Synthesis and characterisation of highly fluorescent core–shell nanoparticles based on Alexa dyes
Source abstract
Abstract
Current and future developments in the emerging field of nanobiotechnology are closely linked to the rational design of novel fluorescent nanomaterials, e.g. for biosensing and imaging applications. Here, the synthesis of bright near infrared (NIR)-emissive nanoparticles based on the grafting of silica nanoparticles (SNPs) with 3-aminopropyl triethoxysilane (APTES) followed by covalent attachment of Alexa dyes and their subsequent shielding by an additional silica shell are presented. These nanoparticles were investigated by dynamic light scattering (DLS), transmission electron microscopy (TEM) and fluorescence spectroscopy. TEM studies revealed the monodispersity of the initially prepared and fluorophore-labelled silica particles and the subsequent formation of raspberry-like structures after addition of a silica precursor. Measurements of absolute fluorescence quantum yields of these scattering particle suspensions with an integrating sphere setup demonstrated the influence of dye labelling density-dependent fluorophore aggregation on the signaling behaviour of such nanoparticles.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Gold and Silver Nanoparticles Synthesis and Applications · Mesoporous Materials and Catalysis · Carbon and Quantum Dots Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (48)
Show source-provided references
1. Aslan K, Wu M, Lakowicz RJ, Geddes DC (2007) Fluorescent Core − Shell Ag@SiO2 Nanocomposites for Metal-enhanced fluorescence and single nanoparticle sensing platforms. J Am Chem Soc 129:1524–152510.1021/ja0680820 ↗
2. Bargeron BC (1974) Analysis of intensity correlation spectra of mixtures of polystyrene latex spheres: a comparison of direct least squares fitting with the method of cumulants. J Chem Phys 60:2516–251910.1063/1.1681391 ↗
3. Benezra M, Penate-Medina O, Zanzonico BP, Schaer D, Ow H, Burns A, DeStanchina E, Longo V, Herz E, Iyer S, Wolchok J, Larson MS, Wiesner U, Bradbury SM (2011) Multimodal silica nanoparticles are effective cancer-targeted probes in a model of human melanoma. J Clin Invest 121:2768–278010.1172/JCI45600 ↗
4. Bergna HE, Roberts OW (2006) Colloidal Silica: fundamentals and applications CRC Press. Taylor & Francis Group: Boca Raton 131:895
5. Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanosk BJ, Telford WG, Yue S, Liu J, Cheung C-Y, Chang W, Hirsch JD, Beechem JM, Haugland RP, Haugland RP (2003) Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates. Journal of Histochemistry & Cytochemistry 51(12):1179–118810.1177/002215540305101214 ↗
6. Blaaderen AV, Vrij A (1993) Synthesis and characterization of monodisperse colloidal organo-silica spheres. J Colloid Interface Sci 156:1–1810.1006/jcis.1993.1073 ↗
7. Bringley FB, Penner LP, Wang R, Harder FJ, Harrison JW, Buonemani L (2008) Silica nanoparticles encapsulating near-infrared emissive cyanine dyes. J Colloid Interface Sci 320:132–13910.1016/j.jcis.2007.09.006 ↗
8. Burns A, Ow H, Wiesner U (2006a) Fluorescent core–shell silica nanoparticles: towards ‘‘Lab on a Particle’’ architectures for nanobiotechnology. Chem Soc Rev 35:1028–104210.1039/B600562B ↗
9. Burns A, Sengupta P, Zedayko T, Baird B, Wiesner U (2006b) Core/shell fluorescent silica nanoparticles for chemical sensing: towards single-particle laboratories. Small 2:723–72610.1002/smll.200600017 ↗
10. Changfeng W, Craig S, Jason M (2006) Preparation and encapsulation of highly fluorescent conjugated polymer nanoparticles. Langmuir 22:2956–296010.1021/la060188l ↗
11. Edwards SB, Tudor O, Prossnitz RE, Sklar AL (2004) Flow cytometry for high-throughput, high-content screening. Curr Opin Chem Biol 8:392–39810.1016/j.cbpa.2004.06.007 ↗
12. Estephan GZ, Jaber AJ, Schlenoff BJ (2010) Zwitterion-stabilized silica nanoparticles: toward nonstick nano. Langmuir 26:16884–1688910.1021/la103095d ↗
13. Ethiraj SA, Kharrazi S, Hebalkar N, Urban J, Sainkar RS, Kulkarni KS (2007) Highly photostable dye entrapped core–shell particles. Mater Lett 61:4738–474210.1016/j.matlet.2007.03.030 ↗
14. Grabolle M, Spieles M, Lesnyak V, Gaponik N, Eychmüller A, Resch-Genger U (2009) Determination of the fluorescence quantum yield of quantum dots: suitable procedures and achievable uncertainties. Anal Chem 81:6285–629410.1021/ac900308v ↗
15. Graf P, Mantion A, Haase A, Thünemann FA, Masic A, Meier W, Luch A, Taubert A (2011) Silicification of peptide-coated silver nanoparticles—a biomimetic soft chemistry approach toward chiral hybrid core − shell materials. ACS NANO 5:820–83310.1021/nn102969p ↗
16. Härmä H (2002) Particle technologies in diagnostics technology review. TEKES, National Technology Agency 126
17. Herz E, Ow H, Bonner D, Burnsa A, Wiesner U (2009) Dye structure–optical property correlations in near-infrared fluorescent core-shell silica nanoparticles. J Mater Chem 19:6341–634710.1039/b902286d ↗
18. Herz E, Marchincin T, Connelly L, Bonner D, Burns A, Switalski S, Wiesner U (2010) Relative quantum yield measurements of coumarin encapsulated in core-shell silica nanoparticles. J Fluoresc 20:67–7210.1007/s10895-009-0523-6 ↗
19. Johansson MK, Cook RM (2003) Intramolecular dimers: a new design strategy for fluorescence-quenched probes. Chem Eur J 9:3466–347110.1002/chem.200304941 ↗
20. Kind L, Plamper FA, Göbel R, Mantion A, Müller EHA, Pieles U, Taubert A, Meier W (2009) Silsesquioxane/polyamine nanoparticle-templated formation of star- or raspberry-like silica nanoparticles. Langmuir 25:7109–711510.1021/la900229n ↗
21. Langhals H, Esterbauer JA (2009) Fluorescent silica nanoparticles by silylation. Chem Eur J 15:4793–479610.1002/chem.200802638 ↗
22. Larson DR, Ow H, Vishwasrao HD, Heikal AA, Wiesner U, Webb WW (2008) Silica nanoparticle architecture determines radiative properties of encapsulated fluorophores. Chem Mater 20:2677–268410.1021/cm7026866 ↗
23. Lee EJ, Lee N, Kim T, Kim J, Hyeon T (2011) Multifunctional mesoporous silica nanocomposite nanoparticles for theranostic applications. Acc Chem Res 44:893–90210.1021/ar2000259 ↗
24. Liz-Marzan L, Michael G, Paul M (1996) Synthesis of nanosized gold − silica core − shell particles. Langmuir 12:4329–433510.1021/la9601871 ↗
25. Martini M, Montagna M, Ou M, Tillement O, Roux S, Perriat P (2009) How to measure quantum yields in scattering media: application to the quantum yield measurement of fluorescein molecules encapsulated in sub-100 nm silica particles. J Appl Phys 106:09430410.1063/1.3248302 ↗
26. Meldal M (2002) The one-bead two-compound assay for solid phase screening of combinatorial libraries. Biopolymers 66:93–10010.1002/bip.10229 ↗
27. Miletto I, Gilardino A, Zamburlin P, Dalmazzo S, Lovisolo D, Caputo G, Viscard G, Martra G (2010) Highly bright and photostable cyanine dye-doped silica nanoparticles for optical imaging: photophysical characterization and cell tests. Dyes Pigments 84:121–12710.1016/j.dyepig.2009.07.004 ↗
28. Murdock CR, Braydich-Stolle L, Schrand MA, Schlager JJ, M. Hussain MS (2008) Characterization of nanomaterial dispersion in solution prior to in vitro exposure using dynamic light scattering technique. Toxicol Sci 101:239–25310.1093/toxsci/kfm240 ↗
29. Nataliya P-V, Rosaria PH, Janell B-S, Mahesh KB, Paul JM, Fei M, Wai-Y L, Richard PH (1999) Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J Histochem Cytochem 47:1179–118810.1177/002215549904700910 ↗
30. Nooney IR, McCahey NMC, Stranik O, Guevel LX, McDonagh C, MacCraith DB (2009) Experimental and theoretical studies of the optimisation of fluorescence from near-infrared dye-doped silica nanoparticles. Anal Bioanal Chem 393:1143–114910.1007/s00216-008-2418-9 ↗
31. Orts-Gil G, Losik M, Schlaad H, Drechsler M, Hellweg T (2008) Properties of pH-responsive mixed aggregates of polystyrene-block-poly(l-lysine) and nonionic surfactant in solution and adsorbed at a solid surface. Langmuir 24:12823–1282810.1021/la8017562 ↗
32. Ow H, Larson RD, Srivastava M, Baird AB, Webb WW, Weisner U (2005) Bright and stable core − shell fluorescent silica nanoparticles. Nano Lett 5:113–11710.1021/nl0482478 ↗
33. Pyrz DW, Buttrey JD (2008) Particle size determination using TEM: a discussion of image acquisition and analysis for the novice microscopist. Langmuir 24:11350–1136010.1021/la801367j ↗
34. Resch-Genger U, Pfeifer D, Monte C, Pilz W, Hoffmann A, Spieles M, Rurack K, Hollandt J, Taubert D, Schonenberger B, Nording P (2005) Traceability in fluorometry: part II. Spectral fluorescence standards. J Fluoresc 15:315–33610.1007/s10895-005-2629-9 ↗
35. Resch-Genger U, Grabolle M, Cavaliere-Jaricot S, Nitschke R, Nann T (2008) Quantum dots versus organic dyes as fluorescent labels. Nat Methods 5:763–77510.1038/nmeth.1248 ↗
36. Russin JT, Altinoglu IE, Adair HJ, Eklund CP (2010) Measuring the fluorescent quantum efficiency of indocyanine green encapsulated in nanocomposite particulates. J Phys Condens Matter 22:33421710.1088/0953-8984/22/33/334217 ↗
37. Schiestel T, Brunner H, Tovar MEG (2004) Controlled surface functionalization of silica nanospheres by covalent conjugation reactions and preparation of high density streptavidin nanoparticles. J Nanosci Nanotechnol 4:504–51110.1166/jnn.2004.079 ↗
38. Schobel U, Egelhaaf H-J, Brecht A, Oelkrug D, Gauglitz G (1999) New donor − acceptor pair for fluorescent immunoassays by energy transfer. Bioconjug Chem 10:1107–111410.1021/bc990073b ↗
39. Schulz-Ekloff G, Wöhrle D, Duffel VB, Schoonheydt AR (2002) Chromophores in porous silicas and minerals: preparation and optical properties. Microporous and Mesoporous Mater 51:91–13810.1016/S1387-1811(01)00455-3 ↗
40. Sokolov I, Naik S (2008) Novel fluorescent silica nanoparticles: towards ultrabright silica nanoparticles. Small 4:934–93910.1002/smll.200700236 ↗
41. Stöber W, Fink A, Bohn E (1968) Controlled growth of monodisperse silica spheres in the micron size range. J Colloid Interface Sci 26:62–6910.1016/0021-9797(68)90272-5 ↗
42. Sun G, Berezin MY, Fan J, Lee H, Ma J, Zhang K, Wooley KL, Achilefu S (2010) Bright fluorescent nanoparticles for developing potential optical imaging contrast agents. Nanoscale 2:548–55810.1039/b9nr00304e ↗
43. Thomassen JCL, Aerts A, Rabolli V, Lison D, Gonzalez L, Kirsch-Volders M, Napierska D, Hoet HP, Kirschhock AEC, Martens AJ (2010) Synthesis and characterization of stable monodisperse silica nanoparticle sols for in vitro cytotoxicity testing. Langmuir 26:328–33510.1021/la902050k ↗
44. Vasilis N, Christoph B, Ralph W (2003) Fluorescence imaging with near-infrared light: new technological advances that enable in vivo molecular imaging. Eur Radiol 13:195–20810.1007/s00330-002-1524-x ↗
45. Wang L, Chaoyong Y, Weihong T (2005) Dual-luminophore-doped silica nanoparticles for multiplexed signaling. Nano Lett 5:37–4310.1021/nl048417g ↗
46. Weissleder R (2001) A clearer vision for in vivo imaging. Nat Biotechnol 19(4):316–31710.1038/86684 ↗
47. Würth C, Lochmann C, Spieles M, Pauli J, Hoffmann K, Schuttrigkeit T, Franzl T, Resch-Genger U (2009) Evaluation of a commercial integrating sphere setup for the determination of absolute photoluminescence quantum yields of dilute dye solutions. Appl Spectroscopy 64:733–74110.1366/000370210791666390 ↗
48. Würth C, Grabolle M, Pauli J, Spieles M, Resch-Genger U (2011) Comparison of methods and achievable uncertainties for the relative and absolute measurement of photoluminescence quantum yields. Anal Chem 83(9):3431–343910.1021/ac2000303 ↗