Dissolution behavior of metal oxide nanomaterials in cell culture medium versus distilled water
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Abstract
Abstract Solubility is a key criterion used in the hazard assessment of metal oxide–engineered nanomaterials (ENMs). The present study investigated solubility of CuO, NiO, and TiO2ENMs compared with their bulk analogues in two aqueous media: water and Dulbecco’s modified Eagle’s medium (DMEM). Particle size distributions were characterized using dynamic light scattering (DLS) and tunable resistive pulse sensing (TRPS). After centrifugal separation, the dissolved metal fraction was quantified using inductively coupled plasma optical emission spectroscopy (ICP-OES). Overall, solubility of the metal oxides decreased in the order CuO ≥ NiO > TiO2in both media, with each ENM displaying higher solubility than its bulk analogue. However, the metal oxide ENMs responded differently to the two aqueous media, when comparing their solubility using a low initial concentration (10 mg/L) versus a high initial concentration (100 mg/L). In DMEM, both nano-CuO and nano-NiO displayed increased solubility at the higher initial concentration by 3.8-fold and 1.4-fold, respectively. In water, this trend was reversed, with both nano-CuO and nano-NiO displaying increased solubility at the lower initial concentration by 3.3-fold and 1.2-fold, respectively. Interestingly, solubility trends displayed by nano-TiO2were the opposite of those displayed by nano-CuO and nano-NiO. In DMEM, nano-TiO2displayed decreased solubility at the higher initial concentration (0.3-fold), whereas in water, nano-TiO2displayed increased solubility at the higher initial concentration (5.5-fold). These results show the importance of evaluating the solubility of ENMs in biologically relevant fluids at concentrations that correspond to toxicity assays, for the purposes of read-across and grouping ENMs.
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OpenAlex topics: Nanoparticles: synthesis and applications · Healthcare and Environmental Waste Management
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References (67)
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1. Adeleye AS, Conway JR, Perez T, Rutten P, Keller AA (2014) Influence of extracellular polymeric substances on the long-term fate, dissolution, and speciation of copper-based nanoparticles. Environ Sci Technol 48:12561–1256810.1021/es5033426 ↗
2. Alkilany AM, Mahmoud NN, Hashemi F, Hajipour MJ, Farvadi F, Mahmoudi M (2016) Misinterpretation in nanotoxicology: a personal perspective. Chem Res Toxicol 29:943–94810.1021/acs.chemrestox.6b00108 ↗
3. Anderson W, Kozak D, Coleman VA, Jämting TK, Trau M (2013) A comparative study of submicron particle sizing platforms: accuracy, precision and resolution analysis of polydisperse particle size distributions. J Colloid Interface Sci 405:322–33010.1016/j.jcis.2013.02.030 ↗
4. Arts JHE, Hadi M, Irfan M, Keene AM, Kreiling R, Lyon D, Maier M, Michel K, Petry T, Sauer UG, Warheit D, Wiench K, Wohlleben W, Landsiedel R (2015a) A decision-making framework for the grouping and testing of nanomaterials (DF4nanoGrouping). Regul Toxicol Pharmacol 71:S1–S2710.1016/j.yrtph.2015.03.007 ↗
5. Arts JHE, Irfan M, Keene AM, Kreiling R, Lyon D, Maier M, Michel K, Neubauer N, Petry T, Sauer UG, Warheit D, Wiench K, Wohlleben W, Landsiedel R (2015b) Case studies putting the decision-making framework for the grouping and testing of nanomaterials (DF4nanoGrouping) into practice. Regul Toxicol Pharmacol 71:S1–S2710.1016/j.yrtph.2015.03.007 ↗
6. Avramescu M, Chénier M, Gardner HD, Rasmussen PE (2019) Solubility of metal oxide nanomaterials: cautionary notes on sample preparation. J Phys Conf Ser 1323:200110.1088/1742-6596/1323/1/012001 ↗
7. Bove P, Malvindi MA, Kote SS, Bertorelli R, Summa M, Sabella S (2017) Dissolution test for risk assessment of nanoparticles: a pilot study. Nanoscale 9:6315–632610.1039/C6NR08131B ↗
8. Cho W, Duffin R, Howie SEM, Scotton CJ, Wallace WAH, MacNee W, Bradley M, Megson IL, Donaldson K (2011) Progressive severe lung injury by zinc oxide nanoparticles; the role of Zn2+ dissolution inside lysosomes. Part Fibre Toxicol 8:2710.1186/1743-8977-8-27 ↗
9. Cho W, Duffin R, Poland CA, Duschl A, Oostingh GJ, MacNee W, Bradley M, Megson IL, Donaldson K (2012) Differential pro-inflammatory effects of metal oxide nanoparticles and their soluble ions in vitro and in vivo; zinc and copper nanoparticles, but not their ions, recruit eosinophils to the lungs. Nanotoxicology 6:22–3510.3109/17435390.2011.552810 ↗
10. Chowdhury I, Hong Y, Walker SL (2010) Container to characterization: impacts of metal oxide handling, preparation, and solution chemistry on particle stability. Colloids Surf A Physicochem Eng Asp 368:91–9510.1016/j.colsurfa.2010.07.019 ↗
11. Cohen J, Deloid G, Pyrgiotakis G, Demokritou P (2013) Interactions of engineered nanomaterials in physiological media and implications for in vitro dosimetry. Nanotoxicology 7:417–43110.3109/17435390.2012.666576 ↗
12. Decan N, Wu D, Williams A, Bernatchez S, Johnston M, Hill M, Halappanavar S (2016) Characterization of in vitro genotoxic, cytotoxic and transcriptomic responses following exposures to amorphous silica of different sizes. Mutat Res Genet Toxicol Environ Mutagen 796:8–2210.1016/j.mrgentox.2015.11.011 ↗
13. Deloid GM, Cohen JM, Pyrgiotakis G, Demokritou P (2017) Preparation, characterization, and in vitro dosimetry of dispersed, engineered nanomaterials. Nat Protoc 12:355–37110.1038/nprot.2016.172 ↗
14. Duffin R, Tran L, Brown D, Stone V, Donaldson K (2007) Proinflammogenic effects of low-toxicity and metal nanoparticles in vivo and in vitro: highlighting the role of particle surface area and surface reactivity. Inhal Toxicol 19:849–85610.1080/08958370701479323 ↗
15. ECHA 2013 Assessing human health and environmental hazards of nanomaterials—best practice for REACH Registrants 2nd GAARN meeting. ECHA-13-R-04-EN, European Chemicals Agency (ECHA), Helsinki, Finland. In: . https://echa.europa.eu/documents/10162/5399565/best_practices_human_health_environment_nano_en.pdf/8e0adb6a-829c-43aa-84c5-5361f8505996 2019
16. ECHA 2016 Usage of (eco)toxicological data for bridging data gaps between and grouping of nanoforms of the same substance. ED-02-16-228-EN-N, European Chemicals Agency, Joint Research Centre, Dutch National Institute for Public Health and the Environment. In: . https://echa.europa.eu/documents/10162/13630/eco_toxicological_for_bridging_grouping_nanoforms_en.pdf 2019
17. Guggenheim EJ, Milani S, Röttgermann PJF, Dusinska M, Saout C, Salvati A, Rädler JO, Lynch I (2018) Refining in vitro models for nanomaterial exposure to cells and tissues. NanoImpact 10:121–14210.1016/j.impact.2018.02.008 ↗
18. Gunawan C, Teoh WY, Marquis CP, Amal R (2011) Cytotoxic origin of copper(II) oxide nanoparticles: comparative studies with micron-sized particles, leachate, and metal salts. ACS Nano 5:7214–722510.1021/nn2020248 ↗
19. Hansen SF, Hjorth R, Skjolding LM, Bowman DM, Maynard A, Baun A (2017) A critical analysis of the environmental dossiers from the OECD sponsorship programme for the testing of manufactured nanomaterials. Environ Sci Nano 4:282–29110.1039/C6EN00465B ↗
20. Hansjosten I, Rapp J, Reiner L, Vatter R, Fritsch-Decker S, Peravali R, Palosaari T, Joossens E, Gerloff K, Macko P, Whelan M, Gilliland D, Ojea-Jimenez I, Monopoli MP, Rocks L, Garry D, Dawson K, Röttgermann PJF, Murschhauser A, Rädler JO, Tang SVY, Gooden P, Belinga-Desaunay M-A, Khan AO, Briffa S, Guggenheim E, Papadiamantis A, Lynch I, Valsami-Jones E, Diabaté S, Weiss C (2018) Microscopy-based high-throughput assays enable multi-parametric analysis to assess adverse effects of nanomaterials in various cell lines. Arch Toxicol 92:633–64910.1007/s00204-017-2106-7 ↗
21. Hedberg J, Karlsson HL, Hedberg Y, Blomberg E, Odnevall Wallinder I (2016) The importance of extracellular speciation and corrosion of copper nanoparticles on lung cell membrane integrity. Colloids Surf B Biointerfaces 141:291–30010.1016/j.colsurfb.2016.01.052 ↗
22. Hull M, Kennedy AJ, Detzel C, Vikesland P, Chappell MA (2012) Moving beyond mass: the unmet need to consider dose metrics in environmental nanotoxicology studies. Environ Sci Technol 46:10881–1088210.1021/es3035285 ↗
23. ISO/TR 19057:2017 Nanotechnologies: Use and application of acellular in vitro tests and methodologies to assess nanomaterial biodurability. In: . https://www.iso.org/standard/63836.html?browse=tc
24. IZON Scientific How TRPS works. In: . https://izon.com/how-trps-works/ 2019
25. Jensen KA, Kembouche Y, Nielsen SH (2013) Towards a method for detecting the potential genotoxicity of nanomaterials. WP 4: Physicochemical Characterization of Manufactured Nanomaterials (MNs) and Exposure Media (EMs). Deliverable 4.7: Hydrochemical reactivity, solubility, and biodurability of NANOGENOTOX nanomaterials. Key intrinsic physicochemical characteristics of NANOGENOTOX nanomaterials. The National Research Centre for the Working Environment (NRCWE), Lersø Parkallé 105, DK-2100 Copenhagen, DENMARK. . In: . https://www.anses.fr/en/system/files/nanogenotox_deliverable.7.pdf 2019
26. Jeong J, Kim S, Lee S, Lee D, Han Y, Jeon S, Cho W (2018) Differential contribution of constituent metal ions to the cytotoxic effects of fast-dissolving metal-oxide nanoparticles. Front Pharmacol 910.3389/fphar.2018.00015 ↗
27. Jiang J, Oberdörster G, Biswas P (2009) Characterization of size, surface charge, and agglomeration state of nanoparticle dispersions for toxicological studies. J Nanopart Res 11:77–8910.1007/s11051-008-9446-4 ↗
28. Käkinen A, Kahru A, Nurmsoo H, Kubo A, Bondarenko OM (2016) Solubility-driven toxicity of CuO nanoparticles to Caco2 cells and Escherichia coli: effect of sonication energy and test environment. Toxicol Vitro 36:172–17910.1016/j.tiv.2016.08.004 ↗
29. Karlsson HL, Cronholm P, Hedberg Y, Tornberg M, De Battice L, Svedhem S, Wallinder IO (2013) Cell membrane damage and protein interaction induced by copper containing nanoparticles—importance of the metal release process. Toxicology 313:59–6910.1016/j.tox.2013.07.012 ↗
30. Karlsson HL, Gliga AR, Calléja FMGR, Gonçalves CSAG, Wallinder IO, Vrieling H, Fadeel B, Hendriks G (2014) Mechanism-based genotoxicity screening of metal oxide nanoparticles using the ToxTracker panel of reporter cell lines. Part Fibre Toxicol:1110.1186/s12989-014-0041-9 ↗
31. Koltermann-Jülly J, Keller JG, Vennemann A, Werle K, Müller P, Ma-Hock L, Landsiedel R, Wiemann M, Wohlleben W (2018) Abiotic dissolution rates of 24 (nano)forms of 6 substances compared to macrophage-assisted dissolution and in vivo pulmonary clearance: grouping by biodissolution and transformation. NanoImpact 12:29–4110.1016/j.impact.2018.08.005 ↗
32. Krug HF (2014) Nanosafety research-are we on the right track? Angew Chem Int Ed 53:12304–1231910.1002/anie.201403367 ↗
33. Landsiedel R, Ma-Hock L, Wiench K, Wohlleben W, Sauer UG (2017) Safety assessment of nanomaterials using an advanced decision-making framework, the DF4nanoGrouping. J Nanopart Res 1910.1007/s11051-017-3850-6 ↗
34. Latvala S, Hedberg J, Di Bucchianico S, Möller L, Wallinder IO, Elihn K, Karlsson HL (2016) Nickel release, ROS generation and toxicity of Ni and NiO micro- and nanoparticles. PLoS ONE 1110.1371/journal.pone.0159684 ↗
35. Leitner J, Sedmidubský D, Jankovský O (2019) Size and shape-dependent solubility of CuO nanostructures. Mater 1210.3390/ma12203355 ↗
36. Malvern Instruments Ltd (2019a) Dynamic light scattering common terms defined. Inform white paper MRK1764–01:2011. In: http://www.biophysics.bioc.cam.ac.uk/wp-content/uploads/2011/02/DLS_Terms_defined_Malvern.pdf 2019
37. Malvern Instruments Ltd (2019b) Dynamic light scattering: an introduction in 30 minutes. Technical note MRK656–01:2011. In: https://warwick.ac.uk/fac/cross_fac/sciencecity/programmes/internal/themes/am2/booking/particlesize/intro_to_dls.pdf 2019
38. Midander K, Cronholm P, Karlsson HL, Elihn K, Möller L, Leygraf C, Wallinder IO (2009) Surface characteristics, copper release, and toxicity of nano- and micrometer-sized copper and copper(ll) oxide particles: a cross-disciplinary study. Small 5:389–39910.1002/smll.200801220 ↗
39. Minigalieva I, Bushueva T, Fröhlich E, Meindl C, Öhlinger K, Panov V, Varaksin A, Shur V, Shishkina E, Gurvich V, Katsnelson B (2017) Are in vivo and in vitro assessments of comparative and combined toxicity of the same metallic nanoparticles compatible, or contradictory, or both? A juxtaposition of data obtained in respective experiments with NiO and Mn3O4 nanoparticles. Food Chem Toxicol 109:393–40410.1016/j.fct.2017.09.032 ↗
40. Misra SK, Dybowska A, Berhanu D, Luoma SN, Valsami-Jones E (2012a) The complexity of nanoparticle dissolution and its importance in nanotoxicological studies. Sci Total Environ 438:225–23210.1016/j.scitotenv.2012.08.066 ↗
41. Misra SK, Dybowska A, Berhanu D, Croteau MN, Luoma SN, Boccaccini AR, Valsami-Jones E (2012b) Isotopically modified nanoparticles for enhanced detection in bioaccumulation studies. Environ Sci Technol 46:1216–122210.1021/es2039757 ↗
42. Monteiller C, Tran L, MacNee W, Faux S, Jones A, Miller B, Donaldson K (2007) The pro-inflammatory effects of low-toxicity low-solubility particles, nanoparticles and fine particles, on epithelial cells in vitro: the role of surface area. Occup Environ Med 64:609–61510.1136/oem.2005.024802 ↗
43. Nel A, Xia T, Mädler L, Li N (2006) Toxic potential of materials at the nanolevel. Science 311:622–62710.1126/science.1114397 ↗
44. Oberdörster G, Kuhlbusch TAJ (2018) In vivo effects: methodologies and biokinetics of inhaled nanomaterials. NanoImpact 10:38–6010.1016/j.impact.2017.10.007 ↗
45. Odzak N, Kistler D, Behra R, Sigg L (2014) Dissolution of metal and metal oxide nanoparticles in aqueous media. Environ Pollut 191:132–13810.1016/j.envpol.2014.04.010 ↗
46. OECD 1995 (1995) Water Solubility,Test No. 105, Organisation for Economic Co-operation and Development (OECD), Paris, France
47. OECD 2002 (2002) Guidance document on transformation/dissolution of metals and metal compounds in aqueous media. OECD SERIES ON TESTING AND ASSESSMENT number 29, Organisation for Economic Co-operation and Development (OECD), Paris, France
48. OECD 2012 (2012) Guidance on sample preparation and dosimetry for the safety testing of manufactured nanomaterials. Series on the safety of manufactured Nanomaterials no. 3, ENV/JM/MONO(2012)40, Organisation for Economic Co-operation and Development (OECD), Paris, France
49. OECD 2015 (2015) Considerations for using dissolution as a function of surface chemistry to evaluate environmental behaviour of nanomaterials in risk assessments. A preliminary case study using silver nanoparticles
50. Series on the safety of manufactured Nanomaterials, no. 62, ENV/JM/MONO(2015)44, Organisation for Economic Co-operation and Development (OECD), Paris, France
51. OECD 2017 (2017) Dispersion stability of nanomaterials in simulated environmental media. Test no. 318. Organisation for Economic Co-operation and Development (OECD), Paris, France
52. OECD 2018 (2018) Assessment of biodurability of nanomaterials and their surface ligands
53. series on the safety of manufactured nanomaterials no. 86, ENV/JM/MONO(2018)11, Organisation for Economic Co-operation and Development (OECD), Paris, France 2018
54. Pal AK, Aalaei I, Gadde S, Gaines P, Schmidt D, Demokritou P, Bello D (2014) High resolution characterization of engineered nanomaterial dispersions in complex media using tunable resistive pulse sensing technology. ACS Nano 8:9003–901510.1021/nn502219q ↗
55. Peng C, Shen C, Zheng S, Yang W, Hu H, Liu J, Shi J (2017) Transformation of CuO nanoparticles in the aquatic environment: influence of pH, electrolytes and natural organic matter. Nanomaterials 710.3390/nano7100326 ↗
56. Rahman L, Wu D, Johnston M, William A, Halappanavar S (2017) Toxicogenomics analysis of mouse lung responses following exposure to titanium dioxide nanomaterials reveal their disease potential at high doses. Mutagenesis 32:59–7610.1093/mutage/gew048 ↗
57. Semisch A, Ohle J, Witt B, Hartwig A (2014) Cytotoxicity and genotoxicity of nano - and microparticulate copper oxide: role of solubility and intracellular bioavailability. Part Fibre Toxicol 11:1010.1186/1743-8977-11-10 ↗
58. Sohal IS, Cho YK, O’Fallon KS, Gaines P, Demokritou P, Bello D (2018) Dissolution behavior and biodurability of ingested engineered nanomaterials in the gastrointestinal environment. ACS Nano 12:8115–812810.1021/acsnano.8b02978 ↗
59. Strauch BM, Hubele W, Hartwig A (2020) Impact of endocytosis and lysosomal acidification on the toxicity of copper oxide nano-and microsized particles: uptake and gene expression related to oxidative stress and the DNA damage response. Nanomaterials:1010.3390/nano10040679 ↗
60. Studer AM, Limbach LK, Van Duc L, Krumeich F, Athanassiou EK, Gerber LC, Moch H, Stark WJ (2010) Nanoparticle cytotoxicity depends on intracellular solubility: comparison of stabilized copper metal and degradable copper oxide nanoparticles. Toxicol Lett 197:169–17410.1016/j.toxlet.2010.05.012 ↗
61. Taurozzi JS, Hackley VA, Wiesner MR (2011) Ultrasonic dispersion of nanoparticles for environmental, health and safety assessment issues and recommendations. Nanotoxicology 5:711–72910.3109/17435390.2010.528846 ↗
62. Taurozzi JS, Hackley VA, Wiesner MR (2013) A standardised approach for the dispersion of titanium dioxide nanoparticles in biological media. Nanotoxicology 7:389–40110.3109/17435390.2012.665506 ↗
63. Wang Z, Von Dem Bussche A, Kabadi PK, Kane AB, Hurt RH (2013) Biological and environmental transformations of copper-based nanomaterials. ACS Nano 7:8715–872710.1021/nn403080y ↗
64. Wang D, Lin Z, Wang T, Yao Z, Qin M, Zheng S, Lu W (2016) Where does the toxicity of metal oxide nanoparticles come from: the nanoparticles, the ions, or a combination of both? J Hazard Mater 308:328–33410.1016/j.jhazmat.2016.01.066 ↗
65. Wohlleben W, Ma-Hock L, Boyko V, Cox G, Egenolf H, Freiberger H, Hinrichsen B, Hirth S, Landsiedel R (2013) Nanospecific guidance in REACH: a comparative physical-chemical characterization of 15 materials with methodical correlations. J Ceram Sci Technol 4:93–104
66. Yamada M, Takahashi S, Sato H, Kondo T, Kikuchi T, Furuya K, Tanaka I (1993) Solubility of nickel oxide particles in various solutions and rat alveolar macrophages. Biol Trace Elem Res 36:89–9810.1007/BF02783782 ↗
67. Zhang H, Ji Z, Xia T, Meng H, Low-Kam C, Liu R, Pokhrel S, Lin S, Wang X, Liao Y, Wang M, Li L, Rallo R, Damoiseaux R, Telesca D, Mädler L, Cohen Y, Zink JI, Nel AE (2012) Use of metal oxide nanoparticle band gap to develop a predictive paradigm for oxidative stress and acute pulmonary inflammation. ACS Nano 6:4349–436810.1021/nn3010087 ↗