗
21. Hong CA, Nam YS (2014) Functional nanostructures for effective delivery of small interfering RNA therapeutics. Theranostics 4(12):1211–1232. https://doi.org/10.7150/thno.849110.7150/thno.8491 ↗
22. Huang X, Teng X, Chen D, Tang F, He J (2010) The effect of the shape of mesoporous silica nanoparticles on cellular uptake and cell function. Biomaterials 31(3):438–448. https://doi.org/10.1016/j.biomaterials.2009.09.06010.1016/j.biomaterials.2009.09.060 ↗
23. Leung AK, Tam YY, Cullis PR (2014) Lipid nanoparticles for short interfering RNA delivery. Adv Genet 88:71–110. https://doi.org/10.1016/b978-0-12-800148-6.00004-310.1016/b978-0-12-800148-6.00004-3 ↗
24. Li L, Huang X, Liu T, Liu H, Hao N, Chen D, Zhang Y, Li L, Tang F (2012) Overcoming multidrug resistance with mesoporous silica nanorods as nanocarrier of doxorubicin. J Nanosci Nanotechnol 12(6):4458–4466. https://doi.org/10.1166/jnn.2012.619810.1166/jnn.2012.6198 ↗
25. Li X, Chen Y, Wang M, Ma Y, Xia W, Gu H (2013) A mesoporous silica nanoparticle—PEI—Fusogenic peptide system for siRNA delivery in cancer therapy. Biomaterials 34(4):1391–1401. https://doi.org/10.1016/j.biomaterials.2012.10.07210.1016/j.biomaterials.2012.10.072 ↗
26. Liu X, Situ A, Kang Y, Villabroza KR, Liao Y, Chang CH, Donahue T, Nel AE, Meng H (2016) Irinotecan delivery by lipid-coated mesoporous silica nanoparticles shows improved efficacy and safety over liposomes for pancreatic cancer. ACS Nano 10(2):2702–2715. https://doi.org/10.1021/acsnano.5b0778110.1021/acsnano.5b07781 ↗
27. Ma X, Zhao Y, Ng KW, Zhao Y (2013) Integrated hollow mesoporous silica nanoparticles for target drug/siRNA co-delivery. Chem Eur J 19(46):15593–15603. https://doi.org/10.1002/chem.20130273610.1002/chem.201302736 ↗
28. Meng H, Yang S, Li Z, Xia T, Chen J, Ji Z, Zhang H, Wang X, Lin S, Huang C, Zhou ZH, Zink JI, Nel AE (2011) Aspect ratio determines the quantity of mesoporous silica nanoparticle uptake by a small GTPase-dependent macropinocytosis mechanism. ACS Nano 5(6):4434–4447. https://doi.org/10.1021/nn103344k10.1021/nn103344k ↗
29. Meng H, Liong M, Xia T, Li Z, Ji Z, Zink JI, Nel AE (2010) Engineered design of mesoporous silica nanoparticles to deliver doxorubicin and P-glycoprotein siRNA to overcome drug resistance in a cancer cell line. ACS Nano 4(8):4539–4550. https://doi.org/10.1021/nn100690m10.1021/nn100690m ↗
30. Moller K, Muller K, Engelke H, Brauchle C, Wagner E, Bein T (2016) Highly efficient siRNA delivery from core-shell mesoporous silica nanoparticles with multifunctional polymer caps. Nano 8(7):4007–4019. https://doi.org/10.1039/c5nr06246b10.1039/c5nr06246b ↗
31. Morry J, Ngamcherdtrakul W, Gu S, Goodyear SM, Castro DJ, Reda MM, Sangvanich T, Yantasee W (2015) Dermal delivery of HSP47 siRNA with NOX4-modulating mesoporous silica-based nanoparticles for treating fibrosis. Biomaterials 66:41–52. https://doi.org/10.1016/j.biomaterials.2015.07.00510.1016/j.biomaterials.2015.07.005 ↗
32. Na HK, Kim MH, Park K, Ryoo SR, Lee KE, Jeon H, Ryoo R, Hyeon C, Min DH (2012) Efficient functional delivery of siRNA using mesoporous silica nanoparticles with ultralarge pores. Small 8(11):1752–1761. https://doi.org/10.1002/smll.20120002810.1002/smll.201200028 ↗
33. Ngamcherdtrakul W, Morry J, Gu S, Castro DJ, Goodyear SM, Sangvanich T, Reda MM, Lee R, Mihelic SA, Beckman BL, Hu Z, Gray JW, Yantasee W (2015) Cationic polymer modified mesoporous silica nanoparticles for targeted SiRNA delivery to HER2+ breast cancer. Adv Funct Mater 25(18):2646–2659. https://doi.org/10.1002/adfm.20140462910.1002/adfm.201404629 ↗
34. Nooney RI, Thirunavukkarasu D, Chen Y, Josephs R, Ostafin AE (2002) Synthesis of nanoscale mesoporous silica spheres with controlled particle size. Chem Mater 14(11):4721–4728. https://doi.org/10.1021/cm020437110.1021/cm0204371 ↗
35. Oishi M, Nakaogami J, Ishii T, Nagasaki Y (2006) Smart PEGylated gold nanoparticles for the cytoplasmic delivery of siRNA to induce enhanced gene silencing. Chem Lett 35(9):1046–1047. https://doi.org/10.1246/cl.2006.104610.1246/cl.2006.1046 ↗
36. Podesta JE, Al-Jamal KT, Herrero MA, Tian B, Ali-Boucetta H, Hegde V, Bianco A, Prato M, Kostarelos K (2009) Antitumor activity and prolonged survival by carbon-nanotube-mediated therapeutic siRNA silencing in a human lung xenograft model. Small 5:1176–1185. https://doi.org/10.1002/smll.20080157210.1002/smll.200990047 ↗
37. She X, Chen L, Velleman L, Li C, He C, Denman J, Wang T, Shigdar S, Duan W, Kong L (2015) The control of epidermal growth factor grafted on mesoporous silica nanoparticles for targeted delivery. J Mater Chem B 3(29):6094–6104. https://doi.org/10.1039/c5tb00790a10.1039/C5TB00790A ↗
38. She X, Chen L, Velleman L, Li C, Zhu H, He C, Wang T, Shigdar S, Duan W, Kong L (2015) Fabrication of high specificity hollow mesoporous silica nanoparticles assisted by Eudragit for targeted drug delivery. J Colloid Interface Sci 445:151–160. https://doi.org/10.1016/j.jcis.2014.12.05310.1016/j.jcis.2014.12.053 ↗
39. Shen J, Kim HC, Su H, Wang F, Wolfram J, Kirui D, Mai J, Mu C, Ji LN, Mao ZW, Shen H (2014) Cyclodextrin and polyethylenimine functionalized mesoporous silica nanoparticles for delivery of siRNA cancer therapeutics. Theranostics 4(5):487–497. https://doi.org/10.7150/thno.826310.7150/thno.8263 ↗
40. Steinbacher JL, Landry CC (2014) Adsorption and release of siRNA from porous silica. Langmuir 30(15):4396–4405. https://doi.org/10.1021/la402850m10.1021/la402850m ↗
41. Sun W, Fang N, Trewyn B, Tsunoda M, Slowing I, Lin VY, Yeung E (2008) Endocytosis of a single mesoporous silica nanoparticle into a human lung cancer cell observed by differential interference contrast microscopy. Anal Bioanal Chem 391(6):2119–2125. https://doi.org/10.1007/s00216-008-2162-110.1007/s00216-008-2162-1 ↗
42. Tang S, Yin Q, Zhang Z, Gu W, Chen L, Yu H, Huang Y, Chen X, Xu M, Li Y (2014) Co-delivery of doxorubicin and RNA using pH-sensitive poly (β-amino ester) nanoparticles for reversal of multidrug resistance of breast cancer. Biomaterials 35(23):6047–6059. https://doi.org/10.1016/j.biomaterials.2014.04.02510.1016/j.biomaterials.2014.04.025 ↗
43. Winder T, Lenz H-J (2010) Molecular prognostic markers in colon cancer. In: Beauchemin N, Huot J (eds) Metastasis of colorectal cancer, vol 14. Cancer metastasis - biology and treatment. Springer Netherlands, pp 321–341. https://doi.org/10.1007/978-90-481-8833-8_1110.1007/978-90-481-8833-8_11 ↗
44. Xiong L, Bi J, Tang Y, Qiao SZ (2016) Magnetic core-shell silica nanoparticles with large radial mesopores for siRNA delivery. Small 12(34):4735–4742. https://doi.org/10.1002/smll.20160053110.1002/smll.201600531 ↗
45. Yao X, Niu X, Ma K, Huang P, Grothe J, Kaskel S, Zhu Y (2016) Graphene quantum dots-capped magnetic mesoporous silica nanoparticles as a multifunctional platform for controlled drug delivery, magnetic hyperthermia, and photothermal therapy. Small 13(2). https://doi.org/10.1002/smll.20160222510.1002/smll.201602225 ↗
46. Yi Z, Hussain HI, Feng C, Sun D, She F, Rookes JE, Cahill DM, Kong L (2015) Functionalized mesoporous silica nanoparticles with redox-responsive short-chain gatekeepers for agrochemical delivery. ACS Appl Mater Interfaces 7(18):9937–9946. https://doi.org/10.1021/acsami.5b0213110.1021/acsami.5b02131 ↗
47. Zhu J, Tang J, Zhao L, Zhou X, Wang Y, Yu C (2010) Ultrasmall, well-dispersed, hollow siliceous spheres with enhanced endocytosis properties. Small 6(2):276–282. https://doi.org/10.1002/smll.20090163110.1002/smll.200901631 ↗