Cellular uptake and cytotoxicity of positively charged chitosan gold nanoparticles in human lung adenocarcinoma cells
Source abstract
Abstract
Cellular uptake, cytotoxicity, and mechanisms of cytotoxicity of the positively charged Au nanoparticles (NPs) were examined in A549 cells, which are one of the most characterized pulmonary cellular systems. Positively charged Au NPs were prepared by chemical reduction using chitosan. The dimension and surface charge of Au NPs were examined by transmission electron microscopy (TEM), dynamic light scattering, and zeta potential measurements. The uptake of Au NPs into A549 cells was also monitored using TEM and dark-field microscopy (DFM) and z-stack confocal microRaman spectroscopy. DFM live cell imaging was also performed to monitor the entry of chitosan Au NPs in real time. The cytotoxic assay, using both methylthiazol tetrazolium and lactate dehydrogenase assays revealed that positively charged Au NPs decreased cell viability. Flow cytometry, DNA fragmentation, real-time PCR, and western blot analysis suggest that positively charged chitosan Au NPs provoke cell damage through both apoptotic and necrotic pathways.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanoparticles: synthesis and applications · Graphene and Nanomaterials Applications · Nanoparticle-Based Drug Delivery
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (34)
Show source-provided references
1. Ahamed M, Akhtar MJ, Siddiqui MA, Ahmad J, Musarrat J, Al-Khedhairy AA, AlSalhi MS, Alrokayan SA (2011) Oxidative stress mediated apoptosis induced by nickel ferrite nanoparticles in cultured A549 cells. Toxicology 283:101–10810.1016/j.tox.2011.02.010 ↗
2. Akhtar MJ, Ahamed M, Kumar S, Siddiqui H, Patil G, Ashquin M, Ahmad I (2010) Nanotoxicity of pure silica mediated through oxidant generation rather than glutathione depletion in human lung epithelial cells. Toxicology 276:95–10210.1016/j.tox.2010.07.010 ↗
3. Arora S, Jain J, Rajwade JM, Paknikar KM (2008) Cellular responses induced by silver nanoparticles: in vitro studies. Toxicol Lett 179:93–10010.1016/j.toxlet.2008.04.009 ↗
4. Arvizo RR, Miranda OR, Thompson MA, Pabelick CM, Bhattacharya R, Robertson JD, Rotello VM, Prakash YS, Mukherjee P (2010) Effect of nanoparticle surface charge at the plasma membrane and beyond. Nano Lett 10:2543–254810.1021/nl101140t ↗
5. Auffan M, Rose J, Bottero JY, Lowry GV, Jolivet JP, Wiesner MR (2009) Towards a definition of inorganic nanoparticles from an environmental, health and safety perspective. Nat Nanotechnol 4:634–64110.1038/nnano.2009.242 ↗
6. Boisselier E, Astruc D (2009) Gold nanoparticles in nanomedicine: preparations, imaging, diagnostics, therapies and toxicity. Chem Soc Rev 38:1759–178210.1039/b806051g ↗
7. Choi SY, Jeong S, Jang SH, Park J, Park JH, Ock KS, Lee SY, Joo SW (2012) In vitro toxicity of serum protein-adsorbed citrate-reduced gold nanoparticles in human lung adenocarcinoma cells. Toxicol In Vitro 26:229–23710.1016/j.tiv.2011.11.016 ↗
8. Connor EE, Mwamuka J, Gole A, Murphy CJ, Wyatt MD (2005) Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity. Small 1:325–32710.1002/smll.200400093 ↗
9. Duceppe N, Tabrizian M (2010) Advances in using chitosan-based nanoparticles for in vitro and in vivo drug and gene delivery. Expert Opin Drug Deliv 7:1191–120710.1517/17425247.2010.514604 ↗
10. Founce TA, White J, Matthaei KI (2008) Integrated research into nanoparticle-protein corona: a new focus for safe, sustainable and equitable development. Nanomedicine 3:859–86610.2217/17435889.3.6.859 ↗
11. Goodman CM, McCusker CD, Yilmaz T, Rotello VM (2004) Toxicity of gold nanoparticles functionalized with cationic and anionic side chains. Bioconjug Chem 15:897–90010.1021/bc049951i ↗
12. Gratton SEA, Ropp PA, Pohlhaus PD, Luft JC, Madden VJ, Napier ME, DeSimone JM (2008) The effect of particle design on cellular internalization pathways. Proc Natl Acad Sci USA 105:11613–1161810.1073/pnas.0801763105 ↗
13. Haynes CL (2010) The emerging field of nanotoxicology. Anal Bioanal Chem 398:587–58810.1007/s00216-010-3972-5 ↗
14. Hu W, Peng C, Lv M, Li X, Zhang Y, Chen N, Fan C, Huang Q (2011a) Protein corona-mediated mitigation of cytotoxicity of graphene oxide. ACS Nano 5(5):3693–370010.1021/nn200021j ↗
15. Hu XY, Fang Q, Wang JS, Xie JQ, Chai BS, Li FQ, Cui X, Yang Y (2011b) Over-expression of aldehyde dehydrogenase-2 protects against H2O2-induced oxidative damage and apoptosis in peripheral blood mononuclear cells. Acta Pharmacol Sin 32(2):245–25210.1038/aps.2010.203 ↗
16. Jeong S, Choi SY, Park J, Seo JH, Park J, Cho KC, Joo SW, Lee SY (2011) Low-toxicity chitosan gold nanoparticles for small hairpin RNA delivery in human lung adenocarcinoma cells. J Mater Chem 21(36):13853–1385910.1039/c1jm11913c ↗
17. Kang B, Mackey MA, El-Sayed MA (2010) Nuclear targeting of gold nanoparticles in cancer cells induces DNA damage, causing cytokinesis arrest and apoptosis. J Am Chem Soc 132:1517–151910.1021/ja9102698 ↗
18. Khan JA, Pillai B, Das TK, Singh Y, Maiti S (2007) Molecular effects of uptake of gold nanoparticles in HeLa cells. ChemBioChem 8:1237–124010.1002/cbic.200700165 ↗
19. Lin J, Zhang H, Chen Z, Zheng Y (2010) Penetration of lipid membranes by gold nanoparticles: insights into cellular uptake, cytotoxicity, and their relationship. ACS Nano 4(9):5421–542910.1021/nn1010792 ↗
20. Liu S, Xu L, Zhang T, Ren G, Yang Z (2010) Oxidative stress and apoptosis induced by nanosized titanium dioxide in PC12 cells. Toxicology 267:172–17710.1016/j.tox.2009.11.012 ↗
21. Lynch I, Salvati A, Dawson KA (2009) What does the cell see? Nat Nanotechnol 4:546–54710.1038/nnano.2009.248 ↗
22. Mitchell DB, Santone KS, Acosta D (1980) Evaluation of cytotoxicity in cultured cells by enzyme leakage. J Tissue Cult Methods 6:113–11610.1007/BF02082861 ↗
23. Nel A, Xia T, Mädler L, Li N (2006) Toxic potential of materials at the nanolevel. Science 311:622–62710.1126/science.1114397 ↗
24. Pan Y, Leifert A, Ruau D, Neuss S, Bornemann J, Schmid G, Brandau W, Simon U, Jahnen-Dechent W (2009) Gold nanoparticles of diameter 1.4 nm trigger necrosis by oxidative stress and mitochondrial damage. Small 5(18):2067–207610.1002/smll.200900466 ↗
25. Patra HK, Banerjee S, Chaudhuri U, Lahiri P, Dasgupta AKr (2007) Cell selective response to gold nanoparticles. Nanomedicine 3:111–11910.1016/j.nano.2007.03.005 ↗
26. Pernodet N, Fang X, Sun Y, Bakhtina A, Ramakrishnan A, Sokolov J, Ulman A, Rafailovich M (2006) Adverse effects of citrate/gold nanoparticles on human dermal fibroblasts. Small 2:766–77310.1002/smll.200500492 ↗
27. Perumal OP, Inapagolla R, Kannan S, Kannan SM (2008) The effect of surface functionality on cellular trafficking of dendrimers. Biomaterials 29:3469–347610.1016/j.biomaterials.2008.04.038 ↗
28. Schaeublin NM, Braydich-Stolle L, Schrand AM, Miller JM, Hutchison J, Schlager JJ, Hussain SM (2011) Surface charge of gold nanoparticles mediates mechanism of toxicity. Nanoscale 3:410–42010.1039/c0nr00478b ↗
29. Simpson CA, Huffman BJ, Gerdon AE, Cliffel DE (2010) Unexpected toxicity of monolayer protected gold clusters eliminated by PEG-thiol place exchange reactions. Chem Res Toxicol 23:1608–161610.1021/tx100209t ↗
30. Uboldi C, Bonacchi D, Lorenzi G, Hermanns MI, Pohl C, Baldi G, Unger RE, Kirkpatrick CJ (2009) Gold nanoparticles induce cytotoxicity in the alveolar type-II cell lines A549 and NCIH441. Part Fibre Toxicol 6:18–2910.1186/1743-8977-6-18 ↗
31. Unfried K, Albrecht C, Klotz LO, Mikecz AV, Beck SG, Schins RPF (2007) Cellular responses to nanoparticles: target structures and mechanisms. Nanotoxicology 1:52–7110.1080/00222930701314932 ↗
32. Wei D, Qian W (2008) Facile synthesis of Ag and Au nanoparticles utilizing chitosan as a mediator agent. Colloid Surf B 62:136–14210.1016/j.colsurfb.2007.09.030 ↗
33. Yen HJ, Hsu SH, Tsai CL (2009) Cytotoxicity and immunological response of gold and silver nanoparticles of different sizes. Small 5:1553–156110.1002/smll.200900126 ↗
34. Zhang Y, Yang M, Portney NG, Cui D, Budak G, Ozbay E, Ozkan M, Ozkan CS (2008) Zeta potential: a surface electrical characteristic to probe the interaction of nanoparticles with normal and cancer human breast epithelial cells. Biomed Microdevices 10:321–32810.1007/s10544-007-9139-2 ↗