Non-crosslinking gold nanoprobe-LAMP for simple, colorimetric, and specific detection of Salmonella typhi
Source abstract
Abstract
In this study, we developed a non-crosslinking gold nanoprobe loop-mediated isothermal amplification (LAMP) method for nanodiagnosis of bacterial typhoid fever source, Salmonella typhi. Therefore, a unique region in the S. typhi genomic DNA was targeted for LAMP amplification using a specific set of four precisely designed primers. Also, for specific colorimetric visualization of the amplicons, a thiolated oligonucleotide probe, complementary to the single-stranded loop region of the amplicons between F2 and F1C segments, was designed. The probe was bound to the surface of gold nanoparticles via covalent bonds. Increasing the salt concentration in the detection reaction medium led to aggregation of nanoprobes in the blank and the negative vessels in a time-dependent form. That was followed by a change in the surface plasmon resonance (SPR) leading to blue/black color that was observable by the naked eyes after about 5 min. Meanwhile, the original pink/red color was retained in the positive sample due to the large interparticle spaces and the stability against the ionic strength elevation which persisted for about 30 min. The whole process of DNA extraction, amplification, and detection took less than 2 h with a sensitivity of 20 CFU/ml. The developed gold nanoprobe-LAMP could serve as a simple, rapid, and cost-effective method for nanodiagnosis of S. typhi in point-of-need applications.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Biosensors and Analytical Detection · Advanced biosensing and bioanalysis techniques · Advanced Nanomaterials in Catalysis
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (32)
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1. Abdullah J et al (2014) Rapid detection of salmonella typhi by loop-mediated isothermal amplification (LAMP) method. Braz J Microbiol 45:1385–139110.1590/S1517-83822014000400032 ↗
2. Chou P-H et al (2011) Real-time target-specific detection of loop-mediated isothermal amplification for white spot syndrome virus using fluorescence energy transfer-based probes. J Virol Methods 173(1):67–7410.1016/j.jviromet.2011.01.009 ↗
3. Crump JA, Luby SP, Mintz ED (2004) The global burden of typhoid fever. Bull World Health Organ 82(5):346–353
4. Bacchetti De Gregoris, T., et al., Improvement of phylum- and class-specific primers for real-time PCR quantification of bacterial taxa. J Microbiol Methods, 2011. 86(3): p. 351–356.10.1016/j.mimet.2011.06.010 ↗
5. Doria G, Franco R, Baptista P (2007) Nanodiagnostics: fast colorimetric method for single nucleotide polymorphism/mutation detection. Iet Nanobiotechnology 1(4):53–5710.1049/iet-nbt:20070001 ↗
6. Elghanian R (1997) Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles. Science 277(5329):1078–108110.1126/science.277.5329.1078 ↗
7. Fan F et al (2015) The development and evaluation of a loop-mediated isothermal amplification method for the rapid detection of Salmonella enterica serovar typhi. PLoS One 10(4):e012450710.1371/journal.pone.0124507 ↗
8. Hornick RB et al (1970) Typhoid fever: pathogenesis and immunologic control. N Engl J Med 283(14):739–74610.1056/NEJM197010012831406 ↗
9. Iwasaki M et al (2003) Validation of the loop-mediated isothermal amplification method for single nucleotide polymorphism genotyping with whole blood. Genome Letters 2(3):119–12610.1166/gl.2003.028 ↗
10. Lam L et al (2008) Loop-mediated isothermal amplification of a single DNA molecule in polyacrylamide gel-based microchamber. Biomed Microdevices 10(4):539–54610.1007/s10544-008-9163-x ↗
11. Lee MF, Chen YH, Peng CF (2009) Evaluation of reverse transcription loop-mediated isothermal amplification in conjunction with ELISA-hybridization assay for molecular detection of Mycobacterium tuberculosis. J Microbiol Methods 76(2):174–18010.1016/j.mimet.2008.10.005 ↗
12. Li X et al (2009) A loop-mediated isothermal amplification method targets the phoP gene for the detection of Salmonella in food samples. Int J Food Microbiol 133(3):252–25810.1016/j.ijfoodmicro.2009.05.027 ↗
13. Luk JM et al (1997) An enzyme-linked immunosorbent assay to detect PCR products of the rfbS gene from serogroup D salmonellae: a rapid screening prototype. J Clin Microbiol 35(3):714–71810.1128/JCM.35.3.714-718.1997 ↗
14. Misawa Y et al (2007) Application of loop-mediated isothermal amplification technique to rapid and direct detection of methicillin-resistant Staphylococcus aureus (MRSA) in blood cultures. J Infect Chemother 13(3):134–14010.1007/s10156-007-0508-9 ↗
15. Mollasalehi H, Yazdanparast R (2012) Non-crosslinking gold nanoprobes for detection of nucleic acid sequence-based amplification products. Anal Biochem 425(2):91–9510.1016/j.ab.2012.03.008 ↗
16. Mollasalehi H, Yazdanparast R (2013) An improved non-crosslinking gold nanoprobe-NASBA based on 16S rRNA for rapid discriminative bio-sensing of major salmonellosis pathogens. Biosens Bioelectron 47:231–23610.1016/j.bios.2013.03.012 ↗
17. Mori Y, Notomi T (2009) Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. J Infect Chemother 15(2):62–6910.1007/s10156-009-0669-9 ↗
18. Mori Y et al (2001) Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 289(1):150–15410.1006/bbrc.2001.5921 ↗
19. Mori Y et al (2004) Real-time turbidimetry of LAMP reaction for quantifying template DNA. J Biochem Biophys Methods 59(2):145–15710.1016/j.jbbm.2003.12.005 ↗
20. Mori Y, Hirano T, Notomi T (2006) Sequence specific visual detection of LAMP reactions by addition of cationic polymers. BMC Biotechnol 6(1):310.1186/1472-6750-6-3 ↗
21. Nimitphak T, Kiatpathomchai W, Flegel TW (2008) Shrimp hepatopancreatic parvovirus detection by combining loop-mediated isothermal amplification with a lateral flow dipstick. J Virol Methods 154(1):56–6010.1016/j.jviromet.2008.09.003 ↗
22. Noguez C (2007) Surface plasmons on metal nanoparticles: the influence of shape and physical environment. J Phys Chem C 111(10):3806–381910.1021/jp066539m ↗
23. Notomi T et al (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28(12):E6310.1093/nar/28.12.e63 ↗
24. Okamura M et al (2008) Loop-mediated isothermal amplification for the rapid, sensitive, and specific detection of the O9 group of Salmonella in chickens. Vet Microbiol 132(1):197–20410.1016/j.vetmic.2008.04.029 ↗
25. Okamura M et al (2009) Rapid, sensitive, and specific detection of the O4 group of Salmonella enterica by loop-mediated isothermal amplification. Avian Dis 53(2):216–22110.1637/8450-081808-Reg.1 ↗
26. Parry CM et al (2007) Randomized controlled comparison of ofloxacin, azithromycin, and an ofloxacin-azithromycin combination for treatment of multidrug-resistant and nalidixic acid-resistant typhoid fever. Antimicrob Agents Chemother 51(3):819–82510.1128/AAC.00447-06 ↗
27. Parry CM et al (2011) The utility of diagnostic tests for enteric fever in endemic locations. Expert Rev Anti-Infect Ther 9(6):711–72510.1586/eri.11.47 ↗
28. Ravan H, Yazdanparast R (2012) Development and evaluation of a loop-mediated isothermal amplification method in conjunction with an enzyme-linked immunosorbent assay for specific detection of Salmonella serogroup D. Anal Chim Acta 733:64–7010.1016/j.aca.2012.04.034 ↗
29. Rosi NL, Mirkin CA (2005) Nanostructures in biodiagnostics. Chem Rev 105(4):1547–156210.1021/cr030067f ↗
30. Storhoff JJ et al (1998) One-pot colorimetric differentiation of polynucleotides with single base imperfections using gold nanoparticle probes. J Am Chem Soc 120(9):1959–196410.1021/ja972332i ↗
31. Teng PH et al (2007) Specific detection of reverse transcription-loop-mediated isothermal amplification amplicons for Taura syndrome virus by colorimetric dot-blot hybridization. J Virol Methods 146(1–2):317–32610.1016/j.jviromet.2007.07.027 ↗
32. Veigas B et al (2013) Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to rifampicin resistance in Mycobacterium tuberculosis. Journal of Nanobiotechnology 11:38–3810.1186/1477-3155-11-38 ↗