The impact of arginine-modified chitosan–DNA nanoparticles on the function of macrophages
Source abstract
Abstract
It has been demonstrated that incorporation of arginine moieties into chitosan significantly elevates the transgenic efficacy of the chitosan. However, little is known about the impact of arginine-modified chitosan on the function of macrophages, which play a vitally important role in the inflammatory response of the body to foreign substances, especially particulate substances. This study was designed to investigate the impact of arginine-modified chitosan/DNA nanoparticles on the function of the murine macrophage through observation of phagocytic activity and production of pro-inflammatory cytokines (IL-1β, IL-6, IL-10, IL-12, and TNF-α). Results showed that both chitosan/DNA nanoparticles and arginine-modified chitosan/DNA nanoparticles, containing 20 μg/mL DNA, were internalized by almost all the macrophages in contact. This led to no significant changes, compared to the non-exposure group, in production of cytokines and phagocytic activity of the macrophages 24 h post co-incubation, whereas exposure to LPS induced obviously elevated cytokine production and phagocytic activity, suggesting that incorporation of arginine moieties into chitosan does not have a negative impact on the function of the macrophages.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: RNA Interference and Gene Delivery · Advanced Drug Delivery Systems · Infant Nutrition and Health
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (34)
Show source-provided references
1. Agnihotri SA, Mallikarjuna NN, Aminabhavi TM (2004) Recent advances on chitosan-based micro- and nanoparticles in drug delivery. J Control Release 100:5–2810.1016/j.jconrel.2004.08.010 ↗
2. Anderson EJ, McGrath MA, Thalhamer T et al (2006) Interleukin-12 to interleukin ‘infinity’: the rationale for future therapeutic cytokine targeting. Springer Semin Immunopathol 27:425–44210.1007/s00281-006-0011-x ↗
3. Binder H, Lindblom G (2004) A molecular view on the interaction of the trojan peptide penetratin with the polar interface of lipid bilayers. Biophys J 87:332–34310.1529/biophysj.103.034025 ↗
4. Bloomfield VA (1996) DNA condensation. Curr Opin Struct Biol 6:334–34110.1016/S0959-440X(96)80052-2 ↗
5. Cardarelli F, Serresi M, Bizzarri R et al (2007) In vivo study of HIV-1 Tat arginine-rich motif unveils its transport properties. Mol Ther 15:1313–132210.1038/sj.mt.6300172 ↗
6. Chellat F, Grandjean-Laquerriere A, Le Naour R et al (2005) Metalloproteinase and cytokine production by THP-1 macrophages following exposure to chitosan-DNA nanoparticles. Biomaterials 26:961–97010.1016/j.biomaterials.2004.04.006 ↗
7. Corsi K, Chellat F, Yahia L et al (2003) Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles. Biomaterials 24:1255–126410.1016/S0142-9612(02)00507-0 ↗
8. Cui Z, Patel J, Tuzova M et al (2004) Strong T cell type-1 immune responses to HIV-1 Tat (1–72) protein-coated nanoparticles. Vaccine 22:2631–264010.1016/j.vaccine.2003.12.013 ↗
9. Erbacher P, Zou S, Bettinger T et al (1998) Chitosan-based vector/DNA complexes for gene delivery: biophysical characteristics and transfection ability. Pharm Res 15:1332–133910.1023/A:1011981000671 ↗
10. Gupta B, Torchilin VP (2006) Transactivating transcriptional activator-mediated drug delivery. Expert Opin Drug Deliv 3:177–19010.1517/17425247.3.2.177 ↗
11. Johnston CJ, Finkelstein JN, Mercer P et al (2000) Pulmonary effects induced by ultrafine PTFE particles. Toxicol Appl Pharmacol 168:208–21510.1006/taap.2000.9037 ↗
12. Kagan VE, Bayir H, Shvedova AA (2005) Nanomedicine and nanotoxicology: two sides of the same coin. Nanomedicine 1:313–31610.1016/j.nano.2005.10.003 ↗
13. Kanchan V, Panda AK (2007) Interactions of antigen-loaded polylactide particles with macrophages and their correlation with the immune response. Biomaterials 28:5344–535710.1016/j.biomaterials.2007.08.015 ↗
14. Kaplan IM, Wadia JS, Dowdy SF (2005) Cationic TAT peptide transduction domain enters cells by macropinocytosis. J Control Release 102:247–25310.1016/j.jconrel.2004.10.018 ↗
15. Liu WG, Yao KD (2002) Chitosan and its derivatives—a promising non-viral vector for gene transfection. J Control Release 83:1–1110.1016/S0168-3659(02)00144-X ↗
16. Liu WG, Zhang JR, Cao ZQ et al (2004) A chitosan-arginine conjugate as a novel anticoagulation biomaterial. J Mater Sci: Mater Med 15:1199–1203
17. Liu LX, Song CN, Song LP et al (2009) Effects of alkylated-chitosan-DNA nanoparticles on the function of macrophages. J Mater Sci: Mater Med 20:943–948
18. MacLaughlin FC, Mumper RJ, Wang J et al (1998) Chitosan and depolymerized chitosan oligomers as condensing carriers for in vivo plasmid delivery. J Control Release 56:259–27210.1016/S0168-3659(98)00097-2 ↗
19. Mansouri S, Lavigne P, Corsi K et al (2004) Chitosan-DNA nanoparticles as non-viral vectors in gene therapy: strategies to improve transfection efficacy. Eur J Pharm Biopharm 57:1–810.1016/S0939-6411(03)00155-3 ↗
20. Mao HQ, Roy K, Troung-Le VL et al (2001) Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency. J Control Release 70:399–42110.1016/S0168-3659(00)00361-8 ↗
21. Mege JL, Meghari S, Honstettre A et al (2006) The two faces of interleukin 10 in human infectious diseases. Lancet Infect Dis 6:557–56910.1016/S1473-3099(06)70577-1 ↗
22. Metselaar JM, Bruin P, de Boer LW et al (2003) A novel family of l-amino acid-based biodegradable polymer-lipid conjugates for the development of long-circulating liposomes with effective drug-targeting capacity. Bioconjug Chem 14:1156–116410.1021/bc0340363 ↗
23. Moghimi SM, Muir IS, Illum L et al (1993) Coating particles with a block co-polymer (poloxamine-908) suppresses opsonization but permits the activity of dysopsonins in the serum. Biochim Biophys Acta 1179:157–16510.1016/0167-4889(93)90137-E ↗
24. Richardson SC, Kolbe HV, Duncan R (1999) Potential of low molecular mass chitosan as a DNA delivery system: biocompatibility, body distribution and ability to complex and protect DNA. Int J Pharm 178:231–24310.1016/S0378-5173(98)00378-0 ↗
25. Rothen-Rutishauser B, Muhlfeld C, Blank F et al (2007) Translocation of particles and inflammatory responses after exposure to fine particles and nanoparticles in an epithelial airway model. Part Fibre Toxicol 4:910.1186/1743-8977-4-9 ↗
26. Sayes CM, Reed KL, Warheit DB (2007) Assessing toxicity of fine and nanoparticles: comparing in vitro measurements to in vivo pulmonary toxicity profiles. Toxicol Sci 97:163–18010.1093/toxsci/kfm018 ↗
27. Takeuchi H, Kojima H, Yamamoto H et al (2001) Evaluation of circulation profiles of liposomes coated with hydrophilic polymers having different molecular weights in rats. J Control Release 75:83–9110.1016/S0168-3659(01)00368-6 ↗
28. Taylor A, Verhagen J, Blaser K et al (2006) Mechanisms of immune suppression by interleukin-10 and transforming growth factor-beta: the role of T regulatory cells. Immunology 117:433–44210.1111/j.1365-2567.2006.02321.x ↗
29. Whiteman KR, Subr V, Ulbrich K et al (2001) Poly(Hpma)-coated liposomes demonstrate prolonged circulation in mice. J Liposome Res 11:153–16410.1081/LPR-100108459 ↗
30. Woodle MC, Lasic DD (1992) Sterically stabilized liposomes. Biochim Biophys Acta 1113:171–19910.1016/0304-4157(92)90038-C ↗
31. Zahr AS, Davis CA, Pishko MV (2006) Macrophage uptake of core-shell nanoparticles surface modified with poly(ethylene glycol). Langmuir 22:8178–818510.1021/la060951b ↗
32. Zhang HL, Zhu DW, Yang J et al (2006) Cellular uptake and cytotoxicity of modified chitosans as gene carriers. Zhongguo Yi Xue Ke Xue Yuan Xue Bao 28:486–491
33. Zheng C, Brownlie R, Babiuk LA et al (2005) Characterization of the nuclear localization and nuclear export signals of bovine herpesvirus 1 VP22. J Virol 79:11864–1187210.1128/JVI.79.18.11864-11872.2005 ↗
34. Zhu DW, Zhang HL, Bo JG et al (2007) Enhancement of transfection efficiency for HeLa cells via incorporating arginine moiety into chitosan. Chin Sci Bull 52:3207–321510.1007/s11434-007-0455-y ↗