RETRACTED ARTICLE: Development of novel anti-Kv 11.1 antibody-conjugated PEG–TiO2 nanoparticles for targeting pancreatic ductal adenocarcinoma cells
This record is marked as retracted by the source. Review the publisher’s notice before using its findings.
Source abstract
Abstract
Abstract Titanium dioxide (TiO2) has been widely used in many nanotechnology areas including nanomedicine, where it could be proposed for the photodynamic and sonodynamic cancer therapies. However, TiO2 nanoformulations have been shown to be toxic for living cells. In this article, we report the development of a new delivery system, based on nontoxic TiO2 nanoparticles, further conjugated with a monoclonal antibody against a novel and easily accessible tumor marker, e.g., the Kv 11.1 potassium channel. We synthesized, by simple solvothermal method, dicarboxylic acid-terminated PEG TiO2 nanocrystals (PEG–TiO2 NPs). Anti-Kv 11.1 monoclonal antibodies (Kv 11.1-Mab) were further linked to the terminal carboxylic acid groups. Proper conjugation was confirmed by X-ray photoelectron spectroscopy analysis. Kv 11.1-Mab-PEG–TiO2 NPs efficiently recognized the specific Kv 11.1 antigen, both in vitro and in pancreatic ductal adenocarcinoma (PDAC) cells, which express the Kv 11.1 channel onto the plasma membrane. Both PEG TiO2 and Kv 11.1-Mab-PEG–TiO2 NPs were not cytotoxic, but only Kv 11.1-Mab-PEG–TiO2 NPs were efficiently internalized into PDAC cells. Data gathered from this study may have further applications for the chemical design of nanostructures to be applied for therapeutic purposes in pancreatic cancer.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Nanoparticle-Based Drug Delivery · Nanoplatforms for cancer theranostics · Polymer Surface Interaction Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (41)
Show source-provided references
1. Alyaudtin RN, Reichel A, Lobenberg R, Ramge P, Kreuter J, Begley DJ (2001) Interaction of poly(butylcyanoacrylate) nanoparticles with the blood-brain barrier in vivo and in vitro. J Drug Target 9(3):209–22110.3109/10611860108997929 ↗
2. Arcangeli A, Crociani O (2006). Patent FI20,06A,000,008
3. Arcangeli A, Yuan JX (2011) American Journal of Physiology-Cell Physiology theme: ion channels and transporters in cancer. Am J Physiol Cell Physiol 301(2):C253–25410.1152/ajpcell.00159.2011 ↗
4. Ashikaga T, Wada M, Kobayashi H, Mori M, Katsumura Y, Fukui H, Kato S, Yamaguchi M, Takamatsu T (2000) Effect of the photocatalytic activity of TiO2 on plasmid DNA. Mutat Res 466:1–710.1016/S1383-5718(99)00228-4 ↗
5. Caricato AP, Capone S, Ciccarella G, Martino M, Rella R, Romano F, Spadavecchia J, Taurino A, Tunno T, Valerini D (2007) TiO2 nanoparticle thin film deposition by matrix assisted pulsed laser evaporation for sensing applications. Appl Surf Sci 253(19):7937–794110.1016/j.apsusc.2007.02.066 ↗
6. Chenevier P, Veyret B, Roux D, Henry-Toulme N (2000) Interaction of cationic colloids at the surface of J774 cells: a kinetic analysis. Biophys J 79:1298–130910.1016/S0006-3495(00)76383-1 ↗
7. Chithrani BD, Ghazani AA, Chan WC (2006) Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. Nano Lett 6(4):662–66810.1021/nl052396o ↗
8. Ciccarella G, Cingolani R, De Marco L, Gigli G, Melcarne G., Martina F, Matteucci F, Spadavecchia J (2009). World Patent 2009/101640A1
9. D’Amico M, Gasparoli L, Arcangeli A (2013) Potassium channels: novel emerging biomarkers and targets for therapy in cancer. Recent Pat Anticancer Drug Discov 8:53–6510.2174/1574892811308010053 ↗
10. De Marco L, Manca M, Giannuzzi R, Malara F, Melcarne G, Ciccarella G, Zama I, Cingolani R, Gigli G (2010) Novel preparation method of TiO2-nanorod-based photoelectrodes for dye-sensitized solar cells with improved light-harvesting efficiency. J Phys Chem C 114(9):4228–423610.1021/jp910346d ↗
11. Di Paola A, Cufalo G, Addamo M, Bellardita M, Campostrini R, Ischia M, Ceccato R, Palmisano L (2008) Photocatalytic activity of nanocrystalline TiO2 (brookite, rutile and brookite-based) powders prepared by thermohydrolysis of TiCl4 in aqueous chloride solutions. Colloids Surf A 317(1–3):366–37610.1016/j.colsurfa.2007.11.005 ↗
12. Fernandez-Fernandez A, Manchanda R, McGoron AJ (2011) Theranostic applications of nanomaterials in cancer: drug delivery, image-guided. Appl Biochem Biotechnol 165(7–8):1628–165110.1007/s12010-011-9383-z ↗
13. Foley JO, Nelson KE, Mashadi-Hossein A, Finlayson BA, Yager P (2007) Concentration gradient immunoassay. 2. Computational modeling for analysis and optimization. Anal Chem 79(10):3549–355310.1021/ac062350v ↗
14. Grudzinski IP, Bystrzejewski M, Cywinska MA, Kosmider A, Poplawska M, Cieszanowski A, Ostrowska A (2013) Cytotoxicity evaluation of carbon-encapsulated iron nanoparticles in melanoma cells and dermal fibroblasts. J Nanopart Res 15:183510.1007/s11051-013-1835-7 ↗
15. Guasti L, Crociani O, Redaelli E, Pillozzi S, Polvani S, Masselli M, Mello T, Galli A, Amedei A, Wymore RS, Wanke E, Arcangeli A (2008) Identification of a posttranslational mechanism for the regulation of hERG1K+channel expression and hERG1 current density in tumor cells. Mol Cell Biol 28:5043–506010.1128/MCB.00304-08 ↗
16. Huang CC, Aronstam RS, Chen DR, Huang YW (2010) Oxidative stress, calcium homeostasis, and altered gene expression in human lung epithelial cells exposed to ZnO nanoparticles. Toxicol In Vitro 24:45–5510.1016/j.tiv.2009.09.007 ↗
17. Jacobson GB, Gonzalez-Gonzalez E, Spitler R, Shinde R, Leake D, Kaspar RL, Contag CH, Zare RN (2010) Biodegradable nanoparticles with sustained release of functional siRNA in skin. J Pharm Sci 99(10):4261–426610.1002/jps.22147 ↗
18. Jaulin N, Appel M, Passirani C, Barratt G, Labarre D (2000) Reduction of the uptake by a macrophagic cell line of nanoparticles bearing heparin or dextran covalently bound to poly(methyl methacrylate). J Drug Target 8:165–17210.3109/10611860008996862 ↗
19. Jin CY, Zhu BS, Wang XF, Lu QH (2008) Cytotoxicity of titanium dioxide nanoparticles in mouse fibroblast cells. Chem Res Toxicol 21(9):1871–187710.1021/tx800179f ↗
20. Jokerst JV, Lobovkina T, Zare RN, Gambhir SS (2011) Nanoparticle PEGylation for imaging and therapy. Nanomedicine (Lond) 6(4):715–72810.2217/nnm.11.19 ↗
21. Kim BY, Rutka JT, Chan WC (2010) Nanomedicine. N Engl J Med 363(25):2434–244310.1056/NEJMra0912273 ↗
22. Kirpotin DB, Drummond DC, Shao Y, Shalaby MR, Hong K, Nielsen UB, Marks JD, Benz CC, Park JW (2006) Antibody targeting of long-circulating lipidic nanoparticles does not increase. Cancer Res 66(13):6732–674010.1158/0008-5472.CAN-05-4199 ↗
23. Lai JC, Lai MB, Jandhyam S, Dukhande VV, Bhushan A, Daniels CK, Leung SW (2008) Exposure to titanium dioxide and other metallic oxide nanoparticles induces cytotoxicity on human neural cells and fibroblasts. Int J Nanomedicine 3(4):533–545
24. Landoulsi J, Genet MJ, Richard C, El Kirat K, Pulvin S, Rouxhet PG (2008) Evolution of the passive film and organic constituents at the surface of stainless steel immersed in fresh water. J Colloid Interface Sci 318:278–28910.1016/j.jcis.2007.09.087 ↗
25. Li G, Hu Z, Yin H, Zhang Y, Huang X, Wang S, Li W (2013) A novel dendritic nanocarrier of polyamidoamine-polyethylene glycol-cyclic RGD for “smart” small interfering RNA delivery and in vitro antitumor effects by human ether-a-go–go-related gene silencing in anaplastic thyroid carcinoma cells. Int J Nanomedicine 8:1293–130610.2147/IJN.S41555 ↗
26. Mano SS, Kanehira K, Sonezaki S, Taniguchi A (2012) Effect of polyethylene glycol modification of TiO2 nanoparticles on cytotoxicity and gene expressions in human cell lines. Int J Mol Sci 13:3703–371710.3390/ijms13033703 ↗
27. Ninomiya K, Ogino C, Shema S, Sonoke S, Kuroda S, Shimizu N (2012) Targeted sonodynamic therapy using protein-modified TiO2 nanoparticles. Ultrason Sonochem 19:607–61410.1016/j.ultsonch.2011.09.009 ↗
28. Okuda-Shimazaki J, Takaku S, Kanehira K, Sonezaki S, Taniguchi A (2010) Effects of titanium dioxide nanoparticle aggregate size on gene expression. Int J Mol Sci 11(6):2383–239210.3390/ijms11062383 ↗
29. Park EJ, Yi J, Chung KH, Ryu DY, Choi J, Park K (2008) Oxidative stress and apoptosis induced by titanium dioxide nanoparticles in cultured BEAS-2B cells. Toxicol Lett 180:222–22910.1016/j.toxlet.2008.06.869 ↗
30. Riviere C, Wilhelm C, Cousin F, Dupuis V, Gazeau F, Perzynski R (2007) Internal structure of magnetic endosomes. Eur Phys J E 22(1):1–1010.1140/epje/e2007-00014-1 ↗
31. Rouxhet PG, Genet MJ (2011) XPS analysis of bio-organic systems. Surf Interface Anal 43(12):1453–147010.1002/sia.3831 ↗
32. Song M, Pan C, Li J, Zhang R, Wang X, Gu Z (2008) Blends of TiO2 nanoparticles and poly (N-isopropylacrylamide)-co-polystyrene nanofibers as a means to promote the biorecognition of an anticancer drug. Talanta 75:1035–104010.1016/j.talanta.2008.01.005 ↗
33. Spadavecchia J, Boujday S, Landoulsi J, Pradier CM (2011) nPEG-TiO2 nanoparticles: a facile route to elaborate nanostructured surfaces for biological applications. ACS Appl Mater Interfaces 3(7):2637–264210.1021/am200442r ↗
34. Spadavecchia J, Methivier C, Landoulsi J, Pradier CM (2013) Interaction of Zn(II) porphyrin with TiO2 nanoparticles: from mechanism to synthesis of hybrid nanomaterials. ChemPhysChem 14(11):2462–246910.1002/cphc.201300193 ↗
35. Wagner V, Dullaart A, Bock AK, Zweck A (2006) The emerging nanomedicine landscape. Nat Biotechnol 24(10):1211–121710.1038/nbt1006-1211 ↗
36. Wilhelm C, Billotey C, Roger J, Pons JN, Bacri JC, Gazeau F (2003) Intracellular uptake of anionic superparamagnetic nanoparticles as a function of their surface coating. Biomaterials 24:1001–101110.1016/S0142-9612(02)00440-4 ↗
37. Xu J, Sun Y, Huang J, Chen C, Liu G, Jiang Y, Zhao Y, Jiang Z (2007) Photokilling cancer cells using highly cell-specific antibody-TiO2 bioconjugates and electroporation. Bioelectrochemistry 71:217–22210.1016/j.bioelechem.2007.06.001 ↗
38. Xu M, Gao Y, Moreno EM, Kunst M, Muhler M, Wang Y, Idriss H, Wöll C (2011) Photocatalytic activity of bulk TiO2 anatase and rutile single crystals using infrared absorption spectroscopy. Phys Rev Lett 106(13):13830210.1103/PhysRevLett.106.138302 ↗
39. Yamaguchi S, Kobayashi H, Narita T, Kanehira K, Sonezaki S, Kudo N, Kubota Y, Terasaka S, Houkin K (2011) Sonodynamic therapy using water-dispersed TiO2-polyethylene glycol compound on glioma cells: comparison of cytotoxic mechanism with photodynamic therapy. Ultrason Sonochem 18:1197–120410.1016/j.ultsonch.2010.12.017 ↗
40. Yin ZF, Wu L, Yang HG, Su YH (2013) Recent progress in biomedical applications of titanium dioxide. Phys Chem Chem Phys 15(14):4844–485810.1039/c3cp43938k ↗
41. Zhang AP, Sun YP (2004) Photocatalytic killing effect of TiO2 nanoparticles on Ls-174-t human colon carcinoma cells. World J Gastroenterol 10(21):3191–319310.3748/wjg.v10.i21.3191 ↗