Reconstitution of aluminium and iron core in horse spleen apoferritin
Source abstract
Abstract
This study investigates the uptake of iron and aluminium by apoferritin. In particular, we provide the first evidence that apoferritin is able to bind in vitro the physiological form of aluminium, Al(OH)4 −, to reach an Al/Fe atomic ratio of about 0.15. Mass spectrometry analysis shows that the Al content increases linearly as a function of Al concentration in solution. These findings provide a better understanding of the Al uptake in vivo, confirming that the metal content of ferritin depends on the metal bio-availability.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Trace Elements in Health · Iron Metabolism and Disorders · Heavy Metal Exposure and Toxicity
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References (15)
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1. Amenitsch H, Bernstorff S, Kriechbaum M, Lombardo D, Mio H, Rappolt M, Laggner P (1997) Performance and first results of the ELETTRA high-flux beamline for small-angle x-ray scattering. J Appl Crystallogr 30:872–876. doi: 10.1107/S002188989700159310.1107/S0021889897001593 ↗
2. Douglas T, Ripoll DR (1998) Calculated electrostatic gradients in recombinant human h-chain ferritin. Protein Science 7:1083–1091. doi: 10.1002/pro.556007050210.1002/pro.5560070502 ↗
3. Fleming J, Joshi J (1991) Ferritin: The role of aluminium in ferritin function. Neurobiology of Aging 12(5):413–418. doi: 10.1016/0197-4580(91)90066-S10.1016/0197-4580(91)90066-S ↗
4. Galvetz N, Fernandez B, Sanchez P, Cuesta R, Ceolin M, Clemente-Leon M, Trasobares S, Lopez-Haro M, Calvino JJ, Stephan O, Dominguez-Vera JM (2008) Comparative structural and chemical studies of ferritin cores with gradual removal of their iron contents. J Am Chem Soc 130(25):8062–8068. doi: 10.1021/ja800492z10.1021/ja800492z ↗
5. Grady J (2000) Vanadyl (iv) binding to mammalian ferritins. an epr study aided by site-directed mutagenesis. Journal of Inorganic Biochemistry 80:107–113. doi: 10.1016/S0162-0134(00)00046-5
6. Harrison P (1996) The ferritins: molecular properties, iron storage function and cellular regulation. Biochim Biophys Acta 1275:161–203. doi: 10.1016/0005-2728(96)00022-910.1016/0005-2728(96)00022-9 ↗
7. Joshi J, Sczekan S, Fleming J (1989) Ferritin a general metal detoxicant. Biol Trace Elem Res 21:105–110. doi: 10.1007/BF0291724210.1007/BF02917242 ↗
8. Martin FB (1986) The chemistry of aluminium as related to biology and medicine. Clin Chem 32: 1797–1806. (URL http://www.clinchem.org/cgi/content/abstract/32/10/1797 )10.1093/clinchem/32.10.1797 ↗
9. Spada PL, Rossi C, Alimonti A, Bocca B, Cozza V, Ricerca B, Bocci M, Vulpio C, De Sole P (2008) Ferritin iron content in haemodialysis patients: Comparison with septic and hemochromatosis patients. Clin Biochem 41:997–1001. doi: 10.1016/j.clinbiochem.2008.05.00310.1016/j.clinbiochem.2008.05.003 ↗
10. Spada PL, Rossi C, Alimonti A, Bocca B, Ricerca BM, Bocci MG, Carvelli M, Vulpio C, Lucani G, De Sole P (2009) Iron, zinc and aluminium ferritin content of hemodialysis hyperferritinemic patients: comparison with other hyperferritinemic clinical conditions and normoferritinemic blood donors. Clin Biochem 42:1654–1657. doi: 10.1016/j.clinbiochem.2009.07.02110.1016/j.clinbiochem.2009.07.021 ↗
11. Svergun DI (1992) Determination of the regularization parameter in indirect-transform methods using perceptual criteria. J Appl Crystallogr 25:495–503. doi: 10.1107/S002188989200166310.1107/S0021889892001663 ↗
12. Svergun DI (2001) Determination of domain structure of proteins from x-ray solution scattering. Biophys J 80:2946–2953. doi: 10.1016/S0006-3495(01)76260-110.1016/S0006-3495(01)76260-1 ↗
13. Takahashi T (2003) Functional properties of threefold and fourfold channels in ferritin deduced from electrostatic calculations. Biophys J 84:2256–2263. doi: 10.1016/S0006-3495(03)75031-010.1016/S0006-3495(03)75031-0 ↗
14. Volkov VV, Svergun DI (2003) Uniqueness of ab initio shape determination in small-angle scattering. J Appl Crystallogr 36:860–864. doi: 10.1107/S002188980300026810.1107/S0021889803000268 ↗
15. Yang X, Chasteen N (1996) Molecular diffusion into horse spleen ferritin: a nitroxide radical spin probe study. Biophys J 71:1587–1595. doi: 10.1016/S0006-3495(96)79361-X10.1016/S0006-3495(96)79361-X ↗