journal article·2012-08

Environmental applications and potential health implications of quantum dots

Farhan Ahmad, Alok K. Pandey, Amanda B. Herzog, Joan B. Rose, Charles P. Gerba, Syed A. Hashsham

Journal of Nanoparticle Research· Volume 14· Issue 8
Publisher page

Abstract

Quantum dots (QDs) are routinely employed for bioimaging applications and detection of pathogens and toxins. Their use as surrogates to study the fate and transport of non-fluorescent nanoparticles is limited due to high cost, detection of limit issues, and lack of sufficient data related to health effects. Systematic studies on the impact of QDs on environment and health may facilitate its safe use for environmental applications. This review summarizes the studies conducted with QDs with a focus on environmental applications and provides toxicity data important to human health.
Source: openaire · Quality: candidate unverified · Source record ↗

Research context

OpenAlex topics: Quantum Dots Synthesis And Properties · Biosensors and Analytical Detection · Advanced biosensing and bioanalysis techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

References (100+)

Show source-provided references
1. Ahmad F, Hashsham SA (2009) Nanosensors. In: Zhang TC, Surampalli RY, Lai KCK, Hu Z, Tyagi RD, Lo IMC (eds) Nanotechnologies for water environment applications. American Society of Civil Engineers, Reston, pp 412–44310.1061/9780784410301.ch13 ↗
2. Ahmad F, Seyrig G, Tourlousse D, Stedtfeld R, Tiedje J, Hashsham S (2011) A CCD-based fluorescence imaging system for real-time loop-mediated isothermal amplification-based rapid and sensitive detection of waterborne pathogens on microchips. Biomed Microdevices 13:929–93710.1007/s10544-011-9562-2 ↗
3. Akerman ME, Chan WCW, Laakkonen P, Bhatia SN, Ruoslahti E (2002) Nanocrystal targeting in vivo. Proc Natl Acad Sci USA 99:12617–1262110.1073/pnas.152463399 ↗
4. Alivisatos AP (1996) Semiconductor clusters, nanocrystals, and quantum dots. Science 271:933–93710.1126/science.271.5251.933 ↗
5. Alivisatos P (2000) Colloidal quantum dots. From scaling laws to biological applications. Pure Appl Chem 72:3–910.1351/pac200072010003 ↗
6. Alivisatos P (2004) The use of nanocrystals in biological detection. Nat Biotechnol 22:47–5210.1038/nbt927 ↗
7. Alivisatos AP, Johnsson KP, Peng XG, Wilson TE, Loweth CJ, Bruchez MP, Schultz PG (1996) Organization of ‘nanocrystal molecules’ using DNA. Nature 382:609–61110.1038/382609a0 ↗
8. Awara WM, El-Nabi SH, El-Gohary M (1998) Assessment of vinyl chloride-induced DNA damage in lymphocytes of plastic industry workers using a single-cell gel electrophoresis technique. Toxicology 128:9–1610.1016/S0300-483X(98)00008-0 ↗
9. Bakalova R, Ohba H, Zhelev Z, Nagase T, Jose R, Ishikawa M, Baba Y (2004) Quantum dot anti-CD conjugates: are they potential photosensitizers or potentiators of classical photosensitizing agents in photodynamic therapy of cancer? Nano Lett 4:1567–157310.1021/nl049627w ↗
10. Bales RC, Hinkle SR, Kroeger TW, Stocking K, Gerba CP (1991) Bacteriophage adsorption during transport through porous-media—chemical perturbations and reversibility. Environ Sci Technol 25:2088–209510.1021/es00024a016 ↗
11. Ballou B, Lagerholm BC, Ernst LA, Bruchez MP, Waggoner AS (2004) Noninvasive imaging of quantum dots in mice. Bioconj Chem 15:79–8610.1021/bc034153y ↗
12. Bao YJ, Li JJ, Wang YT, Yu L, Lou L, Du WJ, Zhu ZQ, Peng H, Zhu JZ (2011) Probing cytotoxicity of CdSe and CdSe/CdS quantum dots. Clin Chem Lett 22:843–84610.1016/j.cclet.2010.12.008 ↗
13. BCC Research of Wellesley (2011) Quantum dots: global market growth and future commercial prospects. http://www.bccresearch.com/report/quantum-dots-market-prospects-nan027c.html
14. Beebe DJ, Mensing GA, Walker GM (2004) Physics and applications of microfluidics in biology. Annu Rev Biomed Eng 4:261–28610.1146/annurev.bioeng.4.112601.125916 ↗
15. Borrebaeck CAK (2000) Antibodies in diagnostics—from immunoassays to protein chips. Immunol Today 21:379–38210.1016/S0167-5699(00)01683-2 ↗
16. Bottini M, Cerignoli F, Dawson MI, Magrini A, Rosato N, Mustelin T (2006) Full-length single-walled carbon nanotubes decorated with streptavidin-conjugated quantum dots as multivalent intracellular fluorescent nanoprobes. Biomacromolecules 7:2259–226310.1021/bm0602031 ↗
17. Bruchez M, Moronne M, Gin P, Weiss S, Alivisatos AP (1998) Semiconductor nanocrystals as fluorescent biological labels. Science 281:2013–201610.1126/science.281.5385.2013 ↗
18. Chan WCW, Nie SM (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281:2016–201810.1126/science.281.5385.2016 ↗
19. Chen FQ, Gerion D (2004) Fluorescent CdSe/ZnS nanocrystal-peptide conjugates for long-term, nontoxic imaging and nuclear targeting in living cells. Nano Lett 4:1827–183210.1021/nl049170q ↗
20. Cho SJ, Maysinger D, Jain M, Roder B, Hackbarth S, Winnik FM (2007) Long-term exposure to CdTe quantum dots causes functional impairments in live cells. Langmuir 23:1974–198010.1021/la060093j ↗
21. Clapp AR, Medintz IL, Uyeda HT, Fisher BR, Goldman ER, Bawendi MG, Mattoussi H (2005) Quantum dot-based multiplexed fluorescence resonance energy transfer. J Am Chem Soc 127:18212–1822110.1021/ja054630i ↗
22. Courty S, Luccardini C, Bellaiche Y, Cappello G, Dahan M (2006) Tracking individual kinesin motors in living cells using single quantum-dot imaging. Nano Lett 6:1491–149510.1021/nl060921t ↗
23. Dabbousi BO, RodriguezViejo J, Mikulec FV, Heine JR, Mattoussi H, Ober R, Jensen KF, Bawendi MG (1997) (CdSe)ZnS core-shell quantum dots: synthesis and characterization of a size series of highly luminescent nanocrystallites. J Phys Chem B 101:9463–947510.1021/jp971091y ↗
24. Dahan M, Laurence T, Pinaud F, Chemla DS, Alivisatos AP, Sauer M, Weiss S (2001) Time-gated biological imaging by use of colloidal quantum dots. Opt Lett 26:825–82710.1364/OL.26.000825 ↗
25. Dahan M, Levi S, Luccardini C, Rostaing P, Riveau B, Triller A (2003) Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking. Science 302:442–44510.1126/science.1088525 ↗
26. Dameron CT, Resse RN, Mehra RK, Kortan AR, Carroll PJ, Steigerwald ML, Brus LE, Winge DR (1989) Biosynthesis of cadmium sulphide quantum semiconductor crystallites. Nature 338:596–59710.1038/338596a0 ↗
27. Darnault CJG, Bonina SMC, Uyusur B, Snee PT (2009) Visualization and transport of quantum dot nanomaterials in porous media. In: Linkov I, Steevens J (eds) Nanomaterials: risks and benefits. Springer, Dordrecht, pp 139–14810.1007/978-1-4020-9491-0_10 ↗
28. Derfus AM, Chan WCW, Bhatia SN (2004) Probing the cytotoxicity of semiconductor quantum dots. Nano Lett 4:11–1810.1021/nl0347334 ↗
29. Dhawan A, Taurozzi JS, Pandey AK, Shan WQ, Miller SM, Hashsham SA, Tarabara VV (2006) Stable colloidal dispersions of C-60 fullerenes in water: evidence for genotoxicity. Environ Sci Technol 40:7394–740110.1021/es0609708 ↗
30. Dixit SK, Goicochea NL, Daniel MC, Murali A, Bronstein L, De M, Stein B, Rotello VM, Kao CC, Dragnea B (2006) Quantum dot encapsulation in viral capsids. Nano Lett 6:1993–199910.1021/nl061165u ↗
31. Domingos RF, Baalousha MA, Ju-Nam Y, Reid MM, Tufenkji N, Lead JR, Leppard GG, Wilkinson KJ (2009) Characterizing manufactured nanoparticles in the environment: multimethod determination of particle sizes. Environ Sci Technol 43:7277–728410.1021/es900249m ↗
32. Dubertret B (2005) Quantum dots—DNA detectives. Nat Mater 4:797–79810.1038/nmat1520 ↗
33. Dubertret B, Skourides P, Norris DJ, Noireaux V, Brivanlou AH, Libchaber A (2002) In vivo imaging of quantum dots encapsulated in phospholipid micelles. Science 298:1759–176210.1126/science.1077194 ↗
34. Eastman PS, Ruan WM, Doctolero M, Nuttall R, De Feo G, Park JS, Chu JSF, Cooke P, Gray JW, Li S, Chen FQF (2006) Qdot nanobarcodes for multiplexed gene expression analysis. Nano Lett 6:1059–106410.1021/nl060795t ↗
35. Fahrenkrog B, Aebi U (2003) The nuclear pore complex: nucleocytoplasmic transport and beyond. Nat Rev Mol Cell Biol 4:757–76610.1038/nrm1230 ↗
36. Fan TWM, Teh SJ, Hinton DE, Higashi RM (2002) Selenium biotransformations into proteinaceous forms by foodweb organisms of selenium-laden drainage waters in California. Aquat Toxicol 57:65–8410.1016/S0166-445X(01)00261-2 ↗
37. Gao XH, Nie SM (2004) Quantum dot-encoded mesoporous beads with high brightness and uniformity: rapid readout using flow cytometry. Anal Chem 76:2406–241010.1021/ac0354600 ↗
38. Gao XH, Qi LF (2008) Emerging application of quantum dots for drug delivery and therapy. Expert Opin Drug Deliv 5:263–26710.1517/17425247.5.3.263 ↗
39. Gao XH, Chan WCW, Nie SM (2002) Quantum-dot nanocrystals for ultrasensitive biological labeling and multicolor optical encoding. J Biomed Opt 7:532–53710.1117/1.1506706 ↗
40. Gaponik N, Talapin DV, Rogach AL, Hoppe K, Shevchenko EV, Kornowski A, Eychmuller A, Weller H (2002) Thiol-capping of CdTe nanocrystals: an alternative to organometallic synthetic routes. J Phys Chem B 106:7177–718510.1021/jp025541k ↗
41. Garaj-Vrhovac V, Zeljezic D (2002) Assessment of genome damage in a population of Croatian workers employed in pesticide production by chromosomal aberration analysis, micronucleus assay and Comet assay. J Appl Toxicol 22:249–25510.1002/jat.855 ↗
42. Geissler D, Charbonniere LJ, Ziessel RF, Butlin NG, Lohmannsroben HG, Hildebrandt N (2010) Quantum dot biosensors for ultrasensitive multiplexed diagnostics. Angew Chem Int Ed 49:1396–140110.1002/anie.200906399 ↗
43. Gerion D, Pinaud F, Williams SC, Parak WJ, Zanchet D, Weiss S, Alivisatos AP (2001) Synthesis and properties of biocompatible water-soluble silica-coated CdSe/ZnS semiconductor quantum dots. J Phys Chem B 105:8861–887110.1021/jp0105488 ↗
44. Gerion D, Chen FQ, Kannan B, Fu AH, Parak WJ, Chen DJ, Majumdar A, Alivisatos AP (2003) Room-temperature single-nucleotide polymorphism and multiallele DNA detection using fluorescent nanocrystals and microarrays. Anal Chem 75:4766–477210.1021/ac034482j ↗
45. Goldman ER, Clapp AR, Anderson GP, Uyeda HT, Mauro JM, Medintz IL, Mattoussi H (2004) Multiplexed toxin analysis using four colors of quantum dot fluororeagents. Anal Chem 76:684–68810.1021/ac035083r ↗
46. Gonzales E, Arbiol J, Puntes VF (2011) Carving at the nanoscale: sequential galvanic exchange and Kirkendall growth at room temperature. Science 334:1377–138010.1126/science.1212822 ↗
47. Goyer RA (1995) Nutrition and metal toxicity. Am J Clin Nutr 61:S646–S65010.1093/ajcn/61.3.646S ↗
48. Green M, Howman E (2005) Semiconductor quantum dots and free radical induced DNA nicking. Chem Commun 121–12310.1039/b413175d ↗
49. Guo WZ, Li JJ, Wang YA, Peng XG (2003) Conjugation chemistry and bioapplications of semiconductor box nanocrystals prepared via dendrimer bridging. Chem Mater 15:3125–313310.1021/cm034341y ↗
50. Guo G, Liu W, Liang J, He Z, Xu H, Yang X (2007) Probing the cytotoxicity of CdSe quantum dots with surface modification. Mater Lett 61:1641–164410.1016/j.matlet.2006.07.105 ↗
51. Guzelian AA, Banin U, Kadavanich AV, Peng X, Alivisatos AP (1996a) Colloidal chemical synthesis and characterization of InAs nanocrystal quantum dots. Appl Phys Lett 69:1432–143410.1063/1.117605 ↗
52. Guzelian AA, Katari JEB, Kadavanich AV, Banin U, Hamad K, Juban E, Alivisatos AP, Wolters RH, Arnold CC, Heath JR (1996b) Synthesis of size-selected, surface-passivated InP nanocrystals. J Phys Chem 100:7212–721910.1021/jp953719f ↗
53. Hahn MA, Keng PC, Krauss TD (2008) Flow cytometric analysis to detect pathogens in bacterial cell mixtures using semiconductor quantum dots. Anal Chem 80:864–87210.1021/ac7018365 ↗
54. Hamilton SJ (2004) Review of selenium toxicity in the aquatic food chain. Sci Total Environ 326:1–3110.1016/j.scitotenv.2004.01.019 ↗
55. Han MY, Gao XH, Su JZ, Nie S (2001) Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules. Nat Biotechnol 19:631–63510.1038/90228 ↗
56. Hanaki K, Momo A, Oku T, Komoto A, Maenosono S, Yamaguchi Y, Yamamoto K (2003) Semiconductor quantum dot/albumin complex is a long-life and highly photostable endosome marker. Biochem Biophys Res Commun 302:496–50110.1016/S0006-291X(03)00211-0 ↗
57. Hardman R (2006) A toxicologic review of quantum dots: toxicity depends on physicochemical and environmental factors. Environ Health Perspect 114:165–17210.1289/ehp.8284 ↗
58. Harrison MT, Kershaw SV, Burt MG, Eychmuller A, Weller H, Rogach AL (2000a) Wet chemical synthesis and spectroscopic study of CdHgTe nanocrystals with strong near-infrared luminescence. Mater Sci Eng B 69:355–36010.1016/S0921-5107(99)00254-8 ↗
59. Harrison MT, Kershaw SV, Burt MG, Rogach AL, Kornowski A, Eychmuller A, Weller H (2000b) Colloidal nanocrystals for telecommunications. Complete coverage of the low-loss fiber windows by mercury telluride quantum dots. Pure Appl Chem 72:295–30710.1351/pac200072010295 ↗
60. Hartmann A, Herkommer K, Gluck M, Speit G (1995) DNA-damaging effect of cyclophosphamide on human blood-cells in vivo and in vitro studied with the single-cell gel test (Comet assay). Environ Mol Mutagen 25:180–18710.1002/em.2850250303 ↗
61. Henson MC, Chedrese PJ (2004) Endocrine disruption by cadmium, a common environmental toxicant with paradoxical effects on reproduction. Exp Biol Med 229:383–39210.1177/153537020422900506 ↗
62. Hetsch F, Xu XQ, Wang HK, Kershaw SV, Rogach AL (2011) Semiconductor nanocrystal quantum dots as solar cell components and photosensitizers: material, charge transfer, and separation aspects of some device topologies. J Phys Chem Lett 2:1879–188710.1021/jz200802j ↗
63. Ho YP, Kung MC, Yang S, Wang TH (2005) Multiplexed hybridization detection with multicolor colocalization of quantum dot nanoprobes. Nano Lett 5:1693–169710.1021/nl050888v ↗
64. Hoet PH, Bruske-Hohlfeld I, Salata OV (2004) Nanoparticles—known and unknown health risks. J Nanobiotechnol 2:1–1510.1186/1477-3155-2-1 ↗
65. Hoshino A, Fujioka K, Oku T, Suga M, Sasaki YF, Ohta T, Yasuhara M, Suzuki K, Yamamoto K (2004a) Physicochemical properties and cellular toxicity of nanocrystal quantum dots depend on their surface modification. Nano Lett 4:2163–216910.1021/nl048715d ↗
66. Hoshino A, Hanaki K, Suzuki K, Yamamoto K (2004b) Applications of T-lymphoma labeled with fluorescent quantum dots to cell tracing markers in mouse body. Biochem Biophys Res Commun 314:46–5310.1016/j.bbrc.2003.11.185 ↗
67. Huang XY, Weng JF, Sang FM, Song XT, Cao CX, Ren JC (2006a) Characterization of quantum dot bioconjugates by capillary electrophoresis with laser-induced fluorescent detection. J Chromatogr A 1113:251–25410.1016/j.chroma.2006.02.087 ↗
68. Huang ZL, Zhao YD, Luo QM (2006b) Quantum-dot-tagged microbeads and their use as fluorescent biological probes. Curr Anal Chem 2:59–6610.2174/157341106775197349 ↗
69. IFT (2002) Institute of food technology expert report on emerging microbiological food safety issues—implications for control in the 21st century. IFT, Washington, DC
70. Ipe BL, Lehnig M, Niemeyer CM (2005) On the generation of free radical species from quantum dots. Small 1:706–70910.1002/smll.200500105 ↗
71. Jaiswal JK, Simon SM (2004) Potentials and pitfalls of fluorescent quantum dots for biological imaging. Trends Cell Biol 14:497–50410.1016/j.tcb.2004.07.012 ↗
72. Jaiswal JK, Mattoussi H, Mauro JM, Simon SM (2003) Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat Biotechnol 21:47–5110.1038/nbt767 ↗
73. Jamieson T, Bakhshi R, Petrova D, Pocock R, Imani M, Seifalian AM (2007) Biological applications of quantum dots. Biomaterials 28:4717–473210.1016/j.biomaterials.2007.07.014 ↗
74. Jans H, Liu X, Austin L, Maes G, Huo Q (2009) Dynamic light scattering as a powerful tool for gold nanoparticle bioconjugation and biomolecular binding studies. Anal Chem 81:9425–943210.1021/ac901822w ↗
75. Jordan A, Scholz R, Wust P, Schirra H, Schiestel T, Schmidt H, Felix R (1999) Endocytosis of dextran and silan-coated magnetite nanoparticles and the effect of intracellular hyperthermia on human mammary carcinoma cells in vitro. J Magn Magn Mater 194:185–19610.1016/S0304-8853(98)00558-7 ↗
76. Kang SH, Bozhilov KN, Myung NV, Mulchandani A, Chen W (2008) Microbial synthesis of CdS nanocrystals in genetically engineered E. coli. Angew Chem Int Edit 47:5186–518910.1002/anie.200705806 ↗
77. Khaliq A, Sonawane PJ, Sasi BK, Sahu BS, Pradeep T, Das SK, Mahapatra NR (2010) Enhancement in the efficiency of polymerase chain reaction by TiO(2) nanoparticles: crucial role of enhanced thermal conductivity. Nanotechnology 21:1–1110.1088/0957-4484/21/25/255704 ↗
78. Kim K, Yoon S, Yoon E, Koo YM, Lee JL (2003a) Aspect ratio dependent strains in InAs/InP quantum dots measured by synchrotron radiation X-ray diffraction. J Vac Sci Technol B 21:183–18510.1116/1.1537713 ↗
79. Kim S, Fisher B, Eisler HY, Bawendi MG (2003b) Novel type-II quantum dots: CdTe/CdSe(core/shell) and CdSe/ZnTe(core/shell) heterostructures. J Am Chem Soc 125:11466–1146710.1021/ja0361749 ↗
80. Kim SC, Harrington MS, Pui DYH (2007) Experimental study of nanoparticles penetration through commercial filter media. J Nanopart Res 9:117–12510.1007/s11051-006-9176-4 ↗
81. Kim YG, Moon S, Kuritzkes DR, Demirci U (2009) Quantum dot-based HIV capture and imaging in a microfluidic channel. Biosens Bioelectron 25:253–25810.1016/j.bios.2009.06.023 ↗
82. Kirchner C, Javier AM, Susha AS, Rogach AL, Kreft O, Sukhorukov GB, Parak WJ (2005a) Cytotoxicity of nanoparticle-loaded polymer capsules. Talanta 67:486–49110.1016/j.talanta.2005.06.042 ↗
83. Kirchner C, Liedl T, Kudera S, Pellegrino T, Javier AM, Gaub HE, Stolzle S, Fertig N, Parak WJ (2005b) Cytotoxicity of colloidal CdSe and CdSe/ZnS nanoparticles. Nano Lett 5:331–33810.1021/nl047996m ↗
84. Kloepfer JA, Mielke RE, Wong MS, Nealson KH, Stucky G, Nadeau JL (2003) Quantum dots as strain- and metabolism-specific microbiological labels. Appl Environ Microbiol 69:4205–421310.1128/AEM.69.7.4205-4213.2003 ↗
85. Klostranec JM, Xiang Q, Farcas GA, Lee JA, Rhee A, Lafferty EI, Perrault SD, Kain KC, Chan WCW (2007) Convergence of quantum dot barcodes with microfluidics and signal processing for multiplexed high-throughput infectious disease diagnostics. Nano Lett 7:2812–281810.1021/nl071415m ↗
86. Kondoh M, Araragi S, Sato K, Higashimoto M, Takiguchi M, Sato M (2002) Cadmium induces apoptosis partly via caspase-9 activation in HL-60 cells. Toxicology 170:111–11710.1016/S0300-483X(01)00536-4 ↗
87. Krichevsky O, Bonnet G (2002) Fluorescence correlation spectroscopy: the technique and its applications. Rep Prog Phys 65:251–29710.1088/0034-4885/65/2/203 ↗
88. Lad AD, Mahamuni S (2008) Effect of ZnS shell formation on the confined energy levels of ZnSe quantum dots. Phys Rev B 78:125421–12542910.1103/PhysRevB.78.125421 ↗
89. Lambertini E, Spencer SK, Bertz PD, Loge FJ, Kieke BA, Borchardt MA (2008) Concentration of Enteroviruses, Adenoviruses, and Noroviruses from drinking water by use of glass wool filters. Appl Environ Microbiol 74:2990–299610.1128/AEM.02246-07 ↗
90. Larson DR, Zipfel W, Clark S, Bruchez M, Wise F, Webb WW (2003) Novel water-soluble quantum dots with large two-photon cross-sections for biological imaging. Biophys J 84:23A
91. Li M, Lin YC, Wu CC, Liu HS (2005) Enhancing the efficiency of a PCR using gold nanoparticles. Nucleic Acids Res 33:e18410.1093/nar/gni183 ↗
92. Li WY, Mei F, He XW, Zhang YK (2008) Preparation and characterization of CdHgTe nanoparticles and their application on the determination of proteins. J Fluoresc 18:883–89010.1007/s10895-008-0318-1 ↗
93. Liang GF, Ma C, Zhu YL, Li SC, Shao YH, Wang Y, Xiao ZD (2011) Enhanced specificity of multiplex polymerase chain reaction via CdTe quantum dots. Nanoscale Res Lett 6:5110.1007/s11671-010-9797-5 ↗
94. Liao YF, Li WJ (2008) Synthesis of CdSe quantum dots via paraffin liquid and oleic acid. J Zhejiang Univ Sci A 9:133–13610.1631/jzus.A071436 ↗
95. Limaye DA, Shaikh ZA (1999) Cytotoxicity of cadmium and characteristics of its transport in cardiomyocytes. Toxicol Appl Pharmacol 154:59–6610.1006/taap.1998.8575 ↗
96. Lin ZB, Cui SX, Zhang H, Chen QD, Yang B, Su XG, Zhang JH, Jin QH (2003) Studies on quantum dots synthesized in aqueous solution for biological labeling via electrostatic interaction. Anal Biochem 319:239–24310.1016/S0003-2697(03)00287-2 ↗
97. Liu WT (2006) Nanoparticles and their biological and environmental applications. J Biosci Bioeng 102:1–710.1263/jbb.102.1 ↗
98. Liu WT, Zhu L, Qin QW, Zhang Q, Feng HH, Ang S (2005) Microfluidic device as a new platform for immunofluorescent detection of viruses. Lab Chip 5:1327–133010.1039/b509086e ↗
99. Liu JH, Fan JB, Gu Z, Cui J, Xu XB, Liang ZW, Luo SL, Zhu MQ (2008) Green chemistry for large-scale synthesis of semiconductor quantum dots. Langmuir 24:5241–524410.1021/la8005986 ↗
100. Lopez E, Figueroa S, Oset-Gasque MJ, Gonzalez MP (2003) Apoptosis and necrosis: two distinct events induced by cadmium in cortical neurons in culture. Br J Pharmacol 138:901–91110.1038/sj.bjp.0705111 ↗