Surfactant-assisted dispersion of carbon nanotubes: mechanism of stabilization and biocompatibility of the surfactant
Source abstract
Abstract
Nanoparticles (NPs) are thermodynamically unstable system and tend to aggregate to reduce free energy. The aggregation property of NPs results in inhomogeneous exposure of cells to NPs resulting in variable cellular responses. Several types of surfactants are used to stabilize NP dispersions and obtain homogenous dispersions. However, the effects of these surfactants, per se, on cellular responses are not completely known. The present study investigated the application of Pluronic F68 (PF68) for obtaining stable dispersion of NPs using carbon nanotubes as model NPs. PF68-stabilized NP suspensions are stable for long durations and do not show signs of aggregation or settling during storage or after autoclaving. The polyethylene oxide blocks in PF68 provide steric hindrance between adjacent NPs leading to stable NP dispersions. Further, PF68 is biocompatible in nature and does not affect integrity of mitochondria, lysosomes, DNA, and nuclei. Also, PF68 neither induce free radical or cytokine production nor does it interfere with cellular uptake mechanisms. The results of the present study suggest that PF68-assisted dispersion of NPs produced suspensions, which are stable after autoclaving. Further, PF68 does not interfere with normal physiological functions suggesting its application in nanomedicine and nanotoxicity evaluation.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanoparticles: synthesis and applications · Graphene and Nanomaterials Applications · Carbon Nanotubes in Composites
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (56)
Show source-provided references
1. Antaris AL, Seo JW, Green AA, Hersam MC (2010) Sorting single-walled carbon nanotubes by electronic type using nonionic, biocompatible block copolymers. ACS Nano 4:4725–473210.1021/nn101363m ↗
2. Asati A, Santra S, Kaittanis C, Perez J (2010) Surface-charge-dependent cell localization and cytotoxicity of cerium oxide nanoparticles. ACS Nano 4:5321–533110.1021/nn100816s ↗
3. Bacic D, LeHir M, Biber J, Kaissling B, Murer H, Wagner CA (2006) The renal Na+/phosphate cotransporter NaPi-IIa is internalized via the receptor-mediated endocytic route in response to parathyroid hormone. Kidney Int 69:495–50310.1038/sj.ki.5000148 ↗
4. Batrakova EV, Kabanov AV (2008) Pluronic block copolymers: evolution of drug delivery concept from inert nanocarriers to biological response modifiers. J Control Release 130:98–10610.1016/j.jconrel.2008.04.013 ↗
5. Bihari P, Vippola M, Schultes S, Praetner M, Khandoga AG, Reichel CA, Coester C, Tuomi T, Rehberg M, Krombach F (2008) Optimized dispersion of nanoparticles for biological in vitro and in vivo studies. Part Fibre Toxicol 5:1410.1186/1743-8977-5-14 ↗
6. Blanch AJ, Lenehan CE, Quinton JS (2010) Optimizing surfactant concentrations for dispersion of single-walled carbon nanotubes in aqueous solution. J Phys Chem B 114:9805–981110.1021/jp104113d ↗
7. Buono C, Anzinger JJ, Amar M, Kruth HS (2009) Fluorescent pegylated nanoparticles demonstrate fluid-phase pinocytosis by macrophages in mouse atherosclerotic lesions. J Clin Invest 119:1373–138110.1172/JCI35548 ↗
8. Chen J, Hessler JA, Putchakayala K, Panama BK, Khan DP, Hong S, Mullen DG, DiMaggio SC, Som A, Tew GN, Lopatin AN, Baker JR, Holl MMB, Orr BG (2009) Cationic nanoparticles induce nanoscale disruption in living cell plasma membranes. J Phys Chem B 113:11179–1118510.1021/jp9033936 ↗
9. Cherukuri P, Gannon CJ, Leeuw TK, Schmidt HK, Smalley RE, Curley SA, Weisman RB (2006) Mammalian pharmacokinetics of carbon nanotubes using intrinsic near-infrared fluorescence. Proc Natl Acad Sci 103:18882–1888610.1073/pnas.0609265103 ↗
10. Ciofani G, Raffa V, Pensabene V, Menciassi A, Dario P (2009) Dispersion of multi-walled carbon nanotubes in aqueous pluronic F127 solutions for biological applications. Fuller Nanotub Car N 17:11–2510.1080/15363830802515840 ↗
11. Crouzier T, Nimmagadda A, Nollert MU, McFetridge PS (2008) Modification of single walled carbon nanotube surface chemistry to improve aqueous solubility and enhance cellular interactions. Langmuir 24:13173–1318110.1021/la801999n ↗
12. de Planque MR, Aghdaei S, Roose T, Morgan H (2011) Electrophysiological characterization of membrane disruption by nanoparticles. ACS Nano 5:3599–360610.1021/nn103320j ↗
13. Dong L, Joseph KL, Witkowski CM, Craig MM (2008) Cytotoxicity of single-walled carbon nanotubes suspended in various surfactants. Nanotechnology 19:255702/255701–255702/25570510.1088/0957-4484/19/25/255702 ↗
14. Duan WH, Wang Q, Collins F (2011) Dispersion of carbon nanotubes with SDS surfactants: a study from a binding energy perspective. Chem Sci 2:1407–141310.1039/c0sc00616e ↗
15. Elgrabli D, Abella-Gallart S, Aguerre-Chariol O, Robidel F, Rogerieux F, Boczkowski J, Lacroix G (2007) Effect of BSA on carbon nanotube dispersion for in vivo and in vitro studies. Nanotoxicology 1:266–27810.1080/17435390701775136 ↗
16. Florent M, Shvartzman-Cohen R, Goldfarb D, Yerushalmi-Rozen R (2008) Self-assembly of pluronic block copolymers in aqueous dispersions of single-wall carbon nanotubes as observed by spin probe EPR. Langmuir 24:3773–377910.1021/la7037922 ↗
17. Gil PR, Oberdorster G, Elder A, Puntes V, Parak WJ (2010) Correlating physico-chemical with toxicological properties of nanoparticles: the present and the future. ACS Nano 4:5527–553110.1021/nn1025687 ↗
18. Granite M, Radulescu A, Pyckhout-Hintzen W, Cohen Y (2011) Interactions between block copolymers and single-walled carbon nanotubes in aqueous solutions: a small-angle neutron scattering study. Langmuir 27:751–75910.1021/la103096n ↗
19. Guo YY, Zhang J, Zheng YF, Yang J, Zhu XQ (2011) Cytotoxic and genotoxic effects of multi-wall carbon nanotubes on human umbilical vein endothelial cells in vitro. Mutat Res 721:184–19110.1016/j.mrgentox.2011.01.014 ↗
20. Harush-Frenkel O, Rozentur E, Benita S, Altschuler Y (2008) Surface charge of nanoparticles determines their endocytic and transcytotic pathway in polarized MDCK cells. Biomacromolecules 9:435–44310.1021/bm700535p ↗
21. Hirano S, Kanno S, Furuyama A (2008) Multi-walled carbon nanotubes injure the plasma membrane of macrophages. Toxicol Appl Pharmacol 232:244–25110.1016/j.taap.2008.06.016 ↗
22. Inacio AS, Mesquita KA, Baptista M, Ramalho-Santos J, Vaz WLC, Vieira OV (2011) In vitro surfactant structure-toxicity relationships: implications for surfactant use in sexually transmitted infection prophylaxis and contraception. PLoS One 6:e1985010.1371/journal.pone.0019850 ↗
23. Jain AK, Swarnakar NK, Godugu C, Singh RP, Jain S (2011a) The effect of the oral administration of polymeric nanoparticles on the efficacy and toxicity of tamoxifen. Biomaterials 32:503–51510.1016/j.biomaterials.2010.09.037 ↗
24. Jain AK, Swarnakar NK, Godugu C, Singh RP, Poduri RR, Jain S (2011b) Augmented anticancer efficacy of doxorubicin loaded polymeric nanoparticles after oral administration in breast cancer induced animal model. Mol Pharm 8:1140–115110.1021/mp200011f ↗
25. Kabanov AV, Alakhov VY (2002) Pluronic block copolymers in drug delivery: from micellar nanocontainers to biological response modifiers. Crit Rev Ther Drug Carrier Syst 19:1–7310.1615/CritRevTherDrugCarrierSyst.v19.i1.10 ↗
26. Kawski A, Kuten E, Kaminski J (1973) Fluorescence quenching and nonradiative energy transfer in solutions. J Phys B 6:1907–191610.1088/0022-3700/6/9/034 ↗
27. Lee JH, Hong SK, Kim JM, Ko WB (2011) Synthesis of gold nanoparticles using pluronic F127NF under microwave irradiation and catalytic effects. J Nanosci Nanotechnol 11:734–73710.1166/jnn.2011.3212 ↗
28. Ma J-H, Guo C, Tang Y-L, Liu H-Z (2007) 1H NMR spectroscopic investigations on the micellization and gelation of PEO–PPO–PEO block copolymers in aqueous solutions. Langmuir 23:9596–960510.1021/la701221f ↗
29. Ma G, Yang J, Zhang L, Song C (2010) Effective antitumor activity of paclitaxel-loaded poly (epsilon-caprolactone)/pluronic F68 nanoparticles after intratumoral delivery into the murine breast cancer model. Anticancer Drugs 21:261–26910.1097/CAD.0b013e32833410a2 ↗
30. Meng H, Xia T, George S, Nel AE (2009) A predictive toxicological paradigm for the safety assessment of nanomaterials. ACS Nano 3:1620–162710.1021/nn9005973 ↗
31. Nam HY, Kwon SM, Chung H, Lee SY, Kwon SH, Jeon H, Kim Y, Park JH, Kim J, Her S, Oh YK, Kwon IC, Kim K, Jeong SY (2009) Cellular uptake mechanism and intracellular fate of hydrophobically modified glycol chitosan nanoparticles. J Control Release 135:259–26710.1016/j.jconrel.2009.01.018 ↗
32. Nativ-Roth E, Shvartzman-Cohen R, Bounioux C, Florent M, Zhang D, Szleifer I, Yerushalmi-Rozen R (2007) Physical adsorption of block copolymers to SWNT and MWNT: a nonwrapping mechanism. Macromolecules 40:3676–368510.1021/ma0705366 ↗
33. Neal JC, Stolnik S, Schacht E, Kenawy ER, Garnett MC, Davis SS, Illum L (1998) In vitro displacement by rat serum of adsorbed radiolabeled poloxamer and poloxamine copolymers from model and biodegradable nanospheres. J Pharm Sci 87:1242–124810.1021/js970462j ↗
34. Norppa H, Catalan J, Falck G, Hannukainen K, Siivola K, Savolainen K (2011) Nano-specific genotoxic effects. J Biomed Nanotechnol 7:1910.1166/jbn.2011.1179 ↗
35. Porter D, Sriram K, Wolfarth M, Jefferson A, Schwegler-Berry D, Andrew M, Castranova V (2008) A biocompatible medium for nanoparticle dispersion. Nanotoxicology 2:144–15410.1080/17435390802318349 ↗
36. Pulskamp K, Diabate S, Krug HF (2007) Carbon nanotubes show no sign of acute toxicity but induce intracellular reactive oxygen species in dependence on contaminants. Toxicol Lett 168:58–7410.1016/j.toxlet.2006.11.001 ↗
37. Raja PMV, Connolley J, Ganesan Gopal P, Ci L, Ajayan Pulickel M, Nalamasu O, Thompson Deanna M (2007) Impact of carbon nanotube exposure, dosage and aggregation on smooth muscle cells. Toxicol Lett 169:51–6310.1016/j.toxlet.2006.12.003 ↗
38. Rawat MK, Jain A, Singh S (2011) Studies on binary lipid matrix based solid lipid nanoparticles of repaglinide: in vitro and in vivo evaluation. J Pharm Sci 100:2366–237810.1002/jps.22435 ↗
39. Raychaudhuri B, Abraham S, Bonfield TL, Malur A, Deb A, DiDonato JA, Kavuru MS, Thomassen MJ (2004) Surfactant blocks lipopolysaccharide signaling by inhibiting both mitogen-activated protein and IkappaB kinases in human alveolar macrophages. Am J Respir Cell Mol Biol 30:228–23210.1165/rcmb.2003-0263OC ↗
40. Ruenraroengsak P, Novak P, Berhanu D, Thorley AJ, Valsami-Jones E, Gorelik J, Korchev YE, Tetley TD (2013) Respiratory epithelial cytotoxicity and membrane damage (holes) caused by amine-modified nanoparticles. Nanotoxicology 6:94–10810.3109/17435390.2011.558643 ↗
41. Shajahan AN, Timblin BK, Sandoval R, Tiruppathi C, Malik AB, Minshall RD (2004) Role of Src-induced dynamin-2 phosphorylation in caveolae-mediated endocytosis in endothelial cells. J Biol Chem 279:20392–2040010.1074/jbc.M308710200 ↗
42. Singh RP, Ramarao P (2012) Cellular uptake, intracellular trafficking and cytotoxicity of silver nanoparticles. Toxicol Lett 213:249–25910.1016/j.toxlet.2012.07.009 ↗
43. Singh RP, Das M, Thakare V, Jain S (2012) Functionalization density dependent toxicity of oxidized multiwalled carbon nanotubes in a murine macrophage cell line. Chem Res Toxicol 25:2127–213710.1021/tx300228d ↗
44. Sriram MI, Kanth SB, Kalishwaralal K, Gurunathan S (2010) Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model. Int J Nanomed 5:753–762
45. Srivastava RK, Pant AB, Kashyap MP, Kumar V, Lohani M, Jonas L, Rahman Q (2011) Multi-walled carbon nanotubes induce oxidative stress and apoptosis in human lung cancer cell line-A549. Nanotoxicology 5:195–20710.3109/17435390.2010.503944 ↗
46. Strupp W, Weidinger G, Scheller C, Ehret R, Ohnimus H, Girschick H, Tas P, Flory E, Heinkelein M, Jassoy C (2000) Treatment of cells with detergent activates caspases and induces apoptotic cell death. J Membr Biol 175:181–18910.1007/s002320001066 ↗
47. Teodoro JS, Simoes AM, Duarte FV, Rolo AP, Murdoch RC, Hussain SM, Palmeira CM (2011) Assessment of the toxicity of silver nanoparticles in vitro: a mitochondrial perspective. Toxicol In Vitro 25:664–67010.1016/j.tiv.2011.01.004 ↗
48. Terman A, Kurz T, Navratil M, Arriaga EA, Brunk UT (2010) Mitochondrial turnover and aging of long-lived postmitotic cells: the mitochondrial-lysosomal axis theory of aging. Antioxid Redox Signal 12:503–53510.1089/ars.2009.2598 ↗
49. Thakare V, Das M, Jain AK, Patil S, Jain S (2010) Carbon nanotubes in cancer theragnosis. Nanomedicine 5:1277–130110.2217/nnm.10.95 ↗
50. Vankoningsloo S, Piret JP, Saout C, Noel F, Mejia J, Zouboulis CC, Delhalle J, Lucas S, Toussaint O (2010) Cytotoxicity of multi-walled carbon nanotubes in three skin cellular models: effects of sonication, dispersive agents and corneous layer of reconstructed epidermis. Nanotoxicology 4:84–9710.3109/17435390903428869 ↗
51. Wang L, Liu Y, Li W, Jiang X, Ji Y, Wu X, Xu L, Qiu Y, Zhao K, Wei T, Li Y, Zhao Y, Chen C (2011) Selective targeting of gold nanorods at the mitochondria of cancer cells: implications for cancer therapy. Nano Lett 11:772–78010.1021/nl103992v ↗
52. Warheit DB, Reed KL, Sayes CM (2009) A role for nanoparticle surface reactivity in facilitating pulmonary toxicity and development of a base set of hazard assays as a component of nanoparticle risk management. Inhal Toxicol 21:61–6710.1080/08958370902942640 ↗
53. Wick P, Manser P, Limbach LK, Dettlaff-Weglikowska U, Krumeich F, Roth S, Stark WJ, Bruinink A (2007) The degree and kind of agglomeration affect carbon nanotube cytotoxicity. Toxicol Lett 168:121–13110.1016/j.toxlet.2006.08.019 ↗
54. Xing Y, Xiong W, Zhu L, Osawa E, Hussin S, Dai L (2011) DNA damage in embryonic stem cells caused by nanodiamonds. ACS Nano 5:2376–238410.1021/nn200279k ↗
55. Xu Y, Liu BR, Lee HJ, Shannon KB, Winiarz JG, Wang TC, Chiang HJ, Huang YW (2010) Nona-arginine facilitates delivery of quantum dots into cells via multiple pathways. J Biomed Biotechnol 2010:948543
56. Zhong Y, Yingge Z, Yanlian Y, Lan S, Dong H, Hong L, Chen W (2010) Pharmacological and toxicological target organelles and safe use of single-walled carbon nanotubes as drug carriers in treating Alzheimer disease. Nanomedicine 6:427–44110.1016/j.nano.2009.11.007 ↗